Table of Contents publications/September … · Pat Laturnus President ... Good results were obtained with RSID for undiluted blood and for the 1:10 dilutions as shown in ... This

Table of Contents

2014 IABPA Officers 1

President’s Message 2

Research Article – Effect of Four Latent Blood Visualization

Products on DNA

Céline Nicloux and Jessica Bressler 3

The Annual IABPA Training Conference in Portland, Maine in 2014 12

The Fifth European IABPA Conference in Rome, Italy in 2015 12

Organizational Notices 13

Training Opportunities 14

Editor’s Corner 19

Publication Committee/Associate Editors 20

Past Editors of the IABPA News/Journal of

Bloodstain Pattern Analysis 20

Past Presidents of the IABPA 20

Journal of Bloodstain Pattern Analysis 1 Vol. 30 No. 3 September 2014

2014 IABPA Officers

PRESIDENT

Patrick Laturnus [email protected]

Vice President - Region I Vice President - Region II

Pacific Mountain

Don Schuessler Richard J. Tewes [email protected] [email protected]

Vice President - Region III Vice President, Region IV

Central Eastern

DeWayne Morris Anthony Mangione

[email protected] [email protected]

Vice President - Region V Vice President - Region VI

European Pacific Rim

Martin Eversdijk Ted Silenieks

[email protected] [email protected]

Secretary / Treasurer Sergeant at Arms Norman Reeves Jeffrey Scozzafava [email protected] [email protected]

Historian

Stuart H. James [email protected]

mailto:[email protected]



President’s Message

Hello Everyone,

I appreciate this opportunity to communicate with all of you through our Journal of Bloodstain Pattern Analysis. This format allows for trustworthy internet communication. In a time when we are never sure of misinformation, please remember that articles appearing in this Journal are well worth the read and offer the confidence of peer review backed by our Association.

The 2014 IAPBA Training Conference, hosted by the Maine State Police in Portland Maine, is ready to go. Please review the program and if you haven't registered I'm sure that you'll be inspired to do so afterwards. This is the best opportunity for you to stay current in our discipline. It will also expose you to techniques that you may not have been aware of and will provide a view to improve your own crime scene processing routine.

Our Executive Board will meet on Monday, September 29th. We have a good agenda that will discuss the business of our Association. Please give it some thought, there may be some issue that causes you concern or perhaps something that you want to encourage. Now is a good time to contact myself or your Vice President and you can be sure your ideas will be brought to light.

It's not too early to make plans to attend the 2015 European Conference in Rome, Italy. So many of our members find it difficult to attend the North American Training Conference, this venue allows them the opportunity to share new research as well as techniques and case experiences. It's also a huge occasion for our North American members to be exposed to different approaches. I hope that you are making plans to attend this great event. If you have any questions, please refer to our website and contact the Conference organizer, Dr. Andrea Berti.

At this time of year ( autumn, where we live), is when most of our Court dates are set. As you know, Bloodstain Pattern Analysis has become an integral part of any bloodletting case. Let this Journal help you to prepare. By searching various topics you'll find information that not only brings confidence but helps you to articulate your findings. How you represent Bloodstain Pattern Analysis on the stand, reflects on our entire discipline. It's our hope that you'll be prepared and do us proud. Please use this Journal to full advantage and after you do, consider the article that you can contribute. What you do is important and of interest to all of us.

See you in Portland, Maine; Pat Laturnus President IAPBA


RESEARCH ARTICLE

Effect of Four Latent Blood Visualization Products on DNA Céline Nicloux and Jessica Bressler

Institut de Recherche Criminelle de la Gendarmerie Nationale (IRCGN) Rosny-Sous-Bois,

France

Abstract Bloodstain pattern analysts first document visible stains and patterns at a scene. Then, in order to obtain as much

information as possible from the scene, they search for latent bloodstains and patterns. Latent bloodstains may be the result of old scenes, scenes altered by weather or fire or clean up attempts to destroy evidence. Due to the existence of false positives, analysts must determine if those latent patterns are blood, human blood and then have DNA analysis performed.

Several chemical products are universally utilized to visualize latent bloodstains. Some have a direct and known negative effect on DNA such as o-tolidine, benzidine and TMB (tetramethylbenzidine) while some others can improve both visualization and DNA analysis after sampling. But how “DNA friendly” they are?

In this article, four different products used to visualize latent bloodstains will be compared. The products tested all have luminol-based formulations that contain varying amounts of hydrogen peroxide. Two of the reagents contain fluorescein.

Introduction

The goal of this experiment is to compare the effect/no effect over time of Bluestar® Forensic, Bluestar® Forensic Magnum, Lumiscene and Lumiscene Ultra on DNA analysis. In order to simulate a crime scene in which blood was subjected to clean-up attempts or otherwise altered, blood dilutions from 1 to 1:2000 were tested. As all analysts use a variety of chemical methods and solution strength to visualize latent bloodstains, three different volumes of products (20, 60 and 100 µl) were added to each dilution. For a statistic goal, three samples were prepared for each volume and dilution. There is usually a delay between the collection of the blood sample and the actual DNA analysis. Therefore, DNA analyses were made at D0, D30 and latter at D60. At least 924 swabs prepared and analyzed.

D0 = DNA analysis on first day with chemicals in contact with diluted blood D30 = DNA analysis at 30 days with chemicals in contact with diluted blood D60 = DNA analysis at 60 days with chemicals in contact with diluted blood

In order to complete the study, a second experiment was done to compare two types of

immunochromatographic tests Hemoglobin (Hb) test and Rapid Stain Identification of Human Blood (RSID™) were used to confirm the presence of human blood.

The second test revealed human blood without any false positive results. The tests were performed at the Biology department of the Institut de Recherche Criminelle de la Gendarmerie Nationale (IRCGN) in Rosny-Sous-Bois France. Materials and Methods Blood Collection

Blood was drawn from a known donor into a collection tube containing EDTA. For all experiments, a total of eight dilutions were done. Dilutions were the following: 1:1, 1:10, 1:20, 1:100, 1:200, 1:500, 1:1000 and 1:2000.


Chemical Reagents for Experiments 1 and 2 Bluestar® Forensic, Bluestar® Forensic Magnum Lumiscene Lumiscene Ultra

Procedure Experiment 1: DNA Sensitivity

Each experiment was conducted at D0, D+30 and D+60 periods. The samples were prepared in individual Eppendorf® tubes. Three volumes of chemicals

were tested (20, 60 and 100µL). A volume of 20µL of diluted blood was added to each tube. Three swabs were tested for each volume. For each period of the experiment, negative and positive controls were performed At least 924 swabs were prepared and analysed

The sequential steps are presented in figure 1.

Experiment 2: Human Blood Testing

Each experiment was performed at D0 and D+60 periods For each dilution, one volume of 50µL of chemicals was tested and one volume of 20µL of

blood was used. For each volume, three spots were put on a mat paper. Each paper was dried at room

temperature for 0 and 60 day periods. For each period of experiment, some negative and positive controls were performed Each sample was prepared by placing the blood volume on a mat followed by the blood

chemical volume. A total of 328 spots for both tests (2 × 164) were prepared and analyzed for all periods. Spots were cut and placed in a specific reagent (specific for the test studied) according to

Hemoglobin (Hb) and RSID™ blood testing protocols. For each period of experiment, negative and positive controls were performed.

The sequential steps are presented in Figure 2.

agent agent + blood

blood + agent

(chemiluminescence

reaction)

blood + agent

= solution

(after reaction)

solution drop-

off

Figure 1. Diagram of sequential steps for experiment 1: DNA sensitivity.


Spots of blood spots of blood+agent spot placed in a test

flask Test kit

Figure 2. Sequential steps for experiment 2: Human blood testing.

Results

DNA Sensitivity

Each profile was analysed according to their quality based on a score as shown in Table 1.

Table 1. Scoring system for each DNA profile

0 Complete profile (0 or 1 locus affected)

1 Profile with 1 anomaly: Partial or unbalanced (from 2 loci affected)

2 Profile with 2 anomalies: Partial or unbalanced (from 2 loci affected)

3 Non explicable profile

Results at D0

Negative controls did not reveal any positive results and positive controls revealed positive results for all swab from undiluted blood to 1:500 dilution. The results for Lumiscene (L), Bluestar® Forensic (BS) Bluestar® Forensic Magnum (BM) and Lumiscene Ultra (LU) are presented in Tables 2-6.


Table 2. Results for Lumiscene (L)

Lumiscene

Blood dilutions

Pure 1/10 1/20 1/100 1/200 1/500 1/1000 1/2000

Chemical volumes

(µL)

20

Test 1 0 0 0 0 2 3 2 3

Test 2 0 0 0 0 1 2 3 3

Test 3 0 0 0 0 1 2 2 3

60

Test 1 0 0 0 0 2 3 3 3

Test 2 0 0 0 0 0 2 3 3

Test 3 0 0 0 0 2 2 3 3

100

Test 1 0 0 0 0 2 2 3 3

Test 2 0 0 0 2 2 2 3 3

Test 3 0 0 0 0 2 3 3 3

Table 3. Results for Bluestar® Forensic (BS)

Bluestar

Blood dilutions

Pure 1/10 1/20 1/100 1/200 1/500 1/1000 1/2000

Chemical volumes

(µL)

20

Test 1 0 0 0 1 2 2 2 3

Test 2 0 0 0 2 1 2 2 3

Test 3 0 0 0 0 2 2 2 3

60

Test 1 0 0 0 0 2 2 3 3

Test 2 0 0 0 0 0 3 3 3

Test 3 0 0 0 0 1 2 3 3

100

Test 1 0 0 0 0 2 3 3 3

Test 2 0 0 0 0 2 2 3 3

Test 3 0 0 0 0 2 2 2 3


Table 4. Results for Bluestar® Forensic Magnum (BM)

Bluestar Magnum

Blood dilutions

Pure 1/10 1/20 1/100 1/200 1/500 1/1000 1/2000

Chemical volumes

(µL)

20

Test 1 0 0 0 0 2 2 3 3

Test 2 0 0 0 0 2 2 3 3

Test 3 0 0 0 0 2 2 3 3

60

Test 1 0 0 0 0 2 3 3 3

Test 2 0 0 0 0 1 3 3 3

Test 3 0 0 0 1 2 3 3 3

100

Test 1 0 0 0 2 2 3 3 3

Test 2 0 0 0 2 2 3 3 3

Test 3 0 0 0 2 2 3 3 3

Table 5. Results for Lumiscene Ultra (LU)

Lumiscene Ultra

Blood dilutions

Pure 1/10 1/20 1/100 1/200 1/500 1/1000 1/2000

Chemical volumes

(µL)

20

Test 1 0 0 0 2 2 2 3 3

Test 2 0 0 0 1 2 3 3 3

Test 3 0 0 0 2 2 3 3 3

60

Test 1 0 0 0 2 2 3 3 3

Test 2 0 0 0 2 3 3 3 3

Test 3 0 0 1 2 3 3 3 3

100

Test 1 0 0 0 3 3 3 3 3

Test 2 0 0 0 3 3 3 3 3

Test 3 0 0 0 3 3 3 3 3

As demonstrated in tables 2 and 3, Bluestar® Forensic produced better results according to the quality of the DNA profile at the 1:100 dilution than did Lumiscene Ultra. Results were quite similar for the remainder of the dilutions. Bluestar® Forensic did not affect the ability to obtain the known donor’s DNA profile at 1:10, 1:20 and 1:100 dilutions of blood. Similar results were obtained with Bluestar® Forensic Magnum with a slight variation at the 1:100 dilutions.

One characteristic of the Lumiscene Ultra chemical is that it is sold only for reconstruction. That is why complete profiles were given only with the 1:10 dilutions. Good results were obtained with a majority of complete profiles for the 1:20 dilutions. Beginning with the 1:100 dilutions, the results showed a strong DNA alteration.


Results at D30

Negative controls did not reveal any positive results and positive controls revealed positive results

for swabs from undiluted blood to a 1:500 dilution. The results for Lumiscene (L), Bluestar® Forensic (BS) Bluestar® Forensic Magnum (BM) and Lumiscene Ultra (LU) are presented in Table 6.

Table 6. Results at D30.

Bluestar Bluestar Magnum Lumiscene Lumiscene Ultra

20 60 100 20 60 100 20 60 100 20 60 100

Pure

Test 1 0 0 0 0 0 0 0 0 0 0 0 0

Test 2 0 0 0 0 0 0 0 0 0 0 0 0

Test 3 0 0 0 0 0 0 0 0 0 0 0 0

1/10

Test 1 0 0 0 0 0 0 0 0 0 0 0 2

Test 2 0 0 0 0 0 0 0 0 0 0 0 0

Test 3 0 0 0 0 0 0 0 0 0 0 1 0

1/20

Test 1 0 0 0 0 0 0 0 0 0 0 0 0

Test 2 0 0 0 0 0 0 0 0 0 0 0 0

Test 3 0 0 0 0 0 0 0 0 0 0 0 0

1/100

Test 1 2 2 1 1 0 2 2 1 2 2 2 2

Test 2 2 0 0 1 1 2 2 0 0 2 2 2

Test 3 2 1 2 2 2 2 2 0 2 2 0 2

1/200

Test 1 2 2 2 2 2 3 2 2 2 2 2 3

Test 2 2 2 2 2 3 3 2 2 2 2 2 3

Test 3 2 2 2 2 3 2 2 2 2 2 2 2

For Lumiscene, from undiluted blood to the 1:20 dilutions, DNA profiles qualities were the same. However, from 1:100 dilutions, light damage. Indeed, there were more scores of 2 than of 0 to the 1:200 dilutions. Scores of 3 appeared at the same dilutions than at D0.

The results for Bluestar® Forensic, as seen in experiments at D0, it is a satisfactory chemical to obtain a DNA profile at 1:10 and 1:20 dilutions. Nevertheless from the 1:100 to the 1:2000 dilutions, results were worse with a majority of scores of 2 for the 1:100 dilutions and a score of 3 for all tests from the 1:500 dilutions. The same trend can be noticed with Bluestar® Forensic Magnum with incomplete profiles obtained from the 1:200 dilutions.

Overall, profiles showed the same quality for Lumiscene Ultra at D0 and D30 with better results at D30 from the 1:100 dilutions with a majority of scores of 2 to the 1:200 dilutions. Human Blood Identification Tests


Human Blood Testing

Results at D0

Each test was analysed based the on the scoring system as presented in table 7.

Table 7. Scoring system

+++ Positive: very good eye naked line

++ Positive: good eye naked line

+ Positive: eye naked line

+- Indeterminable

- Negative

Hb tests

Negatives controls did not reveal any positive results. Positive controls revealed positive results for

undiluted blood and for the following dilutions: 1:10, 1:20, and 1:100 and weaker for 1:500 and 1:5000. The results are presented in table 8.

Table 8. Results for Hb test

HB tests

Dilutions

Pure 1/10 1/20 1/100 1/500 1/2000 1/5000

BS

Test 1 ++ +++ +++ - - - -

Test 2 ++ +++ + - - -

Test 3 ++ +++ - - - -

BM

Test 1 ++ +++ +++ + - - -

Test 2 ++ +++ + - - -

Test 3 ++ +++ +- - - -

L

Test 1 ++ +++ +++ ++ +- - -

Test 2 ++ +++ ++ +- - -

Test 3 ++ +++ + +- - -

Test 1 ++ +++ ++ +- - - -

LU Test 2 ++ +++ +- - - -

Test 3 ++ +++ +- - - -

As noted in table 8 positive results were obtained with undiluted and diluted blood (1:10 and 1:20). Results for the 1:100 dilution could be acceptable but they were better with Lumiscene. It was observed that for undiluted blood, results were somewhat worse than for the 1/10 dilution. This could be due to the “Hook Effect” which is a false negative result with certain immunoassays due to very high concentration of a particular reagent or blood.


RSID

Negative controls did not reveal any positive results. Positive controls revealed positive results for

undiluted blood and for the 1:10 dilutions as presented in table 9.

Table 9. Results for RSID

RSID

Dilutions

Pure 1/10 1/20 1/100 1/500 1/2000 1/5000

BS

Test 1 +++ + - - - - -

Test 2 +++ + - - - -

Test 3 +++ + - - - -

BM

Test 1 +++ ++ - - - - -

Test 2 +++ ++ - - - -

Test 3 +++ ++ - - - -

L

Test 1 +++ + - - - - -

Test 2 +++ + - - - -

Test 3 +++ + - - - -

Test 1 +++ + - - - - -

LU Test 2 +++ + - - - -

Test 3 +++ + - - - -

Good results were obtained with RSID for undiluted blood and for the 1:10 dilutions as shown in

table 9. The difference between these tests and Hb tests may be due to the sought element (hemoglobin for Hb tests and glycophorin A for RSID tests). Detection of glycophorin A seems to be more specific. This can explain why it could be more difficult to obtain a good result with a diluted blood than with haemoglobin detection.

A unique test with the 1:20 blood dilutions was performed for each chemical. Indeed, only the 1:10 and the 1:100 were tested in the first phase. The results for the 1:100 dilutions were significantly lower or absent. That is why it became interesting to test the 1:20 blood dilutions.

Discussion

Lumiscene provided better results for the 1:100 dilutions than the other chemicals. Moreover, most

of the results obtained for D0 and D30 show that the quality of genetic profiles are more similar with Lumiscene than with Bluestar®. Genetics profiles are the same for light dilutions for all chemicals.

Additional results for D60 will be obtained soon. Nevertheless, variations according to scores assigned to each profile were noted for D0 and D30. The effect of time was observed. The only variable parameter was the time of contact between blood and chemicals. However, chemicals did not seem to have an effect on time for undiluted blood. Hence, this trend that was observed during one month indicated that the quantity of those products has an effect on the quality of the genetic profiles of diluted blood. This may be due to the period during which chemicals were in contact with blood and/or the quantity of chemicals placed on each swab.


In this experiment, the exact number of leukocytes was unknown. It was not possible to count precisely the number of cells with a specific counter. Blood pipetting was done to allow a homogeneous distribution of cells for each dilution, hence the reason that three samples were tested for each dilution and for each volume of chemicals added.

Only 20 µL of blood dilutions were used on each swab. This parameter may explain some poor results concerning the quality of genetic profiles. We suggest that a larger quantity of blood would provide better genetic profiles.

It is important to underline the fact that no surfaces were tested in this experiment. Bloodstains and patterns are influenced by surface texture and composition upon which they were deposited or come into contact. Indeed, this can have an effect on the absorption, the size and shape of the bloodstains and may also influence the quality of samples. Conclusions

Results obtained for D0 and D30 seem indicate that Lumiscene is slightly better than Bluestar® Forensic especially at the 1:100 dilutions at the 30 day period. Lumiscene Ultra was tested with the understanding that it is used for reconstruction and not for DNA analysis. Accordingly, poorer results were obtained with a period of one month during which chemicals were in contact with blood. This result appears to be linked with the quantity of chemicals put on swab and the time of contact.

At D0, the quantity of chemicals did not significantly affect the quality of the profile. The tests also revealed that an increase in time and chemical volume more greatly affected the ability to obtain a DNA profile. This trend will be analyzed and perhaps certified with D60 results. With all results, an analysis will be done on 924 data with a statistical study. We hope to publish a scientific article in the near future with all the results and more details will be presented.

With regard to human blood detection, without results for D60, RSID provided worse results than the Hb test despite its specificity. This trend has to be confirmed with experiments at D60 which will be performed in the near future.

References

1. Gnyaneshwari Patel, Andy Hopwood. An evaluation of luminol formulations and their effect on DNA

profiling. Int. J. Legal Med. 2013; 127: 723˗729

2. BaseClear B.V., Einsteinweg 5, 2333 CC Leiden, The Netherlands. Forensic DNA analysis (STR-testing)

of human blood treated with Lumiscene. BASECLEAR. 22 August 2012. 52613 report

3. Juliana Piva de Almeida, Nadine Glesse, Cristina Bonorino. Effect of presumptive tests reagents on human

blood confirmatory tests and DNA analysis using real time polymerase chain reaction. Forensic Science

International. 2011; 206: 58-61

4. Filippo Barni, Simon W. Lewis, Andrea Berti, Gordon M. Miskelly, Giampietro Lago. Forensic application

of the luminol reaction as a presumptive test for latent blood detection. Talanta. 2007. 72: 896-913

5. Cathy J. Jakovich. STR Analysis Following Latent Blood Detection by Luminol, Fluorescein, and Bluestar.

Journal of Forensic Identification. 2007. 57(2)193-198

6. Rapid Stain Identification of Human Blood (RSID) Technical Information and Protocol Sheet, European

Distributor: Galantos Genetics, GmbH, 55128, Mainz, Germany

7. Hemoglobin (Hb) Test Technical Information and Protocol Sheet, Veda Lab, Alencon, France


The 2014 IABPA Annual Training Conference to be held in Portland, Maine September 30-October 3, 2014

The Conference is being organized by Herb Leighton, Brandi Caron and Alison Gingras of the Maine State Police.

The Conference venue will be: The Westin Portland Harborview Hotel 157 High Street Portland, Maine 04101

Visit the IAPBA website, www.iabpa.org for further information and registration.

The Fifth European IABPA Conference to be held in Rome, Italy May 12-15, 2015.

The Conference is being organized by the Arma dei Carabinieri - Forensic Science Department. IABPA 2015 will be held at the Forensic Science Department of the Arma dei Carabinieri and will consist of 3 days of lectures and caseworks.

The Conference Venue will be: Arma dei Carabinieri - Forensic Science Department HQ Viale di Tor di Quinto, 119 00191, Rome (IT)

Please visit the Website https://5theuropeaniabpaconference.com/ for a thorough overview of the Conference Venue, Registration, Transport Information and best sites to visit during your stay in the Eternal City! You may send an e-mail to [email protected] for any additional info you may need. You may also directly call the Forensic Science Department (Dr. Andrea Berti) at: +39 06 809 803 32. We’re looking forward to see you all in Rome for the Conference. On behalf of the Organizing Conference Committee we send you our best regards!

Dott. Andrea Berti

http://www.iabpa.org/

https://5theuropeaniabpaconference.com/



Organizational Notices

Moving Soon?

All changes of mailing address need to be supplied to our Secretary Norman Reeves. Each quarter

Norman forwards completed address labels for those who are members. Do not send change of address information to the Journal Editor. E-mail your new address to Norman Reeves at:

[email protected]

Norman Reeves

I.A.B.P.A.

12139 E. Makohoh Trail

Tucson, Arizona 85749-8179

Fax: 520-760-5590

Membership Applications / Request for Promotion

Applications for membership as well as for promotion are available on the IABPA website:

IABPA Website: http://www.iabpa.org

The fees for application of membership and yearly dues are $40.00 US each. If you have not received a dues invoice for 2014 please contact Norman Reeves at [email protected]. Also, apparently, non US credit cards are charging a fee above and beyond the $ 40.00 membership/application fee. Your credit card is charged only $40.00 US by the IABPA. Any additional fees are imposed by the credit card companies.

IABPA now accepts the following credit cards:

Discover MasterCard

American Express Visa

If you had had a change of address, please contact Norman.


http://www.iabpa.org/



Training Opportunities

October 13-17, 2014

Advanced Bloodstain Pattern Analysis Course

(English) Blutspureninstitut

Obergasse 20 61250 Usingen

Germany

Instructor: Dr. Silke Brodbeck, MD

Tel: +49-170-84 84248

Fax: +49-6081-14879

November 17-21, 2014

Advanced Bloodstain Pattern Analysis Course Loci Forensics B.V.

Flierveld 59 2151 LE Nieuw-Vennep

The Netherlands

Instructors: Martin Eversdijk and Rene Gelderman

Fax: +31(0)20-8907749

E-mail: [email protected]

November 17-21, 2014

Advanced Bloodstain Pattern Analysis Workshop Northeast Forensic Training Center Northampton Community College

Bethlehem, Pennsylvania

Contact: Andy Kehm, Program Director Tel: 484-201-1054


December 1-5, 2014

Basic Bloodstain Pattern Analysis Course (German)

Blutspureninstitut Obergasse 20

61250 Usingen Germany


Tel: +49-170-84 84248

Fax: +49-6081-14879




December 8-12, 2014

Bloodstain Pattern Analysis Workshop

Miami-Dade Public Safety Training Institute Doral, Florida

Instructor: Toby Wolson, M.S., F-ABC

Miami-Dade Police Department

Crime Laboratory Bureau

Forensic Biology Section

9105 N.W. 25th

Street

Doral, Florida

33172-1500

Voice: 305-471-3014

Fax: 305-471-3478


December 8-12, 2014

Visualization of Latent Bloodstain Course

Loci Forensics B.V. Flierveld 59

2151 LE Nieuw-Vennep The Netherlands

Instructors: Martin Eversdijk and Rene Gelderman

Fax: +31(0)20-8907749


February 9-13, 2015

Basic Bloodstain Pattern Analysis Course Keiser University Tampa, Florida

Instructors: Stuart H. James and Anna Cox

Further Information contact:

Anna Cox

Tel: 813-732-4001


Stuart H. James

Tel: 954-651-2865


Instructors: Stuart H. James

Anna Cox

Contact: Anna Cox

Tel: 813-732-4001







March 9-13, 2015

Bloodstain Pattern Analysis Workshop Miami-Dade Public Safety Training Institute

Doral, Florida





9105 N.W. 25th

Street

Doral, Florida

33172-1500

Voice: 305-471-3014

Fax: 305-471-3478


April 20-24, 2015

Basic Bloodstain Pattern Analysis Course

(English) Blutspureninstitut


Germany


Tel: +49-170-84 84248

Fax: +49-6081-14879

May 4-8, 2015

Basic Bloodstain Pattern Analysis Course (German)




Tel: +49-170-84 84248

Fax: +49-6081-14879



September 14-18, 2015

Advanced Bloodstain Pattern Analysis Course (German)




Tel: +49-170-84 84248

Fax: +49-6081-14879

October 19-23, 2015

Advanced Pattern Analysis Course (English)




Tel: +49-170-84 84248

Fax: +49-6081-14879

December 7-11, 2015

Basic Bloodstain Pattern Analysis Course

(German) Blutspureninstitut


Germany


Tel: +49-170-84 84248

Fax: +49-6081-14879


December 7-11, 2015

Bloodstain Pattern Analysis Workshop Miami-Dade Public Safety Training Institute

Doral, Florida





9105 N.W. 25th

Street

Doral, Florida

33172-1500

Voice: 305-471-3014

Fax: 305-471-3478


Articles and training announcements for the December 2014 issue of the Journal of Bloodstain Pattern Analysis must be received before November 15

th, 2014



Editor’s Corner

This issue features an interesting research article entitled the Effect of Four Latent Blood

Visualization Products on DNA authored by Céline Nicloux and Jessica Bressler. I thank them for

their contribution to the Journal. I invited the speakers at our upcoming IABPA Training Conference

in Portland, Maine to consider submit a paper of their topic for peer review and consideration for

publication in future issues. The December issue will feature the abstracts of papers and workshops

presented at the conference.

The National Institute of Standards and Technology (NIST) has announced their selection of

members for the Organization of Scientific Area Committees (OSAC). This is an initiative by NIST

to strengthen forensic science in the United States. Bloodstain Pattern Analysis (BPA) is part of the

Physics/Pattern Committee which also includes Firearms and Toolmarks, Footwear and Tire Tread,

Friction Ridge and Questioned Documents. Toby L. Wolson was selected as the Chair of the OSAC

Bloodstain Pattern Subcommittee. Also selected for this Subcommittee was Paul E. Kish. David

Baldwin was selected as a member for the Physics and Pattern Committee that will oversee the

Bloodstain Pattern Subcommittee. I have worked with these individuals as a member of SWGSTAIN

and on behalf of the IABPA membership I congratulate them on their new positions. I am confident

that they will represent our discipline well into the future.

Stuart H. James Editor [email protected]



Publication Committee

Associate Editors

Barton P. Epstein Paul E. Kish Daniel Mabel

Jeremy Morris Jon J. Nordby

Joe Slemko Celestina Rossi

Jeffrey Scozzafava T. Paulette Sutton

Past Editors of the IABPA News/Journal of Bloodstain Pattern

Analysis

Anita Y. Wonder 1984-1985 Norman Reeves 1984-1989 David Rimer 1990-1996 Toby L. Wolson 1997-2000 Paul E. Kish 2001-2003 Stuart H. James 2004-present

Past Presidents of the IABPA

V. Thomas Bevel 1983-1984 Charles Edel 1985-1987 Warren R. Darby 1988 Rod D. Englert 1989-1990 Edward Podworny 1991-1992 Tom J. Griffin 1993-1994 Toby L. Wolson, M.S. 1995-1996 Daniel V. Christman 1997-1998 Phyllis T. Rollan 1999-2000 Daniel Rahn 2001-2002 Bill Basso 2002-2006 LeeAnn Singley 2007-2008 Iris Dalley 2009-2010 Todd A. Thorne 2011-2012

The Journal of Bloodstain Pattern Analysis published quarterly in March, June, September, and December. 2014. The International Association of Bloodstain Pattern Analysts. All rights are reserved. Reproduction in whole or in part without written permission of the Editor and Author(s) is prohibited.