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Technical bulletin no. 5
Experimental animals 1 day old broiler chickens Babolna Hybrid
Diets Drug-free, high-protein content diet (25%). No coccidiostat nor AGP added
Infection protocol Simulation of Subclinical Necrotic Enteritis (NE) following Gholiamiandehkordy methodology
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© Norel, S.A. All rights reserved. CIF: A-28617801 · C/Jesús Aprendiz, 19 1º A y B, 28007 Madrid · SPAIN +34 915 014 041 · www.norel.net · [email protected]
Age of chickens (days) 1 16 17 18 19 20 21
Gumboro Vaccine (CEVAC GUMBL L®, Ceva-phylaxia) Induce immunesupresion
Artificial infection with clostridia (6-8 x 108 CFU 3 times/day)Toxigenic bacteria
Overdose (10x) of coccidial vaccine (Paracox-5®, Ceva-phylaxia) Damage in intestinal mucosa
Group Animals Additive (g/kg feed)
Paracox-5®
VaccineGumboro®
Vaccine
Positive Control 10 - + +
BP70® infected 10 1.5 g/kg + +
NATESSE® infected 10 1.5 g/kg + +
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Technical bulletin no. 05
Table 2. Experimental design. Pathological and hystopathological evaluation
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© Norel, S.A. All rights reserved. CIF: A-28617801 · C/Jesús Aprendiz, 19 1º A y B, 28007 Madrid · SPAIN +34 915 014 041 · www.norel.net · [email protected]
Group Animals Additive (g/kg feed)
Paracox-5®
VaccineGumboro®
VaccineC. perfringens
A
Negative Control
Positive Control 10 - + +
BP70® non-infected
NATESSE® non-infected
BP70® infected 10 1.5 g/kg + +
NATESSE® infected 10 1.5 g/kg + +
2
0
2
4
6
8
Control GUSTOR BP70® NATESSE®
Scores
Pathological Histopathological
Technical bulletin no. 05
3© Norel, S.A. All rights reserved. CIF: A-28617801 · C/Jesús Aprendiz, 19 1º A y B, 28007 Madrid · SPAIN +34 915 014 041 · www.norel.net · [email protected]
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250
350
450
550
650
Control Gustor BP70 Natesse
Body weight (g)
17 days (before infection) 23 days (after infection)
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Scores Control GUSTOR BP70® NATESSE®
Pathological 3 ± 1 3.1 ± 1.6 0.8 ± 0.9
Histopathological 3.7 ± 1.7 3.6 ± 1.9 2 ± 1.1
Group IL-1β IL-2 IL-8 IL-10 CD3 LITAF TNFSF15
BP70® non-infected
NATESSE® non-infected UP UP UP UP DOWN
BP70® infected UP DOWN
NATESSE® infected UP DOWN
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© Norel, S.A. All rights reserved. CIF: A-28617801 · C/Jesús Aprendiz, 19 1º A y B, 28007 Madrid · SPAIN +34 915 014 041 · www.norel.net · [email protected]
Technical bulletin no. 05
4© Norel, S.A. All rights reserved. CIF: A-28617801 · C/Jesús Aprendiz, 19 1º A y B, 28007 Madrid · SPAIN +34 915 014 041 · www.norel.net · [email protected]
