27
National Centre of Excellence in Molecular Biology University of the Punjab Tender Ref:- CEMB NP 21- 2019 Dated:- 31 / 10 / 2019 Sealed tenders are invited from reputed General/Scientific Order suppliers/contractors so as to reach the undersigned by 11:00 am on 25 / 11 / 2019. The tenders will be opened on the same day at 12:00 noon in the presence of tenderers or their representatives. The Director CEMB reserves the right to reject any or all tenders. If their public holiday announced by the Government on the tender opening date, then the tender will be open on the next working day. This tender notice is available at www.ppra.org.pk and website of the Centre www.cemb.edu.pk. Senior Administrative Officer PH # 042-35293141-7 EXT:151

Tender Ref:- CEMB NP 21- 2019

  • Upload
    others

  • View
    3

  • Download
    0

Embed Size (px)

Citation preview

Page 1: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

Tender Ref:- CEMB NP – 21- 2019

Dated:- 31 / 10 / 2019

Sealed tenders are invited from reputed General/Scientific Order

suppliers/contractors so as to reach the undersigned by 11:00 am on 25 / 11 / 2019. The

tenders will be opened on the same day at 12:00 noon in the presence of tenderers or their

representatives. The Director CEMB reserves the right to reject any or all tenders. If their

public holiday announced by the Government on the tender opening date, then the tender

will be open on the next working day. This tender notice is available at www.ppra.org.pk

and website of the Centre www.cemb.edu.pk.

Senior Administrative Officer

PH # 042-35293141-7

EXT:151

Page 2: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

Terms & Conditions :-

1 Bidders are advised not to quote different options ( only one option is to be quoted). Revised bid with

different options shall not be entertained.

2 The Prices, including all taxes, should be quoted item-wise in Pakistan rupees.

3 Offers should be valid for 90 working days from the date of opening of the tender.

4 Delivery period will be (08) weeks from the date of placement of order. Eelier delivery will also be

acceptable.

5 Bidders must submit their tender duly signed, clearly indicating the offered specifications of each item,

specified in the tender documents.

6 The Centre reserves the right, at the time placing the order, to increase or decrease the quantity of items.

7 Bid must accompanied by (2%) of estimated cost of bid as earnest money in the shape of CDR in the name

of Director CEMB, Lahore without which bid will not be entertained. Bank guarantee is not acceptable.

8 The successful will arrange stamp paper @ 0.25% on total value of issuance of purchase order.

9 The supply must be in accordance with the offered specifications.

10

10% security of total value of purchase order/bill shall be deducted at the time of bill process. The deducted

amount as security will be returned after successful completion of defect liability / warranty period, after

repairing the defect in the equipment / replacement found during the warranty period.

11

In case of failure on the part of supplier to supply as per specifications quantity or within the stipulated time

without getting extension, penalty up to 2% of the supply order per month will be imposed on the supplier

by the head of department.

12 Payment will be made on availability of fund, if delayed due to any reason; no extra interest / markup will

be accepted or paid.

13 Deduction of income tax and other taxes of any kind will be deducted at the source according to

government prevailing rules.

14 After sale service and supply of equipment / parts must be guaranteed for a period of at least one year.

15

Any overwriting/crossing, etc. appearing in the offer may be properly signed by the person signing the

tender. All pages of the tender must be properly signed & stamped. Offer with any overwriting/use of

Blanco shall not be accepted in any circumstances.

16

In Case any supply/material is found not in conformity with the specifications provided in the tender, either

on account of inferior quality, defective workmanship, faulty design, faulty packing or is short supplied,, or

wrongly supplied, the supplier shall replace the same free of charges.

17 Procurement shall be governed by the PPRA Rules.

18 If there is any question/confusion about the description/specification of the material please visit

the Centre.

Senior Administrative Officer

PH # 042-35293141-7

EXT:151

Page 3: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

LP - 21 - 2019

TSN Item Description or Detail Qty Unit Price

( Inclusive of all taxes )

Computer

1 Battery for laptop HP Probook4410 01. No.

Pack Size

Brand

2 Toner Inject Color Printer 5560HP 05 Nos.

3 Toner HP Laserjet Pro MFP M25 05 Nos.

4 Re-Writable CDs 02 Pack

5 Toner HP 2035 01 No.

6 Toner HP 1320 (49A) 02 Nos.

7 Unifi UAP-AC-Pro 01 No.

8 Toner HP Laserjet Pro M401dn 01 No.

9 Toner HP2055 Laserjet 02 Nos.

10 Access Point Ubiquity unifi ap az pro 01 No.

11 Black Cartridge (45) Printer 1280 02 Nos.

Page 4: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

General

12 Stapler Heavy Duty 01 No.

Pack Size

Brand

13 Battery 5KVA UPS APC

CSB 5amp 12v 32 Nos.

14 Stapler Heavy Duty 01 Nos.

15 Compressor 1.5ton AC 01 No.

16 Compressor 1.5ton AC 01 No.

17 LED Tube Lights ( 40W ) 08 Nos.

18 Electric Switch Box + Plate

OPPO China fitting ( 10+10 ) 03 Nos.

19 Plastic samll box 06 Nos.

20 Plastic Medium Box 06 Nos.

21 Platic Large Box 06 Nos.

22 Pressure Guage for autocleave 02 Nos.

23 Temperature guage for autocleave 02 Nos.

24 Paper Cutter 02 Nos.

Page 5: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

25 Distilled Water Can 25ltrs 02 Nos.

26 Bleach Robin 500ml 150 Pack

27 Paper Envelop 3.8"x5.4" 1000 Nos.

28 Power Socket AKAI 30 Nos.

29 Telephone Set 05 Nos.

30 Mepore Dressing 9x10 box of 50 05 Box

31 Accu-Chek Active blood glucose meter 01 No.

32 Strips Accu-Chek Active blood glucose meter pack of

50 02 Pack

33 Syringe 10ml (Luer-Lok Tip) box of 100 02 Box

34 Syringe 10ml (Luer-Lok Tip) carton of 1000 03 Crtn

35 Syringe 10ml (Luer-Lok Tip) carton of 1000 04 Box

36 Cotton Swabs 05 Pack

37 Compressor 1.5ton 220V 01 No.

Page 6: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

Science

38 Ripa Buffer

Radioimmunoprecipitation assay buffer RO278-500ml 01 Bottle

Pack Size

Brand

39 Antibody β-catenin (E-5): 200 µg/ml

sc-7963. Santa Cruz Biotechnology 01 Pack

40

BD Medical 367884 Vacutainer® Plus Plastic Heparin

Blood Collection Tubes with Lithium Heparin for

Plasma Determination, 13 x 75mm, 4mL Draw Volume,

Paper Label and Green Hemogard™ Closure

02 Box

41 Culture, cell culture, tissue culture flask surface area

75 cm2, canted neck, cap (vented) z707848 03 Box

42 Lymphocyte separation medium - 500 mL L0560-500 02 Pack

43

PHA-M

(Phytohemagglutinin-M) Cell Culture Tested TC209-

25MG

01 Pack

44 Lipopolysaccharides from Escherichia coli O111:B4

L2630-10mg 01 Pack

45 Poly(A) Polymerase Ultra-pure enzyme for nucleic acid

modifications KEM0014 03 Pack

46 ATP Solution (100 mM) Cat No: R0441 0.25 mL 01 No.

47 GeneJET Plasmid Miniprep Kit Catalog number:

K0503 250 Preps 02 Kits

Page 7: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

48 Exo-SAP IT 02 Kits

49 Taq Polymerase 10000 Units

50 Kartogenin SML0370-25mg 02 Pack

51 Lipopolysaccharides L2630-100MG 01 Pack

52 Culture, cell culture, tissue culture flask surface area

75 cm2, canted neck, cap (vented) z707848 04 Case

53 NA1111-1KT GenElute(TM) Gel Extraction Kit

Sufficient For 70 Purifications 01 Kit

54 Crystal Violet, ≥90.0% Anhydrous Basis, ACS Reagent

Grade C6158-50G 01 Pack

55 SuperScript™ IV First-Strand Synthesis System Catalog

number: 18091050 50 Reactions 02 Pack

56 TRIzol™ LS Reagent Catalog number: 10296028 200

mL 01 Pack

57 Halt™ Protease Inhibitor Cocktail, EDTA-Free (100X)

Catalog number: 87785 1 mL 01 Pack

58

Dengue virus DENV-2 (Strain New Guinea C) Capsid

protein / DENV-C Antibody, Mouse Mab Cat: 40262-

MM05 Sino Biological

01 Pack

59 Anti-Dengue Virus prM glycoprotein antibody [DM1]

(ab41473) 1 ml 01 Pack

Page 8: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

60 Anti-Collagen I antibody [COL-1] (ab90395) 50 µl 01 Pack

61 Phusion High-Fidelity DNA Polymerase (2 U/µL)

Catalog number: F530S 05 Pack

62 Kanamycin 01 Pack

63 Pipetters 25ml disposable 200/case 02 Case

64 Streptozotocin 1G/pk 5 Vials

65 Multichannel Pippetman 100µl 01 No.

66 Multichannel Pippetman 1000µl 01 No.

67 Digital Pippetman 100µl 01 No.

68 Digital Pippetman 1000µl 01 No.

69 Ladder 10bp Cat:SM1313 01 Pack

70 RNase Erase Catalog Number: 821682 01 No.

71 Flask 4L Base 20.6cm Height 36cm 12 Nos.

72 Flask 4L Base 21cm Height 36cm Baffeled 12 Nos.

73 RNA Store Reagent 04 Nos.

Page 9: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

74

Hematoxylin solution modified acc. to Gill II;

Synonym: Hematoxylin solution acc. to

GILL, Hematoxylin solution acc. to ... 500 ml in Plastic

bottle (1051750

01 Pack

75

EnVision™ FLEX Mini Kit, High pH,

(Dako Autostainer/Autostainer Plus) ..... FLEX DAB+

Chromogen (DM827), una solución de

diaminobencidina (DAB)

01 Pack

76

EnVision FLEX, High pH is a high-sensitivity

visualization system intended for use in

... EnVision/HRP, DAB+ Chromogen, Substrate Buffer,

Target Retrieval …DM 823

01 Pack

77 DPX 1005790500 01 Pack

78 Anti mouse serum NS03L-1ML Sigma 01 Pack

79 Histopaqueâ -1077 Catalog Number 10771 Storage

Temperature 2–8 °C 500ml 01 Pack

80

Gibco™ RPMI 1640 Medium, Powder Non-distribution

product as customer accommodation. Available on ... as

a monolayer. Manufacturer: Gibco™ 31800022

01 Pack

81 EVOS™ Light Cube, RFP Cat:AMEP4652 01 Nos.

82 MP Biomedicals™ Collagenase Type I

from Clostridium histolyticum1gm 02 Pack

Page 10: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

83

H: Science Lab Microcentrifuge Tube Racks:

Amazon.com: Industrial & Scientific. ... Part

Number, 03-448-20 ... Tube Rack, Fisherbrand; Slip-

Fit Top; 96-Well; 5 L x 4 W x 1-1/2 in H; Revesible;

Std 8x12 Array; Snap-In-Place Lid; Easy-To-Grasp

pack of 5

04 Pack

84 Eco31I (BsaI) (10 U/µL) ER0291 1000U 02 Vials

85 Streptavidin, Alkaline Phosphatase Conjugate S921

0.5ml 02 Vials

86 Anti-HA tag antibody [HA.C5] (ab18181) 01 Pack

87 QIAquick Spin Columns (100) Cat No./ID: 28115 02 Pack.

88 QIAquick PCR & Gel Cleanup Kit (100) Cat No./ID:

28506 01 Pack

89 Phusion High-Fidelity DNA Polymerase (2 U/µL)

Catalog number: F530S 500U 02 Pack

90 NanoDrop™ Lite Spectrophotometer ND-LITE 01 No.

91 RNAlater™ Stabilization Solution Cat:AM7020 100ml 01 Pack

92 DNA Size Standard 550 (BTO) for 100 reactions Cat

No./ID: 386015 03 Pack

93 Investigator DIPplex Kit (100) Cat No./ID: 384015 02 Pack

94 Investigator 24plex QS Kit (100) Cat No./ID: 382415 01 Pack

Page 11: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

95 DNA Size Standard 24-plex Cat No./ID: 386037 01 Pack

96 Matrix Standard BT6 (50) Cat No./ID: 386224 01 Pack

97 Buccal Swabs 500 Nos.

98 Spatula 05 Nos.

99 Tetracosane CAS # 646-31-1 01 Pack

100 Diazomethane CH2N2 01 Pack

101 Acetic anhydride (Ac2O) 01 Pack

102 TRIzol Reagent 15596-026 100ml 01 Btl.

103 Thermpmeter thermo work

Fridge Freezer RT801 06 Nos.

104 Nunc Easy Flask 75cm2 Case of 100 156499 02 Case

105 Direct-zol RNA Miniprep Plus (Product Supplied w/ 50

ml TRI Reagent) R2071 01 Kit

106 dNTP Set (100 mM) Cat: 10297018 4 x 250 µL 10 Pack

107 HCV Quantative Realtime PCR Kit 100/2 FRT Sacace 01 Pack

108 LongAmp® Taq DNA Polymerase Cat:M0323S 2,500

units/ml 500 units 03 Pack

Page 12: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

109 Pfu DNA Polymerase 10X Reaction Buffer with

MgSO4 (M776A): 200mM Tris-HCl ep0501 01 Pack

110

Ammonium heptamolybdate tetrahydrate, Ammonium

molybdate tetrahydrate, Molybdic acid ammonium salt

tetrahydrate 12054-85-2

01 Pack

111 Sodium selenite 214485-100G 01 Pack

112 Ferric Chloride 100gm 01 Pack

113 Cupric Chloride dihydrate 200gm 01 Pack

114 SAPI Cat:FD0934 03 Pack

115 SPHI Cat:FD0604 03 Pack

116 Nunc Easy Flask 75CM² Case of 100 156499 05 Case

117 PCR Tubes flat cap 02 Box

118 Gene Synthesis Macrogen 1 Job

Assignments

119 Repair Tube WellLAAR MultanDismentling of Turbine

SystemRepairing & Re-Fixing of Rods 01 Job.

120 Repair Hybirdizer 01 Job.

121 Freez Dryer Repair & Installation 01 No

122 Repair CO² Incubator Binder 01 Job.

123 Work Stations as per sample 03 Nos.

Page 13: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

124 Repair of exhaust of biosafety cabinet

( Ventilation System ) 1 No.

125 Electronic Board Repair Avanti JHC Centrifuge 1 Unit

126 Repair of Ultra Low Freezer 01 Job.

Annual Contract for the year 2020

127 Genes 1501-2000 Annual Coract for the year 2020 Per Base

128 Genes 2001-3000 Annual Coract for the year 2020 Per Base

129 Genes 3001-4000 Annual Coract for the year 2020 Per Base

130 Genes 4001-5000 Annual Coract for the year 2020 Per Base

131 Genes 5001-6000 Annual Coract for the year 2020 Per Base

132 Genes 6001-7000 Annual Coract for the year 2020 Per Base

Annual Contract for the year 2020

133 Primers 25nMole Annual Coract for the year 2020 Per Base

134 Primers 50nMole Annual Coract for the year 2020 Per Base

135 Primers 100nMole Annual Coract for the year 2020 Per Base

136 Primers 200nMole Annual Coract for the year 2020 Per Base

Uniform

137 Required for Secirity Guards 05. Set

Field Jacket Required for Secirity Guards 05.Nos.

Socks Required for Secirity Guards 05. Pair

Gloves Required for Secirity Guards 05. Pair

Jarsee Required for Secirity Guards 05. Nos.

Uniform Required for Secirity Guard 01. No.

IFS Grant I-I-C-6257-1 ( AO 9532 )

138 ThermoPrime Taq DNA Polymerase catalogue #

AB0301B Thermo fisher or equivalent 5 × 2500 units

Pack Size

Brand

Page 14: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

139 AmpliTaq™ DNA Polymerase with Buffer II catalogue

# N8080153 ABI or equivalent 5 × 3000 units

140

Verso 1-Step RT-PCR Kit ReddyMix, with

ThermoPrime Taq catalogue # AB1454LDB Thermo

fisher or equivalent

5 × 200 Rxn

141 Phire Green Hot Start II PCR Master Mix catalogue #

F126L Thermo fisher or equivalent 5 × 1000 Rxn

142 Taq DNA Polymerase, recombinant (5 U/µL) catalogue

# EP0405 Thermo fisher or equivalent

15 × 500

Units

143 Platinum™ Hot Start PCR Master Mix (2X) catalogue

#13000013 Thermo fisher or equivalent 5 × 200 Rxn

144 Maxima SYBR Green/ROX qPCR Master Mix (2X)

catalogue # K0222 Thermo fisher or equivalent 10 × 200 Rxn

145 Power SYBR™ Green PCR Master Mix catalogue #

4368577 Thermo fisher or equivalent 5 × 1 ml

146 TRIzol™ Reagent catalogue #15596018 Invitrogen or

equivalent 5 × 200 ml

147 Biotin DecaLabel DNA Labeling Kit catalogue # K0652

Thermo fisher or equivalent 5 × 30 rxn

148 Biotin Chromogenic Detection Kit catalogue # K0662

Thermo fisher or equivalent 5 × 30 rxn

149 Murashige & Skoog – Plant Tissue Culture media

Phytochemicals or equivalent 1000 litres

Page 15: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

150 TopVision Agarose catalogue # R0492 Thermo fisher

or equivalent 10 × 500 gm

151 DNase I, RNase-free (1 U/µL). Catalogue # EN0521

Thermo fisher or equivalent

10 × 1000

units

152 RNase I (10 U/µL), Cat # EN0601 Thermo fisher or

equivalent

10 × 5000

units

153 First Strand cDNA Synthesis Kit Catalogue # K1612

Thermo fisher or equivalent 5 × 100 rxn

154 GeneJET Plasmid Miniprep Kit Catalogue # K0503

Thermo fisher or equivalent 13 × 250 preps

155 MEGAscript™ T7 Transcription Kit cat # AM13345

Thermo fisher or equivalent 5 × 200 rxn

156 Rapid DNA Ligation Kit Catalogue # K1422 Thermo

fisher or equivalent 10 × 50 rxn

157 GeneRuler Ultra Low Range DNA Ladder Catalogue #

SM1192 Thermo fisher or equivalent 15 × 50 µg

158 GeneRuler 50 bp DNA Ladder Catalogue # SM0372

Thermo fisher or equivalent 15 × 50 µg

159 GeneRuler 100 bp DNA Ladder Catalogue # SM0242

Thermo fisher or equivalent 15 × 50 µg

160 GeneRuler 1 kb DNA Ladder Catalogue # SM0311

Thermo fisher or equivalent 15 × 50 µg

Page 16: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

161 Orange DNA Loading Dye (6X) cat # R0631 Thermo

fisher or equivalent 20 × 1.0 mL

162 ONE-HOUR WesternTM Standard Kit Using TMB

(Rabbit) Catalogue # L00204 Genescript or equivalent 3 × 5 assays

163 Pipetman P variable volume type P10 1-10 µl single

channel Gilson or equivalent 3

164 Pipetman P variable volume type P100 20-100 µl single

channel Gilson or equivalent 3

165 Pipetman P variable volume type P1000 200-1000 µl

single channel Gilson or equivalent 3

166 Pipetman P variable volume type P1000 0.2-2 µl single

channel Gilson or equivalent 3

167 Pipetman P variable volume type P1000 2-20 µl single

channel Gilson or equivalent 3

PARB Project 1075

168 Fast Digest BamH1 (Fermentas) 15

Pack Size

Brand

169 Fast Digest EcoR1 (Fermentas) 10

170 Acetyl L Cartinine (100 g) 2

171 Thermo Taq Polymerase EP-0402/B 40

172 RNAse A (Thermo Scientific) 12091039 10

Page 17: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

173 TURBO™ DNase (2 U/µL) AM2238 1

174 Rapid DNA Ligation Kit (Fermentas) K1423 5

175 CloneJet PCR Cloning Kit (Fermentas) K1232 5

176 pGEM-T Easy Vector System (Promega- A1360) 10

177 DIG DNA Labelling and Detection Kit (Roche-

11093657910) 5

178 UltraPure 1M Tris-Hcl, pH 8.0 (1L Invitrogen- 15568-

025) 2

179 Ultrapure 0.5M EDTA, pH 8.0 (4x100ml - 15575-020) 3

180 Ruthenium Red- R2751-1G 1

181 Sodium Acetate, 3M, sterile, pH 4.5 (200 ml bioplus -

419200241-1 (700890)) 3

182 Lithium chloride-L9650 2

183 Protein Purification Kit (Qiagen-31314) 2

184 Label IT Fluorescence In Situ Hybridization Kit (Mirus

-MIR6510) 5

185 Platinum™ SuperFi™ DNA Polymerase - 12351010 2

Page 18: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

186 pCAMBIA 1305 1

187 pCAMBIA 1301 1

188 Trypton (Merk-JH2JF62250) 2 PACKS

189 Yeast Extract (Merk- JE2JF62125) 2 PACKS

190 Peptone (Merck) 2

191 Blunt/TA Ligase Master Mix (M0367S) 2

192 Sucrose (Omnipure - 8550) 1

193 Agar (Merk-VM014514829) 2 Packs

194 Nuclease-Free Water (129114) 5

195 Tris Base (MP Biomedicals - 11TRIS05KG) 2

196 Acetic Acid (Fisher Scientific) 3

197 Glycerol (Fisher Scientific) 3

198 ABA (Sigma Aldrich- 12886-5GM) 1

199 PEG (Sigma Aldrich - A1049-250 MG) 1

Page 19: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

200 POLYVINYLPYRROLIDONE (02194017) 5

201 GA3 (Sigma Aldrich - G7645-5G) 1

202 BAP (Sigma Aldrich - B3408-5G) 1

203 K3Fe(CN)6 (Sigma Aldrich- 244023-100G-D) 1

204 K4Fe(CN)6 (Sigma Aldrich- 455989-100G) 1

205 NaH2PO4 (Sigma Aldrich- S3139-500G-D) 1

206 TRIzol™ Reagent 15596026 4

207 Glacial Acetic Scid 2

208 mirVana miRNA isolation kit with phenol -AM 1560 1

209 Kanamycine - (Sigma Aldrich) 1

210 Tetracycline - (Sigma Aldrich) 1

211 Riffampicillin - (Sigma Aldrich) 2

212 CefaToxime - (Sigma Aldrich) 2

213 Eriochrome cyanine R (C.I. 43820) - 1031640025 1

Page 20: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

214 Amersham Protran 0.45 NC ( GE Health Care Life

Science-10600002) 2 Roll

215 Hybond N (Amersham Biosciences RPN303N) 2 Roll

216 MES (Sigma- 50g) 2

217 Platinum™ Hot Start PCR Master Mix (2X) 100 react.

13000013 2

218 AcetoSyringone (Sigma Aldrich-D134406-5G) 3

219 Qualiplate for detection PAT/BAR in Corn (Envirologix

- AP-013) 3

220 Qualiplate for detection VIP3A in Corn (Envirologix) 3

221 QuickStix for Cry3A in Corn (Envirologix) 4

222 Quickstix for PAT/AR in Corn (Envirologix) 3

IFS Grant ( 1-3-C-6 19.1-1 )

223 cDNA synthesis Kit K1622 10 Nos.

Pack Size

Brand

224 Fast Digest Xbal FD0985 10 Nos.

225 Trizol Reagent 15596026 10 Nos.

226 Rapid ligation kit K1423 10 Nos.

Page 21: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

227 Genejet plasmid miniprep kit K0503 10 Nos.

228 Genejet PCR purification kit K0702 10 Nos.

229 Genejet extraction kit K 0692 10 Nos.

230 Fast Digest Xhol FD0695 10 Nos.

231 Fast Digest Hind-III FD0505 10 Nos.

232 Digital Block Heater

three sub unit blocks Cat No:88870005 02 Nos.

01.Mixed Block for 18x1.5ml + 10x2.0ml tubes

specifications ( Cat No:88870113 02. Nos

02. Conical bottom block of 4x50ml block specification

( Cat No:88870119) 02 Nos.

03. Conical bottom block for 15x15ml tubes Cat

No:88870118 02 Nos.

Project No.7663 Year 2019

233 Cryovials 2ml Sterile ( 500/pack) Nalgene V5007 5 packs

Pack Size

Brand

234 Rapid Ligation Kit Thermo Scientific K1423 3 kits

235 Quick start Bradford protein assay kit 1 Biorad 5000201 1 bottel

236 ECL PLus Western Blotting Reagent Pack

GE Healthcare RPN2124 2 Kits

237 Ammonium hepta molybdate Merck 101181 1 Pack

238 Ferric chloride hexahydrate Sigma F2877 1 Pack

Page 22: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

239 Sodium selenite Sigma 214485 1 Pack

240 Cupric Chloride Dihydrate Sigma 467847 1 Pack

241 Phenol Solution Sigma Aldrich P4557 1 bottel

242 Phenyl methyl sulfonyl fluride (PMSF) Sigma Aldrich

P7626 1 Pack

243 Hydrochloric Acid Sigma Aldrich H1758 1 bottel

244 SigmaFast – BCIP/NBT tablets Sigma Aldrich B5655 10 pks

245 Mouse Anti-6X His tag® antibody [HIS.H8] abcam

ab18184 2 vails

246 Rabbit Anti-HA tag antibody - ChIP Grade abcam

ab9110 2 vails

247 Mouse Anti-HA tag antibody [4C12] abcam ab1424 2 vails

248 Goat Anti-Rabbit IgG H&L (HRP) abcam ab205718 2 vails

249 Goat Anti-Mouse IgG H&L (Alkaline Phosphatase)

abcam ab6790 2 vails

250 Goat Anti-Mouse IgG H&L (HRP) abcam ab205719 2 vails

251 Goat Anti-Mouse IgG H&L (HRP) preadsorbed abcam

ab97040 1 vail

Page 23: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

252 Amersham ECL Mouse IgG, HRP-linked whole Ab

(from sheep) Amersham NXA931 2 vails

253 Hypercassette™ Autoradiography Cassette

Hypercassette 30 × 40 cm Amersham 10127064 1 cassette

254 Nitrocellouse membrane Amersham 10600002 1 pack

255 Tetracycline Hydrochloride Antibiotic Fisher Scientific 1 bottel

256 Pyrex Reagent bottles 500ml ( 10/cs) Fisher Scientific

06-414-1F 3 case

257 Ultra-Pure Tris Base -1kg Invitrogen 15504-020 3 Pack

258 Gene Art Cryopreservation Kit for Algae - Invitrogen

A24228 1 kit

259 Sealing film IWAKI 1804-096 2 paksa

260 ELISA Plate 96 well flat bottom IWAKI 3801-096 5 cases

261 Amicon Ultra-0.5 Centrifugal Filter Unit (3 Kda) Merck

UFC500396 1 pk (96/pk)

262 Amicon Ultra-0.5 Centrifugal Filter Unit (10 Kda)

Merck UFC501096 2 pk (96/pk)

263 Amicon Ultra-0.5 Centrifugal Filter Unit (30 Kda)

Merck UFC503096 2 pk (96/pk)

Page 24: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

264 Amicon Ultra-0.5 Centrifugal Filter Unit (50 Kda)

Merck UFC505096 1 pk (96/pk)

265 Amicon Ultra-0.5 Centrifugal Filter Unit (100 Kda)

Merck UFC510096 1 pk (96/pk)

266 Amicon® Ultra-15 Centrifugal Filter Unit ( 5 kda)

Merck UFC900396 1 pk (96/pk)

267 Amicon® Ultra-15 Centrifugal Filter Unit ( 10 kda)

Merck UFC901096 1 pk (96/pk)

268 Amicon® Ultra-15 Centrifugal Filter Unit ( 30 kda)

Merck UFC903096 1 pk (96/pk)

269 Amicon® Ultra-15 Centrifugal Filter Unit ( 50 kda)

Merck UFC905024 2 pk (24/pk)

270 Amicon® Ultra-15 Centrifugal Filter Unit ( 100 kda)

Merck UFC910024 3 pk (24/pk)

271

Steritop-GV 0.22um Durapore (PVDF) membrane,

radio-sterilized, 45mm 500mL /PK 12 Millipore

MILSCGVT05RE

10 Packs

272 cOmplete™, EDTA-free Protease Inhibitor Cocktail

ROCH 5056489001 1pack ( 60 ,pk)

273 LabTotal Vial Certified Kit for LC/LCMS (100 pcs)

SHIMADZU 227-34001-01

2 pack

(100vails)

274 Glycine Powder -1kg Sigma G8898 5 Packs

275 Brilliant Blue G-250 sigma 1.15444 2 Packs

Page 25: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

276 Acetic Acid Sigma A6283 1 bottel

277 Triton-X 100 Sigma T8787 1 bottel

278 Tween-20 sigma P9416 1 bottel

279 Colli-Rollers-Plating Beads Sigma 71013 2 rollers

280 Bromophenol BlueSodium salt Sigma B8026 1 Pack

281 Manganese (II) chloride tetrahydrate Sigma 203734 1 Pack

282 Sodium Citrate Saline SSC 20X buffer Sigma S6639 1 Pack

283 Absolute Ethanol laboratory reagent Sigma 32205 1 bottel

284 Sodium Acetate Anhydrous Sigma Aldrich W302406 1 Pack

285 Glycerol 99% Sigma Aldrich G5516 1 bottel

286 Protease Inhibitor Cocktail Sigma Aldrich S8830 1

287 SigmaFast 3,3’-Diaminibenzidine tablets Sigma Aldrich

D4293 10 pks

288 Methanol laboratory reagent Sigma Aldrich 34860 1 bottel

289 Carbenicillin, disodium salt Antibiotic Thermo

10177012 1 bottel

Page 26: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

290 Blotting Papers Thermo Scientific LC2010 3 paks

291 Zeocin Antibiotic Thermofisher R25005 1 bottel

292 Cryoboxes 25 ( 1-1.8 ml vails ) case of 24 Thermofisher

378247 5 cases

293 Blotting Roller Thermofisher LC2100 2 packs

294 Syring Disposable filters 0.22µm 1 Pack

295 Spectophometer glass cuvettes 2 Pack

296 Glass Beaker 250ml, 10 Nos.

297 Incubation Tray for Western blots thermoscientific

LC2102 10 Packs

298 Baffled flasks 2000 ml 10

299 Baffled flasks 1000 ml 10

300 HiPrep IMAC FF 16/10 (Dr shazia) 17-0921-06 2X20 ml

301 HiLoad 26/10 SP Sepharose HP 17-1138-01 2X53 ml

302 Phosphatase Substrate Kit thermoscientific 37620 1 kit

303 Diethanolamine Substrate Buffer (5X) thermoscientific

34064 1 kit

Page 27: Tender Ref:- CEMB NP 21- 2019

National Centre of Excellence in Molecular Biology University of the Punjab

304 Pierce™ TMB Substrate Kit thermoscientific 34021 2 pks

305 1-Step™ ABTS Substrate Solution thermoscientific

37615 2 pks

Pack Size

Brand