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BIOTEK CORP. The Sample Preparation Experts T 905-227-8848 E [email protected] ISO 13485:2003, ISO 9001:2008, ISO 15189:2012 Registered Rapid and convenient method to detect microorganisms in soil samples Process any soil type including clay, topsoil, loam, sandy or any high humic acid content samples such as sediments, manure and compost Remove organic substances using the OSR Solution Remove all humic acid from DNA samples Fast and easy processing using a rapid spin-column format Isolate high quality total DNA from a variety of microorganisms including Gram-positive and Gram- negative bacteria, yeast, mold, other fungi and actinomycetes etc. SOIL DNA ISOLATION PLUS KIT Norgen’s Soil DNA Isolation Plus Kit provides a convenient and rapid method for the detection of microorganisms from soil samples. All types of soil samples can be processed with this kit, including common soil samples and difficult soil samples with high humic acid content such as compost and manure. The kit removes all traces of humic acid and PCR inhibitors using the provided the OSR (Organic Substance Removal) Solution. A simple and rapid spin column procedure is then used to further purify the DNA. Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR applications, as all humic acid substances and PCR inhibitors are removed during the isolation. ISOLATE TOTAL DNA FROM ALL MICROORGANISMS Process all types of soil, including difficult soil samples with high humic acid content Warburgstr. 45 • 20354 Hamburg, Germany Fon + 49 (0) 40 45 067 0 • Fax + 49 (0) 40 450 490 [email protected][email protected] www.dianova.de Beratung und Vertrieb in Deutschland Gesellschaft für biochemische, immunologische und mikrobiologische Diagnostik mbH Warburgstr. 45 • 20354 Hamburg • Germany Fon + 49 (0) 40 45 067 0 • Fax + 49 (0) 40 450 67 490 [email protected][email protected] www.dianova.de NORGEN BIOTEK CORP. The Sample Preparation Experts Request Sample Now!

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NORGENBIOTEK CORP.The Sample Preparation Experts

T 1-866-NORGENBT 905-227-8848

F 905-227-1061E [email protected]

www.norgenbiotek.comISO 13485:2003, ISO 9001:2008,ISO 15189:2012 Registered

Rapid and convenient method to detect microorganisms in soil samples

Process any soil type including clay, topsoil, loam, sandy or any high humic acid content samples such as sediments, manure and compost

Remove organic substances using the OSR Solution

Remove all humic acid from DNA samples

Fast and easy processing using a rapid spin-column format

Isolate high quality total DNA from a variety of microorganisms including Gram-positive and Gram-negative bacteria, yeast, mold, other fungi and actinomycetes etc.

SOIL DNAISOLATIONPLUS KIT

Norgen’s Soil DNA Isolation Plus Kit provides a convenient and rapid method for the detection of microorganisms from soil samples. All types of soil samples can be processed with this kit, including common soil samples and difficult soil samples with high humic acid content such as compost and manure. The kit removes all traces of humic acid and PCR inhibitors using the provided the OSR (Organic Substance Removal) Solution. A simple and rapid spin column procedure is then used to further purify the DNA. Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR applications, as all humic acid substances and PCR inhibitors are removed during the isolation.

ISOLATE TOTAL DNA FROM ALL MICROORGANISMS

Process all types of soil, including difficult soil samples with high humic acid content

Warburgstr. 45 • 20354 Hamburg, Germany

Fon + 49 (0) 40 45 067 0 • Fax + 49 (0) 40 450 490

[email protected][email protected]

www.dianova.de

Beratung und Vertrieb in Deutschland

Gesellschaft für biochemische, immunologische und mikrobiologische Diagnostik mbH

Warburgstr. 45 • 20354 Hamburg • Germany

Fon + 49 (0) 40 45 067 0 • Fax + 49 (0) 40 450 67 490

[email protected][email protected]

www.dianova.de

NORGENBIOTEK CORP.The Sample Preparation Experts

T 1-866-NORGENBT 905-227-8848

F 905-227-1061E [email protected]

www.norgenbiotek.comISO 13485:2003, ISO 9001:2008,ISO 15189:2012 Registered

Rapid and convenient method to detect microorganisms in soil samples

Process any soil type including clay, topsoil, loam, sandy or any high humic acid content samples such as sediments, manure and compost

Remove organic substances using the OSR Solution

Remove all humic acid from DNA samples

Fast and easy processing using a rapid spin-column format

Isolate high quality total DNA from a variety of microorganisms including Gram-positive and Gram-negative bacteria, yeast, mold, other fungi and actinomycetes etc.

SOIL DNAISOLATIONPLUS KIT

Norgen’s Soil DNA Isolation Plus Kit provides a convenient and rapid method for the detection of microorganisms from soil samples. All types of soil samples can be processed with this kit, including common soil samples and difficult soil samples with high humic acid content such as compost and manure. The kit removes all traces of humic acid and PCR inhibitors using the provided the OSR (Organic Substance Removal) Solution. A simple and rapid spin column procedure is then used to further purify the DNA. Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR applications, as all humic acid substances and PCR inhibitors are removed during the isolation.

ISOLATE TOTAL DNA FROM ALL MICROORGANISMS

Process all types of soil, including difficult soil samples with high humic acid content

Request

Sample

Now!

Beratung und Vertrieb in DeutschlandWarburgstr. 45 • 20354 Hamburg • Germany

Fon + 49 (0) 40 45 067 0 • Fax + 49 (0) 40 450 490

[email protected][email protected]

www.dianova.de

NORGENBIOTEK CORP.The Sample Preparation Experts

T 1-866-NORGENBT 905-227-8848

F 905-227-1061E [email protected]

www.norgenbiotek.comISO 13485:2003, ISO 9001:2008,ISO 15189:2012 Registered

Visit our website: www.norgenbiotek.com to view our complete line of reagents and innovative sample preparation kits.

Description Prep Size Cat. #

Soil DNA Isolation Plus Kit 50 preps 64000

Ordering Information

v1.0

Figure 1. Comparison of DNA yield from top soil and clay samples.

Comparison of DNA YieldClay Top Soil

M Norgen Competitor M Norgen Competitor M

NorgenCompetitor MNTC

NorgenCompetitor MNTC

16s rRNA Detection (TaqMan)

Figure 3. High quality DNA confirmed by real-time PCR.

Clay Top Soil

0

5

10

15

20

25

30

Top Soil Clay

DN

A C

once

ntra

tion

(ng/

µL)

Norgen

Competitor M

Figure 2. Comparison of DNA Concentration isolated from top soil and clay samples.

DNA Concentration (ng/µL)

NORGENBIOTEK CORP.The Sample Preparation Experts

T 1-866-NORGENBT 905-227-8848

F 905-227-1061E [email protected]

www.norgenbiotek.comISO 13485:2003, ISO 9001:2008,ISO 15189:2012 Registered

Rapid and convenient method to detect microorganisms in soil samples

Process any soil type including clay, topsoil, loam, sandy or any high humic acid content samples such as sediments, manure and compost

Remove organic substances using the OSR Solution

Remove all humic acid from DNA samples

Fast and easy processing using a rapid spin-column format

Isolate high quality total DNA from a variety of microorganisms including Gram-positive and Gram-negative bacteria, yeast, mold, other fungi and actinomycetes etc.

SOIL DNAISOLATIONPLUS KIT

Norgen’s Soil DNA Isolation Plus Kit provides a convenient and rapid method for the detection of microorganisms from soil samples. All types of soil samples can be processed with this kit, including common soil samples and difficult soil samples with high humic acid content such as compost and manure. The kit removes all traces of humic acid and PCR inhibitors using the provided the OSR (Organic Substance Removal) Solution. A simple and rapid spin column procedure is then used to further purify the DNA. Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR applications, as all humic acid substances and PCR inhibitors are removed during the isolation.

ISOLATE TOTAL DNA FROM ALL MICROORGANISMS

Process all types of soil, including difficult soil samples with high humic acid content

Beratung und Vertrieb in Deutschland

Gesellschaft für biochemische, immunologische und mikrobiologische Diagnostik mbH

Warburgstr. 45 • 20354 Hamburg • Germany

Fon + 49 (0) 40 45 067 0 • Fax + 49 (0) 40 450 67 490

[email protected][email protected]

www.dianova.de

Soil DNA Isolation Kit Plus Order # Price*

50 Prep NGB-64000 € 248,00

100 Prep NGB-64060 € 448,00

Sample (4 Prep) NGB-64040 Request now!

* Prices excl. VAT., incl. Free Shipping in Germany, Ordering from other Countries please inquire.

Ordering Information (Germany, Austria, Switzerland)

diaN

OR_

Soil+

-JA 1

2-20

16V1

EN