"The perspective of the MOEW in the management of tightening controls on pesticides in the framework of strategic plans of UAE" The third Topic Evaluation

"The perspective of the MOEW in the management of tightening controls on pesticides in the framework of strategic plans of UAE"

The third TopicEvaluation of Pesticides Quality & Monitoring of Pesticide residues in Fruits & Vegetables in UAE Markets.

04/19/23 1Pesticides Laboratories

04/19/23 Pesticides Laboratories 2

INTRODUCTIONScientific research over the last decades has shown that a diet rich in fruits and vegetables is protective against many cancers and lowers the occurrence of coronary heart disease. This recognition of the importance of routine consumption of fresh fruits and vegetables, together with a marked increase in the year round availability of fresh fruits and vegetables from a global market, has contributed to the substantial increase in consumption of fresh fruits and vegetables over the past two decades. However, the recent increase in reports of food borne illness associated with fresh fruits and vegetables has raised concerns from public health agencies and consumers about the safety of these products.


OBJECTIVES•To ensure the safety of food (fruits & vegetables ) as far as pesticides contamination is concerned• planning of surveillance programmes for pesticide residues inthe UAE food supply and the evaluation of the results;

•procedures for sampling, sample processing, new methods ofanalysis, the assessment of variability of pesticide residues infruits &vegetables and related issues.


Surveillance programme

The pesticide residues surveillance programme is designed to enable us to check:

•that specified pesticide maximum residue levels (MRLs ) are being respected & compiled with;

•that users of pesticides are complying with conditions of use specified inthe authorisation; and

•that dietary intakes of residues are within acceptable limits.

Pesticides

Ionic Compounds

Mycotoxins Veterinary Drugs

FoodBorne

Exposures

Contaminants


Infertility

Cancer

Brain Behavior

Food borne Illness

Mutation


• Ultimately, the quality and safety of food depends on the efforts of every one involved in the complex chain of agriculture production, processing, transport, food production and consumption. World Health Organization (WHO) views food safety as a shared responsibility from farm to fork.


http://www.fwi.co.uk/community/photos/matthewbrown/towingjpg-173783.aspx

http://photography.nationalgeographic.com/staticfiles/NGS/Shared/StaticFiles/Photography/Images/POD/w/wheat-farm-494831-sw.jpg

This monitoring study was conducted in the Central laboratories, Ministry of Environment and Water, Al Ain, which are accredited by UKAS according to ISO/IEC17025 :2005 for pesticide residues analysis in fruits and vegetables.



04/19/2310 Pesticides Laboratories

04/19/23Pesticides Laboratories 11

International International AccreditationAccreditation

PL is Accredited by UKAS (United Kingdom Accreditation Services) as per the requirements of (ISO/ IEC 17025) since 2004.PL is the 1st Laboratory in the middle east & GCC accredited in the field of Pesticides Formulations Analysis and the Analysis of Pesticides Residues in Fruits & Vegetables. (scope of accreditation covers around 200 Pesticides).In the PF there is a strong similarity in the method used in PFL and CIPAC methodology.


• PRL Staff are conducting monitoring surveys for most commodity types of imported fresh Fruits and Vegetables (mainly from Guaifat Entry point & Agricultural Quarantine Centres)

• The Annual Average Samples Number is more than 2500 & is in the increase

• PRL can test now around 300 pesticides in each sample (to be increased to around 400 pesticides later this year).


Fruits and Vegetables Samples



Methodology The ultimate goal in fair practice in international trade depends, among other things, on the reliability of analytical results. This in turn, particularly in pesticide residue analysis, depends not only on the availability of reliable analytical methods, but also on the experience of the analyst and on the maintenance of ‘good practice in the analysis of pesticides’. These include good analytical practice and may be considered in three inter-related parts:

The Analyst Basic Resources The Analysis

The requirements for facilities, management, personnel, quality assurance and quality control, documentation of results and raw data, and relevant subjects, which are considered as prerequisites for obtaining reliable and traceable results, are described in general in the ISO/IEC 17025 Standard (2005) and in a series of OECD, GLP Guidance Documents, in the corresponding national laws and regulations

Methods of Pesticide Residues Analysis

Multi-residues Analysis in Food Stuffs based on

modern GC techniques &

follows protocols as described in

SANCO04/19/2316 Pesticides Laboratories

Gas & Liquid Chromatography:•GC/PFPD•GC/MSD•GC/MSD+NPD•GC/MSD+NPD+ECD•GC/TSD+FID•GC/ECD+NPD•GC/FID•HPLC•LC/MS/Fluorescene•LC/MSMS

• Other equipments used for Homogenization, Extraction, Concentration , filtration and Clean-up of Sample Extracts.•Staff: 2-PhD, 1-MSc. and 4 BSc.(total 7)

17 04/19/23Pesticides Laboratories

Pesticides Laboratories

The use of QuEChERS (pronounced “Catchers”) method

– Quick, Easy, Cheap, Effective, Robust and Safe• Sample Preparation method developed jointly by US FDA and EU Food Regulatory Agencies• Methodology for simplified extraction of a large number of pesticide residues in fruits and

vegetables• QuEChERS is a fast sample preparation method for screening for analytes of interest, not for

concentrating samples • QuEChERS is still in it’s “Infancy”; being adopted worldwide

Page 18

Luke method or traditional SPEQuEChERSBottom Line

Estimated Time to process 6 samples (min)

120304x faster

Solvent Used (mL)90 mL10mL9 x less solvent

Chlorinated Waste (mL)30 mLnonesafer, greener, less costly

Glassware/ specialized equipment

Funnel, water bath, 200mL containers, evaporator, etc.

NoneNo additional supplies needed04/19/23 18


170 pesticidesGC/MSDLC/MS

Ethyl Acetate

400/500 pesticidesGC/MSD,

GC/MSMS, LC/MSMSQuEChERS


Routine-SpectrumSingle Pesticides

New MethodsUp to 1.000 Compounds!

Results:

The results of the analyses of 8245 samples is shown in the table below covering the period from 2005-2010. In this study about 28 types of fruits and 60 types of vegetables which mean 88 types of commodities from more than 30 countries from different parts of the world were analyzed for pesticide residues..


• The results shown were carried out using MRM method using LC-MS, GC-MS, GC-PFPD/TSD/NPD/µECD. In average more than 250 pesticides and their metabolites covering all the major classes organochlorines, organophosphorus and carbamates were tested for. For most of the pesticides the reporting limit was 0.01-0.02 mg/kg.



For our 2010 surveillance programme we are pleased to report that we have expanded the range of pesticides that we look for in our fruit and vegetables surveys up to 323 and have reduced the reporting limit in most cases to 0.01 mg/kg. In 2009 the maximum number of pesticides that we looked for in any survey was 136 with a routine reporting limit of 0.02mg/kg .

*For this reason we may in future find more residues and multi-residues in single samples.

Summary of Results (2005-2010)

CategoryNo. of Analyzed

SamplesSamples Free of

PR%Sample with PR

below MRL<

%Samples with PR Exceed >MRL%

Year

200570159084.57210.3395.7

200672053273.812617.5628.6

200760246477.18514.1528.6

200837829578.046416.8204.9

2009281321247630710.938213.6

20103031232776.738712.731810.4

Total8245633276.8104112.687310.6


Total S No

Year

7012005

7202006

8202007

3782008

28132009

30312010

1-There was an annual increase of analyzed samples using the luke method, from 701 in 2005 to 820 in 2007 reaching 3031 in 2010 with a percentage increase of 332 % compared to 2005.


2. There was also an increase in targeted pesticides from 76 in 2005 to 103 in 2007 & then to 323 pesticides in 2010 equivalent to a 325 % increase.

YearNo of pesticide

/sample

Percent Increase

20057620069018.4%200710314.4%200812925.2%20091365.4%2010323137.5%




Percentages of 2009 samples

14%

11%

75%

Sample exceedingMRL

Sample within MRL

Sample withoutdetectable residues

Percentages of 2010 samples

10%13%

77%

Sample exceedingMRL

Sample within MRL

Sample withoutdetectable residues

FOOD HANDLING TIPS

While pesticide residues on and in food are typically at very low levels, there are ways we can further reduce them:Rinse fruit and vegetables thoroughly under running water. Running water has an abrasive effect that soaking doesn't have. Also some pesticides are absorbed into fruits and vegetables, some are present on the surface. Washing removes most surface waxes and residues, along with dirt and bacterial contamination. Peeling fruits and vegetables also removes surface residues and some conjugated residues. (but remember that some nutrients and fiber may be lost in peeling)


Some residues are concentrated only in outer leaves so removal of outer leaves in cabbage, lettuce etc. can remove residues alsoCooking, canning, and other food processing methods can reduce some (not all) residues.Eating a variety of foods ensures balanced nutrient uptake; further it eliminates likelihood of exposure to a single residue and its bio-magnification(5-a day

Thank you

I would like to thankAll attendees for their attention
