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Purif cation Expert

RNAprep Family

applied for RNA isolation from various samples

Member: 6

Sample type: animal, plant, bacteria, blood, FFPE, and micro samples

Feature: high purity, fast, safe and stable

Clinical Family

applied for DNA isolation from various clinical samples

Member: 13

Sample type: whole blood, blood clot, serum, plasma, buccal swab, tissue, cell, FFPE, stool, dry blood spot, amniotic fl uid, hair, virusFeature: personalized developed, broadly applied, high purity, safe and fast

Other Genomic DNA Isolation Family

applied for DNA isolation from other materials

Member: 10

Sample type: animal and plant tissue, plant powder, bacteria, virus, feedstuff, marine animal, GMO

plant, processed food.

Feature: personalized developed, broadly applied, high purity, safe and fast

Three Nucleic Acid Purifi cation Familiesprovide personalized solution for different experimental demands

Clinical Family

Fresh leaves of Arabidopsis thaliana, wheat, potato, tomato, rape, tobacco, rice, soy bean, corn

Corn white endosperm, wheat seed, Ormosia hosiei seed, and f lamentous fungi

Cotton leaves, mature rice leaves, Arabidopsis thaliana seed, pine needle, banana, Loquat leaves, potato tuber, apple, pear, watermelon, kiwi fruit, rose, tobacco, sea-buckthorn, lily

Cultured cell/bacteria

Tissues (liver, heart, lung, kidney, spleen, skin, etc.) and rat tail

Fresh/anticoagulant blood/blood clot/buffy coat/blood cells

Fresh/long-term stored FFPE slices

Serum/plasma/lymph

Microcuting frozen sample/ f brous tissue/animal tissue/animal cell

Animal tissue/cell

Materials not complex; limited polysaccharide & polyphenol; thin cell wall

Special secondary metabolites, diff cult to purify

High content of polysaccharide, polyphenol and other secondary metabolites

Cell wall of bacteria should be treated with lysozyme (RT401)

Tissues like muscle and skin should be grinded completely to get a good result

Blood is complex, should be purif ed at the earliest, or stored with specif ed blood protecting reagent (DP440 RNALock Reagent)

Low amount of samples, RNA will break into pieces when deparaff nization, RNA yield is low

Low copies of virus DNA

RNA is low in micro tissue and cell (low to 10 cells), diff cult to isolate

Precious sample

High purity: DP432 RNAprep Pure Plant KitNormal: DP405 TRNzol Reagent

High purity: DP432 RNAprep Pure Plant Kit (please ask Buffer HL for free)

High purity: DP441 RNAprep Pure Plant Kit (Polysaccharides&Polyphenolics-rich)Normal: DP437 RNAplant Plus Reagent

High purity: DP430 RNAprep Pure Cell/Bacteria KitNormal: DP405 TRNzol Reagent

High purity: DP431 RNAprep Pure Tissue KitNormal: DP405 TRNzol Reagent

High purity: DP433 RNAprep Pure Blood KitNormal: DP405 TRNzol Reagent

High purity: DP439 RNAprep Pure FFPE Kit

High purity: DP315-R TIANamp Virus RNA Kit DP315 TIANamp Virus DNA/RNA Kit

High purity: DP420 RNAprep Pure Micro Kit

High purity: DP422 DNA/RNA Isolation Kit DP423 DNA/RNA/Protein Isolation Kit

Sample type Examples Material features Purifi cation method

RNAprep Family

Feature:Fast: only 40-50 minHigh Purity: gDNA wipeout by pharmaceutical-grade DNase I; no genomic DNA remained; high-purity RNA is qualif ed for Real Time PCRSafe: No Phenol/Chloroform

—— applied for RNA isolation from various samples

Normal plantsample

Plant tissue with specialsecondarymetabolites

Plant tissue richin polysaccharide & polyphenol

Cultured cell/bacteria

Animal tissue

Blood

FFPE

Virus

Micro samples

Co-isolation ofnucleic acids

Professional & Dependable

Sample Volume (mg) RNA yield (μg)Banana fl esh 100 3-5Watermelon fl esh 100 1.5-2.4Apple fl esh 100 1.2-2Pear fl esh 100 1.2-2Sweet potato tubers 100 5.5-9Potato tubers 100 6-10White pine needles 100 15-20 Cotton leaves 100 20-25Rose leaves 100 20-25Loquat leaves 100 8-10Rice leaves 100 20-25

RNAprep Pure Plant Kit (DP432) ——Obtain high-purity total RNA from plant or fungi

RNAprep Pure Plant Kit (Polysaccharides & Polyphenolics-rich) (DP441)

——Solid choice for RNA purif cation from diff cult plant samples

Materials: 100 mg fl esh of banana, watermelon, apple and pear, 100 mg tuber of sweet potato and potato, 100 mg leaves of cotton, rose, loquat, and rice, 100 mg white pine needlesMethod: DP441 RNAprep Pure Plant Kit (Polysaccharides & Polyphenolics-rich) Elution volume: 30 μlRNA loading: 4-6 μlAgarose: 1%Electrophoresis: 6 V/m, 30 minM: TIANGEN Marker IIIResult: using DP441 could obtain intact RNA with high yield, and no DNA contamination

M watermelon banana apple pear sweet potato potato white pine cotton rose loquat rice needles

Sample Volume RNA yield

Yeast 7×107cells 30-100 μg

Fresh tabacco leaves 100 mg 73 μg

Fresh Arabidopsis Thaliana leaves

100 mg 35 μg

Fresh corn leaves 100 mg 25 μg

Fresh tomato leaves 100 mg 65 μg

Materials: Atenia cordifolia leaves (80 mg)Method: DP432 RNAprep Pure Plant KitElution volume: 100 μlRNA loading: 2-4 μlAgarose: 1%Electrophoresis: 6 V/m, 30 min

RNAprep Pure Tissue Kit (DP431) ——Isolate up to 100 μg total RNA from animal tissue

RNAprep Pure Cell/ Bacteria Kit (DP430)

——Isolate high qualif ed total RNA from cultured cells/bacteria

RNAprep Pure Blood Kit (DP433)

——Isolate total RNA from fresh whole blood

TRNzol Reagent (DP405)

——Obtain high qualif ed RNA from different sample types

1 2 3 4 5 6 7 8 9M

Materials: embryo (13 days, 20 mg), kidney (15 mg), liver (10 mg), spleen (15 mg), thymus gland (10 mg), lung (20 mg)Method: DP431 RNAprep Pure Tissue Kit Elution volume: 100 μlRNA loading: 2-4 μlAgarose: 1%Electrophoresis: 6 V/cm, 30 minM: TIANGEN DNA Marker IIILane 1-2: Embryo (13 days) Lane 3: Kidney Lane 4-6: Liver Lane 7: SpleenLane 8: Thymus gland Lane 9: Lung

RNA yield for different rat tissues

Materials: Human Jurkat Cells (1x106)Method: DP430 RNAprep Pure Cell/Bacteria KitElution volume: 50 μlRNA loading: 2-4 μlAgarose: 1%Electrophoresis: 6 V/cm, 30 min

Materials: Human Jurkat Cells (1x106)Method: DP405 TRNzol Reagent from TIANGEN and similar product from Supplier AElution volume: 50 μlRNA loading: 2-4 μlM: TIANGEN Marker IIIAgarose: 1%Electrophoresis: 6 V/cm, 30 min1,2,3: RNA isolated by TIANGEN TRNzol4,5,6: RNA isolated by similar product from Supplier A

Materials: fresh rat blood (100 μl)Method: DP433 RNAprep Pure Blood KitElution volume: 50 μl RNA loading: 4-6 μl M: TIANGEN DNA Marker III Agarose: 1%Electrophoresis: 6 V/cm, 30 min

M 1 2 3 4 5 6 7 8

M 1 2 3 4 5 6 Sample types Yield (μg)

1 g rice leaves 100-200

1 mg rat liver 6-10

10 6 cultured cells 5-10

1 ml E. coli culture 2-10

1 ml human whole blood 3-5

yieldμg

embryo kidney liver spleen thymus lung (13 days) gland

Professional & Dependable

RNAprep Pure FFPE Kit (DP439) ——Isolate and purify total RNA from FFPE sample

TIANamp Virus DNA/RNA Kit (DP315)

——Isolate virus DNA and RNA from plasma, serum and cell-free fl uid

RNAstore Reagent (DP408) ——Non-frozen reagent for preservation of intact RNA in samples

RNALock Reagent (DP440) ——Preserve fresh whole blood for nucleic acid purif cation

M 1 2 3 4

Materials: FFPE from rat liver (around 15 mg)Method: DP439 RNAprep Pure FFPE Kit Elution volume: 50 μl RNase-Free ddH2ORNA loading: 8 μlM: TIANGEN Marker IIIAgarose: 1%Electrophoresis: 6 V/cm, 20 min1-4: Rat liver FFPE sample

Materials: serum sample containing Hepatitis E virus (5x106, 5x105, 5x104, 5x103, 5x102 copies/ml)Method: DP315 TIANamp Virus DNA/RNA Kit & FP302 Quant qRT-PCR Kit (SYBR Green) Result: virus RNA purified by DP315 could be 5x103 copies/ml, virus recovery yield could be around 85%.

Materials: rat liver (15 mg)Method: 0.5 g rat liver was stored by DP408 at 37℃, room temperature and 4℃ respectively. Purify RNA from 15 mg rat liver taken from above samples respectively by DP405 TRNzol Reagent. Elution volume: 100 μl RNA loading: 2-4 μlC (positive control): RNA purif ed from tissues directly stored at -80℃Agarose: 1%Electrophoresis: 6 V/cm, 30 min

Materials: fresh rat blood (100 ul)Method: RNA purif cation from RNALock Reagent stored blood samples by DP433 RNAprep Pure Blood Kit Elution volume: 50 μl RNA loading: 4-6 μlM: TIANGEN Marker IIIAgarose: 1%Electrophoresis: 6 V/cm, 30 minLane 1-2 (positive control): RNA purif ed from fresh bloodLane 3-4: RNA purif ed from blood sample stored at 15-25℃ for 2 daysLane 5-6: RNA purif ed from blood sample stored at 4-8℃ for one weekLane 7-8: RNA purif ed from blood sample stored at -20℃ for six months

1 2 1 2 1 2 1 2M 1 2 3 4 5 6 7 8

C 1day.37℃ 1week.RT 4weeks.4℃control 15-25℃ 4-8℃ -20/-70℃

RNA sample preservation

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Clinical FamilySince 2007, more than 550,000 preps of blood genomic DNA extraction for the China Marrow Donor Program (CMDP)Since 2008, more than 5,000,000 rxns of virus detection for Flu MonitoringSince 2010, more than 600,000 preps of medical sample testing for Prenatal and Newborn ScreeningSince 2013, provided automated nucleic acid extraction solutions for Largest Healthy Population Sample Library in ChinaIn 2013, provided more than 100,000 rxns of microbial detection for the Global Tuberculosis Screening Program

Cultured cells, cancer cells, cancer tissue, mucosa, tissues (liver, heart, brain, lung, kidney, spleen, etc), fbroblast, rat tail etc.

Fresh/anticoagulant frozen blood/blood clot/buffy coat/blood cells

Blood clot

Fresh/long-term stored paraffn sections

Buccal swab

Fresh or dry stool

Virus

Dried blood spots, chewing gum, buccal swab, hair/feather, nail, saliva, seminal stain, mouth wash, plasma/serum

Fresh or frozen sample,DNA is rich,Low level of other contaminant,Easy to purify

DNA content is relatively high in fresh and anticoagulant blood, yield will be lower for blood clot samples

Hard to disrupt;severe genomic DNAdegradation

Low sample volume; DNA will be disrupted during paraffn removing; DNA yield is low and fragment is short, cannot be stored for long term

Low sample volume and DNA; diffcult to enrich sample

Much contaminant, uneasy to remove, will inhibit downstream experiment

For DNA/RNA virus, usespecialized enrichment material

Low amount of samples and DNA; diffcult to enrich sample

For long-term storage: [spin column] DP304 TIANamp Genomic DNA KitFor fast detection: KG203 TIANcombi DNA Lyse & Det PCR Kit

For long-term storage: [precipitation] DP319 RelaxGene Blood DNA System[spin column]DP318 TIANamp Blood DNA KitDP332 TIANamp Blood DNA Midi KitDP333 TIANamp Blood DNA Maxi KitDP314 TIANamp N96 Blood DNA KitFor fast detection: KG203 TIANcombi DNA Lyse & Det PCR Kit

For long-term storage: DP318 TIANamp Blood DNA Kit and Liquefaction Columns CX1

Xylene based: DP331 TIANamp FFPE DNA KitNot xylene based: DP330 TIANquick FFPE DNA Kit

For long-term storage: DP322 TIANamp Swab DNA Kit

For long-term storage: DP328 TIANamp Stool DNA Kit

For long-term storage: DP315 TIANamp Virus DNA/RNA Kit

For long-term storage: [spin column] DP316 TIANamp Micro DNA Kit

Sample type Examples Material features Purification method

Cultured cells/animal tissue

Blood

Blood clot

FFPE

Buccal swab

Stool

Virus

Micro sample

Professional & Dependable

Sample Volume DNA yield (μg)

Mammalian Blood 100-500 μl 3-10

Bird and Amphibian Blood 5 μl 5-10

Cultured Cells 106-107 Cells 5-30

Animal Tissues 30 mg 10-30

Mouse Tail 1.2 cm（tip） 10-25

Rat Tail 0.6 cm（tip） 20-40

TIANamp Genomic DNA Kit (DP304)

——Silica membrane based high quality DNA purifcation from blood/cell/tissue

TIANamp Micro DNA Kit (DP316) ——Genomic DNA purifcation from small volume samples including whole

blood, serum/plasma, forensic materials, blood stain, swab, etc.

DNA yield with different plant tissues

M

Materials: 300 μl blood Method: DP304 TIANamp Genomic DNA Kit Elution volume: 100 μl DNA loading: 3 μlM: TIANGEN Marker DNA/Hind III

Materials: 200 μl plasma/serumMethod: DP316 TIANamp Micro DNA Kit & qPCRElution volume: 50 μl DNA template: 4 μlqPCR system: 20 μlDetection primer: beta globin

Materials: dry blood spotMethod: DP316 TIANamp Micro DNA Kit & qPCRElution volume: 80 μl DNA template: 5 μlqPCR system: 20 μlDetection primer: HS4

TIANamp Blood DNA Kit (DP318) ——Silica membrane based high quality DNA purifcation from blood samples

DNA yield with different plant tissues

DNA yield varies according to white cells qty and storage condition of blood sample

Materials: 200 μl, 600 μl and 1 ml of blood Method: DP318 TIANamp Blood DNA Kit Elution volume: 100 μl DNA loading: 3 μlM: TIANGEN Marker DNA/Hind III

M 200 μl 600 μl 1 ml

Sample Volume (μg) DNA yield (μg)Mammalian Blood 100-500 3-10 500-1000 4-30Bird and Amphibian Blood 5-20 5-40 Blood clot 200-500 1-8 500-1000 8-15

Other Genomic DNA Isolation Family

specially developed for agriculture, microbe, marine, environment areas

From 2007, provided feed detection kits for the Prevention and Control of BSE, and developed Industry Standards with Ministry of Agriculture;In 2009, TIANGEN products were aboard the Xuelong, Chinese Research vessel, for the Antarctic Research Expedition;From 2010, several products were developed for GMO Research to satisfy the requirements of agricultural research laboratories.If you are doing research about plant leaves, seeds, fruits, microbes, yeast, marine animals, feedstuff, processed food, soil... please see below

Normal plant sample

High-polysaccharide & polyphenol plant/plant powder/seed/fungus/moss

Cultured cell/animal tissue

Bacteria (G+/G-)

Yeast

Processed food

Marine animals

Feedstuff

Soil

Fresh leaves of Arabidopsis thaliana, wheat, potato, rape, tobacco, rice, soy bean, corn

Strawberry, cotton, hot pepper, pine tree, potato, tomato, soy bean, tree leaves, fern plant leaves, rosaceae plant leaves, moss, fungus, plant leaves powder and plant seeds powder

Cultured cell, cancer cell, cancer tissue, mucosa, tissues (liver, heart, lung, kidney, spleen), desmocyte, rat tail

Bacteria (G+/G-)

Yeast

Crisps/ bean paste/ketchup/soy sauce/chips/biscuits/instant noodles/tofu/dried tofu/fermented bean curd/soybean milk/steamed bread/vermicelli

Tissue and glands of Fish, shrimps, shellf sh, crabs

Animal feedstuff and raw material of feedstuff

Dust, forest soil, farmland soil, potting soil

Materials not complex; limited polysaccharide & polyphenol; thin cell wall

High content of polysaccharide, polyphenol and starch; thick cell wall

Fresh or frozen sample, DNA is rich; low contaminants; easy to isolate

Cell wall of G+ bacteria is diff cult to lysis

Cell wall of yeast is diff cult to lysis

After fermentation, gDNA will be degraded a lot, DNA is in short fragment and low in content

Due to special features of marine animal, protein, lipid and other organic contaminants should be removed exclusively

Complex content, low content of cattle source ingredient

Much contaminants, uneasy to remove, inhibit downstream experiment;low yield of DNA

For long-term storage:[spin column] DP320 DNAsecure Plant Kit[precipitation] DP321 DNAquick Plant SystemFor fast detection: KG203 TIANcombi DNA Lyse & Det PCR Kit

For long-term storage: [spin column] DP305 Plant Genomic DNA KitFor fast detection: KG203 TIANcombi DNA Lyse & Det PCR Kit

For long-term storage: [spin column] DP304 TIANamp Genomic DNA KitFor fast detection: KG203 TIANcombi DNA Lyse & Det PCR Kit

For long-term storage: [spin column] DP302 TIANamp Bateria DNA KitFor fast detection: KG203 TIANcombi DNA Lyse & Det PCR Kit

For long-term storage:[spin column] DP307 TIANamp Yeast DNA KitFor fast detection: KG203 TIANcombi DNA Lyse & Det PCR Kit

For long-term storage: [spin column] DP326 GMO Food DNA Extraction Kit

For long-term storage: [spin column] DP324 TIANamp Marine Animals DNA Kit

For long-term storage:[spin column] DP323 TIANamp Feedstuff Animal DNA Kit

For long-term storage: [spin column] DP336 TIANamp Soil DNA Kit

Sample type Examples Material features Purifi cation method

Professional & Dependable

DNAsecure Plant Kit (DP320)

——For quick genomic DNA purifcation from a wide range of plant samples

TIANamp Plant DNA Kit (DP305)

——For genomic DNA purifcation from powder or polysaccharide/ polyphenol rich plants

DNA yield with different plant tissues

DNA yield

DNA yield with different plant tissues

Materials: plant leaves (100 mg)1: fresh soy bean leaves2: fresh tabacco leaves3: fresh wheat leaves4: fresh corn leavesMethod: DP320 DNAsecure Plant KitElution volume: 100 μl DNA loading: 3 μlAgarose: 1%Electrophoresis: 6 V/cm, 20 minM: TIANGEN Marker DNA/Hind III

Materials: plant leaves (100 mg)1: fresh tomato leaves2: fresh cotton leaves3: fresh tea leaves4: fresh strawberry leavesMethod: DP305 TIANamp Plant DNA KitElution volume: 100 μl DNA loading: 3 μlAgarose: 1%Electrophoresis: 6 V/cm, 20 minM: TIANGEN Marker DNA/Hind III

4 3 2 1 M

M 1 2 3 4

Plant Plant Average materials weight DNA yield OD260 / OD280

Arabidopsis thaliana 100 mg 3-4 μg 1.7 - 1.9

Wheat 100 mg 25-30 μg 1.7 - 1.9Pine tree 100 mg 25-30 μg 1.7 - 1.9Potato 100 mg 4-6 μg 1.7 - 1.9Tomato 100 mg 10-15 μg 1.7 - 1.9Rape 100 mg 2-4 μg 1.7 - 1.9Tabacco 100 mg 20-25 μg 1.7 - 1.9Rice 100 mg 10-25 μg 1.7 - 1.9Soy bean 100 mg 20-30 μg 1.7 - 1.9Corn 100 mg 20-30 μg 1.7 - 1.9

Bacteria G- bacteria G+ bacteria

type (e.g. E. coli ) (e.g. Staphylococcus epidermidis)

cells 2×108 cells/ml 3.5×108 cells/ml

culture volume 1 ml 1 ml

DNA yield 15-20 μg 6-13 μg

OD260 / OD280 1.7-1.9 1.7-1.9

Plant Plant Average materials weight DNA yield OD260 / OD280

Wheat 100 mg 25-30 μg 1.7 - 1.9Pine tree 100 mg 25-30 μg 1.7 - 1.9Potato 100 mg 4-6 μg 1.7 - 1.9Tomato 100 mg 10-15 μg 1.7 - 1.9Strawberry 100 mg 10-15 μg 1.7 - 1.9Tabacco 100 mg 20-25 μg 1.7 - 1.9Rice 100 mg 10-25 μg 1.7 - 1.9Soy bean 100 mg 20-30 μg 1.7 - 1.9Rice 100 mg 20-30 μg 1.7 - 1.9Cotton 100 mg 10-25 μg 1.7 - 1.9

TIANcombi DNA Lyse & Det PCR Kit (KG203)

——DNA Extraction from Plant, Bacteria and Tissue Samples Followed by PCR

TIANamp Bacteria DNA Kit (DP302)

——Simple and fast genomic DNA purifcation from gram-negative and gram-positive bacteria

1: positive control (genomic DNA as template)2: leaves 3: seeds4: NTCM: TIANGEN D2000

1: positive control (genomic DNA as template)2: 10 μl blood 3: 20 μl blood 4: 30 μl blood5: 40 μl blood 6: 50 μl blood 7: NTCM: TIANGEN D2000

1: positive control (genomic DNA as template)2: testing sample3: NTCM: TIANGEN D2000

1: positive control (genomic DNA as template)2: tail 3: liver 4: lung 5: NTCM: TIANGEN D2000

Rice EDTA anticoagulant Bacteria Animal tissue M 1 2 3 4 M 1 2 3 4 5 6 7 M 1 2 3 M 1 2 3 4 5

TIANGEN, a leading biotechnology company, provides customized molecular biology reagents and professional technical services to customers in the life science research field.Our comprehensive product portfolio includes technologies for genomic DNA extraction, RNA isolation, plasmid purification, PCR, RT-PCR and qRT-PCR, DNA markers, protein reagents as well as lab essential instrumentations. TIANGEN maintains a strict “Customer First” mentality and is dedicated to a continuous improvement in quality to fulfill our promise of best-in-class products and services.

TIANGEN PROFILE

In 2010, TIANGEN was awarded the ISO 9001:2008 certification by the Germany’s TÜV Rheinland. TIANGEN’s standardized production process, state-of-the-art production facilities and outstanding quality control system make our products a reliable partner to researchers.

Best PP materials, selected from over 300 suppliers in the world.

Absorption membrane that have international leading quality, guarantees the best efficiency and stability.

2007

2008

2009 2013

2010

2011

2012

J Bone Miner ResStem Cells

GeneInt J Cancer

TIANGEN, a leading biotechnology company, provides customized molecular biology reagents and professional technical services to customers in the life science research field.Our comprehensive product portfolio includes technologies for genomic DNA extraction, RNA isolation, plasmid purification, PCR, RT-PCR and qRT-PCR, DNA markers, protein reagents as well as lab essential instrumentations. TIANGEN maintains a strict “Customer First” mentality and is dedicated to a continuous improvement in quality to fulfill our promise of best-in-class products and services.

TIANGEN PROFILE

In 2010, TIANGEN was awarded the ISO 9001:2008 certification by the Germany’s TÜV Rheinland. TIANGEN’s standardized production process, state-of-the-art production facilities and outstanding quality control system make our products a reliable partner to researchers.

Best PP materials, selected from over 300 suppliers in the world.

Absorption membrane that have international leading quality, guarantees the best efficiency and stability.

2007

2008

2009 2013

2010

2011

2012

J Bone Miner ResStem Cells

GeneInt J Cancer

About Percepta

Founded in 2005 in Carlsbad, California Percepta is a specialized consultancy providing market assessment,

product development and pricing, brand assessment and development, market planning, ad concept and message

testing and due diligence services to life science research suppliers globally.

LINK:

http://www.businesswire.com/news/home/20130430005335/en/%3Cspan%20%20class='tip_s'%3ETiangen%3C/

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TIANGEN BIOTECH (BEIJING) CO,LTD.Address: Building C7-3, Zhongguancun Dongsheng Science Park, No. 66 Xixiaokou Road Haidian District, Beijing, China.Post Code: 100192 Website: www.tiangen.comE-mail: people@tiangen.com Tel: +86 10 59822688