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The Man, The Myth, The Legend: JGI Gene 2500575882 (Earl) A Novella By: Samantha Simpson

The Man, The Myth, The Legend: JGI Gene 2500575882 (Earl)

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The Man, The Myth, The Legend: JGI Gene 2500575882 (Earl). A Novella By: Samantha Simpson. About Earl. Earl is a 375-bp hypothetical protein that has only been claimed by JGI. Earl decided to do an NCBI blastx search to determine his origins. This only made him more confused. About Earl. - PowerPoint PPT Presentation

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Page 1: The Man, The Myth, The Legend: JGI Gene 2500575882 (Earl)

The Man, The Myth, The Legend: JGI Gene

2500575882 (Earl)

A Novella By:

Samantha Simpson

Page 2: The Man, The Myth, The Legend: JGI Gene 2500575882 (Earl)

About Earl

• Earl is a 375-bp hypothetical protein that has only been claimed by JGI.

• Earl decided to do an NCBI blastx search to determine his origins.

• This only made him more confused.

Page 3: The Man, The Myth, The Legend: JGI Gene 2500575882 (Earl)

About Earl• The top hit for Earl’s left side was FtsZ2,

and the top hit for his right side was CopG.• After performing blastn and refining his

endpoints several times, Earl found out that he shared high similarity with FtsZ2 in bps ranging from 79962..81193 (62% GC Content).

• Earl had high similarity with CopG in bps ranging from 80708..80896 (61% GC Content)

Page 4: The Man, The Myth, The Legend: JGI Gene 2500575882 (Earl)

About Earl

Using FtsZ coordinates in blastx

Using CopG coordinates in blastx v

Page 5: The Man, The Myth, The Legend: JGI Gene 2500575882 (Earl)

About Earl

• Why was FtsZ split when doing a blastx, but it looks fine here?