The Doom and Bloom Making Insulin

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Tuesday, October 18, 2011

The Formula for Making Insulin

Hey Prepper Nation,

One of the questions I am asked most often is how to deal with Type 1 (Insulin-Dependent) diabetics in a

collapse situation. I have been informed that Insulin was prepared in Singapore during WWII, in an effort

to produce medicine for those in the Jewish community that were denied access to Insulin. I have been
http://doctorbonesandamyshow.blogspot.com/http://4.bp.blogspot.com/-IeSmshWG7-8/Tp2iD6CTHnI/AAAAAAAAAU8/NpkuZMzD_H8/s1600/Insulin.jpghttp://www.blogger.com/rearrange?blogID=3819695350795983167&widgetType=Text&widgetId=Text2&action=editWidget&sectionId=crosscol-overflowhttp://4.bp.blogspot.com/-IeSmshWG7-8/Tp2iD6CTHnI/AAAAAAAAAU8/NpkuZMzD_H8/s1600/Insulin.jpghttp://www.blogger.com/rearrange?blogID=3819695350795983167&widgetType=Text&widgetId=Text2&action=editWidget&sectionId=crosscol-overflowhttp://doctorbonesandamyshow.blogspot.com/


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unable to find the specific process used there, but (searching far and wide) I have come upon a post by

SWZ-Ben, moderator at the excellent survivalistboards.com forum. The link to his post is:

http://www.survivalistboards.com/showthread.php?t=33479

In this post, Ben published the original lecture by Frederick Banting who, along with Charles Best,

produced Insulin for the first time in 1922. The lecture is from 1925. Here it is:

"The present method of preparation is as follows. The beef or pork pancreas is finely minced in a larger

grinder and the minced material is then treated with 5 c.c. of concentrated sulphuric acid, appropriately

diluted, per pound of glands. The mixture is stirred for a period of three or four hours and 95% alcohol is

added until the concentration of alcohol is 60% to 70%. Two extractions of the glands are made. The

solid material is then partially removed by centrifuging the mixture and the solution is further clarified

by filtering through paper. The filtrate is practically neutralized with NaOH. The clear filtrate is

concentrated in vacuo to about 1/15 of its original volume.

The concentrate is then heated to 50oC which results in the separation of lipoid and other materials,

which are removed by filtration. Ammonium sulphate (37 grams. per 100 c.c.) is then added to the

concentrate and a protein material containing all the Insulin floats to the top of the liquid. The

precipitate is skimmed off and dissolved in hot acid alcohol. When the precipitate has completely

dissolved, 10 volumes of warm alcohol are added. The solution is then neutralized with NaOH and

cooled to room temperature, and kept in a refrigerator at 5oC for two days. At the end of this time the

dark coloured supernatant alcohol is decanted off. The alcohol contains practically no potency. The

precipitate is dried in vacuo to remove all trace of the alcohol. It is then dissolved in acid water, in which

it is readily soluble. The solution is made alkaline with NaOH to PH 7.3 to 7.5. At this alkalinity a dark

coloured precipitate settles out, and is immediately centrifuged off. This precipitate is washed once or

twice with alkaline water of PH 9.0 and the washings are added to the main liquid.

It is important that this process be carried out fairly quickly as Insulin is destroyed in alkaline solution.

The acidity is adjusted to PH 5.0 and a white precipitate readily settles out. Tricresol is added to a

concentration of 0.3% in order to assist in the isoelectric precipitation and to act as a preservative. After

standing one week in the ice chest the supernatant liquid is decanted off and the resultant liquid is

removed by centrifuging. The precipitate is then dissolved in a small quantity of acid water.

A second isoelectric precipitation is carried out by adjusting the acidity to a PH of approximately 5.0.

After standing over night the resultant precipitate is removed by centrifuging. The precipitate, which

contains the active principle in a comparatively pure form, is dissolved in acid water and the hydrogen

ion concentration adjusted to PH 2.5. The material is carefully tested to determine the potency and is

then diluted to the desired strength of 10, 20, 40 or 80 units per c.c. Tricresol is added to secure a

concentration of 0.1 percent. Sufficient sodium chloride is added to make the solution isotonic. The

Insulin solution is passed through a Mandler filter. After passing through the filter the Insulin is retested
http://www.survivalistboards.com/showthread.php?t=33479http://www.survivalistboards.com/showthread.php?t=33479


3/19

carefully to determine its potency. There is practically no loss in berkefelding. The tested Insulin is

poured into sterile glass vials with aseptic precautions and the sterility of the final product thoroughly

tested by approved methods."

My first impression is that, in a grid-down situation, we are going to be thrown back medically to a time

period before 1922. Banting had access to centrifuges and a number of chemicals that will be

inaccessible to us in a collapse. We might not even be able to produce ice! Searching for ingredients, I

could only find Tricresol (a type of formalin) at dental supply stores; also, what the heck is Berkfelding?

It seems clear that you'll need a lot more than your kid's chemistry set to put this stuff together.

So, what can we do? It seems clear to me that perfect control of our Type 1 diabetics will be highly

unlikely, but it may be possible to prevent life-threatening glucose levels as seen in Diabetic

Ketoacidosis. The strategy I'm discussing is not for use in any circumstance where there are doctors,

hospitals, and modern medical care. It is only my thoughts on what to do in a societal collapse.

Consider stockpiling oral medications such as Metformin in the highest doses available, and place your

diabetics on these when the Insulin runs out. These medicines help counteract Insulin resistance, and

what little Insulin your diabetics produce naturally may have more of an effect to lower blood sugars.

Strict dietary restriction barely enough to maintain weight will also be necessary, in the form of

frequent, very small meals. Maintain a reasonable level of activity, and hope for society to restabilize.

Use whatever natural supplements might have an effect, such as Chromium, Magnesium and ionic

Vanadium. Herbs that purport to have an effect in lowering sugar levels include Cinnamon, Fenugreek,

Bitter Melon, and American Ginseng. I can't promise you glucose control, however, as I have no

experience with them as a diabetic treatment.

With regards to major medical issues such as Diabetes, we will have to improvise in an effort to keep our

people free of life-threatening Ketoacidosis. In a collapse, we will have to realize that, sometimes,

something is better than nothing.

Dr. Bones

Posted by Dr. Bones and Nurse Amy at 12:07 PM

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Labels: alternative medicine, collapse medicine, DIABETES, Dr Bones and Nurse Amy's Doom and BloomShow, Insulin, Insulin formula, preparedness, survival

Reactions:

2 comments:
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millenniumfly said...

Trying to follow that formula sounds like a recipe for disaster! I would suspect the layman (like me)

would easily get something wrong and do more harm than good. Thanks for sharing anyway.

October 19, 2011 9:36 AM

Dr. Bones and Nurse Amy said...

Hi Millenium,

It just goes to show how complex the pharmaceutical manufacturing process is, and all the chemicals

that are involved....

Thanks for the comment!

Dr. Bones

October 19, 2011 4:47 PM

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7/19

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8/19

SUTURE KITS AND COLLAPSE MEDICINE(tm) DVD NOW AVAILABLE!

Dr. Bones and Nurse Amy are offering a suture kit package, which includes suturing instruments, nitrile

gloves, practice sutures and instruction cards. Follow along with "How To Suture with Dr. Bones" on

YouTube. A DVD of our Collapse Medicine(tm) presentation is also available. Each product is $20 plus

shipping. Email [email protected] to order!

LISTEN TO OUR PODCAST !! CLICK HERE:

Prepperpodcast on Blogtalk Radio

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Making Penicillin at Home
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9/19

Hey Prepper Nation, Accumulating medications may be simple when it comes to finding aspirin and

other non-prescription drugs but pres...

The Formula for Making Insulin

Hey Prepper Nation, One of the questions I am asked most often is how to deal with Type 1 (Insulin-

Dependent) diabetics in a collapse sit...

Bug Out Bag Medical Kit with Natural Remedies

Bug Out Bag Medical Supplies (in larger size containers) A bug out bag can be a life-saving preparation

for emergencies, including ...

Thyroid Disease in a Collapse

Hey Prepper Nation, The thyroid gland is positioned just in front of the Adams Apple and produces

hormones that help regulate your met...

The Truth About Expiration Dates

Hey Preppers, I get a lot of questions about expiration dates on medications and whether medicationsshould be thrown out once they hit t...
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10/19

The 7 Deadly Sins

Hey Prepper Nation, It's time to talk about the 7 deadly sins, sins that will lead to damnation for those

foolish enough to commit them i...

Cellulitis: An Epidemic in a Collapse

Hey Prepper Nation, In a collapse situation, we will be required to perform many outdoors activities we

are not used to. We'll be choppin...

FISH ANTIBIOTICS IN A COLLAPSE

Hey Preppers, Accumulating medications for a possible collapse may be simple when it comes to getting

Ibuprofen and other non-prescriptio...

Natural First Aid Kit Part 1: Essential Oils

Essential Oil facility Some of Nurse Amy's essential oils If you really believe a collapse is going to happen

then preparing for ...

Natural First Aid Kit Part 2: Essential Oils continued

A few of Nurse Amy's essential oils Nature may, one day, be our pharmacy. Not too long ago our healing

medicines came from the land aro...

Our youtube channel
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11/19

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12/19

Listen to internet radio with Prepper Podcast on Blog Talk Radio

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Stop bleeding with Quikclot and the Israeli Trauma Dressing 10/19/11

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TEOTWAWKI Blog

Zombie Contest Entry #14

57 minutes ago

Something Wicked Comes

Filling Your Plate: Part IV - Crock Pot Recipes

2 hours ago
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13/19

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Tips for saving seeds & a giveaway!

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The Doom and Bloom Making Insulin