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The V.P. C.D.R. (Vibrating Probe). Presented by Zac Eichmeyer And BEMsS (BioElectromagnetic student Society). What's a Vibrating Probe (V.P.)?. The V.P. is an instrument capable of measuring current densities in a biological medium Capable of measuring 10 nA/cm 2 - PowerPoint PPT Presentation
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Presented by Zac EichmeyerAnd BEMsS (BioElectromagnetic student Society)
The V.P. is an instrument capable of measuring current densities in a biological mediumCapable of measuring 10 nA/cm2
It was developed in the 70’s to measure current pulses from an Oocyte
Non-Destructive measurementsCan measure activities in live cells
Offers an order of magnitude increase from current detection methods, and is more versatile than dyes
How does the V.P. Work? The V.P. works by vibrating a high
impedance probe horizontally ~10m The vibration is controlled by a
Piezoelectric Actuator The Probe is connected to a
Preamplifier which conditions the signal for the Lock-In Amplifier
The Lock-in amplifier locks the probes measurement to that of its vibration so to only read current density at its max displacement Reducing Noise, and giving a
voltage reading at the max of its amplitude
The data is then collected via a data logger and fed into a computer for analyzing
The V.P. Requirements:The probe
High impedence, greater than 5 MLow length to mass ratio
Piezoelectric Actuator (the bender)Operate between ± 45VBend at least 30m
The Lock-in AmplifierLock on to a 150 Hz – 1kHz signal
The Pre-AmpLow Noise
The DAQCapable of sampling faster than the vibration
frequency<1 kHz max
The OscillatorCapable of driving a Piezoelectric Actuator at a
frequency less then 1kHzThe Mechanical Manipulator
Connects the probe to the microscopeRigidHave 1 degree of motion (up and down) Thermally and electrically neutral
To be able to measure current densities <100 nA/cm2
To eliminate/control extraneous noiseTo build the appropriate test setup to
implement the probe with a microscope Implement into current BEMSS researchMeasure current densities in other biological
samplesTrack Ion concentrations as they relate to
Neutrophils
•The V.P. Overview(subsystems)
•Probes•Piezoelectric Actuator (Bender)•Pre Amp•Lock In Amplifier•Oscillator•DAQ•Housing •Mechanical Manipulator
•Project Overview (Top Down)