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PROGRAM PROGENITOR CELL TRANSLATIONAL CONSORTIUM (PCTC) GERMAN CENTER FOR CARDIOVASCULAR SCIENCE (DZHK)

CARDIOVASCULAR BIOENGINEERING (CVBE) SYMPOSIUM 2020

Location: German Primate Center - Kellnerweg 4 - 37077 Göttingen, Germany ………………………………………………………………………………………………………………

THURSDAY, May 7, 2020 Registration & Coffee 9:00 – 11:00 Welcome Address 11:15 – 11:30

Stefan Treue, PhD Director, German Primate Center

Jay Zhang, MD, PhD Conference Chair (PCTC), UAB | The University of Alabama at Birmingham

Thomas Eschenhagen, MD Chair, German Center for Cardiovascular Research (DZHK)

Gerd Hasenfuß, MD Chair, Heart Center Göttingen

MODERATORS: Rabea Hinkel, DVM; Wolfram Zimmermann, MD

11:30 – 12:00 PM KEYNOTE LECTURE

Xenografts as an Alternative to Heart Transplantation Bruno Reichart, MD Walter Brendel Centre of Experimental Medicine LMU Munich, German

12:00 – 2:00 Lunch &Guided Tour - German Primate Center

Session I - Lessons from Large Animal Models MODERATORS: Rabea Hinkel, DVM; Wolfram Zimmermann, MD 2:00 – 2:20 PM

iPS-CM: Generation, Selection, and Delivery Keiichi Fukuda, MD, PhD Professor Department of Cardiology Keio University, Japan

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2:20 – 2:40 PM Controlling Cardiomyocyte Implant Induced Arrhythmia Michael Laflamme, MD, PhD Senior Scientist, McEwen Stem Cell Institute Robert McEwen Chair in Cardiac Regenerative Medicine, University Health Network Staff Pathologist, University Health Network Associate Professor, Laboratory Medicine and Pathobiology, University of Toronto

2:40 – 3:00 PM

Experience in Monkey Allo- and Autograft Models Yuji Shiba, MD, PhD Head of the Department of Biotechnology and Biomedical Engineering Academic Assembly Associate Professor, Institute of Medicine Institute for Biomedical Sciences Shinshu University, Japan

3:00 – 3:20 PM

NHP Models of Engineered Heart Repair to Inform Clinical Trials Wolfram-Hubertus Zimmermann, MD Director, Institute of Pharmacology and Toxicology University Medical Center Göttingen

3:20 – 3:40 PM

Translational Studies in Porcine Models of Diabetes Rabea Hinkel, DVM Professor, Laboratory Animal Science German Primate Center Göttingen, Germany

3:40 – 4:00 AM

Induced Cardiac Regeneration by Cell Cycle Activation Mauro Giacca, MD, PhD Professor, School of Cardiovascular Medicine King’s College London

________________________________________________________________________ 4:00 – 4:20 PM Coffee Break _____________________________________________________________________________

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Session II - Late Translational Studies

MODERATORS: Joseph Wu / Christian Kupatt 4:20 – 4:40 PM

miR Therapeutics for Heart Repair Stefanie Dimmeler, PhD Professor, Cardiovascular Institute, Frankfurt

4:40 – 5:00 PM Experience with Cell Sheet Therapy Yoshiki Sawa, MD Professor of Surgery Department of Cardiovascular Surgery Dean, Osaka University Graduate School of Medicine and Faculty of Medicine Osaka, Japan

5:00 – 5:20 PM

Novel strategies for cardiac regeneration and repair Eldad Tzahor, PhD Professor, Head, Department of Molecular Cell Biology Weizmann Institute of Science, Israel

5:20 – 5:40 PM Finding an Appropriate Dose for Engineered Heart Tissue Florian Weinberger, MD Group Leader in the Institute of Experimental Pharmacology and Toxicology University Medical Center Hamburg-Eppendorf

5:40 – 6:00 PM

Regulation of Heart Rate and Rhythm by CamKII Mark Edward Anderson, MD, PhD Director, Department of Medicine Johns Hopkins University School of Medicine

6:00 – 6:20 PM From ESCORT to EXOSOMES Philippe Menasché, MD, PhD Professor of Thoracic and Cardiovascular Surgery, University of Paris Descartes Professor of Biomedical Engineering, UAB | The University of Alabama at Birmingham

7:30 PM Faculty Dinner - Bullerjahn

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PROGRAM PROGENITOR CELL TRANSLATIONAL CONSORTIUM (PCTC) GERMAN CENTER FOR CARDIOVASCULAR SCIENCE (DZHK)

CARDIOVASCULAR BIOENGINEERING (CVBE) SYMPOSIUM 2020

Location: Pauliner Kirche, Göttingen, Germany ………………………………………………………………………………………………………………

FRIDAY, May 8 8

Welcome Address 8:15 – 8:30

Wolfram Zimmermann, MD Conference Chair (DZHK), Institute of Pharmacology and Toxicology University Medical Center Göttingen, Germany.

Victor J. Dzau, MD President, the National Academy of Medicine

Dennis Buxton, PhD Director, Basic and Early Translational Research Program, NHLBI Division of Cardiovascular Sciences

Michael L. Terrin, MDCM, MPH NHLBI Progenitor Cell Biology Consortium (PCBC) Administrative Coordinating Center, PI

Session III: Learning from Development 8:30 – 10:30 AM Chairs: David Elliot / Laura Zelarayan 8:30 – 8:50 AM

Transcriptional Dysregulation in Congenital Heart Failure David Elliot, PhD Australian Regenerative Medicine Institute, Monash University

8:50 – 9:10 AM

Derivation and Use of Epicardial Lineages from hPSCs Sanjay Sinha, MBBCh, PhD British Heart Foundation Senior Research Fellow, Wellcome-MRC Cambridge Stem Cell Institute University of Cambridge

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9:10 – 9:30 AM

Cell lineage diversification and Cell Competition during heart development and repair Miguel Torres, PhD Professor, Department of Cell and Developmental Biology Centro Nacional de Investigaciones Cardiovasculares (CNIC)

9:30 – 9:50 AM

Dissecting the Fate of Cardiac Progenitor Cells Thomas Braun, MD, PhD Director, Max-Planck-Institute of Heart and Lung Research, Bad Nauheim

9:50 – 10:10 PM

Cardiac Lymphatic System Paul Riley, PhD BHF Professor of Regenerative Medicine Chair of Development and Cell Biology Oxford University, UK

_______________________________________________________________________________ Coffee Break 10:10 – 10:30 AM ______________________________________________________________________________________ Session IV: Going 3D 10:30 AM – 12:10 PM Chairs: Jay Zhang / Thomas Eschenhagen 10:30 – 10:50 AM

Making LV Remuscularization Happen: using Engineered Cardiac Muscle Patch Jay Zhang, MD, PhD Chair, Department of Biomedical Engineering T. Michael and Gillian Goodrich Endowed Chair of Engineering Leadership Professor of Medicine, of Engineering School of Medicine, School of Engineering UAB | The University of Alabama at Birmingham

10:50 – 11:10 AM

Engineering Heart and Skeletal Muscle with Advanced Maturation Malte Tiburcy, MD Group Leader - Engineered Disease Models Institute of Pharmacology and Toxicology University Medical Center Göttingen

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11:10 – 11:30 AM Tissue-engineered Therapeutics for Heart Repair Nenad Bursac, PhD Professor of Biomedical Engineering Professor of Cell Biology Associate Professor of Medicine Co-director, Regeneration Next Duke University

11:30 – 11:50 PM 3D Printing of the Heart Tal Dvir, PhD Professor, Department of Molecular Microbiology and Biotechnology and the Center for Nanoscience and Nanotechnology The George S. Wise Faculty of Life Sciences Tel Aviv University

11:50 – 12:10 PM

PLN p.Arg14del: Disease modelling with hiPSC-cardiomyocytes Arne Hansen, MD Professor, Institute of Pharmacology and Toxicology University Medical Center Hamburg-Eppendorf, Germany

____________________________________________________________________________ 12:10 – 3:00 PM Poster Viewing with Lunch (Posters will be at display on May 8th and 9th) _____________________________________________________________________________ Session V - Early Career Researcher Presentations – selected from submitted abstracts Moderators: Joel L. Berry / Susanne Lutz

Rapid Fire Presentation (5 min presentation + 2 min discussion) 3:00-3:07 PM TBD 3:07-3:14 PM TBD 3:14-3:21 PM TBD 3:21-3:28 PM TBD 3:28-3:35 PM TBD 3:35-3:42 PM TBD 3:42-3:49 PM Panel Discussion: Chong, Fukuda, Liaw, Lutz, Zhang, Zimmermann

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_____________________________________________________________________________ 4:00 – 4:20 PM Coffee Break _____________________________________________________________________________ Session VI: Biomaterials and Tissue Engineering 4:20 – 6:00 PM Chairs: Joel L. Berry / Susanne Lutz 4:20 – 4:40 PM

Mechanobiology of iPSC-derived Cardiovascular Progeny Adam Engler, PhD Associate Professor of Bioengineering University of California San Diego

4:40 – 5:00 PM

Brenda Ogle, PhD Bioprinting of a Cardiogenic Extracellular Environment Professor and Chair, Department of Biomedical Engineering Director, Stem Cell Institute University of Minnesota – Twin Cities, Minneapolis, MN US

5:00 – 5:20 PM Stem Cells for Vascular Regeneration and Remodeling Song Li, PhD Chancellor Professor and Department Chair Department of Biomedical Engineering Department of Medicine University of California – Los Angeles (UCLA)

5:20 – 5:40 PM Biofabrication of Human Pancreatic Islets from Pluripotent Stem Cells Keiming Ye, PhD Professor and Chair, Department of Biomedical Engineering Director, Center of Biomanufacturing for Regenerative Medicine Binghamton University State University of New York (SUNY)

5:50 – 6:00 PM

Engineered Heart and Brain Interface Patapia Zafeiriou, PhD Institute of Pharmacology and Toxicology University Medical Center Göttingen

7:00 PM Conference Dinner / YI-Award Presentation - APEX

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Saturday, May 9 Session VII - Heart Failure – Mechanisms and Targets 8:30 – 10:30 AM Chairs: Reza Ardehali / Gerd Hasenfuß 8:15 – 8:50 AM KEYNOTE-LECTURE

The Future of Heart Failure Therapy / Paracrine Mechanism of Stem Cell Therapy Victor J. Dzau, MD Professor of Medicine, Duke University President, National Academy of Medicine

8:50 – 9:10 AM

Targeting Iron Deficiency Hossain Ardehali, MD, PhD Professor, Feinberg Cardiovascular Research Institute Department of Medicine Feinberg School of Medicine Northwestern University

9:10 – 9:30 AM

Regulatory Principles of Cardiac Proteins on a Global Scale Peipei Ping, PhD Professor of Physiology, Medicine/Cardiology, and Bioinformatics Director, NIH BD2K Center of Excellence at UCLA Director, NIH BD2K Centers-Coordination Center at UCLA UCLA

9:30 – 9:50 AM

Novel Strategies in Targeting GPCR Signaling Pathways Howard A. Rockman, MD Edward S. Orgain Professor of Cardiology Professor of Medicine, Cell Biology, and Molecular Genetics Director, Cardiovascular Research Center Duke University Medical Center

9:50 – 10:10 PM

Cardiac Regeneration Strategies: Cells and Matrix James Chong, MBBS, PhD Associate Professor University of Sydney Head, Cardiac Regeneration Laboratory; Faculty, Westmead Institute for Medical Research Cardiologist, Westmead Hospital Hon. Faculty, Victor Chang Cardiac Research Institute, Sydney, Australia

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10:10 – 10:30 PM

NO in the Heart Failure Stefan Janssens, MD Professor of Medicine Head of Cardiology, Department of Cardiovascular Sciences Centre for Molecular and Vascular Biology, Leuven, Belgium

_____________________________________________________________________________ 10:30 – 10:50 Coffee Break _____________________________________________________________________________ Session VIII - Heart Failure – Novel Strategies to Repair the Heart 10:50 – 12:50 AM Chairs: Victor Dzau / Thomas Braun 10:50 – 11:10 AM

Proliferating Cardiomyocytes: When and How? Loren Field, PhD Professor of Cellular & Integrative Physiology, Pediatrics, Medicine, Physiology, and Biophysics Krannert Institute of Cardiology Indiana University

11:10 – 11:30 AM

Nitrosative Stress in Heart Failure with Preserved Ejection Fraction Joseph Hill, MD, PhD Professor & James T. Willerson, MD Distinguished Chair in Cardiovascular Diseases Frank M. Ryburn Jr. Chair in Heart Research Department of Internal Medicine, Division of Cardiology Department of Molecular Biology UT Southwestern Medical Center

11:30 – 11:50 AM

Transforming Scar in Heart Muscle Masaki Ieda, MD, PhD Chair, Department of Cardiology, Professor of Medicine University of Tsukuba, Japan

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11:50 – 12:10 PM HDAC Manipulation in Heart Failure Johannes Backs, MD DZHK-Professor Director, Department of Molecular Cardiology and Epigenetics University of Heidelberg, Germany

12:10 – 12:30 AM Redox Regulation of Cardiomyocyte Renewal Hesham A. Sadek, MD, PhD J. Fred Schoellkopf, Jr. Chair in Cardiology Professor of Cardiology Associate Director for Cardiac Regeneration, Center for Regenerative Science & Medicine UT Southwestern Medical Center

12:30 – 12:50 PM

Non-coding RNA in Cardiac Regeneration and Protection Da-Zhi Wang Department of Pediatrics Boston Children's Hospital

_______________________________________________________________ 12:50 – 1:30 PM Lunch ___________________________________________________________________ Session IX: Genome Editing 1:30 – 3:10 PM Chairs: Loren Field / Tim Kamp 1:30 – 1:50 PM

Stem Cells and Genomics for Precision Medicine Joseph C Wu, MD, PhD Simon H. Stertzer, MD, Professor and Professor of Radiology Department of Medicine – Cardiovascular Medicine Stanford Medicine

1:50 – 2:10 PM

CRISPR/Cas-based Tuning Gene Expression Laura Zelaravan, PhD Department of Pharmacology and Toxicology Universitätsmedizin Göttingen

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2:10 – 2:30 PM

Transcriptional Regulation of Maturation William Pu, M.D. Professor of Pediatrics, Harvard Medical School Department of Cardiology, Boston Children’s Hospital

2:30 – 2:50 PM

Repairing DMD in Pigs Christian Kupatt, MD Professor, Clinic for Internal Medicine, Cardiology Technical University Munich, Germany

2:50 – 3:10 PM

Epigenetic Modification of Gene Expression Elisabeth Zeisberg, MD Department of Cardiology and Pneumology Universitätsmedizin Göttingen

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3:10 – 3:30 PM Coffee Break _____________________________________________________________________________ Session X: Cell-Cell Cross-Talk 3:30 – 5:30 PM Chairs: Nenad Bursac / Rabea Hinkel 3:30 – 3:50 PM

iPSC-derived Fibroblasts Timothy J. Kamp MD, PhD Professor of Medicine Co-director, Stem Cell and Regenerative Medicine Center Cellular and Molecular Arrhythmia Research Program University of Wisconsin-Madison School of Medicine and Public Health

3:50 – 4:10 PM

Hippo signaling in heart regeneration James F. Martin MD PhD Professor Vivian L. Smith Chair in Regenerative Medicine Baylor College of Medicine

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4:10 – 4:30 PM

Isolation of chamber-specific cardiomyocytes from differentiating pluripotent stem cells Reza Ardehali, MD, PhD Associate Professor, Cardiology; Molecular, Cellular and Integrative Physiology University of California – Los Angeles (UCLA)

4:30 – 4:50 PM

Transcriptional Regulation in the Regenerating Heart Enzo Porrello, PhD Associate Professor, Cardiac Regeneration Laboratory, Murdoch Childrens Research Institute, The Royal Children's Hospital, Melbourne

4:50 – 5:10 PM

Cross-talk via Circulating Myocardial MicroRNAs Gangjian Qin, MD Professor of Medicine and Biomedical Engineering Department of Biomedical Engineering School of Medicine, School of Engineering UAB | The University of Alabama at Birmingham

5:10 – 5:30 PM

hPSCs for Cardiac Repair Huangtian Yang, MD, PhD Professor and Principal Investigator Laboratory of Molecular Cardiology Shanghai Institute of Nutrition and Health

Shanghai Institutes for Biological Sciences China Academy of Sciences Shanghai, China

_____________________________________________________________________________ 5:30 – 5:45 PM Coffee Break _____________________________________________________________________________

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Session XI: Meet the Editor 6:00 – 6:20 PM Chairs: Keiichi Fukuda / Wolfram Zimmermann 5:45 – 6:00 PM

Caitlin A. Czajka, PhD Associate Editor Science Translational Medicine

Concluding Remarks – 6:00 PM

Jay Zhang, MD, PhD Chair, Department of Biomedical Engineering T. Michael and Gillian Goodrich Endowed Chair of Engineering Leadership Professor of Medicine, of Engineering School of Medicine, School of Engineering UAB | The University of Alabama at Birmingham

Welcome to Kyoto CVBE2021, Japan

Keiichi Fukuda, MD, PhD Professor Department of Cardiology Keio University, Japan

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Symposium Planning Committee:

Wolfram Zimmermann, MD University Medical Center Göttingen, Germany.

Jay Zhang, MD, PhD UAB | The University of Alabama at Birmingham

Malte Tiburcy, MD University Medical Center Göttingen, Germany

Laura Zelarayan, PhD University Medical Center Göttingen, Germany

Susanne Lutz, PhD University Medical Center Göttingen, Germany

Norman Liaw, PhD University Medical Center Göttingen, Germany

Patapia Zafeiriou, PhD University Medical Center Göttingen, Germany

Rabea Hinkel, DVM German Primate Center Göttingen, Germany

Symposium Administrative Coordinators: Jutta Creydt (University Medical Center, Göttingen, Germany) Kelly M. Hillard (University of Alabama at Birmingham, US) Axel Kaul (German Center for Cardiovascular Research, Partner Site, Göttingen, Germany) Annette Kuhring (German Center for Cardiovascular Research, Partner Site, Göttingen, Germany) Vanessa Kruse (German Center for Cardiovascular Research, Partner Site, Göttingen, Germany) Locations:

https://www.dpz.eu/en/home.html Deutsches Primatenzentrum Kellnerweg 4 37077 Göttingen Paulinerkirche Papendiek 14 37073 Göttingen https://www.sub.uni-goettingen.de/en/about-us/portrait/history/paulinerkirche/