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These reports are an opportunity for These reports are an opportunity for you to explain what you did and what you to explain what you did and what you found so that someone else can you found so that someone else can understand it.understand it.
Practice writing.Practice writing. Need a full explanation Need a full explanation a complete a complete
“story”.“story”. Need clear, direct writing.Need clear, direct writing. Need writing that makes sense and Need writing that makes sense and
leads the reader along a logical path.leads the reader along a logical path.
PRACTICE WRITINGPRACTICE WRITING
Some common problems:Some common problems:
1. Don’t over use words such as:1. Don’t over use words such as:““Then”Then” “Next” “After” “Again” “Next” “After” “Again”
2. Don’t use two verbs when one will do.2. Don’t use two verbs when one will do.Don’t “add” and “mix”Don’t “add” and “mix”Don’t “take” and “weigh” or “take” and Don’t “take” and “weigh” or “take” and “place”“place”Don’t “place” and “spin”Don’t “place” and “spin”
3. Don’t “weigh out” or “decant off” or 3. Don’t “weigh out” or “decant off” or “precipitate out”.“precipitate out”.
4. Some things are understood:4. Some things are understood:You will use clean tubes.You will use clean tubes.You will label your tubes properly.You will label your tubes properly.
Miscellaneous PointsMiscellaneous Points
1. The words “1. The words “thenthen” and “” and “thanthan” are not the ” are not the same.same.
You come to class, You come to class, thenthen you fall asleep. you fall asleep.
Everyone likes Sternfeld better Everyone likes Sternfeld better thanthan Rossi.Rossi.
2. The word 2. The word AbsorbancyAbsorbancy is not used. is not used.
3. No “centrifuge 3. No “centrifuge machinemachine” or ” or “spectrophotometer “spectrophotometer machinemachine”.”.
4. Be careful in using “4. Be careful in using “aa” versus “” versus “thethe”.”.We made an homogenate. We pipetted 2 ml of the homogenate into a We made an homogenate. We pipetted 2 ml of the homogenate into a
centrifuge tube. The centrifuge tube was spun in a centrifuge.centrifuge tube. The centrifuge tube was spun in a centrifuge.
Which of the following need fixing?Which of the following need fixing?
Tubes were added to a boiling beaker of Tubes were added to a boiling beaker of water.water.
The tubes were then placed in a The tubes were then placed in a centrifuge and run for 2 min.centrifuge and run for 2 min.
120 ml of distilled water was put in a 120 ml of distilled water was put in a blender and turned on.blender and turned on.
The pellet was resuspended with 5 ml of The pellet was resuspended with 5 ml of 5% TCA and centrifuged again for two 5% TCA and centrifuged again for two minutes and combined with the other minutes and combined with the other test tube.test tube.
The tube was placed in the centrifuge and spun for 2 minutes. After 2 minutes the supernatant was then discarded. After discarding the supernatant 5 ml of 10% TCA was then added and mixed. The tube was then put into the centrifuge and spun for 2 minutes. When the 2 minutes was up the tube was removed from the centrifuge and the supernatant removed with a Pasteur pipette and discarded into a waste container. Then the 95% ethanol was added with the graduated cylinder to the pellet. It was mixed and put into the centrifuge and spun for 2 minutes.
The tube was placed in the centrifuge and spun for 2 minutes. After 2 minutes the supernatant was then discarded. After discarding the supernatant 5 ml of 10% TCA was then added and mixed. The tube was then put into the centrifuge and spun for 2 minutes. When the 2 minutes was up the tube was removed from the centrifuge and the supernatant removed with a Pasteur pipette and discarded into a waste container. Then the 95% ethanol was added with the graduated cylinder to the pellet. It was mixed and put into the centrifuge and spun for 2 minutes.
The tube was spun in the centrifuge for 2 min.
The tube was placed in the centrifuge and spun for 2 minutes. After 2 minutes the supernatant was then discarded. After discarding the supernatant 5 ml of 10% TCA was then added and mixed. The tube was then put into the centrifuge and spun for 2 minutes. When the 2 minutes was up the tube was removed from the centrifuge and the supernatant removed with a Pasteur pipette and discarded into a waste container. Then the 95% ethanol was added with the graduated cylinder to the pellet. It was mixed and put into the centrifuge and spun for 2 minutes.
After discarding the supernatant, 5 ml of 10% TCA was mixed with the pellet.
The tube was spun in the centrifuge for 2 min.
The tube was placed in the centrifuge and spun for 2 minutes. After 2 minutes the supernatant was then discarded. After discarding the supernatant 5 ml of 10% TCA was then added and mixed. The tube was then put into the centrifuge and spun for 2 minutes. When the 2 minutes was up the tube was removed from the centrifuge and the supernatant removed with a Pasteur pipette and discarded into a waste container. Then the 95% ethanol was added with the graduated cylinder to the pellet. It was mixed and put into the centrifuge and spun for 2 minutes.
The tube was again spun for 2 min and the resulting supernatant discarded.
The tube was spun in the centrifuge for 2 min. After discarding the supernatant, 5 ml of 10% TCA was mixed with the pellet.
The tube was placed in the centrifuge and spun for 2 minutes. After 2 minutes the supernatant was then discarded. After discarding the supernatant 5 ml of 10% TCA was then added and mixed. The tube was then put into the centrifuge and spun for 2 minutes. When the 2 minutes was up the tube was removed from the centrifuge and the supernatant removed with a Pasteur pipette and discarded into a waste container. Then the 95% ethanol was added with the graduated cylinder to the pellet. It was mixed and put into the centrifuge and spun for 2 minutes.
Ten ml of 95% ethanol was mixed with the pellet before spinning the suspension for 2 min.
The tube was spun in the centrifuge for 2 min. After discarding the supernatant, 5 ml of 10% TCA was mixed with the pellet. The tube was again spun for 2 min and the resulting supernatant discarded.
The tube was placed in the centrifuge and spun for 2 minutes. After 2 minutes the supernatant was then discarded. After discarding the supernatant 5 ml of 10% TCA was then added and mixed. The tube was then put into the centrifuge and spun for 2 minutes. When the 2 minutes was up the tube was removed from the centrifuge and the supernatant removed with a Pasteur pipette and discarded into a waste container. Then the 95% ethanol was added with the graduated cylinder to the pellet. It was mixed and put into the centrifuge and spun for 2 minutes.
100 words
The tube was spun in the centrifuge for 2 min. After discarding the supernatant, 5 ml of 10% TCA was mixed with the pellet. The tube was again spun for 2 min and the resulting supernatant discarded. 10 ml of 95% ethanol was mixed with the pellet before spinning the suspension for 2 min.
53 words
I never know what I think about something until I read what I’ve
written.
–William Faulkner
Concentration of DNA in the Liver