The Skin MicrobiomeFor a personalised beauty care

PERSONALCARE &COSMETICS

This integrated approach combines 3 elements:

Step 1 The selection of the right panel. The right panel of volunteers is screened and selected according to skin bacterial communities.

Step 2 The proof of product effect on the selected panel of volunteers through an integrated and multi-tool approach MALDI-TOF based microorganism identification with the addition of clinical test evaluation with visual evidence.

Step 3 Microbiome study through metabarcoding. The study of the taxonomical metabarcoding provides the composition of skin flora.

Our experts dedicated

to skin microbiota

projects are available to design with

you a specific protocol that

fits your needs

Step 1 • the selection of the right panelThe right panel of volunteers is screened and selected according to skin bacterial communities.Screening results to select the right panel for specific efficacy test services are based on the total bacterial count through a direct collection of skin microbiota on volunteers.

Step 2 • test your product effect on a selected panelThanks to a customizable evaluation on skin microbiota before and after product application, we can prove the product effect on selected volunteers with an integrated and multi-tool approach: new microbiological method associated with MALDI-TOF identification.

Our integrated & multi-tool approach exploits the synergies of different techniques:• Quantification associated to rapid MALDI-TOF identification that represent a new microbiological

approach to investigate how a product works on skin microbiota.• Clinical test & visual evidence: the clinical proof of your product effect on the selected panel.

How does your product work on Skin Microbiota?

The clinical proof of your product efficacy

CLINICAL TEST EVALUATION & VISUAL EVIDENCE

MALDI-TOF BASED MICROORGANISM IDENTIFICATION

Our studies on dry and sensitive skin

How does a product work on skin microbiota?Volunteers responded well to the treatment with a specific cream intended for dry and sensitive skin or for skin with tendency to atopic dermatitis.

RESULTSkin microbiota increased after the treatment

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Our studies on oily skinHow does a product work on skin microbiota?Volunteers with oily skin reduced the high levels of microbiota upon product application. Indeed, in 80 % of volunteers the levels of microbiota are shifted towards normal-microbiota levels.

RESULTThe treatment balanced the skin microbiota.

THE FACT

Acne-prone skin and oily skin tend to develop comedones and pimples, and often appears shiny.

THE FACT

Dry and sensitive skin is one of the condition that could alter the balance of microbiota and make the skin more exposed to external attacks. The hydration level in the surface layer is an important factor affecting the biophysical properties and function of the skin barrier.

Our studies on axilla skinHow does a product work on axilla microbiota?Volunteers showing a flora dominated by Corynebacteria are characterized by a more intense axillary odour and this result was confirmed by the traditional sniff test.

THE FACT

Sweating is both healthy and natural and odour perspiration is actually odourless, but when bacteria come into contact with sweat dripping from the pore, they cause body odour.

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RESULTDeodorants containing antimicrobial agents have a direct effect on axilla microbiota and therefore they are more efficient in controlling/reducing sweat odour.

Our studies on body skinHow does a product work on skin microbiota?Volunteers continued to show a normal microflora after product applications: the most abundant microorganisms identified are human skin normal commensals and no evident dysbiosis was detected.

THE FACT

Despite the good balance, normal skins are unable to cope with all the aggressions encountered in a lifetime, such as surfactants, detergents, cosmetic products, sunscreen, scrubs or repeated friction of tissues on the skin, all this can damage the protective film.

RESULTSkin microbiota has been preserved in a short period of product application

Step 3 • taxonomical metabarcoding studyStudy of the composition of skin flora: taxonomical metasequencing

Skin microbiome analysis: points of attention

• Study design with well-defined endpoints• Skin flora monitoring• Bioinformatic analysis by an expert

LEVEL 1only descriptive statistics provided

• Taxonomic profiles of each sample• Calculation of diversity and richness indexes• Report including tables and graphs (barplots,

heatmaps, krona, hierarchical clustering, PCoA)

LEVEL 2custom inferential statistical analysis

• Detection of differentially abundant features between conditions

• Investigation of the relationships between clinical or environmental variables and taxonomic compositions

LEVEL 3ad hoc solutions

• Scientific interpretation with reviewing of literature

• Next strategy proposal• Scientific communication

Skin MicrobiomeHuman skin is a complex ecosystem with various micro-environmental conditions and microbial communities, and it is the home to millions of bacteria, fungi and viruses which compose its microbiota. It is extremely important to the skin health and it plays an essential role in protecting it against external infectious or toxic substances and allergens.

Skin microbial communities are site-, individual-, and race-specific, and they are largely stable over time, despite skin’s exposure to different external environments. Various factors, including the use of antibiotics, cosmetics, soaps, personal care products, and living conditions such as life styles and nutrition habits can influence the skin microbiome.

Various factors can disrupt the balance of microbiota and cause skin dysbiosis.

Described as an imbalance between the microbiota and its host, dysbiosis can be considered a form of impaired homeostasis in which the microbiota is shifted towards a less complex, less varied pathological spectrum.

Researchers have suggested that the fragile balance of the skin microbiota may have a strong influence on the functional differences between healthy skin and diseased or damaged skin, and that strong treatments can deeply alter the microbiota and compromise the health of the skin.

It is important to maintain the

microbiota equilibriumto preserve

the best skin physiology.

Mérieux NutriSciences has developed a sound strategy to provide a complete service to prove the real effect of beauty products that claim for microbiota or microbiome.

Via Fratta 25, 31023 Resana (TV) - Phone +39 0423 7177E-mail: cosmetics.italy@mxns.com

www.merieuxnutrisciences.com/eu

BR0518_0_ENG/Photos Shutterstock, Mérieux NutriSciences. Printed in ItalyCopyright©2019 Mérieux NutriSciences Corp. All Rights ReservedAll Rights Reserved

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focus onMicrobiota evaluation on skin dermatitis

Our studies suggest that a link between a varied skin microbiota and the course of dermatitis disease exists since its flares are associated with an expansion of Staphylococcus aureus on lesional skin and a substantial loss of biodiversity in microbiota.

Microbiota evaluation on thin fragile skin

Skin rash, dryness and peeling, swelling or facial redness, and itching are the main side effects that might occur after skin disorder, medications and in older adults.