THE

BIOCHEMICAL

JOURNAL

1 September 1980 Volume 189, No. 3

EDITORIAL BOARD

ChairmanJ. T. Dingle

Deputy ChairmenC. 1. PogsonA. J. KennyJ. B. ClarkA. J. Comish-BowdenJ. A. Lucy

Editorial ManagerA. G. J. Evans

Editorial SecretaryA. S. Beedle

P. M. BayleyW. J. BrammarR. C. BrayJ. W. BridgesD. N. BrindleyH. G. BrittonK. BrocklehurstM. J. ClemensB. A. CookeD. D. DaviesR. T. DeanR. M. DentonF. M. DickinsonG. J. DuttonP. C. EngelJ. E. FothergillP. J. GarlickJ. L. GordonD. E. Griffiths

L. A. GrivellB. A. HaddockL. Hue0. T. G. JonesP. LundR. M. MarchbanksG. MurphyN. B. MyantK. B. M. ReidD. RobinsonD. SchulsterJ. E. ScottS. P. Spragg*D. R. StanworthM. J. A. TannerP. TulkensR. T. Walker

*Nominated by the BritishBiophysical Society

OverseasAdvisory PanelH. Beinert (U.S.A.), H. F. DeLuca (U.S.A.), W. Fiers (Belgium), R. W. Hanson (U.S.A.),O. Hayaishi (Japan), B. Hess (Germany), D. B. Keech (Australia), T. C. Laurent (Sweden),L. F. Leloir (Argentina), R. Ross (U.S.A.), M. Schramm (Israel), A. Tissi6res (Switzerland),D. Trentham (U.S.A.), A. D. Vinogradov (U.S.S.R.), 0. Wieland (Germany)

34 London: The Biochemical Society @1 980i ,

THE BIOCHEMICAL SOCIETY

OFFICERS AND COMMITTEE, 1980-81Chairman of the CommiltteeS. V. Perry, F.R.S.TreasurerD. F. ElliottAssistant TreasurerB. SpencerGeneral SecretaryD. RobinsonPublications SecretaryG. B. AnsellMeetings SecretaryH. F. Bradford

CommitteeJ. R. BronkE. G. BrownP. H. Clarke, F.R.S.J. T. Dingle*tP. F. FottrellM. I. GurrJ. L. HarwoodF. W. HemmingJ. A. LucyR. D. MarshallJ. Mowbray*tI. H. M. Muir, F.R.S.

J. H. OttawayR. N. PerhamC. F. Phelps

*Ex officio Member of CommitteetRepresentative of Editorial Boardof the Biochemical Journal.

tRepresentative of Group Secre-taries.

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(ii)

CONTENTS

INDEX OF AUTHORS

PAGE

441553653

625491655467421475615467641641

435635467461655435

605591501645649501

Adair, W. L., Jr.Adiga, P. R.Andersen, B.

Bengtsson, G.Berg, R. A.Biosca, J. A.Blakemore, W. F.Boiteux, A.Bollen, I. C.Bray, R. C.Burditt, L. J.Butler, J.Buxton, G. V.

Caputto, R.Chevaillier, P.Chotai, K.Cook, J. S.Cuchillo, C. M.Cumar, F. A.

Daniels, M.Danielsen, E. M.Deakin, J. F. W.De Matteis, F.Dopheide, T. A.Dostr6vsky, J. 0

Edgerton, M. E.Ellis, S. A.Evans, R. W.

Finlay, T. H.Florence, T. M.Fujiwara, M.

Gibbs, A. H.Gleeson, P. A.Goodall, A. A.Greenwood, C.Gutteridge, S.

Harrington, P. C.Hayaishi, 0.

Hess, B.Higgins, J. A.Hirani, S.H66k, M.

Ingram, J.Itano, R.Itano, T.

Jain, K. M.

Kamoda, N.Kao, W. W.-YKedersha, N. L.Kellett, G. L.

Kenny, A. J.Kido, R.Knight, C. G.

Lindahl, U.Longas, M. 0.

Maggio, B.Makino, M.

413 Malcovati, M.

605 Mallik, B.

541 Markus, M.

Marshall, R. D.481 Minatogawa, Y.507 Misra, T. N.393 Moore, T. A.

PAGE

645649533413615

641393421475467625

591455455

547

581491491

561, 569591581447

625481

435581421547421533581547413

Nakamura, M.Nakanishi, J.Noren, 0.

Nugent, P. G.

Okuno, E.Olivecrona, T.

Penniston, J. T.Philippe, M.Plesser, T.Pogson, C. I.

Riesenfeld, J.Roberts, D.Robertson, S.

Schwarting, G. A.Sellers, A.Sj6str6m, H.Smith, A. G.Smith, S. A.Symth, D. G.Srivenugopal, K. S.Sykes, A. G.

Wallis, M.Ward, C. W.Watanabe, Y.Wilkins, R. G.Williams, J.Winchester, B. G.Woessner, J. F., Jr.Wood, P. M.

Yoshida, R.

PAGE

581581591467

581625

455635421461

625561, 569

441

407521591645464501553641

605649393641541467521385

393

PHOTOCOPYING

The appearance of the code at the bottom of the Box 765, Schenectady, New York 12301, U.S.A.)first page of an article in this journal indicates the for copying beyond that permitted by Sections 107copyright owner's consent that copies of the article or 108 of the U.S. Copyright Law. This consentmay be made in the U.S.A. for personal or internal d nuse, or for the personal or internal use of specific coes for gen er kmds for sing orclients. This consent is given on the condition, copying for general distribution, for advertising orhowever, that the copier pay the stated per-copy fee promotional purposes, for creating new collectivethrough the Copyright Clearance Center Inc. (P.O. works, or for resale.

v

NOTICE FOR CONTRIBUTORSThe Biochemical Journal places emphasis on the prompt publication of both full-length papers (on average about 6 months

after receipt) and rapid papers (on average 10-12 weeks after receipt).For detailed instructions on the preparation of papers contributors (who need not be members of the Biochemical Society)

should refer to Policy ofthe Journal and Instructions to Authors [Biochem. J. (1978) 169, 1-27], and the notice below.Papers submitted for publication should be addressed to the Editorial Manager, Biochemical Journal, 7 Warwick

Court, London WC1R 5DP.Contributors should note that the Biochemical Journal makes no manuscript handling charges, no page charges and

no charges for plates. Reprints are available at modest cost at about the same time as publication, and, if an author is amember of the Biochemical Society, 25 reprints are provided free of charge.

The Biochemical Journal accepts papers on all aspects of Biochemistry. Alternate issues of the journal are devoted toMolecular Aspects and Cellular Aspects. For the convenience of readers, papers within each issue are assigned to sectionson the Contents pages. Authors should indicate whether their papers are Molecular or Cellular and designate the sectionin Contents in which they would like their paper to appear. Section headings, for both Molecular Aspects and Cellular Aspects,are reviewed from time to time; the present headings are as follows:

Molecular Aspects (Physical, Structural and ChemicalProperties of Biochemical Systems, including SequencingInformation)Peptide and Protein StructureEnzymes and Enzyme KineticsMetalloproteinsNucleotides, Nucleic Acids and NucleoproteinsLipidsMembranesCarbohydrates and Complex CarbohydratesPhysical Biochemistry

Cellular Aspects (Biochemical Properties of Metabolic,Subcellular and Cellular Systems)Protein Biosynthesis/Molecular GeneticsProtein TurnoverMetabolism, Regulation and Control ProcessesCell Surfaces and ReceptorsDevelopmental BiochemistryMembranes, Transport, Bioenergetics and Photosynthesis

(vi)

IMPORTANT NOTICE FOR AUTHORSLimitation ofpaper lengths. As a measure to meet the effects of continuously rising production costs ofthe Journal the Editorial Board has decided to introduce a policy of limitation of length of publishedpapers. In future papers should not normally exceed eight printed pages in the Journal, includingFigures and Tables. (Eight pages represent approximately 5000 words without Figures or Tables; as aguide, the number of double-spaced A4 typescript sheets for text and references, with the addition ofone sheet for each Figure, Table or Scheme with its legend, should not exceed 24 if the eight printedpages maximum is to be met.) Cooperation of authors in this will be expected, and there is anadvantage in that shorter papers are usually published with the least delay. Authors should also notethat a single paper consisting justifiably of ten printed pages, for example, will normally be consideredmore favourably than two papers each of six pages dealing with the same material.Copies of submitted papers. To expedite handling and refereeing procedures in a new system to beoperated by the Journal authors are now requested to provide three copies of papers at submission.New features. In 1981 the inclusion of Reviews in the Journal will commence; these will usually beinvited, although prospective authors of Reviews are welcome to contact the Deputy Chairmanconcerned with Reviews (Professor J. A. Lucy) at the Editorial Office.'Biochemical Journal Letters' is an additional feature now being implemented for the Journal. 'BJLetters' are intended to provide an opportunity to discuss, criticize or expand particular points made inpublished work, or to present a hypothesis. They must be concisely written and each will normallyoccupy less than one printed page of the Journal. Letters will be treated as items for rapid publication.They will be accepted only if they are thought to represent soundly reasoned opinions. Letters are notintended to provide a vehicle for discussion of more general matters which might more appropriately besent to the Biochemical Society Bulletin. If a letter is polemical in nature, a reply may be solicited fromother interested parties before its publication. Such replies will be assessed for publication with theoriginal letter if this is appropriate. Letters should be sent to the Editorial Office.

INDEX OF AUTHORS

Index of Authors

Aasted, B.Adair, W. L., Jr.Adiga, P. R.Andersen, B.Aplin, J. D.

Bailey, A. J.Baines, B. S.Bandyopadhyay, A. K.Barber, D.Barrett, A. J. 17-Bengtsson, G.Ben-Yoseph, Y.Berg, R. A.Biosca, J. A.Blakemore, W. F.Boiteux, A.Bollen, I. C.Bond, J. S.Bourrillon, R.Bray, R. C.Brocklehurst, K.Burchell, B.Burditt, L. J.Butler, J.Buxton, G. V.

Cammarano, P.Campbell, R. D.Caputto, R.Cawston, T. E.Charles, S. A.Chatterjee, N. K.Chevaillier, P.Chotai, K.Cook, J. S.Cuchillo, C. M.Cumar, F. A.

Daniels, M.Danielsen, E. M.Davies, G. E. 263-,Deakin, J. F. W.Dean, P. D. G.De Matteis, F.Dobrovolskii, A. B.Dodds, A. W.Dopheide, T. A.Dostr6vsky, J. 0.

Edgerton, M. E.Ellis, S. A.Evans, R. W.

Felsani, A.Finlay, T. H.Florence, T. M.Fluharty, A. L.Fujiwara, M.

Vol. 189

PAGES183-184441-445553-560653-654337-347

111-124189-19289-93

285-294-25, 255-261

625-6339-15

491-499655-657467-473421-433475-480

17-25185-188615-623189-192377-380467-473641-644641-644

313-33567-80

435-440349-357373-376143-152635-639467-473461-466655-657435-440

605-614591-603

275, 277-284501-506

27-34645-648219-226

67-80649-652501-506

413-420605-614541-546

313-335481-489507-52045-49

393-405

PAGESGautvik, K. M. 153-159Gibbs, A. H. 645-648Gietzen, K. 81-88Gleeson, P. A. 649-652Gonzalez-Duarte, R. 105-110Goodall, A. A. 533-539Grand, R. J. A. 227-240Greenwood, C. 285-294,413-420Griffiths, W. T. 125-133Grinich, N. P. 1-8Gull, K. 305-312Gusev, N. B. 219-226Gutteridge, S. 615-623

Hachey, C. 135-142Hales, B. F. 135-142Halliwell, B. 373-376Harding, S. E. 359-361Harrington, P. C. 641-644Hayaishi, 0. 393-405Henderson, C. E. 161-172Hess, B. 421-433Higgins, J. A. 475-480Hirani, S. 467-473H66k, M. 625-633Howell, R. M. 209-218Hughes, R. C. 337-347Hungerford, M. 9-15

Ingram, J. 591-603Itano, R. 455-459Itano, T. 455-459

Jain, K. M. 547-552Johansen, L. 153-159Johnson, M. K. 285-294Juan,E. 105-110

Kainuma, K. 381-383Kamoda, N. 581-590Kao, W. W.-Y. 491-499Karpetsky, T. P. 263-275, 277-284Kedersha, N. L. 491-499Kellett, G. L. 561-567,569-579Kenny, A. J. 591-603Kerr, M. A. 173-181Kido, R. 581-590Kihara, H. 45-49Kinsella, J. E. 363-366Kivirikko, K. I. 247-253Knight, C. G. 447-453Koch, J. 95-104

Leader, D. P. 241-245Leatherbarrow, R. J. 27-34Levy, C. C. 89-93, 263-275,

277-284Light, N. D. 111-124Lindhahl, U. 625-633

Londei, P.Longas, M. 0.

Macfarlane, C. C.Maggio, B.Makino, M.Malcovati, M.Mallik, B.Mapleston, R. E.Markus, M.Marshall, R. D.Mazzei, F.McCrae, S. I.McDonagh, A. F.Miller, R. T.Mills, G.Minatogawa, Y.Misra, T. N.Moore, T. A.Mushiri, M. S.

Nadler, H. L.Nakamura, M.Nakanishi, J.Noren, 0.Nugent, P. G.Nustad, K.

Okuno, E.Olivecrona, T.0rstavik, T. B.

Palma, L. A.Pena, S. D. J.Penniston, J. T.Perham, R. N.Perry, S. V.Petering, D. H.Phillipe, M.Plesser, T.Pogson, C. I. 3Porter, R. R.Prigent, M.-J.Puistola, U.

Raggi, A.Ranieri-Raggi, M.Rezvan, H.Riding, G. A.Riesenfeld, J.Robaire, B.Roberts, D. 5Robertson, S.Roobol, A.

Sarma, M. H.Saryan, L. A.Sasaki, T.Sayers, C. A.

PAGES313-335481-489

51-65435-440581-590421-433547-552125-133421-433533-539313-335

51-65193-20845-49

337-347581-590547-552413-420189-192

9-15581-590581-590591-603467-473153-159

581-590625-633153-159

193-208337-347455-459161-172227-24095-104

635-639421-433

05-312,461-46667-80

185-188247-253

367-368367-368209-218295-303625-633135-142

561-567,569-579441-445305-312

143-15295-104

381-383255-261

659

INDEX OF AUTHORS

Schwarting, G. A.Schwarz, L. R.Sellers, A.Severin, S. E.Shankara, B.Shaw, C. F.Shetty, J. K.Shriver, K. K.Singh, J.Sj6str6m, H.Smith, A. G.Smith, S. A.Smyth, D. G.Srivenugopal, K. S.Stevens, R. L.

PAGES

407-412369-372521-531219-22695-10495-104363-366277-284369-372591-603645-648461-466501-506553-56045-49

Svindahl, K.Sykes, A. G.

PAGES

153-159641-644

Tejcka, M. 81-88Terwilliger, R. C. 1-8Thomson, A. J. 285-294Turpeenniemi-Hujanen, T. M.

247-253Tyler, J. A. 349-357

Wallis, M.Walsh, T. A.Wang, D.Ward, C. W.Watanabe, Y.

605-614285-294

89-93649-652393-405

Weatherill, P. J.Weaver, R. C.Wiebel, F. J.Wielgus, S.Wilkins, R. G.Willadsen, P.Williams, J.Winchester, B. G.Woessner, J. F., Jr.Wolf, H. U.Wolosin, J. M.Wood, P. M.Wood, T. M.

Yoshida, R.

PAGES

377-380363-366369-37295-104

641-644295-303541-546467-473521-531

81-8835-44

385-39151-65

393-405

1980

660

INDEX OF SUBJECTS

Index of SubjectsAdenosine triphosphatase

Ca2+-plus-Mg2+-dependent 81-88erythrocyte-membrane 81-88

Adenosine triphosphateJ,N6-etheno-, see 1,N6-Ethenoadenosine triphosphate

Adenylate deaminaseskeletal-muscle 367-368

Agarosesodium dodecyl sulphate/polyacrylamide-gel electro-

phoresis and 183-184Alanine-glyoxylate aminotransferase

liver 581-590Albumin, serum, see Serum albuminAlcohol dehydrogenase

fruitfly 105-1 10Aldolase, see Fructose bisphosphate aldolaseAmidinotransferase

pulse-seedling 553-560Aminopeptidase A, see Aspartate aminopeptidaseAnhydrotrypsin

cs-macroglobulin and 255-261Anion-transport system

erythrocyte-membrane 35-44Antibody-antigen aggregates

complement component C4 and 67-80Antigen-antibody aggregates

complement component C4 and 67-80Antithrombin III

thrombin and 481-489fi-Apo-8'-carotenal

charge-transfer complexes of 547-552Arylsulphatase A

urinary 45-49Ascites-tumour cells

Ehrlich, see Ehrlich ascites-tumour cellsAspartate aminopeptidase

brush-border-membrane 591-603kidney-cortex 591-603

Astacenecharge-transfer complexes of 547-552

Bacillus stearothermophiluspyruvate dehydrogenase complex of 161-172

Barbiata reeveana, see ClamBarley (Hordeum vulgare) seedlings

etioplasts of 125-133protochlorophyllide reductase of 125-133

BHK cellsribosomal protein S3 of 241-245

Bile saltscerebroside sulphatase and 45-49

Bilirubinbiliverdin IXa synthesis from 193-208

Biliverdin IXasynthesis of 193-208

Blood plasmafibronectin of 337-347

Blood serumtransferrin of 541-546

Boophilus microplus, see Tick

Braincalmodulin of 227-240galactocerebrosidase of 9-15thromboplastin of 209-218

Brush-border membraneaspartate aminopeptidase of 591-603kidney-cortex 591-603

Cadmiummetallothionein binding of 95-104

Calcium ionsgangliosides and 435-440

Calmodulinbrain 227-240Ca2+-plus-Mg2+-dependent adenosine triphosphatase

and 81-88histone H2B and 227-240histones and 455-459myelin basic protein and 227-240,455-459polyarginine and 455-459polylysine and 455-459protamine and 455-459

Carica papaya, see Papayaall-trans-,B-Carotene

charge-transfer complexes of 547-552Cartilage

proteoglycan of 521-531Cathepsin B

leupeptin and 447-453liver 17-25, 447-453lysosomal 17-25

CellobiohydrolasePenicilliumfuniculosum 51-65

CellulasePenicilliumfuniculosum 51-65

Cellsascites-tumour, see Ascites-tumour cellsbaby-hamster kidney, see BHK cellsHeLa, see HeLa cells

Cerebroside sulphatasebile salts and 45-49urinary 45-49

Chick embryolysyl hydroxylase of 247-253

Chick-embryo tendon fibroblastsprolyl hydroxylase of 491-499

Chickenoviduct dolichol of 441-445

Chicken eggovotransferrin of 541-546

Chloroplastsfructose bisphosphatase of 373-376spinach-leaf 373-376

Chromatium vinosumhigh-potential iron-sulphur protein of 641-644

Chymopapain A2,2'-dipyridyl disulphide and 189-192papaya 189-192

Chymotrypsindisulphide bonds of 507-520

Vol. 189

661

INDEX OF SUBJECTS

Chymotrypsinogendisulphide bonds of 507-520

Cibacron Blue F3G Aserum albumin and 27-34

Clam (Barbiata reeveana)haemoglobin of 1-8

Clam (Macrocallista chione)ribosomal ribonucleic acids of 313-335

Clearing-factor lipase, see Lipoprotein lipaseCollagen

polymeric C-terminal cross-linked peptide from 111-124

tendon 111-124type-I 111-124

Collagenasesynovial 349-357

Complement component C3 convertaseassembly of 173-181

Complement component C4antibody-antigen aggregates and 67-80

Coppermetallothionein binding of 95-104

Cyclic cytidine 2': 3'-monophosphate, see Cytidine 2': 3'-cyclic monophosphate

Cytidine 2': 3'-cyclic monophosphateribonuclease A and 655-657

Cytochrome clRhodopseudomonas sphaeroides 385-391

Deoxyribonucleic acid polymerasepolyspermine-ribonuclease and 89-93Rauscher-leukaemia-virus 89-93RNA-dependent 89-93

Deoxyribonucleic acid polymerasesdogfish 635-639testis 635-639

Dermatan sulphatelipoprotein lipase and 625-633

2,2'-Dipyridyl disulphidechymopapain A and 189-192

Disulphide bondsprotein 507-520

Dogfish (Scylliorhinus caniculus)testicular deoxyribonucleic acid polymerases of 635-

639Dolichol

liver 441-445oviduct 441-445

Drosophila melanogaster, see FruitflyDrosophila simulans, see FruitflyDrosophila virilis, see Fruitfly

Ehrlich ascites-tumour cellsmetallothioneins of 95-104

Electrophoresissodium dodecyl sulphate/polyacrylamide-gel 183-

184Endoplasmic-reticulum membranes

liver 475-480phenobarbital administration and 475-480phospholipids of 475-480

f-Endorphinpituitary-gland 501-506

Epididymisglutathione S-transferases of 135-142

Erythrocyte membraneanion-transport system of 35-44Ca2+_plus_Mg2+-dependent adenosine triphosphatase

of 81-88Escherichia coli

pyruvate kinase of 421-4331,N6-Ethenoadenosine triphosphate

phosphofructokinase and 561-567, 569-579Etioplasts

barley-seedling 125-133protochlorophyllide reductase of 125-133wheat-seedling 125-133

Fibrinogensubfragment of 359-361

Fibronectinplasma 337-347

Fructose bisphosphatasechloroplast 373-376hydrogen peroxide and 373-376spinach-leaf 373-376

Fructose bisphosphate aldolasedegradation of 17-25skeletal-muscle 17-25

Fruitfly (Drosophila melanogaster)alcohol dehydrogenase of 105-110

Fruitfly (Drosophila simulans)alcohol dehydrogenase of 105-110

Fruitfly (Drosophila virilis)alcohol dehydrogenase of 105-110

Fumarate hydrataseheart 653-654

Galactocerebrosidasebrain 9-15Krabbe-disease 9-15liver 9-15

Gangliosidescalcium ions and 435-440

Globoid-cell leucodystrophy, see Krabbe diseaseGlutathione S-transferases

epididymal 135-142vas deferens 135-142

GlycoproteinTamm-Horsfall, see Tamm-Horsfall glycoprotein

Glycosphingolipidslymphocyte 407-412phospholipids and 435-440

Haemagglutinininfluenza-virus 649-652oligosaccharide units of 649-652

Haemoglobinclam (Barbatia reeveana) 1-8

Haemoproteinsnitrosyl- 285-294

Halobacterium halobiumpurple membrane of 413-420

Heartfumarate hydratase of 653-654

1980

662

INDEX OF SUBJECTS

HeLa cellscyclic nucleotide-independent protein kinases of 143-

152polyribosomes of 143-152

Hen, see ChickenHeparan sulphate

lipoprotein lipase and 625-633Heparin

lipoprotein lipase and 625-633Histone H2B

calmodulin and 227-240Histones

calmodulin and 455-459Homoarginine

metabolism of 553-560pulse-seedling 553-560

Hordeum vulgare, see BarleyHydrogen peroxide

fructose bisphosphatase and 373-376

Influenza virushaemagglutinin of 649-652

Indole 2,3-dioxygenasekidney 461-466small-intestinal 461-466spleen 461-466stomach 461-466

Intestine, small, see Small intestineIpomoea batatas, see Sweet potatoIron-sulphur protein

Chromatium vinosum 641-644high-potential 641-644

Kallikreinssubmandibular-gland 153-159

Kidneyindole 2,3-dioxygenase of 461-466

Kidney cells, baby-hamster, see BHK cellsKidney cortex

aspartate aminopeptidase of 591-603brush-border membrane of 591-603

Krabbe diseasegalactocerebrosidase of 9-15

Kynurenine-glyoxylate aminotransferaseliver 581-590

Lathyrus sativus, see PulseLeaves

spinach 373-376Lectin

anti-(blood-group N) 185-188Vicia graminea 185-188

Leukaemia virusRauscher, see Rauscher leukaemia virus

Leupeptincathepsin B and 447-453

Lipaseheparin-like polysaccharides and 625-633liver 625-633

Lipoprotein lipaseheparin-like polysaccharides and 625-633milk 625-633

Livercathepsin B of 17-25, 447-453dolichol of 441-445endoplasmic-reticulum membranes of 475-480galactocerebrosidase of 9-15kynurenine-glyoxylate aminotransferase of 581-590lipase of 625-633microsomal membranes of 475-480protohaem ferro-lyase of 645-648tryptophan 2,3-dioxygenase of 393-405uridine diphosphate glucuronate-oestrone

glucuronosyltransferase of 377-380uridine diphosphate glucuronate-testosterone

glucuronosyltransferase of 377-380Loligo vulgaris, see SquidLymphocytes

glycosphingolipids of 407-412Lysosomes

cathepsin B of 17-25liver 17-25

Lysyl hydroxylasechick-embryo 247-253

Macrocallista chione, see Clama2-Macroglobulin

anhydrotrypsin and 255-261Macromolecules

conformation of 359-361a-D-Mannosidase

mannosidosis and deficiency of 467-473Mannosidosis

a-D-mannosidase deficiency in 467-473Membrane

brush-border, see Brush-borderendoplasmic-reticulum, see Endoplasmic-reticulum

membraneerythrocyte, see Erythrocyte membranemicrosomal, see Microsomal membranepurple, see Purple membrane

Metallothioneincadmium-binding 95-104copper-binding 95-104Ehrlich-ascites-tumour-cell 95-105zinc-binding 95-104

Methylbixincharge-transfer complexes of 547-552

Microsomal membranesliver 475-480phenobarbital administration and 475-480phospholipids of 475-480

Microtubule proteinsPhysarum polycephalum 305-312

Microvillar membrane, see Brush-border membraneMilk

xanthine oxidase of 615-623Murex trunculus, see Sea-snailMuscle, skeletal, see Skeletal muscleMyelin basic protein

calmodulin and 227-240,455-459

Vol. 189

663

INDEX OF SUBJECTS

Neutral proteinaseuterus 521-531

Nitrite reductasenitrosyl- 285-294Pseudomonas aeruginosa

Nitrosyl-(nitrite reductase)Pseudomonas aeruginosa

Octopus (Octopus vulgaris)ribosomal ribonucleic acids of

Ovotransferrinchicken egg 541-546

285-294

285-294

313-335

Pancreasribonuclease A of 655-657

Papaya (Carica papaya)chymopapain A of 189-192

Penicilliumfuniculosumcellobiohydrolase of 51-65cellulose of 51-65

Phosphofructokinaseskeletal-muscle 561-567, 569-579

Phospholipidsendoplasmic-reticulum-membrane 475-480glycosphingolipids and 435-440microsomal-membrane 475-480

Physarum polycephalummicrotubule proteins of 305-312

Pituitary gland,i-endorphin of 501-506

Pituitary gland (anterior)prolactin of 605-614

Plasmafibronectin of 337-347

Polyadenylateribonucleases and 263-275, 277-284

Polyargininecalmodulin and 455-459

Polyenescharge-transfer complexes of 547-552conjugated 547-552

Polylysinecalmodulin and 455-459

Polyribosomescyclic nucleotide-independent protein kinases and

143-152cytoplasmic 143-152HeLa-cell 143-152nuclear 143-152

Polysomes, see PolyribosomesPolyspermine-ribonuclease

RNA-dependent deoxyribonucleic acid polymeraseand 89-93

Porphyrinsprotohaem ferro-lyase and 645-648

Prolactinanterior-pituitary-gland 605-614

Prolyl hydroxylasechick-embryo-tendon-fibroblast 491-499

Protaminecalmodulin and 455-459

Protease, see Proteinase

Proteiniron-sulphur, see Iron-sulphur proteinribosomal, see Ribosomal protein

Protein kinasescyclic nucleotide-independent 143-152HeLa-cell 143-152polyribosomes and 143-152

Proteinaseneutral, see Neutral proteinase

Proteinase inhibitortick (Boophilus microplus) 295-303

Proteinsdisulphide bonds of 507-520microtubule, see Microtubule proteins

Proteoglycancartilage 521-531neutral proteinase and 521-531

Protochlorophyllide reductasebarley-seedling 125-133etioplast 125-133light and 125-133wheat-seedling 125-133

Protohaem ferro-lyaseliver 645-648porphyrins and 645-648

Pseudomonas aeruginosanitrite reductase of 285-294

Pulse (Lathyrus sativus) seedlingsamidinotransferase of 553-560homoarginine metabolism in 553-560

Purple membraneHalobacterium halobium 413-420

Pyruvate dehydrogenase complexBacillus stearothermophilus 161-172

Pyruvate kinaseEscherichia coli 421-433

Rauscher leukaemia virusRNA-dependent deoxyribonucleic acid polymerase

of 89-93Red blood cells, see ErythrocytesReticulum, endoplasmic, see Endoplasmic reticulumReverse transcriptase, see Deoxyribonucleic acid poly-

merase, RNA-dependentRhodopseudomonas sphaeroides

cytochrome cl of 385-391Ribonuclease

Saccharomyces carlsbergensis 363-366Ribonuclease A,

disulphide bonds of 507-520pancreatic 655-657

Ribonuclease-polyspermineRNA-dependent deoxyribonucleic acid polymerase

and 89-93Ribonuclease S

disulphide bonds of 507-520Ribonucleases

polyadenylate and 263-275, 277-284skeletal-muscle 263-275, 277-284

Ribonucleic acidribosomal, see Ribosomal ribonucleic acid

Ribosomal protein S3BHK-cell 241-245

1980

664

INDEX OF SUBJECTS

Ribosomal ribonucleic acidsclam (Macrocallista chione) 313-335octopus (Octopus vulgaris) 313-335sea-snail (Murex trunculus) 3 13-335squid (Loligo vulgaris) 313-335

Saccharomyces carlsbergensisribonuclease of 363-366

Salivary glandkallikreins of 153-159

Scylliorhinus caniculus, see DogfishSea-snail (Murex trunculas)

ribosomal ribonucleic acids of 313-335Seedlings

amidinotransferase of 553-560homoarginine metabolism in 553-560pulse 553-560

Serine-pyruvate aminotransferaseliver 58 1-590

Serumtransferrin of 541-546

Serum albuminCibacron Blue F3G A and 27-34

Skeletal muscleadenylate deaminase of 367-368fructose bisphosphate aldolase of 17-25phosphofructokinase of 561-567, 569-579ribonucleases of 263-275, 277-284troponin T kinase of 219-226

Slime mould (Physarum polycephalum)microtubule proteins of 305-312

Small intestineindole 2,3-dioxygenase of 461-466

Spinach (Spinacia oleracea) leaveschloroplasts of 373-376fructose bisphosphatase of 373-376

Spinacia oleracea, see SpinachSpleen

indole 2,3-dioxygenase of 461-466Squid (Loligo vulgaris)

ribosomal ribonucleic acids of 313-335Starch granules

starch synthetase of 381-383sweet-potato 381-383

Starch synthetasestarch-granule 381-383sweet-potato 38 1-383

Stomachindole 2,3-dioxygenase of 461-466

Submandibular glandkallikreins of 153-159

Sweet potato (Ipomoea batatas)starch granules of 381-383starch synthetase of 381-383

Synoviumcollagenase of 349-357

Tamm-Horsfall glycoproteinurinary 533-539

Tendoncollagen of 111-124

Vol. 189

Tendon fibroblastschick-embryo 491-499prolyl hydroxylase of 491-499

Testisdeoxyribonucleic acid polymerases of 635-639dogfish 635-639

Thionein, see MetallothioneinThrombin

antithrombin III and 481-489Thromboplastin

brain 209-218protein component of 209-218

Tick (Boophilus microplus)proteinase inhibitor of 295-303

Transferrinovo-, see Ovotransferrinserum 54 1-546

Triticum sativum, see WheatTroponin T kinase

skeletal-muscle 219-226Trypsin, anhydro-, see AnhydrotrypsinTrypsinogen

disulphide bonds of 507-520Tryptophan 2,3-dioxygenase

liver 393-405Tryptophan pyrrolase, see Tryptophan 2,3-dioxygenase

Uridine diphosphate glucuronate-oestrone glucuronosyl-transferase

liver 377-380Uridine diphosphate glucuronate-testosterone glucurono-

syltransferaseliver 377-380

Uridine diphosphate glucuronic acidassay of 369-372

Urinearylsulphatase A of 45-49cerebroside sulphatase of 45-49Tamm-Horsfall glycoprotein of 533-539

Uterusneutral proteinase of 521-531

Vas deferensglutathione S-transferases of 135-142

Vicia graminealectin of 185-188

Virusinfluenza, see Influenza virusleukaemia, see Leukaemia virus

Wheat (Triticum sativum) seedlingsetioplasts of 125-133protochlorophyllide reductase of 125-133

Xanthine oxidasemilk 615-623

Yeast (Saccharomyces carlsbergensis)ribonuclease of 363-366

Zincmetallothionein binding of 95-104

665

THE

BIOCHEMICAL

JOURNALMOLECULAR ASPECTS

Volume 189

EDITORIAL BOARD

ChairmanJ. T. Dingle

Deputy ChairmenC. 1. PogsonA. J. KennyJ. B. ClarkA. J. Cornish-BowdenJ. A. Lucy

Editorial ManagerA. G. J. Evans

Editorial SecretaryA. S. Beedle

P. M. BayleyW. J. BrammarR. C. BrayJ. W. BridgesD. N. BrindleyH. G. BrittonK. BrocklehurstM. J. ClemensB. A. CookeD. D. DaviesR. T. DeanR. M. DentonF. M. DickinsonG. J. DuttonP. C. EngelJ. E. FothergillP. J. GarlickJ. L. GordonD. E. Griffiths

L. A. GrivellB. A. HaddockL. Hue0. T. G. JonesP. LundR. M. MarchbanksG. MurphyN. B. MyantK. B. M. ReidD. RobinsonD. SchulsterJ. E. ScottS. P. SpraggD. R. StanworthM. J. A. TannerP. TulkensR. T. Walker*Nominated by the BritishBiophysical Society

Overseas Advisory Panel

H. Beinert (U.S.A.), H. F. DeLuca (U.S.A.), W. Fiers (Belgium), R. W. Hanson (U.S.A.),0. Hayaishi (Japan), B. Hess (Germany), D. B. Keech (Australia), T. C. Laurent (Sweden),L. F. Leloir (Argentina), R. Ross (U.S.A.), M. Schramm (Israel), A. Tissi4res (Switzerland),D. Trentham (U.S.A.), A. D. Vinogradov (U.S.S.R.), 0. Wieland (Germany)

6)AMIC44 l

in^A

London: The Biochemical Society 1980

1980

THE BIOCHEMICAL SOCIETY, 7 WARWICK COURT, LONDON WC1R 5DP

ISSN: 0306-3275

Printed in Great Britain by Spottiswoode Ballantyne Ltd.Colchester and London