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Big Lake Aquatic Vegetation Management Plan 2009 Update Noble County, Indiana http://129.79.145.7/arcims/statewide%5Fmxd/viewer.htm Prepared for: The Big Lake Association 4878 South Pressler Drive Albion, IN 46701 March 1, 2010 Prepared By: Aquatic Weed Control P. O. Box 325 Syracuse, IN 46567

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Page 1: The Big Lake Association - IN.gov...The Big Lake Association 4878 South Pressler Drive Albion, IN 46701 March 1, 2010 Prepared By: Aquatic Weed Control P. O. Box 325 Syracuse, IN 46567

Big Lake Aquatic Vegetation Management Plan 2009 Update

Noble County, Indiana

http://129.79.145.7/arcims/statewide%5Fmxd/viewer.htm

Prepared for:

The Big Lake Association 4878 South Pressler Drive

Albion, IN 46701

March 1, 2010

Prepared By:

Aquatic Weed Control P. O. Box 325

Syracuse, IN 46567

Page 2: The Big Lake Association - IN.gov...The Big Lake Association 4878 South Pressler Drive Albion, IN 46701 March 1, 2010 Prepared By: Aquatic Weed Control P. O. Box 325 Syracuse, IN 46567

II

Executive Summary

This report describes aquatic vegetation management practices on Big Lake during its involvement in the IDNR’s Lake and River Enhancement Program (LARE). It is the 2009 update to the original aquatic vegetation management plan (AVMP) developed in 2006. Big Lake has received LARE funding to treat Eurasian watermilfoil (Myriophyllum spicatum) for the past three years and has been in the LARE program since 2006 when the first aquatic vegetation surveys were conducted. The current treatment strategy is designed to control EWM abundance in Big Lake while exploring the differences between spot treatments of Renovate and 2, 4-D herbicides. It is important to know how these herbicides affect not only the targeted invasive plants but non-target native plants as well. Renovate is more expensive than 2, 4-D and both are widely used in EWM spot treatments. As of 2009, Renovate as 2, 4-D both seem to control EWM adequately with similar effects on native species in Big Lake. This strategy is recommended for Big Lake in 2010. Using the same treatment strategy in 2010 will provide consistency through Big Lake’s four years of treatment funding priority while giving four years of data for the important comparison of Renovate and 2, 4-D herbicides. On June 16, 2009, 12 acres in basin 1 of Big Lake were treated with DMA-4 (2, 4-D) herbicide for selective root control of Eurasian watermilfoil (EWM). Twenty-two acres in basins 2 and 3 of Big Lake were treated with Renovate (triclopyr) herbicide for selective root control of Eurasian watermilfoil. Treatment areas were nearly identical to 2008. One additional acre in basin 1 of Big Lake was added for EWM treatment in 2009. Two Tier II aquatic vegetation surveys are conducted each year on Big Lake. In 2009, the first was conducted on June 12, 2009 (pre-treatment) and the second was conducted on July 30, 2009 (post-treatment). Eurasian watermilfoil was collected at 20.0% of all rake sample locations during the 2009 pre-treatment Tier II aquatic vegetation survey. The post treatment survey found that Eurasian watermilfoil was not collected in the post-treatment vegetation survey. This was a greater decline in EWM abundance than expected. Data from these surveys was also analyzed to study differences between basins and between herbicides in relationship to both invasive and native plant distribution. Detailed information of these results can be found in sections 8.2 and 8.3 of this report. The 2, 4-D and Renovate treatments conducted in 2007, 2008, and 2009 were successful at reducing Eurasian watermilfoil abundance, but it is very important for all parties to understand that although 2, 4-D and Renovate treatments provide very effective EWM control they only provide season long control. In 2010, Eurasian watermilfoil is expected to return to the 2009 treatment areas. Renovate and 2, 4-D cannot be expected to eradicate Eurasian watermilfoil in Big Lake. Maintenance of the Eurasian watermilfoil must be conducted on a yearly basis with this treatment program. Eurasian watermilfoil abundance is not expected to decline from year to year. The 2010 treatment strategy will be much the same as in 2008 and 2009. Up to 18 acres of EWM in Basin #1 will be treated with 2, 4-D. Up to 22 acres of EWM in Basins 2 and 3 will be treated with Renovate. Exact treatment areas will depend upon results from a pre-treatment Tier II survey in spring of 2010.

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Based on visual observation, Eurasian watermilfoil in some areas of Big Lake often starts to grow relatively late in spring. It is recommended that funding be reserved to treat any areas of EWM that might sprout after the initial treatments, provided these additional acreages do not bring the total 2010 treatment acreages above the initial permit acreages of 18 acres in Basin #1 and 22 acres in Basins #2 and #3. It is important to note that Eurasian watermilfoil will be the only plant species specifically targeted in this project, as LARE funds will be awarded only for the control of invasive plant species. The goal is not to eliminate vegetation in Big Lake but to improve the health of the plant community by reducing EWM abundance on an annual basis. Native vegetation will still be abundant in shallow areas after treatment, and control of these natives must be privately funded. The goal will be to provide yearly maintenance of the EWM population and allow for the recovery of native plant species that will provide better fish habitat, foster good water quality, and pose less interference to recreational use of the lake. A summary of 2010 management recommendations and project cost estimates are provided below.

2010 Proposed Budget Treat up to 18 acres in Basin #1 with 2, 4-D for Eurasian watermilfoil: up to $6,660 Treat up to 22 acres in Basins # 2 and #3 with Renovate for Eurasian watermilfoil: up to $10,780

2010 Surveys and Planning Two Tier II vegetation surveys are recommended for 2010. A spring pre-treatment survey and a late season post-treatment survey will take place. 2010 Tier II surveys and management plan update up to $6,300

2010 Total Cost Estimates Basin #1 treatment $6,660 Basin #2 and 3 Treatment $10,780 Tier II Surveys and plan update $6,300 Total maximum project costs $23,740 Lake and River Enhancement Share $21,366 Big Lake Association’s Share $2,374

Page 4: The Big Lake Association - IN.gov...The Big Lake Association 4878 South Pressler Drive Albion, IN 46701 March 1, 2010 Prepared By: Aquatic Weed Control P. O. Box 325 Syracuse, IN 46567

IV

Original 4 year budget estimates are included below for reference

Project 2008 2009 2010 2011 4 Year Cost

Totals Treat 18 acres in Basin #1 with 2, 4-D $6,480 $6,480 $6,480 $6,480 Treat 22 acres in Basins 2 and 3 with Renovate $10,450 $10,450 $10,450 $10,450

Plant Survey and Update Costs $6,000 $6,000 $6,000 $6,000 Total Estimated Costs $22,930 $22,930 $22,930 $22,930 $91,720 Total LARE share – subject to availability $20,637 $20,637 $20,637 $20,637 $82,548 Total Association’s Share $2,293 $2,293 $2,293 $2,293 $9,172

Page 5: The Big Lake Association - IN.gov...The Big Lake Association 4878 South Pressler Drive Albion, IN 46701 March 1, 2010 Prepared By: Aquatic Weed Control P. O. Box 325 Syracuse, IN 46567

V

Acknowledgements

Aquatic vegetation surveys conducted on Big Lake were made possible by funding from the Big Lake Association and the Indiana Department of Natural Resources through the Lake and River Enhancement program (LARE). Aquatic Weed Control would like to extend special thanks to Indiana Department of Natural Resources (IDNR) District 3 biologist Jed Pearson for providing procedural training for Tier II aquatic vegetation surveys. Angela Sturdevant, an aquatic biologist for the LARE program, provided valuable consultation regarding the requirements and objectives of this lake management plan. Aquatic Weed Control would also like to thank the members of the Big Lake Association for their commitment to improving this lake and for valuable discussion and input brought forward at the informational meeting held on August 29, 2009.

Page 6: The Big Lake Association - IN.gov...The Big Lake Association 4878 South Pressler Drive Albion, IN 46701 March 1, 2010 Prepared By: Aquatic Weed Control P. O. Box 325 Syracuse, IN 46567

VI

Table of Contents Acknowledgements............................................................................................................ V 

Table of Contents.............................................................................................................. VI 

List of Tables ................................................................................................................. VIII 

1.0 Introduction................................................................................................................... 9 

2.0 Watershed and Lake Characteristics........................................................................... 12 

3.0 Lake Uses Update ....................................................................................................... 12 

4.0 Fisheries Update.......................................................................................................... 12 

5.0 Problem Statement ...................................................................................................... 12 

6.0 Vegetation Management goals and Objectives........................................................... 13 

7.0 Past Management Efforts Update ............................................................................... 14 

8.0 Aquatic Plant Community Characterization Update................................................... 15 

8.1 Methods Update ............................................................................................................ 16 8.2.1 Tier II Results ............................................................................................................ 17 8.3 Macrophyte Inventory Discussion ................................................................................ 30 

9.0 Aquatic Plant Management Alternatives .................................................................... 37 

10.0 Public Involvement ................................................................................................... 37 

11.0 Public Education ....................................................................................................... 39 

12.0 Integrated Treatment Action Strategy....................................................................... 40 

13.0 Project Budget........................................................................................................... 41 

14.0 Monitoring and Plan Update Procedures .................................................................. 42 

15.0 References................................................................................................................. 43 

16.0 Appendices................................................................................................................ 43 

16.1 Calculations................................................................................................................. 43 16.2 Common Aquatic Plants of Indiana............................................................................ 46 16.3 Pesticide Use Restrictions Summary: ......................................................................... 46 16.4 Resources for Aquatic Management........................................................................... 47 16.5 State Regulations for Aquatic Plant Management ...................................................... 48 16.6 Species Distribution Maps .......................................................................................... 50 16.7 Data Sheets.................................................................................................................. 70 16.8 LARE Resume ............................................................................................................ 78 

Page 7: The Big Lake Association - IN.gov...The Big Lake Association 4878 South Pressler Drive Albion, IN 46701 March 1, 2010 Prepared By: Aquatic Weed Control P. O. Box 325 Syracuse, IN 46567

VII

16.9 IDNR Aquatic Vegetation Control Permit.................................................................. 81 

List of Figures

Figure 1: Big Lake 2009 EWM Treatment Areas................................................................... 14 Figure 2: Big Lake Spring 2009 Eurasian Watermilfoil Locations ........................................ 15 Figure 3: Big Lake Spring 2009 Curly Leaf Pondweed Locations......................................... 16 Figure 4: Big Lake Rake Sample Locations ........................................................................... 17 Figure 5: Big Lake Secchi Depth History............................................................................... 18 Figure 6: Big Lake Site Frequency Histories.......................................................................... 21 Figure 7: Big Lake Dominance Histories ............................................................................... 22 Figure 8: 2007 Basin 1 Site Frequencies ................................................................................ 27 Figure 9: 2008 Basin 1 Site Frequencies ................................................................................ 28 Figure 10: 2007 Basins 2 and 3 Site Frequencies ................................................................... 28 Figure 11: 2008 Basins 2 and 3 Site Frequencies ................................................................... 29 Figure 12: 2009 Basin 1 Site Frequencies .............................................................................. 29 Figure 13: 2009 Basins 2 and 3 Site Frequencies ................................................................... 30 Figure 14: Big Lake 2009 Treatment Areas ........................................................................... 31 Figure 15:Renovate Herbicide Effects on Coontail and EWM .............................................. 33 Figure 16: 2, 4-D Effects on Coontail and EWM ................................................................... 34 Figure 17: Big Lake 2009 Public Questionnaire..................................................................... 38 Figure 18: Big Lake Rake Sample Locations ......................................................................... 50 Figure 19: Big Lake Spring 2009 Elodea Locations............................................................... 51 Figure 20: Big Lake Spring 2009 Eel Grass Locations .......................................................... 52 Figure 21: Big Lake Spring 2009 Curly Leaf Pondweed Locations....................................... 53 Figure 22: Big Lake Spring 2009 Coontail Locations ............................................................ 54 Figure 23: Big Lake Spring 2009 Chara Locations ................................................................ 55 Figure 24: Big Lake Spring 2009 Small Pondweed Locations............................................... 56 Figure 25: Big Lake Spring 2009 Slender Naiad Locations ................................................... 57 Figure 26: Big Lake Spring 2009 Sago Pondweed Locations ................................................ 58 Figure 27: Big Lake Spring 2009 Large Leaf Pondweed Locations ...................................... 59 Figure 28: Big Lake Spring 2009 Flat-stemmed Pondweed Locations .................................. 60 Figure 29: Big Lake Spring 2009 Eurasian Watermilfoil Locations ...................................... 61 Figure 30: Big Lake Summer 2009 Eel Grass Locations ....................................................... 62 Figure 31: Big Lake Summer 2009 Coontail Locations ......................................................... 63 Figure 32: Big Lake Summer 2009 Chara Locations ............................................................. 64 Figure 33: Big Lake Summer 2009 Small Pondweed Locations............................................ 65 Figure 34: Big Lake Summer 2009 Slender Naiad Locations ................................................ 66 Figure 35: Big Lake Summer 2009 Sago Pondweed Locations ............................................. 67 Figure 36: Big Lake Summer 2009 Large Leaf Pondweed Locations.................................... 68 Figure 37: Big Lake Summer 2009 Elodea Locations............................................................ 69 Figure 38: Spring 2009 Data Cover Sheet .............................................................................. 70 Figure 39: Spring 2009 Data Sheet 1...................................................................................... 71 Figure 40: Spring 2009 Data Sheet 2...................................................................................... 72 Figure 41: Summer 2009 Data Cover Sheet ........................................................................... 73 Figure 42: Summer 2009 Data Sheet 1 ................................................................................... 74 Figure 43: Summer 2009 Data Sheet 2 ................................................................................... 75 

Page 8: The Big Lake Association - IN.gov...The Big Lake Association 4878 South Pressler Drive Albion, IN 46701 March 1, 2010 Prepared By: Aquatic Weed Control P. O. Box 325 Syracuse, IN 46567

VIII

List of Tables

Table 1: Big Lake LARE History ............................................................................................. 9 Table 2: Common and Scientific Plant Names ....................................................................... 10 Table 3: Big Lake Spring 2009 Data Analysis ....................................................................... 19 Table 4: Big Lake 2009 Late Season Data Analysis............................................................... 20 Table 5: Spring 2009 Basin #1 Data ....................................................................................... 23 Table 6: Summer 2009 Basin #1 Data .................................................................................... 24 Table 7: Spring 2009 Basins #2 and #3 Data.......................................................................... 25 Table 8: Summer 2009 Basins #2 and #3 Data....................................................................... 26 Table 9: AWC Eurasian Watermilfoil and Coontail Data ...................................................... 35 Table 10: IDNR Eurasian Watermilfoil and Coontail Data.................................................... 35 Table 11: Pesticide Use Restrictions....................................................................................... 46 Table 12: GPS Coordinates for Rake Sample Locations ........................................................ 76 

Page 9: The Big Lake Association - IN.gov...The Big Lake Association 4878 South Pressler Drive Albion, IN 46701 March 1, 2010 Prepared By: Aquatic Weed Control P. O. Box 325 Syracuse, IN 46567

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1.0 Introduction The first LARE funded aquatic vegetation survey conducted on Big Lake by Aquatic Weed Control took place on August 30, 2006. Another vegetation survey was conducted earlier in 2006 by District 3 Fisheries personnel on May 30th. Based on the results of these 2006 surveys, Eurasian watermilfoil treatments were recommended for 2007. Annual Eurasian watermilfoil treatments have taken place in 2007, 2008, and 2009. In 2007 a pre-treatment Tier II vegetation survey was conducted on May 17, 2007 to confirm Eurasian watermilfoil abundance and gather more pre-treatment data about the plant community. The LARE funded Eurasian watermilfoil herbicide treatments were conducted on June 7, 2007. Areas in Basin #1 were treated with Renovate and areas in Basins #2 and #3 were treated with 2, 4-D herbicide. A late season Tier II survey was conducted by Aquatic Weed Control on August 10, 2007 to evaluate the plant community. In 2008, 11 acres of Basin #1 were treated with 2, 4-D following the pre-treatment Tier II survey. Twenty two acres in Basins #2 and #3 were treated with Renovate. A late season Tier II survey was conducted on July 30, 2008. In 2009, 12 acres in Basin #1 were treated with 2, 4-D and 22 acres in Basins #2 and #3 were treated with Renovate herbicide. Table 1 summarizes LARE activities on Big Lake. The time frame for the original aquatic vegetation management plan (AVMP) runs through 2010. Table 1: Big Lake LARE History

Year Action Date Funding Source

2006

Spring Tier II Survey (IDNR) Late Season Tier II survey AVMP Update

May 30, 2006 August 30, 2006 Summer/Winter 2006

Lake and River Enhancement Program (LARE) Big Lake Association

2007

Spring Tier II Vegetation Survey LARE Funded 2, 4-D and Renovate Treatment for EWM (30 acres) Late Season Tier II Vegetation Survey

May 17, 2007 June 7, 2007 August 10, 2007

Lake and River Enhancement Program (LARE) Big Lake Association

2008

Spring Tier II Vegetation Survey LARE Funded 2, 4-D and Renovate Treatment for EWM (31 acres) Late Season Tier II Vegetation Survey

June 13, 2008 June 19, 2008 July 30, 2008

Lake and River Enhancement Program (LARE) Big Lake Association

2009

Spring Tier II Vegetation Survey LARE Funded 2, 4-D and Renovate Treatments (34 acres) Late Season Tier II Survey

June 12, 2009 June 16, 2009 July 30, 2009

Lake and River Enhancement Program (LARE) Big Lake Association

Page 10: The Big Lake Association - IN.gov...The Big Lake Association 4878 South Pressler Drive Albion, IN 46701 March 1, 2010 Prepared By: Aquatic Weed Control P. O. Box 325 Syracuse, IN 46567

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Table 2 was compiled by the IDNR and gives both common and scientific names of many plants mentioned in this report. It also gives species codes which may be referenced on some data sheets. Table 2: Common and Scientific Plant Names

Page 11: The Big Lake Association - IN.gov...The Big Lake Association 4878 South Pressler Drive Albion, IN 46701 March 1, 2010 Prepared By: Aquatic Weed Control P. O. Box 325 Syracuse, IN 46567

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Page 12: The Big Lake Association - IN.gov...The Big Lake Association 4878 South Pressler Drive Albion, IN 46701 March 1, 2010 Prepared By: Aquatic Weed Control P. O. Box 325 Syracuse, IN 46567

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2.0 Watershed and Lake Characteristics Secchi depth was measured at 5.0 feet on May 17, 2007 and at 4.1 feet on August 10, 2007. In 2008, Secchi depth was measured at 8.1 feet on June 14th and 6.9 feet on July 30th. In 2009, Secchi depth was measured at 9.1 feet on June 12th and 3.4 feet on July 30th. Algal blooms, along with rainsummer events, are likely factors that influence the fluctuation of water clarity in Big Lake which appears to fluctuate from spring to summer and from year to year. A new diagnostic study of seven lakes in the Upper Tippecanoe River Watershed is also now available. This project was completed by Williams Creek Consulting. It includes information on soils, land use, pollutants, and watershed management issues for this watershed which includes Big Lake. This project is an excellent resource for watershed information and can be viewed online through the IDNR’s Lake and River Enhancement website at the following address. ( http://www.in.gov/dnr/fishwild/3303.htm). 3.0 Lake Uses Update Lake uses on Big Lake are much the same as in 2006. They include boating, skiing, fishing, and nature observation in the undeveloped portions of the second and third basins. Big Lake is a popular lake for fishermen. Largemouth bass, bluegills, and yellow perch are all very popular sport fish and all are common in Big Lake. More information about the Big Lake fishery is included in section 4.0 in this report. Summer weekends can be very crowded on the lake with the public access site having limited parking space available. The lake also has a 10 mph speed limit, with high speed boating permitted in the first basin between 1 p.m. and 4 p.m. daily. 4.0 Fisheries Update District 3 Fisheries Biologist Jed Pearson was contacted for the latest fisheries data for Big Lake. No fisheries surveys took place on Big Lake during 2008. The most recent fisheries data can be found in the 2006 lake management plan. More surveys by the IDNR are planned for 2009 on Big Lake. 5.0 Problem Statement Eurasian watermilfoil will continue to be the major plant management challenge for 2010. Renovate and 2, 4-D treatments in 2007, 2008, and 2009 have provided very good seasonal control of EWM. However, it is important to note that although 2, 4-D and Renovate treatments provide very effective EWM control they only provide season long control. In 2010, Eurasian watermilfoil is expected to return to the 2009 treatment areas. 2, 4-D and Renovate cannot be expected to eradicate Eurasian watermilfoil in Big Lake. Eurasian watermilfoil abundance is not expected to decrease from year to year with these maintenance treatments. Maintenance treatments for Eurasian watermilfoil must be conducted on a yearly basis with the current treatment program.

Page 13: The Big Lake Association - IN.gov...The Big Lake Association 4878 South Pressler Drive Albion, IN 46701 March 1, 2010 Prepared By: Aquatic Weed Control P. O. Box 325 Syracuse, IN 46567

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Big Lake’s littoral zone (shallow water area) occupies a relatively small percentage of its total surface acreage (~17%). The large amount of deep water in the lake helps limit milfoil distribution, although it still causes significant recreational impairment in near shore areas around docks, piers, and beaches. The near shore areas should be the focus of management activities to improve recreation and reduce the Eurasian watermilfoil population. Selectively treating for Eurasian watermilfoil on a yearly basis should help native plants compete with the invasive plant. 6.0 Vegetation Management goals and Objectives The following management goals have been established by the IDNR for all lakes in Indiana, including those applying for LARE funding. Any management practices implemented on Big Lake are to directly facilitate the achievement of these three goals:

1. Develop or maintain a stable, diverse aquatic plant community that supports a good balance of predator and prey fish and wildlife species, good water quality and is resistant to minor habitat disturbances and invasive species.

2. Direct efforts to preventing and/or controlling the negative impacts of aquatic

invasive species.

3. Provide reasonable public recreational access while minimizing the negative impacts on plant and wildlife resources.

Specific Objectives: Specific objectives are needed to ensure that the fundamental goals of the LARE program are met. One specific measurable objective in Big Lake would be to maintain Eurasian watermilfoil site frequency at or below 25% in yearly vegetation surveys. This is not a performance guarantee, as Aquatic Weed Control still recommends Sonar treatments as the most effective and cost effective treatment for Eurasian watermilfoil. However, a 25% frequency limit would indicate whether or not the current management strategy is stopping the spread of Eurasian watermilfoil. The following steps are recommended to help achieve LARE management goals for Big Lake.

1. Areas infested with Eurasian watermilfoil in basin #1 will be treated with 2, 4-D to reduce the Eurasian watermilfoil population in 2010. Exact treatment areas will depend upon results of a spring 2010 visual survey. Treatment areas will likely be very similar to 2008 and 2009.

2. Areas infested with Eurasian watermilfoil in basins #2 and #3 will be treated

with Renovate. Again, exact treatment areas will depend upon results of a spring 2010 visual survey. Renovate treatments will protect native coontail in these areas.

3. Vegetation surveys should be conducted to evaluate the plant community both

before and after treatment in 2010. A spring Tier II survey should be conducted prior to herbicide treatments so that differences between 2, 4-D and Renovate treatments can be studied further. A Tier II vegetation survey should be conducted after the chemical treatment to evaluate the plant community.

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7.0 Past Management Efforts Update

District 3 Fisheries Biologist Jed Pearson was contacted to determine any major changes to vegetation control permits on Big Lake. The only significant changes in 2009 were the LARE funded herbicide treatments.

Private treatments for coontail could be permitted in 2010 but would be considered by the IDNR on a case by case basis. Jed Pearson, District 3 Fisheries Biologist, may inspect areas of concern in 2010 to determine if they may be eligible for an aquatic vegetation control permit.

On June 7, 2007 the first LARE funded herbicide treatment was conducted on Big Lake. Infested areas in basin #1 of Big Lake were treated with Renovate for the control of Eurasian watermilfoil. In basins #2 and #3, 2, 4-D was used to control Eurasian watermilfoil.

On June 19, 2008, 11 acres in Basin #1 of Big Lake were treated with 2, 4-D. Twenty-two acres in Basins # 2 and #3 were treated with Renovate. Eurasian watermilfoil was the target species for both of these treatments.

On June 16, 2009, 12 acres in basin #1 of Big Lake were treated with DMA-4 (2, 4-D) herbicide for selective root control of Eurasian watermilfoil. Twenty-two acres in basins #2 and basin #3 of Big Lake were treated with Renovate (triclopyr) herbicide for selective root control of Eurasian watermilfoil. Figure 1 shows locations, acreages, and herbicide use for the 2009 LARE funded Eurasian watermilfoil treatments on Big Lake. Figure 1: Big Lake 2009 EWM Treatment Areas

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8.0 Aquatic Plant Community Characterization Update Survey methods and data analysis remain unchanged since the 2007 AVMP update. Eurasian watermilfoil and curly leaf pondweed were the two invasive plant species collected in Big Lake in 2009. Each of these species was collected in spring of 2009 but not in the late season survey in 2009. Figure 2 shows rake sample locations where Eurasian watermilfoil was collected in the June 12, 2009 survey prior to treatment. Figure 3 shows curly leaf pondweed locations on June 12, 2009. Figure 2: Big Lake Spring 2009 Eurasian Watermilfoil Locations

Page 16: The Big Lake Association - IN.gov...The Big Lake Association 4878 South Pressler Drive Albion, IN 46701 March 1, 2010 Prepared By: Aquatic Weed Control P. O. Box 325 Syracuse, IN 46567

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Figure 3: Big Lake Spring 2009 Curly Leaf Pondweed Locations

8.1 Methods Update The Tier II survey protocol was updated by the IDNR in 2006 and 2007. The 2006 Tier II protocol requires that sample sites be stratified by depth contour and that data analysis be provided for each depth contour. Rake scores for plant species are recorded as 1, 3, or 5, as opposed to the original scoring system of 1, 2, 3, 4, or 5. Big Lake is classified by the IDNR as eutrophic with 228 surface acres. Based on these characteristics, 60 sample sites are distributed throughout each 5 foot depth contour of the littoral zone. Maximum sampling depth was 15 feet. Thirty samples were collected in the 0 – 5 foot depth contour. Twenty samples were collected in the 5 – 10 foot depth contour, and 10 samples were collected in the 10 -15 foot depth contour. Based on recent survey data it is recommended that survey protocol and maximum sampling depth remain the same for Big Lake. Although plants are not abundant in depths of over 9 feet, it may be possible to find plants (especially coontail) in greater depths.

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8.2.1 Tier II Results Two Tier II aquatic vegetation surveys were conducted by Aquatic Weed Control on Big Lake in 2009. The first was conducted on June 12, 2009, and the second was conducted on July 30, 2009. Secchi depth was measured at 9.1 feet on June 12th and at 3.4 feet on July 30th. In each survey, sixty rake samples were distributed throughout the lake. A total of 11 species of submersed aquatic plants were collected in spring, while 8 species were collected in the late season survey. Two invasive species (Eurasian milfoil and curly-leaf pondweed) were found in spring but not in the late season survey. Rake sample locations have remained unchanged since 2006. Figure 4 shows these rake sample locations. Maximum sampling depth was 15 feet. Thirty samples were collected in the 0 – 5 foot depth contour. Twenty samples were collected in the 5 – 10 foot depth contour, and 10 samples were collected in the 10 -15 foot depth contour. Figure 4: Big Lake Rake Sample Locations

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Secchi Depth Secchi Depth in Big Lake appears to have increased somewhat over the past 4 years, but it is important to note that Secchi depth can change quickly with rain events, algal blooms, and other factors. Secchi can continue to be tracked to see if this pattern continues. Figure 5: Big Lake Secchi Depth History

Table 3 and Table 4 are data summaries for the 2009 aquatic vegetation surveys on Big Lake. These surveys help to describe the plant community and will help identify any changes that take place in the years to come. Sections labeled “Overall” analyze every sample site, while the others describe the plants in each depth contour of the lake (0-5 feet, 5-10 feet, etc). In the data analysis tables, “littoral sites”, indicates the number of sample sites which had a depth that was less than the maximum depth at which plants were found. The littoral depth indicates the maximum depth at which plants were found.

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Table 3: Big Lake Spring 2009 Data Analysis

County: Noble Total Sites: 60 Mean species/site: 1.92Date: 6/12/2009 Sites with plants: 44 SE Mean species/site: 0.19

Secchi (ft): 9.1 Sites with native plants: 44 Mean native species/site: 1.50Maximum Plant Depth (ft): 12.5 Number of species: 11 SE Mean natives/site: 0.15

Trophic Status: Eutrophic Number of native species: 9 Species diversity: 0.83Maximum species/site: 5 Native species diversity: 0.77

All Depths (0 to 12.5 ft)Species 0 1 3 5Coontail 61.7 38.3 18.3 26.7 16.7 36.3Eelgrass 26.7 73.3 18.3 8.3 0.0 8.7Curly-Leaf Pondweed 21.7 78.3 13.3 8.3 0.0 7.7Eurasian Watermilfoil 20.0 80.0 13.3 6.7 0.0 6.7Small Pondweed 18.3 81.7 18.3 0.0 0.0 3.7Chara 11.7 88.3 1.7 5.0 5.0 8.3Large-Leaf Pondweed 11.7 88.3 3.3 1.7 6.7 8.3Slender Naiad 8.3 91.7 6.7 1.7 0.0 2.3Elodea 6.7 93.3 6.7 0.0 0.0 1.3Sago Pondweed 3.3 96.7 3.3 0.0 0.0 0.7Flat-Stemmed Pondweed 1.7 98.3 0.0 1.7 0.0 1.0

Filamentous Algae 30.0Depth: 0 to 5 ftSpecies 0 1 3 5Coontail 80.0 20.0 20.0 33.3 26.7 50.7Eelgrass 46.7 53.3 33.3 13.3 0.0 14.7Curly-Leaf Pondweed 33.3 66.7 20.0 13.3 0.0 12.0Eurasian Watermilfoil 33.3 66.7 20.0 13.3 0.0 12.0Chara 23.3 76.7 3.3 10.0 10.0 16.7Large-Leaf Pondweed 20.0 80.0 6.7 3.3 10.0 13.3Small Pondweed 16.7 83.3 16.7 0.0 0.0 3.3Elodea 10.0 90.0 10.0 0.0 0.0 2.0Slender Naiad 10.0 90.0 6.7 3.3 0.0 3.3Flat-Stemmed Pondweed 3.3 96.7 0.0 3.3 0.0 2.0Sago Pondweed 3.3 96.7 3.3 0.0 0.0 0.7

Filamentous Algae 43.3Depth: 5 to 10 ftSpecies 0 1 3 5Coontail 55.0 45.0 20.0 25.0 10.0 29.0Small Pondweed 25.0 75.0 25.0 0.0 0.0 5.0Curly-Leaf Pondweed 10.0 90.0 5.0 5.0 0.0 4.0Eelgrass 10.0 90.0 5.0 5.0 0.0 4.0Eurasian Watermilfoil 10.0 90.0 10.0 0.0 0.0 2.0Slender Naiad 10.0 90.0 10.0 0.0 0.0 2.0Elodea 5.0 95.0 5.0 0.0 0.0 1.0Large-Leaf Pondweed 5.0 95.0 0.0 0.0 5.0 5.0Sago Pondweed 5.0 95.0 5.0 0.0 0.0 1.0

Filamentous Algae 25Depth: 10 to 15 ftSpecies 0 1 3 5Coontail 20.0 80.0 10.0 10.0 0.0 8.0Curly-Leaf Pondweed 10.0 90.0 10.0 0.0 0.0 2.0Small Pondweed 10.0 90.0 10.0 0.0 0.0 2.0

Filamentous Algae 0.0

Rake score frequency per species

Occurrence and Abundance of Submersed Aquatic Plants in Big Lake

Plant Dominance

Rake score frequency per species Plant Dominance

Frequency of Occurrence

Frequency of Occurrence

Frequency of Occurrence

Rake score frequency per species Plant Dominance

Frequency of Occurrence

Rake score frequency per species Plant Dominance

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Table 4: Big Lake 2009 Late Season Data Analysis

County: Noble Total Sites: 60 Mean species/site: 1.70Date: 7/30/2009 Sites with plants: 43 SE Mean species/site: 0.19

Secchi (ft): 3.4 Sites with native plants: 43 Mean native species/site: 1.70Maximum Plant Depth (ft): 9.5 Number of species: 8 SE Mean natives/site: 0.19

Trophic Status: Eutrophic Number of native species: 8 Species diversity: 0.75Maximum species/site: 5 Native species diversity: 0.75

All Depths (0 to 9.5 ft)Species 0 1 3 5Coontail 65.0 35.0 20.0 13.3 31.7 43.7Eelgrass 46.7 53.3 5.0 20.0 21.7 34.7Slender Naiad 26.7 73.3 10.0 16.7 0.0 12.0Largeleaf Pondweed 11.7 88.3 1.7 6.7 3.3 7.7Elodea 6.7 93.3 1.7 5.0 0.0 3.3Chara 5.0 95.0 3.3 0.0 1.7 2.3Small Pondweed 5.0 95.0 3.3 1.7 0.0 1.7Sago Pondweed 3.3 96.7 1.7 1.7 0.0 1.3

Filamentous Algae 20.0Depth: 0 to 5 ftSpecies 0 1 3 5Coontail 90.0 10.0 20.0 13.3 56.7 68.7Eelgrass 76.7 23.3 6.7 33.3 36.7 58.0Slender Naiad 46.7 53.3 13.3 33.3 0.0 22.7Largeleaf Pondweed 23.3 76.7 3.3 13.3 6.7 15.3Elodea 13.3 86.7 3.3 10.0 0.0 6.7Small Pondweed 10.0 90.0 6.7 3.3 0.0 3.3Chara 6.7 93.3 6.7 0.0 0.0 1.3Sago Pondweed 6.7 93.3 3.3 3.3 0.0 2.7

Filamentous Algae 36.7Depth: 5 to 10 ftSpecies 0 1 3 5Coontail 60.0 40.0 30.0 20.0 10.0 28.0Eelgrass 25.0 75.0 5.0 10.0 10.0 17.0Slender Naiad 10.0 90.0 10.0 0.0 0.0 2.0Chara 5.0 95.0 0.0 0.0 5.0 5.0

Filamentous Algae 5.0No plants were found in depths greater than 9.5 feet

Rake score frequency per species

Occurrence and Abundance of Submersed Aquatic Plants in Big Lake

Plant Dominance

Rake score frequency per species Plant Dominance

Frequency of Occurrence

Frequency of Occurrence

Frequency of Occurrence

Rake score frequency per species Plant Dominance

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Site Frequency Site frequency is a measure of how often a species was collected during the Tier II survey. It can be calculated by the following equation:

Site Frequency = (# of sites where the species was collected) X 100 Total # of littoral sample sites

Figure 6 shows site frequency histories for each plant collected by Aquatic Weed Control in any of the late season Tier II surveys over the past 4 years. Coontail is the most common plant in Big Lake with late season site frequencies always above 40%. Eel grass is not as abundant in spring, but is normally the second most abundant plant in the late season surveys. It is especially abundant in the north end of Basin #1 and the east end of Basin #2. Small Pondweed was frequently collected in 2007 and 2008, but its frequency dropped in 2009. Large leaf pondweed site frequency doubled from 2008 to 2009. Large leaf pondweed can be very beneficial to fish populations. Eurasian watermilfoil was not found in the 2009 late season surveys, which was not expected. It is hoped that EWM abundance will remain very low in future years in which LARE funded treatments are conducted. Figure 6: Big Lake Site Frequency Histories

Species Summer 2006 Summer 2007 Summer 2008 Summer 2009Coontail 60.0 46.7 46.7 65.0

Eel Grass 40.0 30.0 48.3 46.7

Slender Naiad 18.3 21.7 30.0 26.7

Eurasian milfoil 11.7 1.7 3.3 0.0

Chara 5.0 8.3 13.3 5.0

Sago Pondweed 5.0 3.3 0.0 3.3

Illinois P.W. 5.0 1.7 0.0 0.0

Curly‐leaf P.W. 3.3 10.0 8.3 0.0

Elodea 1.7 0.0 1.7 6.7

Large‐leaf P.W. 1.7 1.7 5.0 11.7

Small Pondweed 1.7 20.0 36.7 5.0

Richardsons P.W. 1.7 0.0 0.0 0.0

Flat‐stemmed P.W. 0.0 3.3 0.0 0.0

American pondweed 0.0 0.0 1.7 0.0 Species Diversity The species diversity indices listed in data analysis tables help to describe the overall plant community. A species diversity index is actually measured as a value of uncertainty (H). If a species is chosen at random from a collection containing a certain number of species, the diversity index (H) is the probability that a chosen species will be different from the previous random selection. The diversity index (H) will always be between 0 and 1. The higher the H value, the more likely it is that the next species chosen from the collection at random will be different from the previous selection (Smith, 2001). This index is dependent upon species

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richness and species evenness, meaning that species diversity is a function of how many different species are present and how evenly they are spread throughout the ecosystem. The species diversity index for Big Lake in the June 2009 survey was 0.83, which is nearly identical to the 2008 value of 0.84. These values are up from 0.72 in spring of 2007. Diversity in the July 2009 survey was 0.75 which is down from 0.81 in both 2007 and 2008. Native plant diversity in the June 2008 survey was measured at 0.77 which is identical to 2008. These values are up from 0.48 in spring of 2007. Native diversity in summer of 2009 was 0.75 which dropped slightly from 0.79 in the July 2008 survey and 0.78 in 2007 Species Dominance Species dominance is dependent upon how many times a species occurs and its relative coverage area or biomass within the system. In this survey, the abundance rating given to each species at each sample site was used to determine dominance. The dominance of a particular species in this Tier II survey increases as its site frequency and relative abundance increase. Figure 7 shows dominance values for each plant collected in late season Tier II surveys between 2006 and 2009. Trends are similar to site frequency with coontail being the most dominant plant in Big Lake. Eel grass dominance is second, followed by slender naiad. Large leaf pondweed dominance increased from 1.7 in 2008 to 7.7 in 2009. The two invasive plants in Big Lake (curly leaf and Eurasian watermilfoil) were not collected in the late season survey in 2009. Figure 7: Big Lake Dominance Histories

Species Summer 2006 Summer 2007 Summer 2008 Summer 2009Coontail 30.7 26.7 26.7 43.7

Eel Grass 20.7 13.3 25.7 34.7

Slender Naiad 4.3 6.3 17.3 12.0

Small Pondweed 0.3 6.7 9.3 1.7

Curly‐leaf P.W. 0.7 2.7 1.7 0.0

Chara 3.0 4.3 7.3 2.3

Flat‐stemmed P.W. 0.0 0.7 0.0 0.0

Sago Pondweed 1.7 0.7 0.0 1.3

Eurasian milfoil 3.0 0.3 0.7 0.0

Illinois P.W. 1.0 0.3 0.3 0.0

Large‐leaf P.W. 0.3 0.3 1.7 7.7

Elodea 1.0 0.0 0.3 3.3

Richardsons P.W. 0.3 0.0 0.0 0.0

American pondweed 0.0 0.0 0.3 0.0 Basin #1 vs. Basins #2 and #3 One of the major goals of the Big Lake treatment strategy is to compare Renovate and 2, 4-D treatments to determine what different effects each herbicide may have on both Eurasian watermilfoil and native plant populations. For this reason, data collected during the 2007 2008, and 2009 Tier II surveys was sorted according to treatment areas. In 2007, portions of Basin #1 were treated with Renovate while portions of Basins #2 and #3 were treated with 2,

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4-D in 2007. In 2008 and 2009 Basin #1 was treated with 2, 4-D and Renovate was used in Basins #2 and #3. For this reason, data from sample locations in Basin #1 was separated from sample locations in Basins #2 and #3. It is important to note the limitations of this comparison. Only portions of each basin were treated, in accordance with areas of high Eurasian watermilfoil abundance (see Figure 1 For this reason, there are many rake samples outside of the treatment areas which may also have an effect on this data. Also natural life cycles of many plants in Big Lake (curly leaf, eel grass, slender naiad etc.) may make it more challenging to determine the effects that herbicide treatments are having on some species. Still it is valuable to compare the different basins of Big Lake to document any potential changes in the plant community. The following analysis tables separate data from each basin for the 2009 Tier II surveys.

Basin #1: 2008 Spring and Summer Data

Table 5: Spring 2009 Basin #1 Data

County: Noble Total Sites: 32 Mean species/site: 1.69Date: 6/12/2009 Sites with plants: 22 SE Mean species/site: 0.26

Secchi (ft): 9.1 Sites with native plants: 22 Mean native species/site: 1.44Maximum Plant Depth (ft): 10.0 Number of species: 9 SE Mean natives/site: 0.22

Trophic Status: Eutrophic Number of native species: 7 Species diversity: 0.82Maximum species/site: 5 Native species diversity: 0.77

All DepthsSpecies 0 1 3 5Coontail 53.1 46.9 21.9 28.1 3.1 24.4Eelgrass 37.5 62.5 25.0 12.5 0.0 12.5Eurasian Watermilfoil 15.6 84.4 9.4 6.3 0.0 5.6Large-Leaf Pondweed 15.6 84.4 6.3 3.1 6.3 9.4Small Pondweed 12.5 87.5 12.5 0.0 0.0 2.5Chara 9.4 90.6 0.0 6.3 3.1 6.9Curly-Leaf Pondweed 9.4 90.6 6.3 3.1 0.0 3.1Elodea 9.4 90.6 9.4 0.0 0.0 1.9Slender Naiad 6.3 93.8 3.1 3.1 0.0 2.5

Filamentous Algae 40.6Depth: 0 to 5 ftSpecies 0.0 1.0 3.0 5.0Coontail 68.8 31.3 31.3 31.3 6.3 31.3Eelgrass 62.5 37.5 43.8 18.8 0.0 20.0Eurasian Watermilfoil 31.3 68.8 18.8 12.5 0.0 11.3Large-Leaf Pondweed 31.3 68.8 12.5 6.3 12.5 18.8Chara 18.8 81.3 0.0 12.5 6.3 13.8Curly-Leaf Pondweed 12.5 87.5 12.5 0.0 0.0 2.5Elodea 12.5 87.5 12.5 0.0 0.0 2.5Small Pondweed 12.5 87.5 12.5 0.0 0.0 2.5Slender Naiad 6.3 93.8 0.0 6.3 0.0 3.8

Filamentous Algae 56.3Depth: 5 to 10 ftSpecies 0.0 1.0 3.0 5.0Coontail 54.5 45.5 18.2 36.4 0.0 25.5Eelgrass 18.2 81.8 9.1 9.1 0.0 7.3Small Pondweed 18.2 81.8 18.2 0.0 0.0 3.6Curly-Leaf Pondweed 9.1 90.9 0.0 9.1 0.0 5.5Elodea 9.1 90.9 9.1 0.0 0.0 1.8Slender Naiad 9.1 90.9 9.1 0.0 0.0 1.8

Filamentous Algae 36.4

Frequency of Occurrence

Rake score frequency per species Plant Dominance

No plants were found deeper than 10 feet

Rake score frequency per species

Occurrence and Abundance of Submersed Aquatic Plants in Big Lake - Basin 1

Plant Dominance

Rake score frequency per species Plant Dominance

Frequency of Occurrence

Frequency of Occurrence

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Table 6: Summer 2009 Basin #1 Data

County: Noble Total Sites: 32 Mean species/site: 1.91Date: 7/30/2009 Sites with plants: 23 SE Mean species/site: 0.28

Secchi (ft): 3.4 Sites with native plants: 23 Mean native species/site: 1.91Maximum Plant Depth (ft): 9.5 Number of species: 7 SE Mean natives/site: 0.28

Trophic Status: Eutrophic Number of native species: 7 Species diversity: 0.75Maximum species/site: 5 Native species diversity: 0.75

All Depths Species 0 1 3 5Coontail 65.6 34.4 25.0 12.5 28.1 40.6Eelgrass 59.4 40.6 3.1 28.1 28.1 45.6Slender Naiad 31.3 68.8 9.4 21.9 0.0 15.0Largeleaf Pondweed 12.5 87.5 0.0 9.4 3.1 8.8Elodea 9.4 90.6 3.1 6.3 0.0 4.4Sago Pondweed 6.3 93.8 3.1 3.1 0.0 2.5Small Pondweed 6.3 93.8 6.3 0.0 0.0 1.3

Filamentous Algae 18.8Depth: 0 to 5 ftSpecies 0.0 1.0 3.0 5.0Eelgrass 93.8 6.3 0.0 43.8 50.0 76.3Coontail 87.5 12.5 25.0 12.5 50.0 62.5Slender Naiad 62.5 37.5 18.8 43.8 0.0 30.0Largeleaf Pondweed 25.0 75.0 0.0 18.8 6.3 17.5Elodea 18.8 81.3 6.3 12.5 0.0 8.8Sago Pondweed 12.5 87.5 6.3 6.3 0.0 5.0Small Pondweed 12.5 87.5 12.5 0.0 0.0 2.5

Filamentous Algae 37.5Depth: 5 to 10 ftSpecies 0.0 1.0 3.0 5.0Coontail 63.6 36.4 36.4 18.2 9.1 27.3Eelgrass 36.4 63.6 9.1 18.2 9.1 21.8

Filamentous Algae 0.0

Occurrence and Abundance of Submersed Aquatic Plants in Big Lake -Basin 1

Plant Dominance

Rake score frequency per species Plant Dominance

Frequency of Occurrence

Frequency of Occurrence

Frequency of Occurrence

Rake score frequency per species Plant Dominance

Rake score frequency per species

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Basins #2 and #3: 2008 Spring and Summer Data Table 7: Spring 2009 Basins #2 and #3 Data

County: Noble Total Sites: 28 Mean species/site: 2.18Date: 6/12/2009 Sites with plants: 22 SE Mean species/site: 0.28

Secchi (ft): 9.1 Sites with native plants: 22 Mean native species/site: 1.57Maximum Plant Depth (ft): 12.5 Number of species: 11 SE Mean natives/site: 0.21

Trophic Status: Eutrophic Number of native species: 9 Species diversity: 0.83Maximum species/site: 5 Native species diversity: 0.74

All Depths Species 0.0 1.0 3.0 5.0Coontail 71.4 28.6 14.3 25.0 32.1 50.0Curly-Leaf Pondweed 35.7 64.3 21.4 14.3 0.0 12.9Eurasian Watermilfoil 25.0 75.0 17.9 7.1 0.0 7.9Small Pondweed 25.0 75.0 25.0 0.0 0.0 5.0Chara 14.3 85.7 3.6 3.6 7.1 10.0Eelgrass 14.3 85.7 10.7 3.6 0.0 4.3Slender Naiad 10.7 89.3 10.7 0.0 0.0 2.1Large-Leaf Pondweed 7.1 92.9 0.0 0.0 7.1 7.1Sago Pondweed 7.1 92.9 7.1 0.0 0.0 1.4Elodea 3.6 96.4 3.6 0.0 0.0 0.7Flat-Stemmed Pondweed 3.6 96.4 0.0 3.6 0.0 2.1

Filamentous Algae 17.9Depth: 0 to 5 ftSpecies 0.0 1.0 3.0 5.0Coontail 92.9 7.1 7.1 35.7 50.0 72.9Curly-Leaf Pondweed 57.1 42.9 28.6 28.6 0.0 22.9Eurasian Watermilfoil 35.7 64.3 21.4 14.3 0.0 12.9Chara 28.6 71.4 7.1 7.1 14.3 20.0Eelgrass 28.6 71.4 21.4 7.1 0.0 8.6Small Pondweed 21.4 78.6 21.4 0.0 0.0 4.3Slender Naiad 14.3 85.7 14.3 0.0 0.0 2.9Elodea 7.1 92.9 7.1 0.0 0.0 1.4Flat-Stemmed Pondweed 7.1 92.9 0.0 7.1 0.0 4.3Large-Leaf Pondweed 7.1 92.9 0.0 0.0 7.1 7.1Sago Pondweed 7.1 92.9 7.1 0.0 0.0 1.4

Filamentous Algae 28.6Depth: 5 to 10 ftSpecies 0.0 1.0 3.0 5.0Coontail 55.6 44.4 22.2 11.1 22.2 33.3Small Pondweed 33.3 66.7 33.3 0.0 0.0 6.7Eurasian Watermilfoil 22.2 77.8 22.2 0.0 0.0 4.4Curly-Leaf Pondweed 11.1 88.9 11.1 0.0 0.0 2.2Large-Leaf Pondweed 11.1 88.9 0.0 0.0 11.1 11.1Sago Pondweed 11.1 88.9 11.1 0.0 0.0 2.2Slender Naiad 11.1 88.9 11.1 0.0 0.0 2.2

Filamentous Algae 11.1Depth: 10 to 15 ftSpecies 0.0 1.0 3.0 5.0Coontail 40.0 60.0 20.0 20.0 0.0 16.0Curly-Leaf Pondweed 20.0 80.0 20.0 0.0 0.0 4.0Small Pondweed 20.0 80.0 20.0 0.0 0.0 4.0

Filamentous Algae 0.0

Occurrence and Abundance of Submersed Aquatic Plants in Big Lake Basins 2-3

Plant Dominance

Rake score frequency per species Plant Dominance

Frequency of Occurrence

Frequency of Occurrence

Frequency of Occurrence

Rake score frequency per species Plant Dominance

Frequency of Occurrence

Rake score frequency per species Plant Dominance

Rake score frequency per species

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Table 8: Summer 2009 Basins #2 and #3 Data

County: Noble Total Sites: 28 Mean species/site: 1.46Date: 7/30/2009 Sites with plants: 20 SE Mean species/site: 0.24

Secchi (ft): 3.4 Sites with native plants: 20 Mean native species/site: 1.46Maximum Plant Depth (ft): 9.0 Number of species: 7 SE Mean natives/site: 0.24

Trophic Status: Eutrophic Number of native species: 7 Species diversity: 0.73Maximum species/site: 4 Native species diversity: 0.73

All DepthsSpecies 0.0 1.0 3.0 5.0Coontail 64.3 35.7 14.3 14.3 35.7 47.1Eelgrass 32.1 67.9 7.1 10.7 14.3 22.1Slender Naiad 21.4 78.6 10.7 10.7 0.0 8.6Chara 10.7 89.3 7.1 0.0 3.6 5.0Largeleaf Pondweed 10.7 89.3 3.6 3.6 3.6 6.4Elodea 3.6 96.4 0.0 3.6 0.0 2.1Small Pondweed 3.6 96.4 0.0 3.6 0.0 2.1

Filamentous Algae 21.4Depth: 0 to 5 ftSpecies 0.0 1.0 3.0 5.0Coontail 92.9 7.1 14.3 14.3 64.3 75.7Eelgrass 57.1 42.9 14.3 21.4 21.4 37.1Slender Naiad 28.6 71.4 7.1 21.4 0.0 14.3Largeleaf Pondweed 21.4 78.6 7.1 7.1 7.1 12.9Chara 14.3 85.7 14.3 0.0 0.0 2.9Elodea 7.1 92.9 0.0 7.1 0.0 4.3Small Pondweed 7.1 92.9 0.0 7.1 0.0 4.3

Filamentous Algae 35.7Depth: 5 to 10 ftSpecies 0.0 1.0 3.0 5.0Coontail 55.6 44.4 22.2 22.2 11.1 28.9Slender Naiad 22.2 77.8 22.2 0.0 0.0 4.4Chara 11.1 88.9 0.0 0.0 11.1 11.1Eelgrass 11.1 88.9 0.0 0.0 11.1 11.1

Filamentous Algae 11.1

Occurrence and Abundance of Submersed Aquatic Plants in Big Lake - Basins 2-3

Plant Dominance

Rake score frequency per species Plant Dominance

Frequency of Occurrence

Frequency of Occurrence

Frequency of Occurrence

Rake score frequency per species Plant Dominance

Rake score frequency per species

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Data by Treatment Area The following graphs describe pre and post- treatment data for years 2007, 2008, and 2009. These graphs are included to help identify possible effects Renovate and 2, 4-D might have on both native and invasive plants in Big Lake. Constructing this comparison was one of the major reasons for implementing this treatment strategy on Big Lake. Figure 8 and Figure 9 show site frequencies for plants collected in Basin #1 in both 2007 and 2008. Infested areas of Basin #1 were treated with Renovate on June 7, 2007 (between the two surveys). In 2008, infested areas of Basin #1 were treated with 2, 4-D. These two graphs offer a a before and after picture of both the Renovate treatment in 2007 and the 2, 4-D treatment in 2008. Coontail, the most prevalent native plant in Big Lake showed a slight decline in site frequency in both 2007 and 2008. Eurasian watermilfoil showed a decline in both 2007 and 2008 as well. It is important to note that increases in some species like eel grass and slender naiad are most likely more related to the life cycle of the plant than the herbicide treatments. Figure 8: 2007 Basin 1 Site Frequencies

Big Lake Basin 1 2007 Site Frequencies (Partially Treated with Renovate)

43.8

21.915.6

12.56.3 6.3

3.1 0.0 0.00 0 0 0 06.3

25.031.3

12.5

3.10.03.1

37.5

0.05.0

10.015.020.025.030.035.040.045.050.0

Coonta

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Curly-

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Elode

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n m

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Larg

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.

Flat-S

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weed

Chara

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Eel G

rass

Small

Pon

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Sago

Pondw

eed

May-07 Aug-07

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Figure 9: 2008 Basin 1 Site Frequencies

Big Lake Basin 1 2008 Site Frequencies (Partially Treated with 2, 4-D

5043.8

28.1

15.69.4 9.4 9.4 6.3 6.3

0

46.9

65.6

0 3.1 3.1

43.8

9.43.1

40.6

3.1

010203040506070

Coonta

il

Eel Gra

ss

Sago Pondw

eed

Curly-L

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Eurasia

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Slende

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Larg

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June-08 July-08

Figure 10 and Figure 11 show site frequencies for all plants collected in Basins #2 and #3 during 2007 and 2008. Basins #2 and #3 were treated with 2, 4-D in 2007 and Renovate in 2008. These graphs offer a before and after picture for the 2007 and 2008 herbicide treatments. Twelve total species were collected in 2007 in Basins 2 and 3 while 10 total species were found in Basins #2 and #3 in 2008. Flat-stemmed pondweed and Illinois pondweed were collected in low abundance in 2007 but were not collected in 2008. Coontail site frequency in Basins #2 and #3 increased slightly in 2007 after the 2, 4-D treatment and decreased slightly in 2008 after the Renovate treatment. Eurasian watermilfoil site frequency declined in both 2007 and 2008. Small pondweed, slender naiad, and eel grass all showed increases in sight frequency in both 2007 and 2008.

Figure 10: 2007 Basins 2 and 3 Site Frequencies

Big Lake Basins 2 and 3 2007 Site Frequencies (Partially Treated with 2, 4-D)

0 0 07.1 3.6 0

32.1

0 0 0 3.6

57.1

28.6 28.617.9 21.4

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Figure 11: 2008 Basins 2 and 3 Site Frequencies

Big Lake Basins 2 and 3 2008 Site Frequencies (Partially Treated with Renovate)

50

35.728.6

21.4

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Figure 12 describes pre- and post treatment site frequencies in Basin #1 for 2009. Areas of Basin #1 were treated with 2, 4-D in 2009. Eurasian watermilfoil abundance decreased from 15.6% before treatment to 0% after treatment. Coontail frequency increased from 53.1% before treatment to 65.6% after treatment. Eel grass also showed a large increase in frequency from spring to summer. Figure 12: 2009 Basin 1 Site Frequencies

53.1

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15.69.4

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Big Lake Basin 1 2009 Site Frequencies (Partially Treated with 2, 4-D)

Spring 2009 Fall 2009

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Figure 13 describes pre and post treatment site frequencies in basins #2 and #3 in 2009. These areas were treated with Renovate in 2009. Eurasian milfoil decreased from 25% in spring to 0 in summer. Such a complete EWM reduction was not expected. Algal growth and rising water temperatures in summer could also help account for such a dramatic reduction in EWM growth. Coontail frequency decreased from 71.4% before treatment to 64.3% after treatment. Figure 13: 2009 Basins 2 and 3 Site Frequencies

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14.3 10.7 14.37.1 3.6

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Big Lake Basins 2 and 3 2009 Site Frequencies (Partially Treated with Renovate)

Spring 2009 Fall 2009

8.3 Macrophyte Inventory Discussion In 2009, 11 different species were collected in the spring Tier II survey and 8 species were collected in the late season Tier II survey. Total species richness for the year was 11 species, which is very similar to past years. Twelve different species of submersed aquatic plants were collected in Big Lake during 2007 while 11 total plant species were collected between the two surveys in 2008. Eurasian watermilfoil and curly leaf pondweed are the two invasive species in Big Lake. They have been collected each year since 2006. Eurasian watermilfoil site frequency declined both lake-wide and by basin from spring to summer in 2007, 2008 and 2009. Eurasian watermilfoil was not collected in the 2009 late season Tier II survey. Eurasian watermilfoil site frequency in July of 2008 was 3.3% and EWM site frequency in August 2007 was 1.7%. It would appear that the current management strategy is maintaining Eurasian watermilfoil at a low level on an annual basis. Curly leaf pondweed abundance in spring of 2009 was 21.7% which was down from 25.0% in spring of 2008. In spring of 2007, curly leaf pondweed abundance was 15.0%. Curly leaf pondweed abundance should be tracked closely to ensure that it is not increasing in Big Lake as Eurasian watermilfoil is controlled each year. Coontail was the most frequently collected and most dominant plant in both 2009 Tier II surveys. Coontail has consistently been the most abundant plant in Big Lake. It was the most

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dominant plant in three of the four surveys in 2007 and 2008. In 2009, coontail site frequency increased slightly through the year. Its site frequency was 61.7% in spring and 65.0% in the late season survey. Eel grass, slender naiad, and large leaf pondweed all showed increases in site frequency from spring to summer in 2009. Species diversity in June 2009 was 0.83. Species diversity decreased slightly in the 2009 late season survey to 0.75. These values are very consistent with past diversity indices for Big Lake. Species diversity in May 2007 was 0.72 and increased to 0.81 in August 2007. Species diversity in June 2008 was 0.84 and declined slightly to 0.81 in late July of 2008. Diversity values from the past 4 years are relatively stable. It appears that species diversity in Big Lake is either remaining stable or increasing slightly and is above the average diversity value (0.66 – Pearson, 2004) that Pearson found in a study of 21 northern Indiana Lakes. Renovate vs. 2, 4-D Treatments on Big Lake

2009 was the third year of Renovate and 2, 4-D treatments for the control of Eurasian watermilfoil on Big Lake. In 2007, EWM infested areas in Basin #1 were treated with Renovate and infested areas in Basins # 2 and #3 were treated with 2, 4-D. This was an accidental switch from the original plan to treat with 2, 4-D in Basin #1 and Renovate in Basins # 2 and #3. In 2008 and 2009, 2, 4-D was used in Basin #1 and Renovate was used in Basins #2 and #3. Figure 14 describes 2009 treatment areas. Figure 14: Big Lake 2009 Treatment Areas

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Eurasian Watermilfoil: Renovate vs 2, 4-D Renovate Site frequency of Eurasian watermilfoil (EWM) in Basin #1 declined from 15.6% before treatment in May 2007 to 0 in August after the Renovate treatment. Site frequency of EWM in Basins #2 and #3 declined from 21.4% in June 2008 before treatment to 3.6% in August after the Renovate treatment. In 2009, site frequency of EWM in Basins #2 and #3 was 25.0% before treatment. EWM was not found after the Renovate treatment. 2, 4-D In 2007, EWM site frequency in Basins #2 and #3 declined from 32.1% before treatment to 3.6% after the 2, 4-D treatment. In 2008, EWM declined from 9.4% to 3.1% in Basin #1 after the 2, 4-D treatment. In 2009, EWM in Basin #1 had a site frequency of 15.6% before treatment. Basin #1 was then treated with 2, 4-D and EWM was not found after treatment. It would appear that both herbicides are effectively controlling Eurasian watermilfoil in Big Lake. However, one interesting note is that surveys by both Aquatic Weed Control and the IDNR in 2006 appear to indicate that Eurasian watermilfoil in Big Lake shows some natural die off as the summer progresses. Coontail: Renovate vs 2, 4-D Renovate In 2007 Basin #1 was treated with Renovate and coontail frequency dropped from 43.8% before treatment to 37.5% after the treatment. In 2008, coontail in Basins #2 and #3 declined from 50% before treatment to 46.4% after the Renovate treatment In 2009 coontail site frequency in basins #2 and #3 was 71.4% before the Renovate treatment. After the Renovate treatment, coontail frequency was 64.3%. 2,4-D In 2007, coontail site frequency in Basins #2 and #3 increased from 53.6% in May to 57.1% in August of 2007 after the 2, 4-D treatment. IDNR Vegetation surveys showed a slight decline in coontail in Basins #2 and #3 from 68% in May 2007 to 61% in August. In 2008, coontail frequency in Basin #1 declined from 50% before the 2, 4-D treatment to 46.9% after the 2, 4-D treatment.

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In 2009, coontail frequency in Basin 1 was 53.1% before the 2, 4-D treatment. After the 2, 4-D treatment, coontail frequency increased to 65.6%. Figure 15 and Figure 16 illustrate the effects of Renovate and 2, 4-D on both Eurasian watermilfoil and coontail in the different basins of Big Lake over the past three years. Pre-treatment data points display cross-hatching while post-treatment data points are shown as solid bars. Figure 15:Renovate Herbicide Effects on Coontail and EWM

43.850.0

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Basins 2-3 Coontail (2008)

Basins 2-3 Coontail (2009)

Basin #1 EWM (2007)

Basins 2-3 EWM (2008)

Basins 2-3 EWM (2009)

Big Lake Renovate Treatments Pre and Post Treatment Site Frequencies

Pre-Treatment

Post-Treatment

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Figure 16: 2, 4-D Effects on Coontail and EWM

53.650

53.1

32.1

9.4

15.6

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46.9

65.6

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20.0

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Basins 2-3 Coontail (2007)

Basin #1 Coontail (2008)

Basin #1 Coontail (2009)

Basins 2-3 EWM (2007)

Basin #1 EWM (2008)

Basin #1 EWM (2009)

Big Lake 2, 4-D TreatmentsPre and Post Treatment Site Frequencies

Pre-Treatment

Post-Treatment

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Table 9 and Table 10 show site frequencies of coontail and Eurasian watermilfoil in the different basins during Tier II surveys conducted by both Aquatic Weed Control and the IDNR (Pearson, 2007). The distribution of EWM in Big Lake is patchy and it is difficult to determine exact acreages of infestation. The best measure of EWM infestation may be site frequency. Table 9: AWC Eurasian Watermilfoil and Coontail Data

AWC May 2006

August 2006

May 2007

August 2007

June 2008

July 2008

June 2009

July 2009

Coontail Basin 1 No

survey 65.6 43.8 37.5 50.0 46.9 53.1 65.6

Basins 2 and 3 53.6 53.6 57.1 50.0 46.4 71.4 64.3 Eurasian Watermilfoil

No Survey

Basin 1 9.4 15.6 0 9.4 3.1 15.6 0 Basins 2 and 3 14.3 32.1 3.6 21.4 3.6 25.0 0

Table 10: IDNR Eurasian Watermilfoil and Coontail Data

IDNR May 2006 August 2006

May 2007

August 2007

July 2008

July 2009

Coontail Basin 1 78 63 53 44 66 Basins 2 and 3 75 75 68 61 60.7 64 Eurasian Watermilfoil

Basin 1 66 9 34 0 0 Basins 2 and 3 64 14 36 0 0 0

Conclusions Renovate and 2, 4-D have both demonstrated effective seasonal control of EWM on Big Lake. They have had similar effects, if any, on native plant species. Aquatic Weed Control recommends that this same treatment strategy be used in 2010 to give consistency through Big Lake’s fourth and final year of treatment funding priority. This approach would provide four years of data on this comparison and provide project consistency through Big Lake’s fourth and final year of treatment funding priority. Given LARE’s interest in comparing these two herbicides, keeping this treatment strategy consistent for 2010 seems practical.

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Threatened and Endangered Species

The Indiana Natural Heritage Data Center has compiled a list of Indiana plant species that are federally or state listed as endangered, threatened or rare. The following is an excerpt taken directly from the Indiana Natural Heritage Database website. Link: Indiana Natural Heritage Data Center. “The Indiana Natural Heritage Data Center, set up in 1978, represents a comprehensive attempt to determine the state's most significant natural areas through an intensive statewide inventory. The Indiana Natural Heritage Data Center is part of the Natural Heritage Network, a worldwide system of Heritage Programs. This program is designed to provide information about Indiana's diversity of natural ecosystems, species, landscape features, and outdoor amenities, and to assure adequate methods for evaluating this information and setting sound land protection priorities. The inventory is a continuous process, becoming an increasingly valuable tool for decision makers and scientists as it progresses.” No state or federally listed plant species were found in Big Lake in 2009. No vouchers were taken that were suspected to be endangered species.

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9.0 Aquatic Plant Management Alternatives Management practices for the control of Eurasian watermilfoil have not changed significantly since the 2006 lake management plan. No Treatment: If no treatments are conducted on Big Lake in the future, it is very difficult tosay what might happen. There is a large section of the lake where EWM will not grow because of water depth. Native plants may compete well with the milfoil in Big Lake, or Eurasian watermilfoil could take over the existing littoral zone to create a monoculture as has been seen in other lakes. Renovate and 2, 4-D Treatments: The IDNR is currently using Big Lake to test for differences in treatment efficacy and damage to native plants species in Renovate and 2,4-D treatments. Both of these herbicides are commonly used for spot treatments of Eurasian watermilfoil. They are both systemic herbicides, meaning they are translocated from the foliage of the plant into the root system. Renovate is more expensive than 2, 4-D, although the chemistries of the two products are very similar. The justification for the extra expense is that Renovate is said to have the potential for multiple years of control on Eurasian milfoil. It is also said that Renovate may have less impact on native species like coontail. Although treatments using both of these herbicides are common on both public and private waters in Indiana, the IDNR would like to study the effects of these two herbicides in the same body of water. In 2009, areas of Eurasian watermilfoil in Basin #1 of Big Lake were treated with 2, 4-D, while areas of Eurasian watermilfoil in Basins #2 and #3 were treated with Renovate. 10.0 Public Involvement Figure 17 summarizes the public questionnaire data received from input at the public meeting. The public meeting for Big Lake was held on August 29, 2009. Twenty-three people filled out public questionnaires. Residents were concerned over the availability of continued LARE funding and LARE prioritization for future treatments as well as overall project costs. Other concerns include nutrient and sediment inputs, and a possible decrease in fishing success over the past few years. Residents were also concerned about Large-leaf pondweed abundance, especially in basin 1. The Big Lake Association is active, and lake association meetings help to keep the public informed about management practices on Big Lake. Other avenues that may be used to inform the public would be periodic newsletters, an email list, an association website, or posting signs at public access sites.

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Figure 17: Big Lake 2009 Public Questionnaire

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11.0 Public Education

The Big Lake Association has been very aggressive in preventing the spread of invasive aquatic vegetation. They have privately helped to fund herbicide treatments and have submitted a proposal to the LARE program for additional herbicide treatment of Eurasian watermilfoil. These herbicide treatments reduced Eurasian watermilfoil frequency in 2007 through 2009.

More information on stopping the spread of invasive aquatic organisms can be found at http://www.protectyourwaters.net/. These items include thoroughly cleaning equipment after use in a lake and removing all water from bilges, livewells, etc.

Hydrilla

Hydrilla (Hydrilla verticillata) is an invasive aquatic plant species common throughout the southern United States. It federally listed as a noxious weed and causes severe ecological and

recreational problems wherever it grows. It is considered to be much more destructive than other invasives like Eurasian watermilfoil and curly leaf pondweed because of its reproductive adaptations. It grows by fragmentation, as does Eurasian watermilfoil, but it also produces turions which can remain dormant in the sediment for 4 years or more (Van and Steward, 1990). It produces tubers at its root tips which can also reproduce after multiple years of dormancy. It can grow 1 inch each day and it quickly out-competes native plants. It forms dense beds that eliminate native plants, stunt fish populations, impede recreation and cause a drastic decrease in biodiversity (Colle and Shireman, 1980). Millions of dollars are spent each year for hydrilla maintenance each year in Florida alone. Eradication is unlikely once a population has been well established, although eradication has been achieved in

newly infested waters using a herbicide called Sonar. Sonar is applied at a rate of 6 parts per billion and this concentration is maintained in the water for 180 days. Early detection can be

crucial to an effective eradication program, and all lake residents and users are encouraged to be on the look-out for this invader. In summer of 2006, this plant was found in Lake Manitou, in Rochester, Indiana. This is the first instance of hydrilla in the upper Midwest. Prior to its appearance in Lake Manitou, The closest infestations of hydrilla were in Tennessee and Pennsylvania. Hydrilla can easily be confused with native elodea. The major difference is that elodea has sets of leaves on the stem in whorls of three, while hydrilla usually has whorls of 5 leaves, although 4 to 9 leaves per whorl are possible with hydrilla. Hydrilla will also have small serrations on the leaf edges. More information on hydrilla can be found at the University of Florida’s Center for Aquatic Invasive Plants (http://plants.ifas.ufl.edu/). More general

information on aquatic invaders can be found at www.protectyourwaters.net.

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12.0 Integrated Treatment Action Strategy

Based on the data analysis from Tier II surveys over the past 3 years, the current herbicide treatment program appears to be providing adequate seasonal control of Eurasian watermilfoil in Big Lake. It is recommended that the same treatment strategy be implemented in 2010. Using the same treatment strategy in 2010 will provide consistency through Big Lake’s four years of treatment funding priority while giving four years of data for the important comparison of Renovate and 2, 4-D herbicides. Big Lake has presented a unique opportunity to study two different herbicides on the same body of water. Aquatic Weed Control recommends that the same treatment strategy be used for one more year (2010). The current strategy call for treating up to 18 acres in basin 1 of Big Lake with DMA-4 (2, 4-D) herbicide. It also calls for treating up to 22 acres in basins 2 and 3 of Big Lake with Renovate (triclopyr) herbicide for control of Eurasian watermilfoil. Exact treatment areas would depend upon Eurasian watermilfoil distribution in spring of 2010. Both a pre-treatment spring Tier II survey and a post-treatment, late season Tier II survey are recommended for Big Lake in 2010 to accurately map Eurasian watermilfoil distribution and further study potential differences between Renovate and DMA-4 treatments.

The 2, 4-D and Renovate treatments conducted in 2007 through 2009 were successful at reducing Eurasian watermilfoil abundance, but it is very important for all parties to understand that although 2, 4-D and Renovate treatments provide very effective EWM control they only provide season long control. In 2010, Eurasian watermilfoil is expected to return to the 2009 treatment areas. Renovate and 2, 4-D cannot be expected to eradicate Eurasian watermilfoil in Big Lake. Maintenance of the Eurasian watermilfoil must be conducted on a yearly basis with this treatment program. Eurasian watermilfoil abundance is not expected to decline each year.

It is important to note that Eurasian watermilfoil will be the only plant species specifically targeted in this project, as LARE funds will be awarded only for the control of invasive plant species. The goal is not to eliminate vegetation in Big Lake, but to improve the health of the plant community by reducing EWM abundance on an annual basis. Native vegetation will still be abundant in shallow areas after treatment, and control of these natives must be privately funded. The goal will be to provide yearly maintenance of the EWM population and allow for the recovery of native plant species that will provide better fish habitat, foster good water quality and pose less interference to recreational use of the lake.

Private treatments for coontail could be permitted in 2010, but would be considered by the IDNR on a case by case basis. Jed Pearson, District 3 Fisheries Biologist, may inspect areas of concern in 2009 to determine if they may be eligible for an aquatic vegetation control permit.

Herbicide Treatment Specifications

If 2, 4-D is used for herbicide treatments, then a concentration of 1.76 parts per million should be used to ensure adequate control. If Renovate is used, then the concentration should be at or near 1.5 parts per million.

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Lake and River Enhancement Deadlines

December 15 – Rough drafts of LARE AMVPs and AVMP updates due to LARE staff January 15 – Grant application due to LARE Staff February 15 – Revisions of AVMPs and updates due back to contractors March 1 – Final drafts of AVMPs and AVMP updates due to LARE Staff March 15 – LARE funding decisions announced 13.0 Project Budget Original cost estimates for 2008 through 2011 are included in the 2007 AVMP. An effort will be made to maintain costs as close to these original estimates as possible. These figures are estimates only and are subject to change pending future chemical pricing. 2010 Proposed Budget Treat up to 18 acres in Basin #1 with 2, 4-D for Eurasian watermilfoil: up to $6,660 Treat up to 22 acres in Basins # 2 and #3 with Renovate for Eurasian watermilfoil: up to $10,780

2010 Surveys and Planning Two Tier II vegetation surveys are recommended for 2010. A spring pre-treatment survey and a late season post-treatment survey will take place. 2010 Tier II surveys and management plan update up to $6,300

2010 Total Cost Estimates Basin #1 treatment $6,660 Basin #2 and 3 Treatment $10,780 Tier II Surveys and plan update $6,300 Total maximum project costs $23,740 Lake and River Enhancement Share $21,366 Big Lake Association’s Share $2,374

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14.0 Monitoring and Plan Update Procedures Two Tier II vegetation surveys are recommended for Big Lake in 2010 to further study the difference between EWM spot treatments using Renovate vs. 2, 4-D. One survey will take place in spring prior to herbicide treatments. Data from this survey will be used to develop a treatment map for Eurasian watermilfoil in Big Lake. Herbicide treatments using 2, 4-D and Renovate will follow, not to exceed 18 acres in Basin#1 and 22 acres in Basins #2 and #3. The second survey will take place after the herbicide treatments. The post treatment survey should be conducted in late summer to allow the slow acting herbicides to achieve full control before the survey is conducted. Surveys of the emergent plant community may also be a possibility for the future. Costs for an emergent survey are not included in the current budget, but these surveys could be added to the update procedures in the future.

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15.0 References Colle DE, Shireman JV. 1980. Coefficients of condition for largemouth bass, bluegill and redear sunfish in hydrilla-infested lakes. Transactions of the American Fisheries Society 109:521-531. Dow Agrosciences Invasive Species Management. 1998-2007. Dow Agrosciences LLC. http://www.dowagro.com/ivm/invasive/prod/dma.htm IDNR. 2004. Procedure Manual for Surveying Aquatic Vegetation: Tier II Reconnaissance Surveys. IN Department of Natural Resources, Division of Soil Conservation. IDNR 2004. Procedure manual for surveying Aquatic Vegetation: Tier I and Tier II, Indiana Department of Natural Resources, Indianapolis, Indiana. Pearson. 2007. Big Lake 2006 and 2007 Survey Data. IN Department of Natural Resources. Division of Fish & Wildlife. Indianapolis, Indiana. Pearson, Jed. 2004. A Proposed Sampling Method to Assess Occurrence, Abundance and Distribution of Submersed Aquatic Plants in Indiana Lakes. IN Department of Natural Resources. Division of Fish & Wildlife. Indianapolis, Indiana 37 pp. Pullman, Douglas G. 1998. The Lake Association Leaders Aquatic Vegetation Management Guidance Manual. Renovate 3 Specimen Label. 2003. SePRO Corporation. www.sepro.com Smith, Robert Leo and Smith, Thomas M. 2001. Ecology and Field Biology. Addison Wesley Longman, Inc. San Francisco, California. 771 pp. Van TK, Steward KK. 1990. Longevity of monoecious hydrilla propagules. J. Aquat. Plant Manage. 28:74-76 16.0 Appendices 16.1 Calculations Fluridone Calculations: The following paragraph is taken directly from the Sonar A.S. label. It outlines the specific procedures for calculating the amount of Fluridone needed to treat a body of water. Application Rate Calculation - Ponds, Lakes and Reservoirs The amount of Sonar A.S. to be applied to provide the desired ppb concentration of active ingredient in treated water may be calculated as follows: Quarts of Sonar A.S. required per treated surface acre =

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Average water depth of treatment site (feet) x Desired ppb concentration of active ingredient x 0.0027 For example, the quarts per acre of Sonar A.S. required to provide a concentration of 25 ppb of active ingredient in water with an average depth of 5 feet is calculated as follows: 5 x 25 x 0.0027 = 0.33 quarts per treated surface acre When measuring quantities of Sonar A.S., quarts may be converted to fluid ounces by multiplying quarts to be measured x 32. For example, 0.33 quarts x 32 = 10.5 fluid ounces. Note: Calculated rates should not exceed the maximum allowable rate in quarts per treated surface acre for the water depth listed in the application rate table for the site to be treated.

The following chart outlines rate calculations for DMA – 4 IVM Herbicide. It was taken directly from the DMA – 4 IVM specimen label on Dow AgroSciences website. http://www.dowagro.com/ivm/invasive/prod/dma.htm

The following table outlines rate calculations for Renovate 3 herbicide based on desired PPM and average depth of treatment area. It is taken directly from the Renovate 3 specimen label on SePRO Corporation’s website: www.sepro.com

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16.2 Common Aquatic Plants of Indiana (See 2006 Lake Management Plan) 16.3 Pesticide Use Restrictions Summary: The following table was produced by Purdue University and included in the Professional Aquatic Applicators Training Manual. It gives a summary of water use restrictions on all major chemicals available for use in the aquatics market. Table 11: Pesticide Use Restrictions

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16.4 Resources for Aquatic Management In addition to the LARE Program, there are many other sources of potential funding to help improve the quality of Indiana Lakes. Many government agencies assist in projects designed to improve environmental quality. The USDA has many programs to assist environmental improvement. More information on the following programs can be found at www.usda.gov.

Watershed Protection and Flood Prevention Program (USDA Conservation Reserve Program (USDA) Wetlands Reserve Program (USDA) Grassland Reserve Program (USDA) Wildlife Habitat Incentive Program (USDA) Small Watershed Rehabilitation Program (USDA)

The following programs are offered by the U.S. Fish and Wildlife Service. More information about the Fish and Wildlife service can be found at www.fws.gov

Partners for Fish and Wildlife Program (U.S. Fish and Wildlife Service) Bring Back the Natives Program ( U.S. Fish and Wildlife Service) Native Plant Conservation Program (U.S. Fish and Wildlife Service)

The Environmental Protection Agency, the Indiana Department of Environmental Management, and the U.S. Forest Service also have numerous programs for funding. A few of these are listed below. More information can be found at www.in.gov/idem and www.fs.fed.us/ U.S. Environmental Protection Agency Environmental Education Program (EPA) NPDES Related State Program Grants (IDEM) Community Forestry Grant Program (U.S. Forest Service)

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16.5 State Regulations for Aquatic Plant Management The following information is found on the IDNR website and outlines general regulations for the management of aquatic plants in public waters.

AQUATIC PLANT CONTROL PERMIT REGULATIONS Indiana Department of Natural Resources

Note: In addition to a permit from IDNR, public water supplies cannot be treated without prior written approval from the IDEM Drinking Water Section. Amended state statute adds biological and mechanical control (use of weed harvesters) to the permit requirements, reduces the area allowed for treatment without a permit to 625 sq ft, and updates the reference to IDEM. These changes become effective on July 1, 2002. Chapter 9. Regulation of Fishing IC 14-22-9-10 Sec. 10. (a) This section does not apply to the following: (1) A privately owned lake, farm pond, or public or private drainage ditch. (2) A landowner or tenant adjacent to public waters or boundary waters of the state, who chemically, mechanically, or physically controls aquatic vegetation in the immediate vicinity of a boat landing or bathing beach on or adjacent to the real property of the landowner or tenant if the following conditions exist: (A) The area where vegetation is to be controlled does not exceed: (i) twenty-five (25) feet along the legally established, average, or normal shoreline; (ii) a water depth of six (6) feet; and (iii) a total surface area of six hundred twenty-five (625) square feet. (B) Control of vegetation does not occur in a public waterway of the state. (b) A person may not chemically, mechanically, physically, or biologically control aquatic vegetation in the public waters or boundary waters of the state without a permit issued by the department. All procedures to control aquatic vegetation under this section shall be conducted in accordance with rules adopted by the department under IC 4-22-2. (c) Upon receipt of an application for a permit to control aquatic vegetation and the payment of a fee of five dollars ($5), the department may issue a permit to the applicant. However, if the aquatic vegetation proposed to be controlled is present in a public water supply, the department may not, without prior written approval from the department of environmental management, approve a permit for control of the aquatic vegetation. (d) This section does not do any of the following: (1) Act as a bar to a suit or cause of action by a person or governmental agency. (2) Relieve the permittee from liability, rules, restrictions, or permits that may be required of the permittee by any other governmental agency. (3) Affect water pollution control laws (as defined in IC 13-11-2-261) and the rules adopted under water pollution control laws (as defined in IC 13-11-2-261). As added by P.L.1-1995, SEC.15. Amended by P.L.1-1996, SEC.64. 312 IAC 9-10-3 Aquatic vegetation control permits Authority: IC 14-22-2-6; IC 14-22-9-10 Affected: IC 14-22-9-10 Sec. 3. (a) Except as provided under IC 14-22-9-10(a), a person shall obtain a permit under this section before applying a substance to waters of this state to seek aquatic vegetation control. (b) An application for an aquatic vegetation control permit shall be made on a departmental form and must include the following information: (1) The common name of the plants to be controlled. (2) The acreage to be treated. (3) The maximum depth of the water where plants are to be treated. (4) The name and amount of the chemical to be used. (c) A permit issued under this section is limited to the terms of the application and to conditions imposed on the permit by the department. (d) Five (5) days before the application of a substance permitted under this section, the permit holder must post clearly, visible signs at the treatment area indicating the substance that will be applied and

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what precautions should be taken. (e) A permit issued under this section is void if the waters to be treated are supplied to the public by a private company or governmental agency. (Natural Resources Commission; 312

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16.6 Species Distribution Maps

Figure 18: Big Lake Rake Sample Locations

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Figure 19: Big Lake Spring 2009 Elodea Locations

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Figure 20: Big Lake Spring 2009 Eel Grass Locations

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Figure 21: Big Lake Spring 2009 Curly Leaf Pondweed Locations

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Figure 22: Big Lake Spring 2009 Coontail Locations

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Figure 23: Big Lake Spring 2009 Chara Locations

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Figure 24: Big Lake Spring 2009 Small Pondweed Locations

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Figure 25: Big Lake Spring 2009 Slender Naiad Locations

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Figure 26: Big Lake Spring 2009 Sago Pondweed Locations

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Figure 27: Big Lake Spring 2009 Large Leaf Pondweed Locations

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Figure 28: Big Lake Spring 2009 Flat-stemmed Pondweed Locations

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Figure 29: Big Lake Spring 2009 Eurasian Watermilfoil Locations

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July 2009

Figure 30: Big Lake Summer 2009 Eel Grass Locations

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Figure 31: Big Lake Summer 2009 Coontail Locations

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Figure 32: Big Lake Summer 2009 Chara Locations

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Figure 33: Big Lake Summer 2009 Small Pondweed Locations

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Figure 34: Big Lake Summer 2009 Slender Naiad Locations

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Figure 35: Big Lake Summer 2009 Sago Pondweed Locations

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Figure 36: Big Lake Summer 2009 Large Leaf Pondweed Locations

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Figure 37: Big Lake Summer 2009 Elodea Locations

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16.7 Data Sheets Figure 38: Spring 2009 Data Cover Sheet

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Figure 39: Spring 2009 Data Sheet 1

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Figure 40: Spring 2009 Data Sheet 2

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Figure 41: Summer 2009 Data Cover Sheet

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Figure 42: Summer 2009 Data Sheet 1

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Figure 43: Summer 2009 Data Sheet 2

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Sample Location GPS Coordinates Table 12: GPS Coordinates for Rake Sample Locations

1 R 41.27021 -85.49364

2 R 41.27185 -85.49388

3 R 41.27479 -85.49352

4 R 41.27577 -85.49520

5 R 41.27754 -85.49727

6 R 41.27967 -85.49776

7 R 41.28094 -85.50007

8 R 41.28091 -85.50303

9 R 41.27966 -85.50455

10 R 41.27744 -85.50346

11 R 41.27593 -85.50339

12 R 41.27450 -85.50204

13 R 41.27423 -85.50382

14 R 41.27505 -85.50671

15 R 41.27613 -85.50809

16 R 41.27710 -85.50597

17 R 41.27767 -85.51039

18 R 41.27569 -85.51146

19 R 41.27549 -85.50946

20 R 41.27390 -85.50822

21 R 41.27275 -85.50598

22 R 41.27279 -85.50390

23 R 41.27223 -85.50314

24 R 41.27263 -85.50142

25 R 41.27157 -85.49912

26 R 41.27336 -85.49981

27 R 41.27499 -85.49954

28 R 41.27332 -85.49802

29 R 41.27043 -85.49513

30 R 41.26997 -85.49415

31 R 41.27240 -85.49403

32 R 41.27538 -85.49485

33 R 41.27632 -85.49657

34 R 41.27832 -85.49727

35 R 41.28175 -85.50207

36 R 41.28003 -85.50393

37 R 41.27863 -85.50368

38 R 41.27686 -85.50339

39 R 41.27517 -85.50240

40 R 41.27372 -85.50243

41 R 41.27578 -85.50594

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42 R 41.27559 -85.50877

43 R 41.27732 -85.50745

44 R 41.27672 -85.51191

45 R 41.27565 -85.50989

46 R 41.27314 -85.50756

47 R 41.27267 -85.50532

48 R 41.27250 -85.49997

49 R 41.27444 -85.49888

50 R 41.27182 -85.49657

51 R 41.27398 -85.49435

52 R 41.27999 -85.49844

53 R 41.28035 -85.50317

54 R 41.27521 -85.50530

55 R 41.27726 -85.50799

56 R 41.27560 -85.51080

57 R 41.27497 -85.50872

58 R 41.27216 -85.50119

59 R 41.27496 -85.50055

60 R 41.27402 -85.49824

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16.8 LARE Resume

Aquatic Weed Control ____________________________________________________________ P.O. Box 325 Syracuse, IN 46567 Phone: (574) 533-2597 Fax: (574) 534-8230 Email: [email protected]

Services:

Herbicide Treatment Aquatic Plant Surveys Aquatic Vegetation Management Plans

Jim Donahoe: Owner/Operator

Purdue University, West Lafayette, IN, Bachelor of Science: Agricultural Marketing

19 years as a state licensed chemical applicator and owner of Aquatic Weed Control David Keister: Staff Biologist and licensed chemical applicator

Bethel College, Mishawaka, IN, Bachelor of Science: Environmental Biology

The Ohio State University, Columbus, OH, Ichthyology and Limnology classes at F.T. Stone Laboratory, Lake Erie.

Equipment: Aquatic Weed Control possesses all essential components needed to complete aquatic plant surveys, aquatic management plans and herbicide treatments.

Survey and application boats WAAS enabled GPS Temperature and dissolved oxygen meters Lowrance Sonar Range Finders GPS Mapping Software Data Analysis Software Computers Laser Printers/scanners/copiers Aquatic vegetation sampling rake Plant Identification keys

Projects: Aquatic Weed Control has been contracted to conduct vegetation surveys and write aquatic vegetation management plans for 9 separate Indiana Lakes. Each of these plans have been approved by the Lake and River Enhancement (LARE) biologists. Aquatic

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Weed Control has conducted all chemical applications that have been funded by LARE on these lakes. The following list includes contact information for every LARE funded project conducted by Aquatic Weed Control.

Cree Lake The Cree Lake Association 10686 North D Drive Kendallville, IN 46755 Services: Aquatic Vegetation Management Plan (no chemical treatment necessary)

Dewart Lake The Dewart Lake Protective Association Inc. P.O. Box 152 Syracuse, IN 46567 Services: Aquatic Vegetation Management Plan Whole Lake Eurasian watermilfoil treatment for 2006 funded by LARE. Contact: Mr. Mike Gill 58 EMS Lane D12 Syracuse, IN 46567 (574) 658- 4766

Lake Manitou

The Lake Manitou Association 1618 Bessmore Park Road Rochester, IN 46975 Services: Aquatic Vegetation Management Plan and update Conducted spot treatments of Eurasian watermilfoil. Contact: Orv Huffman 1618 Bessmore Park Road Rochester, IN 46975

Lake of the Woods

The Lake of the Woods Property Owners Association 3119 Sea Lane Bremen, IN 46506 Services: Aquatic Vegetation Management Plan and update

Conducted a whole-lake treatment for Eurasian watermilfoil. Contact: Mrs. Sharon Galminas 3119 Sea Lane Bremen, In 46506 (574) 546-4100

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Lake Wawasee The Wawasee Area Conservancy Foundation P.O. Box 548 Syracuse, IN 46567 Services: Aquatic Vegetation Management Plan Contact: Heather Harwood P.O. Box 548 Syracuse, IN 46567 (574) 457-4549

Silver Lake The Silver Lake Association 3332 West Neher Road Silver Lake, IN 46982 Services: Aquatic Vegetation Management Plan Conducted an early season curly leaf treatment of the entire littoral zone.

Skinner Lake The Skinner Lake Association 2916 East Skinner Lake Road Albion, IN 46701 Services: Aquatic Vegetation Management Plan (no chemical treatment necessary)

Syracuse Lake The Syracuse Lake Association P. O. Box 12 Syracuse, IN 46567 Services: Aquatic Vegetation Management Plan Conducted spot treatments for Eurasian watermilfoil

Waubee Lake The Waubee Lake Association P.O. Box 275 Milford, IN 46542 Services: Aquatic Vegetation Management Plan Conducted spot treatments for Eurasian watermilfoil

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16.9 IDNR Aquatic Vegetation Control Permit This page intentionally left blank.

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