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The Cell Cycle
Key Concepts
• Most division results in genetically identical cells
• Cell cycle consists of alternating periods of mitosis and interphase
• Eukaryotic cell cycle is highly regulated
Purpose
• Ultimate purpose of cell cycle is to propagate genetic information
• Also important for– Tissue repair – Growth – Reproduction
Figure 12.5-1
ChromosomesChromosomal
DNA molecules
Centromere
Chromosomearm
1
Figure 12.5-2
ChromosomesChromosomal
DNA molecules
Centromere
Chromosomearm
Chromosome duplication(including DNA replication)and condensation
Sisterchromatids
1
2
Figure 12.5-3
ChromosomesChromosomal
DNA molecules
Centromere
Chromosomearm
Chromosome duplication(including DNA replication)and condensation
Sisterchromatids
Separation of sisterchromatids intotwo chromosomes
1
2
3
Phases of cell cycle
• Interphase– G1– G2– S
• Mitosis– Prophase– Metaphase– Anaphase– Telophase
Figure 12.6
INTERPHASE
G1
G2
S(DNA synthesis)
MITOTIC(M) PHASE
CytokinesisM
itosis
Figure 12.7
G2 of Interphase Prophase Prometaphase
Centrosomes(with centriole pairs)
Chromatin(duplicated)
Nucleolus Nuclearenvelope
Plasmamembrane
Early mitoticspindle
AsterCentromere
Chromosome, consistingof two sister chromatids
Fragments of nuclearenvelope
Nonkinetochoremicrotubules
Kinetochore Kinetochoremicrotubule
Metaphase
Metaphase plate
Anaphase Telophase and Cytokinesis
Spindle Centrosome atone spindle pole
Daughterchromosomes
Cleavagefurrow
Nucleolusforming
Nuclearenvelopeforming
10
m
Figure 12.7a
G2 of Interphase Prophase Prometaphase
Centrosomes(with centriole pairs)
Chromatin(duplicated)
NucleolusNuclearenvelope
Plasmamembrane
Early mitoticspindle
AsterCentromere
Chromosome, consistingof two sister chromatids
Fragments of nuclearenvelope
Nonkinetochoremicrotubules
Kinetochore Kinetochoremicrotubule
Figure 12.7b
Metaphase
Metaphase plate
Anaphase Telophase and Cytokinesis
Spindle Centrosome atone spindle pole
Daughterchromosomes
Cleavagefurrow
Nucleolusforming
Nuclearenvelopeforming
Mitotic Spindle
Sisterchromatids
AsterCentrosome
Metaphaseplate(imaginary)
Kineto-chores
Overlappingnonkinetochoremicrotubules Kinetochore
microtubules
Microtubules
Chromosomes
Centrosome
0.5 m
1 m
Figure 12.9a
Kinetochore
Mark
Spindlepole
EXPERIMENT
RESULTS
Figure 12.9b
Chromosomemovement
Microtubule
Motor protein
Chromosome
Kinetochore
Tubulinsubunits
CONCLUSION
Cytokenisis
Figure 12.10a(a) Cleavage of an animal cell (SEM)
Cleavage furrow
Contractile ring ofmicrofilaments
Daughter cells
100 m
Figure 12.10b (b) Cell plate formation in a plant cell (TEM)
Vesiclesformingcell plate
Wall of parent cell
Cell plate New cell wall
Daughter cells
1 m
Binary Fission
• Prokaryotes replicate via binary fission• Genome replication starts at location called
origin of replication• Cell membrane pinches around midline of cell
Figure 12.12-1
1
Origin ofreplication
E. coli cell
Two copies of origin
Cell wallPlasma membrane
Bacterial chromosomeChromosomereplicationbegins.
1
Origin ofreplication
E. coli cell
Two copies of origin
Cell wallPlasma membrane
Bacterial chromosome
Origin Origin
Chromosomereplicationbegins.
Replicationcontinues.
2
Figure 12.12-2
1
Origin ofreplication
E. coli cell
Two copies of origin
Cell wallPlasma membrane
Bacterial chromosome
Origin Origin
Chromosomereplicationbegins.
Replicationcontinues.
Replicationfinishes.
2
3
Figure 12.12-3
1
Origin ofreplication
E. coli cell
Two copies of origin
Cell wallPlasma membrane
Bacterial chromosome
Origin Origin
Chromosomereplicationbegins.
Replicationcontinues.
Replicationfinishes.
Two daughtercells result.
2
3
4
Figure 12.12-4
Evolution of mitosis
• Eukaryotes most likely evolved from prokaryotic organisms
• Some organisms display intermediate “levels” of mitotic behavior.
Cell Cycle RegulationG1 checkpoint
G1
G2
G2 checkpoint
M checkpoint
M
SControlsystem
Figure 12.15
Cyclins and CDKs
• Two types of regulatory proteins in cell cycle control: cyclins and cyclin-dependent kinases (Cdks)
• Cdks activity fluctuates during cell cycle because it is controled by cyclins, so named because their concentrations vary with the cell cycle
• MPF (maturation-promoting factor) is a cyclin-Cdk complex that triggers a cell’s passage past the G2 checkpoint into the M phase
Figure 12.17a
(a) Fluctuation of MPF activity and cyclin concentration during the cell cycle
MPF activity
Cyclinconcentration
Time
M M MS SG1 G2 G1 G2 G1
(b) Molecular mechanisms that help regulate the cell cycle
Cdk
Degradedcyclin
Cyclin isdegraded
MPF
G2checkpoint
Cdk
Cyclin
M
S
G 1
G 2
Figure 12.17b
Stop and Go Signals
• An example of an internal signal is that kinetochores not attached to spindle microtubules send a molecular signal that delays anaphase
• Some external signals are growth factors, proteins released by certain cells that stimulate other cells to divide
• For example, platelet-derived growth factor (PDGF) stimulates the division of human fibroblast cells in culture
Figure 12.18
A sample of humanconnective tissue iscut up into smallpieces.
Enzymes digestthe extracellularmatrix, resulting ina suspension offree fibroblasts.
Cells are transferred toculture vessels.
Scalpels
Petridish
PDGF is addedto half thevessels.
Without PDGF With PDGF
10 m
1
2
34
External Signals
• A clear example of external signals is density-dependent inhibition, in which crowded cells stop dividing
• Most animal cells also exhibit anchorage dependence, in which they must be attached to a substratum in order to divide
• Cancer cells exhibit neither density-dependent inhibition nor anchorage dependence
Cancer
• Normal cells undergo a transformation • Typically result of genetic mutation• Loss of ability to govern cell cycle
– Too much growth – Not enough death
• Cancers typically pick up more and more mutations as they progress.