The Model Experiment

A Complex Behavior

Modularize

Module A Module B Module C Module D

Find appropriate model system?

Literature

Candidate Genes

Experimental MethodsLab Assays

Yes No

Well studied non-model example

MutantsSearch

Study Evaluate

Analyze

Generate Hypotheses and design experiments

Literature

Candidate Genes

Experimental Methods

Behavior “orthologs”

Example Insect Behavior

Module A Module B Module CModule D

Fly, Mouse

Literature

Candidate GenesExperimental Methods Lab Assays

Termite

Mutants

Generate Hypotheses and design experiments

Literature

Candidate Genes

Experimental Methods

Fly

Fly, MouseOrtholog 1 Ortholog 2

Ortholog 3 Ortholog 4

Fly

A Complex Behavior

Analyze gene expression

Variant A Variant B Variant C Variant D

Gene expression profiles

Ortholog analysis

Functional annotation

PathwaysCorrelated expression

Look for

Study Meta-analyze

Analyze

Generate Hypotheses and design experiments

Co-regulation

Example Insect Behavior

Microarray analysis

Population A Population B Population C

Genes with different expression profiles between populations

Fly and mouse orthologs

Functional annotation

PathwaysCorrelated expression

Look for

Study Meta-analyze

Analyze

Generate Hypotheses and design experiments

Co-regulation

The Fantastic BeeSpace

Type query Entity Identifier

Module Maker

Other entities

Tuned by user feedback

Name and number the modules, describe each

module

Model system Finder

Behavior

Ortholog Search Engine

User approved

User select focal model

system

Experiment Builder

Candidate Gene Bank

Mutant Base

Assay Engineer

Different PnPs

Hypothesis Generator