Tables and sources 23-11-2007-3-

Prepared by: Saleh Mutahar Yahya Al-Othrubi UMBI Updatedon 27/10/2008 Sat: 13/09/2008في معدلة ألخرى للرجوع 8/8/2008بحاجة

Table 1.1: showing the suspected V. parahaemolyticus isolated from shrimp between June 2004 and May 2005, obtained from Prof. Dr. Son Radu, recovered from storage isolates on LB slant agar medium stored at room temperature.

No. Date Location Sample no. Isolate code no. G on TCBS G on CV toxR tlh tdh trh

1 28/6/04 Parit Buntar, Perak Shrimp VP 5s + +













14 28/6/04 Parit Buntar, Perak Shrimp VP25s + +












*g: green colour, m: mauve colour

1


Table 1.2: shows the suspected V. parahaemolyticus isolated from seawater between June 2004 and May 2005, obtained from Prof. Dr. Son Radu, recovered from storage isolates on LB slant agar medium stored at room temperature.

No. Date Location Source Isolate code no. G on TCBS G on CV toxR tlh tdh trh

1 28/6/04 Parit Buntar, Perak Seawater VP 13s + +



4 28/6/04 Parit Buntar, Perak Seawater VP47s + +





9 28/6/04 Parit Buntar, Perak Seawater VP 29s y + +











20 22/7/04 Nibong Tebal, Penang Seawater VP 91s y + +


2


Table 1.3: showing the suspected V. parahaemolyticus isolated from cockles collected between isolated March 2005 to Feb 2006 optained from Prof. Son Radu, recovered from storage isolates at -20°C in LB broth supplemented with 15% Glycerol.

No. Date Location Sources Isolate Code Growth on TCBS

Growth on CV

toxR tlh tdh trh

1 4/7/05 Pasar borong Selangor(Stall 1) Cockles VP52s + +








9 11/8/2005 Pasar malam Sri Serdang(Stall 2) Cockles VP69s + +








17 11/8/05 Gaint Cockles VP84s + +

18 11/8/05 Taikaly Cockles VP87s + +



21 11/8/05 Kedai Runcit Zakaria Cockles VP79s + +





3






















Table 1.4: Represents Clinical strains isolated from patients, obtained from different sources in Malaysia; local Hospitals, Medical health Centres and others and stored at -80°C on beads.Code Source/patients Clinical Isolates Strain code G on TCBS G on CV toxR tdh trh tlh

2s HUKM V. vulnivicus VvUKM + colorless + - - -

HUKM-Enshurance Pts V. vulnivicus VvUKM + colorless + - - -

HUKM V. parahaemolyticus T41066 P/P* + + + + - +

UM* V. parahaemolyticus VP-UM + + + + - +

USM** V. parahaemolyticus VP-J1206 + + + + - +

* UM: UM – Clinical Microbiology, supported ** USM: USM; Clinical Microbiology, supported by Dr. *** IPL: International public Laboratory, supported by Dr. Patrick

4


HUKM- Dr Alfizah, P/P*: T 41066 VP Gurmeet Kaur 32/F (Sirgh-Indian),Feces, Wad Nilam (8/8Acute GI 7/8/06); Cilic: G.P 2+1 , 34+2/7(34 week pregnance, 2 days gastrointeritis/7 days G.P 2+1 after delivary 2 alife + (one) died

Table 1.5: showing the Reference strains of V. parahaemolyticus obtained from Prof. Dr. Son Radu, UPM) stored at room temperature on LB slant agar medium.Code Source Reference Strains Strain no. G TCBS G on CV toxR tdh trh tlh

4s Japan V. parahaemolyticus VP 2053 + + + + - +

11s Japan V. parahaemolyticus VP 1808 + + + + - +

3s Japan V. parahaemolyticus VP1896 + + + - + +

1s Japan V. alginolyticus V. algino2341 +Wt opaque + incolore - - - +

ATCC Japan Escherichia coli ATCC 25922 - - - - - -

See File Doc. –2, P:6

Table 1.6: 37 isolates of V. parahaemolyticus isolated from shrimp between Jouly 2006 and February 2007(obtained from Tunung/master student under supervision of Prof. Dr. Son Radu, UPM) recovered from storage isolates on LB broh medium stored at -20 to -30 ºC.

No Strain code

Strain no. Source LB b3% NaCl

TCBS CV API 20NE toxR tdh trh tlh

1 A VP108sr shrimp + + + Low ID VP + - - +2 2 VP109sr shrimp + + + Low ID VP + - - +3 9 VP110sr shrimp + + + Low ID VP + - - +4 12 VP111sr shrimp + + + Low ID VP + - - +5 14 VP112sr shrimp + + + Low ID VP + - - +

5


6 15 VP113sr shrimp + + + Low ID VP + - - +7 17 VP114sr shrimp + + + Low ID VP + - - +8 18 VP115sr shrimp + + + Low ID VP + - - +9 19 VP116sr shrimp + + + Low ID VP + - - +

10 20 VP117sr shrimp + + + Low ID VP + - - +11 21 VP118sr shrimp + + + Low ID VP + - - +12 22 VP119sr shrimp + + + Low ID VP + - - +13 23 VP120sr shrimp + + + Low ID VP + - - +14 25 VP121sr shrimp + + + Low ID VP + - - +15 28 VP122sr shrimp + + + Low ID VP + - - +16 29 VP123sr shrimp + + + Low ID VP + - - +17 30 VP124sr shrimp + + + Low ID VP + - - +18 31 VP125sr shrimp + + + Low ID VP + - - +19 32 VP126sr shrimp + + + Low ID VP + - - +20 37 VP127sr shrimp + + + Low ID VP + - - +21 40 VP128sr shrimp + + + Low ID VP + - - +22 42 VP129sr shrimp + + + Low ID VP + - - +

* Low ID: 16.5 % because the isolates are not pure. ** DNA was extracted from the LB b inoculated at UPM – Prf Son Lab directly from Tunug samples and PCR for toxR have done from this medium.

Table 1.7: showing the suspected V. parahaemolyticus isolated from Cockles during the MRA in Practice workshop, Auqust 23rd -24th 2007 UPM

No. Date Location SourcesIsolate Code

Isolate no.

Growth on TCBS

Growth on CV

toxR tlh tdh trh

1 23/08/07 Alamanda Market Putra Jaya

Cockles G10/7 VP130sr + +

2 23/08/07 Alamanda Market Cockles G10/6 VP131sr + +

3 23/08/07 Putra Jaya Cockles G8/1 VP132sr + +




6










7

Documents

Tables and sources 23-11-2007-3-