Neuro-IT workshop – Bonn 22 June, 2004

The NEUROBIT projectThe NEUROBIT projectA bioartificial brain with an artificial body: training a cultured neural tissue to support the purposive

behavior of an artificial bodyIST - 2001- 33564 - 1 May 2002 – 30 April 2005

Sergio Martinoia (project coordinator)

Neuro-IT workshop – Bonn 22 June, 2004

Bi-directional in-vitro Neural interfaces• What is a bi-directional in-vitro neuronal interface?

in - vitro neuronal system “embodied” and “situated”

Bioartificial neuronal networks bioartificial living systems

• Bidirectional neuro-robotic interfaces used as model system for investigating adapitve properties and synaptic plasticity

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Neuro-IT workshop – Bonn 22 June, 2004

In-vitro neuronal networks and MEACortical cells are dissociated from rat embryos (E18) and kept in culture for

weeks (from 7 to 35 Days In Vitro – DIV).

Measurements are carried out in physiological medium: NaCl 150mM, CaCl2 1.3mM, MgCl2 0.7mM, KCl 2.8mM, Glucose 10mM, HEPES buffer 10mM.

Multichannel MEA - 60 TiN electrodes

Neurobit MEA - 60 Pt, Ir or IrOx electrodes

Neuro-IT workshop – Bonn 22 June, 2004

Voltage stimulations and long - term effects

Neuro-IT workshop – Bonn 22 June, 2004

Network stability and plasticity protocolsLEGEND

Test 1Test 2 post tetanus 1 Test 3 post tetanus 2

LEGENDTest 1Test 2Test 3Test 4

Neuro-IT workshop – Bonn 22 June, 2004

New developed MEA devices with cluster

Packaged device

Neuro-IT workshop – Bonn 22 June, 2004

Bi-directional connection and closed-loop experiments

As a closed-loop experiment, we focus on a simple ‘Braitenberg vehicle’ that (learns to) avoid obstacles. The robotic body is a Khepera II, with two wheels and eight infra-red (IR) proximity sensors, which moves inside a circular arena,containing a number of obstacles. Selectivity of population activity to the site of stimulation points to spatial coding of information. Therefore, we defined separate ‘motor’ and ‘sensory’areas. We used two separate sets of recording sites to control the left and right wheels of the robot

CODING

DECODING

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ROut

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LIn

Neuro-IT workshop – Bonn 22 June, 2004

Obstacle avoidance behavior

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Neuro-IT workshop – Bonn 22 June, 2004

PartnersPartners of the of the NeurobitNeurobit projectproject

Jaap van Pelt, Ildiko Vajda,Ger Ramakers, Arjen van Ooyen, Pieter Wolters

Netherlands Institute for Brain Research, KNAW, The Netherlands

KNAWKNAW

Fabrizio Davide, Federico Morabito, Giuliano Stillo, Emiliano Mazzone, Simone Cicero

Telecom Italia Learning Services S.p.A., TILS, ItalyTITI

Sylvie Renaud, Jean Tomas, LudovicAlvado, Thierry Orlandi

IXL Microelectronics Laboratory, ENSEIRB, CNRS, University of Bordeaux, France

IXLIXL

Gwendal Le Masson, André Garenne

The French Institute of Health and Medical Research, INSERM, France

INSERMINSERM

Milena Koudelka-Hep, Luca Berdondini

Institute of Microtechnology, University of Neuchatel, IMT, Switzerland

IMTIMT

Vittorio Sanguineti,Pietro Morasso, Laura Cozzi, Paolo D’Angelo

Department of Computer Science, Systems Engineering and Telematics, DIST, University of Genoa, Italy

UGDISTUGDIST

Sergio Martinoia, Michela Chiappalone,Brunella Tedesco, Laura Bonzano, Antonio Novellino, Alessandro Vato, Marco Bove

Department of Biophysical and Electronic Engineering, DIBE, University of Genoa, Italy

DIBEDIBE