The Cell

Observed by Light and Electron Microscopy

Overview of the Cellular Organelles

Light Microscopy – Hematoxylin & Eosin (H&E) Stain

Basophilic (blue) Nuclei

Eosinophilic (red)

cytoplasm

Colorized low mag TEM of a cell

Red = Mitos

Blue = Ribos

Green = RER

Colorized TEM of a cell nucleus

Arrow = Nucleolus

Red = Mitos

Green = RER

Nuclear Structures

Low mag TEM of a nuclei

Nuclear pores (arrows) Nuclear pores (en face)

Colorized TEM of nuclear pores (blue) en face

High mag TEM of a nucleolus

Pars fibrosa/granulosa (dense black) and

fibrillar center (arrow)

TEM of interphase nuclei and a mitotic cell with condensed chromosomes (arrow)

Cytoplasmic Structures

Ribosomes, RER and Glogi

Ribosomes by light microscopy = basophilic cytoplasm

TEMs of free ribosomes – 25 nm granules – in clusters called polysomes

Colorized TEM of polysomes (yellow highlighted)

Low mag TEM of rough endoplasmic reticulum (RER)

Colorized higher mag TEM of RER (green)

TEM of RER – arrows indicate cisternae

Low mag TEM of RER and Golgi (Go)

Colorized TEM of a Golgi system

TEM of a Golgi system

TEM of lysosomes (arrows) & a residual body

Residual Body

Cytoplasmic inclusions by Light Microscopy

Basophilic granules (top picture)

Eosinophilic granules (bottom picture)

Mitochondria

Colorized TEM of Mitochrondria (red)

TEMs of Mitochrondrial shapes

TEM of a Mitochrondrion

Arrows = cristae

The Cytoskeleton

Light microscopy of mitotic spindles = Microtubules

Colorized TEM of Cilia in cross section showing microtubules

Immunofluorescence of Microtubules in cultured cells (green)

TEM of Microfilaments (arrows)

Immunofluorescence of microfilament stress fibers in cultured cells

Cell Membrane

High mag TEM of two cell membranes (unit membrane)

Colorized TEM of two cell membranes (unit membrane)

TEMs of membrane specializations

Top 4 = microvilli

Middle = Stereo cilia

Bottom = Cilia

TEM of Cilia (C) with microtubular cores forming from basal

bodies (BB)

High mag TEM of Cilia with

microtubular cores (Fil) with the basal body (FiC) at top of

image