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GMO presence Fusarium detection Toxin genes identification Corn + Soy bean Fusarium graminearum Fusarium culmorum Fusarium proliferatum Fusarium verticiloides TRI 7 DON TRI 13 DON TRI 7 NIV PQF5 - fumonisin 8 samples of commercial feed samples Wheat specimen spontaneously and naturally infected - collected in Romania Fusarium detection Toxin genes identification Collected from 2010 harvest 7 wheat samples, collected from three Romanian counties
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Szeged - Timisoara axis for the safe food and feed
Acronym: SZETISA 1
HURO / 0901/147/2.2.2
Szeged University
Banat University of Agricultural Sciences and Veterinary Medicine, Timisoara
January, 26th - 27th, 2012
Determination of microelements as affected by microbial toxins in wheat
2 wheat cultivars artificially infected with 2 Fusarium strains of low and high activities, respectively, in Szeged (Cereal Research Institute).
Wheat specimen spontaneously and naturally infected - collected in Romania
The analyzed material
Artificially infected wheat cultivars – moment 0 – Fusarium strain identification
the samples were negatives
The Fusarium attack was evaluated
in different organs:
parts1 grains2 chaf3 culm4 flag leaf
GMO presence
Fusarium detection
Toxin genes identification
Corn + Soy bean
Fusarium graminearumFusarium culmorumFusarium proliferatumFusarium verticiloides
TRI 7 DONTRI 13 DONTRI 7 NIVPQF5 - fumonisin
8 samples of commercial feed samples
Wheat specimen spontaneously and naturally infected - collected in Romania
Fusarium detectionToxin genes identification
Collected from 2010 harvest
7 wheat samples, collected from three Romanian counties
WHEAT SAMPLES
18 wheat samples, collected from two Romanian counties
Fusarium detectionToxin genes identificationToxin quantification
Flag leaf
Culm
Ear Chaf
Grain Fusarium quantification
The analyzed material
DETERMINATION OF MICROELEMENTS AS AFFECTED BY MICROBIAL TOXINS IN MAIZE.
2 corn cultivars - genetically modified (Bt) and conventional from different locations
5 maize samples from last year’s harvest 4 conventional varieties YG – genetically modified maize
GMO presence
Fusarium detection
Toxin genes identification
Maize samples from this year harvestgenetically modified maize – 14 from different farms
GMO presence
Fusarium detection
Toxin genes identification
Toxin quantification
SIGMA
SATELIT
Two month treatment
The plants were grown in the green house
ALFALFA metal treatment
Untreated - control
Pb 50 Cd 1 Cu 100 Fe 100
100 3 250 250
250 10 500 500
500 20
Untreated - control
Pb 50 Cd 1 Cu 5 Fe 5100 3 10 10250 10 50 50
Dried materials
INCREASE OF INFRASTRUCTURE
COOLING CENTRIFUGE - SIGMA
Multi-Sample Micro-Volume UV-Vis Spectrophotometer
The tender was organizedThe contract was signedThe equipment was installed
The tender was organizedThe contract was signedThe equipment was installed
BIOANALYZER
The tender was organized
Ioja-Boldura Oana-Maria, Popescu Sorina
A molecular approach for screening of toxigenic Fusarium graminearum in feedstuff
Bulletin of University of Agricultural Sciences And Veterinary Medicine, Volume 68 (1-2), pag. 422, Cluj-Napoca 2011, ISSN 1843-5262
COMUNICATION ACTIVITIES
THANK YOU FOR YOUR ATTENTIONS!