The Alfin-like PHD Zinc Finger Transcription Factor

Family in Arabidopsis Thaliana

Melissa Borkenhagen8 May 2006

HC70ALDR. Bob Goldberg

Gene AT5G20510

5’ UTR

EXONINTRON

T-DNA

LBb1FW

Chromosome 5

T-DNA INSERT ACCORDING TO SALK

3’ UTR

Gene AT3G42790

3’ UTREXONINTRON

T-DNA

LBb1

FW

Chromosome 3

T-DNA INSERT ACCORDING TO SALK

5’ UTR

In which plant organs are the genes active?

Gene AT5G20510

Gene AT3G42790

Consistent with GeneChip Data, both genes appear to be active in these plant organs.

Gene AT5G20510 Plant Genotypes

Strange double bandsWhat are the genotypes?

Only one T-DNA Insert

Plant 1 is the only Homozygous Mutant Plant

Separation of primers

Gene AT3G42790 Plant Genotypes

Plants 1 and 4 are Homozygous Mutants

Plant 5: Heterozygous?

Are there any observable phenotypic differences between

wild type and mutant plants?

No significant differences observed between wild type and mutant plants.

Wild Type

Mutant

Is continued work on this family of transcription factors

important?Research has shown that members of the

alfin-like PHD zinc finger transcription factor family may be influential in plant

development. Some appear to be particularly influential during seed

development, with a possible role in the differential seedling development in darkness and light. Thus, continued work on these proteins is important!