TEIN 4:RNA-seq analysisAkzam Saidin

Sr. Bioinformatics Scientist

RNA Transcription and Processing

Gene Structure and Alternative Splicing

2015 NOVOCRAFT TECHNOLOGIES. PROPRIETARY AND CONFIDENTIAL.

Alignment Accuracy

Trial 1 Trial 2

Figure from Wang et. al, RNA-Seq: a revolutionary tool for transcriptomics, Nat. Rev. Genetics 10, 57-63, 2009.

2015 NOVOCRAFT TECHNOLOGIES. PROPRIETARY AND CONFIDENTIAL.

2015 NOVOCRAFT TECHNOLOGIES. PROPRIETARY AND CONFIDENTIAL.

2015 NOVOCRAFT TECHNOLOGIES. PROPRIETARY AND CONFIDENTIAL.

References 1. KR Kukurba and SB Montgomery. RNA Sequencing and Analysis. 2015 Cold Spring Harb Protoc; doi:10.1101/pdb.top084970

2. M Griffith , JR Walker, NC Spies, BJ Ainscough, OL Griffith. Informatics for RNA Sequencing: A Web Resource for Analysis on the Cloud. 2015 PLOS Computational Biology. DOI: 10.1371/journal.pcbi.1004393

3. Z Wang, M Gerstein and M Snyder. RNA-Seq: a revolutionary tool for transcriptomics. 2009 Nat Rev Genet. 10(1):57-63. doi: 10.1038/nrg2484