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The problem

How to integrate the massive amounts of data on Drosophila neurobiology to explore anatomy, formulate hypotheses and find reagents?

Behavior/neural process ontology on FlyBaseGene ontology annotations of genesneurological system process7,904 annotations coverring 3,099 proteins and 121 termsbehavior1,964 annotations coverring 932 proteins and 89 terms

PhenotypesNumber of annotations: 4961Number of terms used in annotation: 24 all defined using GO.Gene ontology annotations of anatomical structuresneurological system process: 1479detection of stimulus involved in sensory perception: 1467GO behavior: 17Genetic dissection of neural circuit functiontransgenic driver(s) target expression to specific neuron classes

repress

neural activityactivatemodulatebehaviordirect assays of neural activityUAS - < some activity modulator >

Mutantreplace this with optic system neuron

ExpressesFru

Scer\GAL4[fru-NP0021]

ExpressesDA1 vPNGO: response to pheromoneGO: sensory perception of smellGO: male courtship behaviorFunctionPhenotypePhenotypeAnatomical phenotypes

registration(warping)e.g.- neurons with some part in AMMC

QUERIEScomputation of overlap3D co-ordinates for neuron Neuron based bioinformatics: tracking 3D data

3D co-ordinates for Brain regions

segmentationStandard brain => standard 3D coordinate space Analysis of cross-registered 3 D image dataPredicted innervation patternsNeuronBlast => predictions of neuron classesPredicted connectivity from Arbor proximity

300 m / 40,000 neurons3D overlap defines potential connectivity

OutputIn the last year and half at the LMB, one project that has been going well is a mapping effort to better understand the neuronal circuit required for male courtship. These neurons, about 2000 out of 100,000 in the fly brain are labelled by the gene fruitless. We have found that Olfactory circuit -> studies on pheromone -> ORNs axons -> Projection neuron dendrites -> Axon/axon terminals -> Males females physiology is the same ->Difference must be deeper in brain7

300 m / 40,000 neurons3D overlap defines potential connectivity

In the last year and half at the LMB, one project that has been going well is a mapping effort to better understand the neuronal circuit required for male courtship. These neurons, about 2000 out of 100,000 in the fly brain are labelled by the gene fruitless. We have found that Olfactory circuit -> studies on pheromone -> ORNs axons -> Projection neuron dendrites -> Axon/axon terminals -> Males females physiology is the same ->Difference must be deeper in brain8QueriesNeurons by function (inferred from phenotype or electrophysiology)

Phenotypes due to modulation of neurons in expression domain of driver X

Potential circuit partners direct or indirect, with X functionFunctional annotation using GO

Functional annotation using GOWhat we needClear definitions and safe inferenceinfer classificationAvoid vertebrate specificityreferences to anatomical structuresreferences to species specific movement typesBundling together of functions in species specific ways (vestibulo-auditory)Cross ontology integration GO; Uberon; CHEBI, PATO