The balancing act. Redox poise and signalling

http://plantcell.lu.se/balancing

AcknowledgementsLund University Plant Cell Biology-Plant Biochemistry http://plantcell.lu.se

Thomas Pfannschmidt (Now University of Jena, Germany)

Dalibor Stys (Now S. Bohemia University, Czech Republic)

Anders Nilsson (Now Bruker Spectroscopy, Lund)

Sylvan Dubé (Now Laval University, Canada)

Paul Davison (Now Sheffield University, U.K.)

Jens Forsberg

Jörgen Ström

Magnus Rosenquist (Sommarin lab)

Laure Fraysse (Kjellbom lab)

H. Louise Race (Now Aylesbury, U.K.)

Collaborators

Torbjörn Drakenberg, Sture Forsén (Lund Physical Chemistry 2)

Gerhard Link (University of Bochum, Germany)

James A. Sullivan, John C. Gray (University of Cambridge, U.K.)

Cartoons

Sarah Allen

The bucket

The searchlight Allen, J. F. (2001) EMBO Reports 2, 542-544

Light 2

Light 1

The balancing act1.

Transients, enhancement, and redox poise of cyclic electron flow.

Rates and activities of components.

Myers, J. (1971) Annu. Rev.Plant Physiol. 22, 289-312

Hill, R. (1965) Essays Biochem. 1, 121-151

Park, R. B. and Sane, P.V. (1971) Annu. Rev. Plant Physiol.22, 395-430

Albertsson, P.-Å. (2001) Trends Plant Sci. 6, 349-354

Pfannschmidt, T. et al. (1999) IUBMB Life 48, 271-276

The balancing act2.

Protein phosphorylation and state transitions. Post-translational

modification of the structure and function of proteins you already

have.

Allen, J. F. (1992) BBA 1098, 275-225 . Adapted from Allen, J.F. et al. (1981) Nature 291, 25-29

Updates on state transitions

• Wollman, F.-A. (2001) EMBO J. 20, 3623-3630

– “The kinase” and the site of redox control

• Kruse, O. (2001) Naturwissenschaften 88, 284-289– Structure and the PS II-LHC II supercomplex

• Haldrup, A. et al. (2001) Trends Plant Sci., 6, 301-305– Binding change, PS I and Psa-H

Time (s)

12

3

A B

C

D E F G H

Fi

Dubé, S., Davison, P.A. and Allen, J. F. (unpublished)

State 1State 2

P

P

P

P

P

State 1State 2

Allen, J. F. and Forsberg, J. (2001) Trends Plant Sci.6, 317-326

HP

P

H

P

Nilsson, A. et al. (1997) JBC 272, 18350-18357

The balancing act3.

Redox control of gene expression. Changing transcription and the stoichiometry of complexes.

Allen, J. F. (1992) BBA 1098, 275-225

Pfannschmidt, T. et al. (1999) IUBMB Life 48, 271-276

Pfannschmidt, T. et al. (1999) Nature 397, 625-628

The balancing act4.

Redox signalling and evolution.

Why chloroplasts (and mitochondria…) have genomes

Allen, J. F. (1993) J. Theor. Biol. 165, 609-631

Localization of Arabidopsis BAB09274 His Kinase-GFP fusion protein in tobacco leaf epidermal cells

Unpublished data of Sullivan, J. A., Gray, J.C. (Cambridge) Rosenquist, M., Forsberg, J., Allen, J. F. (Lund)

Protein ID Organism Type of two-component signallingelement

Predicted subcellular location Probability of location

ycf26 Porphyra purpurea Sensor histidine kinase Chloroplast 1*BAB09274 Arabidopsis thaliana Hybrid histidine kinase Chloroplast thylakoid membrane 0.922AAD03576 Arabidopsis thaliana Hybrid histidine kinase Chloroplast thylakoid membrane 0.632ycf27 Porphyra purpurea Response regulator Chloroplast 1*ycf29 Porphyra purpurea Response regulator Chloroplast 1*AAD15535 Arabidopsis thaliana Response regulator Mitochondrial matrix space 0.628AAD55287 Arabidopsis thaliana Response regulator Chloroplast stroma 0.864BAB20581 Zea mays Response regulator Chloroplast stroma 0.938BAA85112 Zea mays Response regulator Chloroplast thylakoid membrane 0.656BAA85113 Zea mays Response regulator Chloroplast stroma 0.600

Protein ID Organism Type of two-component signalling

element

Predicted subcellular location Probability of location

ycf26 Porphyra purpurea Sensor histidine kinase

Chloroplast 1*

BAB09274 Arabidopsis thaliana Hybrid histidine kinase Chloroplast thylakoid membrane

0.922

AAD03576 Arabidopsis thaliana Hybrid histidine kinase Chloroplast thylakoid membrane

0.632

ycf27 Porphyra purpurea Response regulator Chloroplast 1*

ycf29 Porphyra purpurea Response regulator Chloroplast 1*

AAD15535 Arabidopsis thaliana Response regulator Mitochondrial matrix space 0.628

AAD55287 Arabidopsis thaliana Response regulator Chloroplast stroma 0.864

BAB20581 Zea mays Response regulator Chloroplast stroma 0.938

BAA85112 Zea mays Response regulator Chloroplast thylakoid membrane

0.656

BAA85113 Zea mays Response regulator Chloroplast stroma 0.600

Two-component sensors and response regulators predicted to be in chloroplasts and mitochondria (by P-sort)

Forsberg, J. et al. (2001) Biochem. Soc. Trans. 29, 403-407

cpDNA-encoded in all sequenced genomes of photosynthetic plastids

cpDNA-encoded in at least one sequenced plastid genome

Cytochrome b6/f Photosystem IPhotosystem II

A

B

Race, H. L., Herrmann, R. G. and Martin, W. (1999) Trends Genet. 15, 364-370

The balancing act. Redox poise and signalling

• Transients, enhancement, and redox poise of cyclic electron flow.

• Protein phosphorylation and state transitions.

• Redox control of gene expression.

• Redox signalling and evolution.

Light 1

Light 2

The balancing act. Redox poise and signalling

http://plantcell.lu.se/balancing