TECHNOTHROMBIN® TGA

Fluorogenic Thrombin Generation Assay

For Research Use Only

ML-00-00110 Rev01

FOR RESEARCH USE ONLY

QUANTITATIVE MEASUREMENT OF THROMBIN GENERATION

Time

Inactivation phase

Peak Thrombin

Lag Phase

Slo

pe

TEST PRINCIPLE For Research Use Only

The use of TECHNOTHROMBIN® TGA for such

diverse applications is made possible because

TECHNOTHROMBIN® TGA measures the whole kinet-

ics of thrombin generation not only during the initiation

phase of thrombin formation with the end point fibrin

formation, but also during the phase of down regula-

tion of thrombin formation and inactivation of the

formed thrombin. TECHNOTHROMBIN® TGA is there-

fore an universal tool to be used in the research of the hemostatic system.

Küvette

LED

Photodiode

Measuring principal of TGA modules

The new automated analyser CEVERON® alpha with a TGA module is equipped with a fluorescence

measurement module and thus suitable for assays such as TECHNOTHROMBIN® TGA. With

CEVERON® alpha, standard clotting tests and thrombin generation assays can be performed.

Thrombin Generation is measured with a special adapted TGA fluorimetric module which is placed over the cuvette rotor. With an UV emitter (360 nm) placed in the module, thrombin generation can then be measured.

Alternatively, this assay can be performed manually and measured using a flu-orescence reader. Applications and eva-luation software are available for the most established readers.

Th

rom

bin

TECHNOTHROMBIN® TGA is a Thrombin Generation Assay (TGA) based on monitoring the formation

of thrombin by means of a fluorogenic substrate upon activation of the coagulation cascade by tissue

factor. This assay can be used to research thrombin generation in bleeding and clotting studies.

ML-00-00110 Rev01

FOR RESEARCH USE ONLY

Application dependent reconstitution of thrombin generation potential in Factor VIII inhibitor plasma after addition of FEIBA

(Factor Eight Inhibitor Bypassing Activity)or rFVIIa.

TECHNOTHROMBIN TGA RC LOW/ rVIIa

FVIII INH 3O31000 [58.4 BU/ml]

0

20

40

60

80

100

120

140

0 20 40 60 80 100 120 140

time (min)

nM T

hrom

bin

300E / 300U 150E / 150U 75E / 75U 37,5E / 37,5U

18,8E / 18,8U 9,4E / 9,4U 4,7E / 4,7U 2,3E / 2,3U

1,2E / 1,2U 0,59E / 0,59U 0,29E / 0,29U 0,15E / 0,15U

0,073E /0,73U

TECHNOTHROMBIN® TGA RD / HEPARIN

0,00

100,00

200,00

300,00

400,00

500,00

600,00

0 10 20 30 40 50 60

time [min]n

M T

hro

mb

in

0U/ml RD 8U/ml RD 4U/ml RD 2U/ml RD 1U/ml RD

Application dependent decrease of thrombin

generation potential in plasma after addition of

different heparin or direct Xa inhibitor concentrations.

THROMBIN GENERATION BY MICRO PARTICLES

Thrombin generation induced by micro particles can be measured by the TECHNOTHROMBIN

® TGA assay.

The amount of thrombin generated is dependent on the number of micro particles present in the sample.

FVIII INHIBITOR ANALYSIS HEPARIN ANALYSIS

ML-00-00110 Rev01

FOR RESEARCH USE ONLY

Reagent Purpose

TGA RA - to study the activity of circulating micro particles contained in platelet poor plasma

TGA RB, RC

Low

- research in thrombophilia tendency and FVIII inhibitors

TGA RC High - anticoagulation studies

TGA RD - heparin studies, direct thrombin and Xa inhibitor research

TECHNOTHROMBIN TGA TRIGGER

Technoclone GmbH

Brunner Str. 67 I 1230 Vienna I Austria

Tel: +43 1 86 373-0 I Fax: +43 1 86373-44

sales@technoclone.com I www.technoclone.com

TG

A001D

.01

4 0

6/2

013

ADDITIONAL AVAILABLE PRODUCTS:

Modular reagents

REF 5006205 TECHNOTHROMBIN® TGA RA 5 x 0.5 mL

REF 5006206 TECHNOTHROMBIN® TGA RA 50 x 0.5 mL

REF 5006209 TECHNOTHROMBIN® TGA RB 5 x 0.5 mL

REF 5006210 TECHNOTHROMBIN® TGA RB 50 x 0.5 mL

REF 5006212 TECHNOTHROMBIN® TGA RC Low 5 x 0.5 mL

REF 5006213 TECHNOTHROMBIN® TGA RC Low 50 x 0.5 mL

REF 5006214 TECHNOTHROMBIN® TGA RC High 5 x 0.5 mL

REF 5006216 TECHNOTHROMBIN® TGA RC High 50 x 0.5 mL

REF 5006220 TECHNOTHROMBIN® TGA RD 5 x 1.5 mL

REF 5006222 TECHNOTHROMBIN® TGA RD 50 x 1.5 mL

REF 5006235 TECHNOTHROMBIN® TGA SUB 5 x 1.5 mL

REF 5006230 TECHNOTHROMBIN® TGA SUB 50 x 1.5 mL

Additional Controls

REF 5006320 TECHNOTHROMBIN® TGA Control High 5 x 1 mL

REF 5006330 TECHNOTHROMBIN® TGA Control Low 5 x 1 mL

REF 5006345 TECHNOTHROMBIN® TGA CAL Set 1 Set

For T

GA

Reage

nts

for C

evero

n® a

lph

a s

ee s

epara

te brochure.

REF 5006010 TECHNOTHROMBIN® TGA Kit 3x16T.

ML-00-00110 Rev01

FOR RESEARCH USE ONLY