The research aimed to utilize the The research aimed to utilize the anthocyaninanthocyanin components of the components of the MalunggayMalunggay. The researchers wanted to look for an alternative source . The researchers wanted to look for an alternative source of of anthocyaninanthocyanin that is of good quality and is sufficient to absorb and that is of good quality and is sufficient to absorb and thus eliminate fat from the body. thus eliminate fat from the body.

Moringa oleifera lamk Moringa oleifera lamk will yield a significant amount of will yield a significant amount of anthocyanin anthocyanin that when utilized, will be an effective fat-eliminator in terms of the that when utilized, will be an effective fat-eliminator in terms of the different parameters such as Fat-eliminators must be able to bind and different parameters such as Fat-eliminators must be able to bind and eliminate fat in the body, The fat-eliminator must have no adverse eliminate fat in the body, The fat-eliminator must have no adverse effects on the body, The fat-eliminator must focus only on the fat cells effects on the body, The fat-eliminator must focus only on the fat cells and must not cause deterioration of other bodily tissues such as and must not cause deterioration of other bodily tissues such as muscles. muscles.

1. Specifically, this study will attempt to answer the following 1. Specifically, this study will attempt to answer the following questions:questions:What will be the best method to isolate What will be the best method to isolate anthocyaninanthocyanin from from Malunggay?Malunggay?

1.1 Using Solvent Extraction Systems1.1 Using Solvent Extraction Systems1.2 Column Chromatography Process1.2 Column Chromatography Process

2. Will Malunggay yield a significant amount of good quality 2. Will Malunggay yield a significant amount of good quality anthocyaninanthocyanin which can be utilized as a fat-eliminator? which can be utilized as a fat-eliminator?

3. How effective is the extracted 3. How effective is the extracted anthocyaninanthocyanin from Malunggay from Malunggay as fat-eliminator?as fat-eliminator?

3.1 Anthocyanin extracted must be able to dissolve and 3.1 Anthocyanin extracted must be able to dissolve and eliminate fat.eliminate fat.3.2Anthocyanin extracted must be able to dissolve and 3.2Anthocyanin extracted must be able to dissolve and eliminate a significant amount of fat.eliminate a significant amount of fat.3.3 Anthocyanin extracted must be able to eliminate fat only.3.3 Anthocyanin extracted must be able to eliminate fat only.

The study aimed to investigate the action of the The study aimed to investigate the action of the anthocyanin anthocyanin extracted extracted from from Moringa oleifera lamk. Moringa oleifera lamk. It also aimed to discover which method of It also aimed to discover which method of extraction would yield a significant amount of anthocyanin since the extraction would yield a significant amount of anthocyanin since the extraction is a crucial part of the whole experimentation. It was extraction is a crucial part of the whole experimentation. It was performed within Metro Manila in the year 2009. This research started performed within Metro Manila in the year 2009. This research started on the second week of June 2009 and ended on October 20, 2009. The on the second week of June 2009 and ended on October 20, 2009. The proposed study was all about the isolation of Anthocyanin from proposed study was all about the isolation of Anthocyanin from Malunggay Malunggay and its utilization as a fat-eliminator. As contribution to the and its utilization as a fat-eliminator. As contribution to the efforts to stand up from recession, the researchers used readily efforts to stand up from recession, the researchers used readily available, efficient but cost-effective materialsavailable, efficient but cost-effective materials

The study aimed to utilize The study aimed to utilize anthocyanin anthocyanin in the Malunggay plant. Also, in the Malunggay plant. Also, this aimed to find the method of isolating anthocyanin which will have this aimed to find the method of isolating anthocyanin which will have the greatest yield and best quality of the antioxidant. The researchers the greatest yield and best quality of the antioxidant. The researchers thought that the study would be vital to the field of science, especially thought that the study would be vital to the field of science, especially in the medical field, for it can be a solution to the ever-increasing in the medical field, for it can be a solution to the ever-increasing problem of obesityproblem of obesity

Problem Conceptualization

Formulation of Hypothesis

Preliminary Investigation

Formulation of Experimental Design

Gathering of Equipment/Materials needed

Experiment Proper

1st stage of experimentation

Solvent Extraction Systems

Thin Layer and Column Chromatography

Anthocyanin from Setup X

Determining the setup which yields the most amount of Anthocyanin

2nd stage of experimentation

Oil Dispersion Test

Analysis of Data Obtained

Conclusions and Recommendations

SAMPLESAMPLEDPPH (DPPH (íímol TE/g Sample, DM Basis)mol TE/g Sample, DM Basis)

DichloromethaneDichloromethane 15.6915.69

AcetoneAcetone 19.2319.23

MethanolMethanol 42.7742.77

Hexane (Flash Chromatography)Hexane (Flash Chromatography) 29.1229.12Table 1 – Antioxidant Activity of Moringa Oleifera Lamk using DPPH test

Table 1 shows the different values of DPPH in the extracts. The higher the value Table 1 shows the different values of DPPH in the extracts. The higher the value represented by the DPPH, the greater the concentration of represented by the DPPH, the greater the concentration of anthocyaninanthocyanin in the in the extract. The methanol extract shows the highest value of DPPH while extract. The methanol extract shows the highest value of DPPH while Dichloromethane shows the least.Dichloromethane shows the least.  

Amount of Water used Amount of Oil used Amount of Crude Extract used

300mL 50mL 50ml

Table 2 – Constant Quantities used in the experiment

Table 2 shows the quantities that were constant when the experiment was done. This Table 2 shows the quantities that were constant when the experiment was done. This assured accurate results from the experiment.assured accurate results from the experiment.

Diameter (in cm.) Time it took for the hole to close.

(In sec.)

Amount of oil that remained

(in ml.)

9.6 ~8 48.3

Table 3 - Data obtained from oil dispersion test using Methanol

Table 3 shows the data obtained from oil dispersion test using Methanol crude Table 3 shows the data obtained from oil dispersion test using Methanol crude extract. Quantities of diameter and time it took for the hole to close were data from extract. Quantities of diameter and time it took for the hole to close were data from 5 trials because the crude extract of 50ml was separated into five 10ml 5 trials because the crude extract of 50ml was separated into five 10ml applications and the quantities obtained were recorded and averaged. The amount applications and the quantities obtained were recorded and averaged. The amount of oil that remained was also measured to determine the fat-dissolving action of of oil that remained was also measured to determine the fat-dissolving action of anthocyanin.anthocyanin.

This research entitledThis research entitled “ “The Effectiveness of The Effectiveness of AnthocyaninAnthocyanin extracted from extracted from Moringa Moringa Oleifera LamkOleifera Lamk as a fat-eliminator” has been conceptualized out of pure curiosity: if as a fat-eliminator” has been conceptualized out of pure curiosity: if malunggay contained a significant amount of anthocyanin and if it would be effective malunggay contained a significant amount of anthocyanin and if it would be effective in the elimination of fat. The researchers also wanted to know which method of in the elimination of fat. The researchers also wanted to know which method of extraction would yield the most amount of anthocyanin. The researchers chose flash extraction would yield the most amount of anthocyanin. The researchers chose flash chromatography with hexanes used as its solvent and water-bath assisted solvent chromatography with hexanes used as its solvent and water-bath assisted solvent extraction methods using acetone, dichloromethane and methanol as their solvents as extraction methods using acetone, dichloromethane and methanol as their solvents as their methods of extraction. The researchers put in mind that the significant their methods of extraction. The researchers put in mind that the significant anthocyanin content would be able to bind and dissolve fat. anthocyanin content would be able to bind and dissolve fat. The researchers used the four extraction methods and they have proven that The researchers used the four extraction methods and they have proven that Moringa oleifera lamk Moringa oleifera lamk absolutely contained a significant amount of absolutely contained a significant amount of anthocyanin. anthocyanin. Through the four extraction methods, they found out and at the same time concluded Through the four extraction methods, they found out and at the same time concluded that methanol produced and showed the greatest capability in extracting phenolic that methanol produced and showed the greatest capability in extracting phenolic antioxidants and inhibiting the free radicals produced by DPPH among four solvents antioxidants and inhibiting the free radicals produced by DPPH among four solvents used. The extracts were dropped in water with oil, after doing so; there was a used. The extracts were dropped in water with oil, after doing so; there was a dispersion of the oil molecules indicating that there is dissolving or elimination of fat dispersion of the oil molecules indicating that there is dissolving or elimination of fat because the oil represents fat. Also, the original amount of oil was reduced after the because the oil represents fat. Also, the original amount of oil was reduced after the experiment, indicating that the experiment, indicating that the anthocyaninanthocyanin in the crude extract was able to make in the crude extract was able to make the oil molecules soluble in water. Two experiments using different crude extracts the oil molecules soluble in water. Two experiments using different crude extracts were done and the data was recorded and analyzed. Because of this, the researchers were done and the data was recorded and analyzed. Because of this, the researchers were able to confirm that were able to confirm that anthocyaninanthocyanin could be used as a fat-eliminator. could be used as a fat-eliminator.

The researchers conclude that The researchers conclude that malunggaymalunggay absolutely contains a significant amount absolutely contains a significant amount of of anthocyaninanthocyanin that is able to dissolve fat. Furthermore, the best method that that is able to dissolve fat. Furthermore, the best method that produced the most yields was through water-bath assisted solvent extraction produced the most yields was through water-bath assisted solvent extraction method using methanol as its solvent. The researchers also conclude that method using methanol as its solvent. The researchers also conclude that anthocyaninanthocyanin dissolves fat which was proved by the oil dispersion test where there dissolves fat which was proved by the oil dispersion test where there was dispersion in the oil column and reduction of the original volume of the oil used was dispersion in the oil column and reduction of the original volume of the oil used prior to experimentation, signifying that prior to experimentation, signifying that anthocyaninanthocyanin made the oil molecules soluble made the oil molecules soluble in water.in water.

 

The researchers recommend further study to be conducted regarding this topic. The researchers recommend further study to be conducted regarding this topic. Since the researchers only made use of two methods, further study may lead to Since the researchers only made use of two methods, further study may lead to discovery of ways on how to extract discovery of ways on how to extract anthocyaninanthocyanin from from malunggaymalunggay and also be able and also be able to yield the maximum amount of to yield the maximum amount of anthocyanin.anthocyanin. The researchers used The researchers used malunggaymalunggay because it is widely available in the country and it is economically and scientifically because it is widely available in the country and it is economically and scientifically proven to be safe and nutritious. Knowing this, the researchers recommend using a proven to be safe and nutritious. Knowing this, the researchers recommend using a different plant that may contain antioxidants that may also eliminate fat. In addition different plant that may contain antioxidants that may also eliminate fat. In addition to this recommendation, the researchers also recommend to further study the to this recommendation, the researchers also recommend to further study the properties of properties of anthocyaninanthocyanin, its fat-dissolving action and its utilization, especially on , its fat-dissolving action and its utilization, especially on making some kind of dietary supplement which is safe for humans, effective in making some kind of dietary supplement which is safe for humans, effective in terms of fat-dissolving and is affordable.terms of fat-dissolving and is affordable.