Characterization of drug release profile of glutathione filled gelatin-B microspheres

embedded in ovalbumin-scaffoldsTeam Members: Adwoa Baah-Dwomoh, Annie Ellis, Chandler Slate,

and Nathaneal MayAdvisor: Dr. Abby Morgan

The goal of this project is to determine the effect of embedding glutathione-impregnated microspheres into an ovalbumin scaffold on the drug-release profile of the microspheres. These drug-delivery devices will simulate some functions of natural body tissue, and will be able to deliver drugs in a much more controlled way than currently possible. In doing so, they will provide doctors with new ways to treat bone disease.

The first phase of this project involves perfecting the fabrication method for producing microsphere particles. Using this same method, about twelve grams of gelatin microspheres are made using an water in oil emulsion technique. To ensure consistency and accuracy, the particle size distribution of the microspheres is examined using optical microscopy. The next phase is to perfect the procedure for creating ovalbumin scaffolds. This procedure includes the addition of drug-filled microspheres to the scaffold. Using confocal, SEM, and optical microscopy the microsphere particle distribution within the ovalbumin scaffold will prove consistency. The next stage is to embed the drug in the gelatin microspheres. Glutathione, the chosen dug, is an antioxidant. Finally, the drug filled microspheres are evenly distributed throughout the ovalbumin scaffold. The drug release profile is then completed and analyzed using UV spectroscopy.

Team Photo Here

Project Image Here