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The Ultimate Guide to Reversed Phase HPLC/UHPLC Selectivity Important! Only column selectivity parameters of the same class (i.e. Steric Interactions) may be compared between the columns featured here. Hydrophobic selectivity is the main mechanism of retention under reversed phase conditions. Within the column profiles the 5 different selectivity parameter classes are not on the same scale. O O PO47801116_W www.Phenomenex.com Trademarks Aeris and Synergi are trademarks and Clarity, Gemini, Luna, Lux, and Kinetex are registered trademarks of Phenomenex. Gemini and Kinetex EVO are patented by Phenomenex. U.S. Patent Nos. 7,563,367 and 8,658,038 and foreign counterparts. © 2016 Phenomenex, Inc. All rights reserved. Hydrocarbon Compounds Hydrophobicity High column hydrophobicity values indicate greater retention of carbon-containing analytes. Aq Aq USP: L1 Synergi ™ Hydro-RP Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High TMS TMS USP: L1 Luna ® C18(2) Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High TMS TMS USP: L87 Synergi Max-RP Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High USP: L1 TMS TMS Luna Omega C18 Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High TMS TMS USP: L1 Gemini ® C18 Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High TMS TMS Aq Aq USP: L1 Synergi Fusion-Rp Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High TMS TMS USP: L7 Luna C8(2) Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High USP: L1 TMS TMS Polar Polar Luna Omega Polar C18 Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High TMS TMS USP: L1 Gemini NX-C18 Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High USP: L1 TMS TMS + + Luna Omega PS C18 Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High TMS TMS USP: L11 Luna Phenyl-Hexyl Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High USP: L1 TMS TMS Kinetex EVO C18 Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High USP: L1 TMS TMS Kinetex C18 Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High XB- USP: L1 Si TMS TMS Kinetex XB-C18 Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High TMS TMS USP: L11 Gemini C6-Phenyl Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High TMS TMS USP: L43 F F F F F Luna PFP(2) Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High Aq Aq USP: L11 Synergi Polar-RP Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High USP: L7 TMS TMS Kinetex C8 Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High USP: L11 TMS TMS Kinetex Phenyl-Hexyl Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High USP: L11 TMS TMS Kinetex Biphenyl Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High USP: L43 F F F F F TMS TMS Kinetex F5 Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High TMS TMS USP: L10 N C Luna CN Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High Which solid support is right for your analysis? Performance Gains on ANY HPLC or UHPLC System Extensive Selection for Increased Retention Core-Shell Particles • Ultra-high efficiency at decreased backpressure (2.6 µm) • 3 µm or better efficiencies at 5 µm pressures (5 µm) • Easy method transfer between HPLC and UHPLC systems • Highest efficiencies on UHPLC systems (1.3 µm and 1.7 µm) Fully Porous Particles • Increased loadability due to higher surface area • Excellent mechanical strength • Large range of scalable particle sizes and selectivities Additional Selection Tips Polar Acids • Luna Omega PS C18 Alkaline Mobile Phases (pH 8-12) • Kinetex EVO C18 • Gemini NX-C18 • Gemini C18 • Gemini C6-Phenyl Aromatic Compounds • Kinetex F5 • Kinetex Biphenyl • Luna PFP(2) Synthetic Oligonucleotides • Clarity ® Oligo-XT • Clarity Oligo-RP Proteins (>10 kDa) • Aeris ™ WIDEPORE XB-C18 • Aeris WIDEPORE XB-C8 • Aeris WIDEPORE C4 Peptides (≤10 kDa) • Aeris PEPTIDE XB-C18 • Luna Omega PS C18 • Kinetex EVO C18 Chiral Compounds • Lux ® Amylose-1 • Lux Cellulose-1 • Lux Cellulose-4 • Lux i-Cellulose-5 O O O Steric Interactions High column steric interaction values are best suited for the analysis of analytes that require separation based on size and shape differences. Identify Differences in Shape Selectivity TMS TMS USP: L7 Luna C8(2) Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High TMS TMS USP: L87 Synergi Max-RP Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High Positional Isomers - Polar/Neutral Functionalities USP: L43 F F F F F TMS TMS Kinetex F5 Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High TMS TMS USP: L43 F F F F F Luna PFP(2) Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High Isomers, Isobaric Compounds, and Shape Selectivity O O B + O – Polar Basic Compounds Cation Selectivity High column cation selectivity values will show higher retention for ionized bases. Low column cation selectivity values will have less interaction and retention for ionized bases, but may have very good peak shape. Increased Retention of Polar Bases USP: L11 TMS TMS Kinetex Biphenyl Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High USP: L43 F F F F F TMS TMS Kinetex F5 Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High USP: L1 TMS TMS Polar Polar Luna Omega Polar C18 Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High Improved Peak Shape for Bases USP: L1 TMS TMS + + Luna Omega PS C18 Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High USP: L1 TMS TMS Kinetex EVO C18 Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High Hydrogen Bond Donating Capacity Hydrogen bond donating groups on the silica surface interact with accessible functionalities containing a lone pair of electrons. Aq Aq USP: L1 Synergi Hydro-RP Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High TMS TMS USP: L1 Gemini C18 Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High TMS TMS USP: L87 Synergi Max-RP Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High TMS TMS USP: L1 Luna C18(2) Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High Non-ionized Bases and Oxygen- or Halogen-containing Compounds O O O H B ∙∙ Hydrogen Bond Accepting Capacity Hydrogen bond accepting groups on the silica surface interact with hydrogen bond donating functionalities on analytes. USP: L11 TMS TMS Kinetex Biphenyl Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High XB- USP: L1 Si TMS TMS Kinetex XB-C18 Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High USP: L1 TMS TMS Polar Polar Luna Omega Polar C18 Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High TMS TMS USP: L1 Gemini ® C18 Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High TMS TMS Aq Aq USP: L1 Synergi Fusion-RP Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High USP: L1 TMS TMS + + Luna Omega PS C18 Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High TMS TMS USP: L1 Luna C18(2) Hydrophobicity Steric Interaction Hydrogen Bond Donating Capacity Hydrogen Bond Accepting Capacity Cation Selectivity at pH 2.8 Cation Selectivity at pH 7.0 Low High Hydroxyl- or Amine-containing Functionalities O O ∙∙ X OH

The Ultimate Guide to Reversed Phase...The Ultimate Guide to Reversed Phase HPLC/UHPLC Selectivity Important! Only column selectivity parameters of the same class (i.e. Steric Interactions)

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Page 1: The Ultimate Guide to Reversed Phase...The Ultimate Guide to Reversed Phase HPLC/UHPLC Selectivity Important! Only column selectivity parameters of the same class (i.e. Steric Interactions)

The Ultimate Guide to Reversed Phase HPLC/UHPLC Selectivity

Important!

Only column selectivity parameters of the same class (i.e. Steric Interactions) may be compared between the columns featured here.

Hydrophobic selectivity is the main mechanism of retention under reversed phase conditions. Within the column profiles the 5 different selectivity

parameter classes are not on the same scale.

O O

PO

4780

1116

_W

www.Phenomenex.com

Trademarks Aeris and Synergi are trademarks and Clarity, Gemini, Luna, Lux, and Kinetex are registered trademarks of Phenomenex.Gemini and Kinetex EVO are patented by Phenomenex. U.S. Patent Nos. 7,563,367 and 8,658,038 and foreign counterparts.© 2016 Phenomenex, Inc. All rights reserved.

Hydrocarbon Compounds

HydrophobicityHigh column hydrophobicity values indicate greater retention

of carbon-containing analytes.

HILIC

Aq Aq

USP: L1

Synergi™ Hydro-RPHydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

TMSTMS

USP: L1

Luna® C18(2)Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

HILIC

TMSTMS

USP: L87

Synergi Max-RPHydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

USP: L1

TMSTMS

Luna Omega C18Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

HILIC

TMSTMS

USP: L1

Gemini® C18Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

HILIC

TMSTMS

Aq Aq

USP: L1

Synergi Fusion-RpHydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

TMSTMS

USP: L7

Luna C8(2)Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

USP: L1

TMSTMS

PolarPolar

Luna Omega Polar C18Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

HILIC

TMSTMS

USP: L1

Gemini NX-C18Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

USP: L1

TMSTMS

++

Luna Omega PS C18Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

TMSTMS

USP: L11

Luna Phenyl-HexylHydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

EVOC18

USP: L1

TMSTMS

Kinetex EVO C18Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

C18USP: L1

TMSTMS

Kinetex C18Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

XB-C18

USP: L1

Si TMSTMS

Kinetex XB-C18Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

HILIC

TMSTMS

USP: L11

Gemini C6-PhenylHydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

TMSTMS

USP: L43

F

F

F

F

F

Luna PFP(2)Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

HILIC

Aq Aq

USP: L11

Synergi Polar-RPHydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

C8USP: L7

TMSTMS

Kinetex C8Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

Phenyl-Hexyl

USP: L11

TMSTMS

Kinetex Phenyl-HexylHydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

Biphenyl

USP: L11

TMSTMS

Kinetex BiphenylHydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

F5USP: L43

F

F

F

F

F

TMSTMS

Kinetex F5Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

TMSTMS

USP: L10

N

C

Luna CNHydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

Which solid support is right for your analysis?

Performance Gains on ANY HPLC or UHPLC System Extensive Selection for Increased Retention

Core-Shell Particles• Ultra-high efficiency at decreased backpressure (2.6 µm)• 3 µm or better efficiencies at 5 µm pressures (5 µm)• Easy method transfer between HPLC and UHPLC systems• Highest efficiencies on UHPLC systems (1.3 µm and 1.7 µm)

Fully Porous Particles• Increased loadability due to higher surface area• Excellent mechanical strength• Large range of scalable particle sizes and selectivities

Additional Selection Tips Polar Acids• Luna Omega PS C18

Alkaline Mobile Phases (pH 8-12)• Kinetex EVO C18• Gemini NX-C18• Gemini C18• Gemini C6-Phenyl

Aromatic Compounds• Kinetex F5• Kinetex Biphenyl• Luna PFP(2)

Synthetic Oligonucleotides• Clarity® Oligo-XT• Clarity Oligo-RP

Proteins (>10 kDa)• Aeris™ WIDEPORE

XB-C18• Aeris WIDEPORE

XB-C8• Aeris WIDEPORE C4

Peptides (≤10 kDa)• Aeris PEPTIDE

XB-C18• Luna Omega PS C18• Kinetex EVO C18

Chiral Compounds• Lux® Amylose-1• Lux Cellulose-1• Lux Cellulose-4• Lux i-Cellulose-5

O OO

Steric Interactions High column steric interaction values are best suited for the analysis of analytes that require separation based on size and shape differences.

Identify Differences in Shape Selectivity

TMSTMS

USP: L7

Luna C8(2)Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

HILIC

TMSTMS

USP: L87

Synergi Max-RPHydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

Positional Isomers - Polar/Neutral Functionalities

F5USP: L43

F

F

F

F

F

TMSTMS

Kinetex F5Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

TMSTMS

USP: L43

F

F

F

F

F

Luna PFP(2)Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

Isomers, Isobaric Compounds, and Shape Selectivity

O O

B+

O–

Polar Basic Compounds

Cation Selectivity High column cation selectivity values will show

higher retention for ionized bases.

Low column cation selectivity values will have less interaction and retention for ionized bases, but may have very good peak shape.

Increased Retention of Polar Bases

Biphenyl

USP: L11

TMSTMS

Kinetex BiphenylHydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

F5USP: L43

F

F

F

F

F

TMSTMS

Kinetex F5Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

USP: L1

TMSTMS

PolarPolar

Luna Omega Polar C18Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

Improved Peak Shape for Bases

USP: L1

TMSTMS

++

Luna Omega PS C18Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

EVOC18

USP: L1

TMSTMS

Kinetex EVO C18Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

Hydrogen Bond Donating Capacity

Hydrogen bond donating groups on the silica surface interact with accessible functionalities containing a lone pair of electrons.

HILIC

Aq Aq

USP: L1

Synergi Hydro-RPHydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

HILIC

TMSTMS

USP: L1

Gemini C18Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

HILIC

TMSTMS

USP: L87

Synergi Max-RPHydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

TMSTMS

USP: L1

Luna C18(2)Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

Non-ionized Bases and Oxygen- or Halogen-containing Compounds

O OO

H

B∙∙

Hydrogen Bond Accepting Capacity Hydrogen bond accepting groups on the silica surface interact

with hydrogen bond donating functionalities on analytes.

Biphenyl

USP: L11

TMSTMS

Kinetex BiphenylHydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

XB-C18

USP: L1

Si TMSTMS

Kinetex XB-C18Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

USP: L1

TMSTMS

PolarPolar

Luna Omega Polar C18Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

HILIC

TMSTMS

USP: L1

Gemini® C18Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

HILIC

TMSTMS

Aq Aq

USP: L1

Synergi Fusion-RPHydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

USP: L1

TMSTMS

++

Luna Omega PS C18Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

TMSTMS

USP: L1

Luna C18(2)Hydrophobicity

Steric InteractionHydrogen Bond Donating Capacity

Hydrogen Bond Accepting CapacityCation Selectivity at pH 2.8Cation Selectivity at pH 7.0

Low High

Hydroxyl- or Amine-containing Functionalities

O O∙∙X

OH

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Low Voltage Selectivity QT1_EN

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Reversed carnot cycle

Reversed carnot cycle

Engineering
1 Selectivity Estimation

1 Selectivity Estimation

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Reaction Selectivity

Reaction Selectivity

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The Ultimate Guide to HPLC/UHPLC · PDF fileThe Ultimate Guide to HPLC/UHPLC Reversed Phase Selectivity ... Contained within the following pages is an easy overview of the reversed

The Ultimate Guide to HPLC/UHPLC · PDF fileThe Ultimate Guide to HPLC/UHPLC Reversed Phase Selectivity ... Contained within the following pages is an easy overview of the reversed

Documents
Selectivity and Backup

Selectivity and Backup

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SELECTIVITY and CROSS-REACTIVITY

SELECTIVITY and CROSS-REACTIVITY

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A retrospective investigation of selectivity for Pacific halibut CAPAM Selectivity workshop

A retrospective investigation of selectivity for Pacific halibut CAPAM Selectivity workshop

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Protection Coordination and Selectivity

Protection Coordination and Selectivity

Documents
Excellent selectivity, highest recovey

Excellent selectivity, highest recovey

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The Ultimate Guide to HPLC/UHPLCphx.phenomenex.com/lib/BR83791210_I.pdf · most appropriate reversed phase HPLC or UHPLC column. Though the chart below depicts several similar bonded

The Ultimate Guide to HPLC/UHPLCphx.phenomenex.com/lib/BR83791210_I.pdf · most appropriate reversed phase HPLC or UHPLC column. Though the chart below depicts several similar bonded

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reVersed Normals

reVersed Normals

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Media Use and Selectivity

Media Use and Selectivity

Documents
Robin Hood Reversed

Robin Hood Reversed

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