ORAL AND MAXILLOFACIAL PATHOLOGY OOOO

e224 Abstracts February 2014

Denture stomatitis (DS) is a frequent lesion that affects themucosa covered by a denture, especially in elderly patients. Theetiology is multifactorial, but is strongly associated with Candidaalbicans. We quantified different cells in DS lesions clinicallyclassified as simple or diffuse inflamed mucosa (DS1) or hyper-plastic surface (DS2) under a maxillary complete denture(papillary hyperplasia). The molecules CD3, CD4, CD8, CD20,CD68, and mast cell tryptase were labeled by immunohisto-chemistry in 32 DS cases and 6 healthy human palatal mucosasamples (control) obtained from the files of the Anatomical Pa-thology Laboratory of FOB/USP. DS1 lesions demonstrate acellular infiltrate similar to that of controls. However, in the DS2group, CD4 and CD8 lymphocytes, mast cells, and B cells weremore prevalent in connective tissue. These changes may indicatean association between aspects of immune response and differentclinical presentations of DS.

PE-468 - INDUCTION OF APOPTOSIS IN DENTURESTOMATITIS BY CANDIDA ALBICANS. ANA REGINACASAROTO, RAFAELA ALVES DA SILVA ALAVARCE,SAMIRA SALMERON, MARIA FÁTIMA GUARIZOKLINGBEIL, MONICA BEATRIZ MATHOR, MARIALÚCIA RUBO DE REZENDE, VANESSA SOARES LARA.BAURU SCHOOL OF DENTISTRY, UNIVERSITY OF SÃOPAULO.

The presence of Candida albicans in adherent biofilmunder prostheses is related to denture stomatitis (DS). Thisstudy evaluated the ability of C. albicans to induce apoptoticevents in human epithelium cells in vitro. Study Design: Cellsobtained from palatal tissue were challenged by direct andindirect contact with viable C. albicans (0.01/1, 0.025/1, and0.1/1 yeast/keratinocyte) at 3, 6, and 10 hours. Apoptotic cellswere determined by fluorescent nuclear staining, expressed as apercentage of the total number of cells counted, and evaluatedusing contrast and/or Mann-Whitney tests (p < 0.05). Results:In the direct assay, the ratio of 0.025/1 caused significantapoptosis at the 3-hour assessment compared with other times.The increase of the fungal concentration resulted in significantcell apoptosis compared to unchallenged epithelium. The samewas observed for the indirect assay. Conclusion: These resultssuggested that C. albicans induces early epithelial apoptosis inthe DS.

PE-469 - MDM2 AND SUMO-1 EXPRESSION INACTINIC CHEILITIS AND LIP CANCER. ADRIANA DAMOTA DELGADO, MÔNICA GHISLAINE OLIVEIRAALVES, TABATA DE MELO TERA, IVAN BALDUCCI,YASMIN RODARTE CARVALHO, ANA SUELI RODRIGUESCAVALCANTE, JANETE DIAS ALMEIDA. UNIVERSIDADEESTADUAL PAULISTAeUNESP.

This study compared the expression of MDM2 and SUMO-1proteins between actinic cheilitis and squamous cell carcinoma(SCC) of the lip. Methods: The sample consisted of lower lipmucosa specimens obtained from patients with a clinical andhistopathological diagnosis of actinic cheilitis (n ¼ 26) and SCC(n ¼ 25) as well as specimens of semimucosa (n ¼ 15) withoutclinical alterations. The tissue samples were stained with hema-toxylin-eosin and anti-MDM2 and anti-SUMO-1 antibodies.Data were analyzed by Kruskal-Wallis and Dunn’s tests (5%).Results: The median expression of MDM2 and SUMO-1 wassimilar in cases of actinic cheilitis and SCC of the lip, butdiffered significantly from that observed for semimucosa

specimens. Conclusion: We observed an overexpression ofMDM2 and SUMO-1 in cases of actinic cheilitis and lip SCC, sowe believe that it could be related to the regulatory mechanismsof apoptosis.

PE-470 - CONNECTION BETWEEN MONONUCLEARCELL INFILTRATE AND THE EXPRESSION OF KI-67,COLLAGEN TYPE IV, AND LAMININ IN RADICULARCYSTS. RENATA VERAS CARVALHO MOURÃO, ANAPAULA NEGREIROS NUNES ALVES, ALCEU MACHADODE SOUSA, DENNYS RAMON DE MELO FERNANDESALMEIDA, ANA LARIYSSA FERREIRA GOMES PORTO,MÁRIO ROGÉRIO LIMA MOTA, ERASMO BERNARDOMARINHO. UNIVERSIDADE FEDERAL DO CEARÁ.

Radicular cyst (RC) and dentigerous cyst (DC) have differentetiopathogenesis. This study evaluated the relationship betweeninflammatory infiltrate, expression of proliferative immuno-markers, proteins of basement membrane (BM), and extracellularmatrix in RC. The expression of Ki-67, collagen type IV (C-IV),and laminin were examined in highly inflamed RC (HIRC)(n¼17), slightly inflamed RC (SIRC) (n¼9), and DC (n¼9). Theimmunoexpression of Ki-67 was higher in SIRC than in HIRCand DC. The immunoexpression of C-IV in the BM of the SIRCwas lower than in HIRC and DC. The anti-laminin expression inBM of HIRC and DC was negative, and in SIRC it was focal. Itwas concluded that severity of inflammatory content in the RCwall appeared to modify the expression of proliferation factors inthe BM, but not in the extracellular matrix.

PE-471 - SURVIVAL ANALYSIS AND FOLLOW-UPOF MALIGNANT ODONTOGENIC TUMORS. THAISSOUSA DA SILVA, DANIELA OTERO PEREIRA COSTA,PAULO ANTÔNIO FARIA, DANIELLE RESENDECAMISASCA, FERNANDO LUIZ DIAS, SIMONELOURENÇO, VANESSA SOARES LARA. INSTITUTONACIONAL DO CÂNCER (INCA) - RJ, BRASIL.

This study presents the clinicopathologic features, survival,and follow-up of a series of malignant odontogenic tumors.Methods: Fourteen cases of malignant odontogenic tumors werereviewed. Sociodemographic and clinicopathologic data wererecorded. Survival was analyzed using the Kaplan-Meier method.Results: The most common tumor was ameloblastic carcinoma(50.0%), followed by malignant odontogenic tumors not other-wise classified (21.43%). The average age was 46.2 years old, themale to female ratio was 1.6:1, and the maxilla was the site mostoften affected (64.28%). Surgical resection was the treatmentemployed in all cases. Mean overall survival (OS) and disease-free interval were 73.88 months and 54.93 months, respectively.Survival analysis identified maxilla (p ¼ 0.05) and diagnosis (p ¼0.006) as being associated with the worst OS. Conclusions: Theseneoplasms present an aggressive and sometimes lethal course,especially tumors in the maxilla, which was the most meaningfulparameter in survival analysis.

PE-472 - SYNDECAN-1 EXPRESSION IN SOLID AME-LOBLASTOMA AND CALCIFYING CYSTIC ODONTO-GENIC TUMOR. SABRINA NOGUEIRA DE MORAES,FABIO RAMOA PIRES, LUCIANA ARMADA DIAS,REBECA SOUZA AZEVEDO. FACULDADE DE ODONTO-LOGIA DE PIRACICABAeUNICAMP.

Ameloblastoma (AME) and calcifying cystic odontogenictumor (CCOT) are benign odontogenic tumors having some

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Volume 117, Number 2 Abstracts e225

microscopic similarities but different clinical behavior. Synde-can-1 (SDC-1) is a transmembrane heparin sulfate proteoglycanthat is usually downregulated in aggressive tumors and shiftedfrom epithelial to stromal cells in invasive tumors. This studycomparatively evaluated the immunohistochemical expressionof SDC-1 in AME and CCOT. Study Design: SDC-1 immu-nohistochemical expression was analyzed in 17 cases of solidAME and 6 cases of CCOT. Results: SDC-1 epithelial expres-sion was identified in all cases of solid AME and CCOT,whereas SDC-1 stromal expression was identified in 40% of thecases of solid AME and 17% of the cases of CCOT. Conclusion:SDC-1 epithelial expression seems to have a role in both solidAME and CCOT development. SDC-1 stromal expression maybe involved in the invasiveness of solid AME. Financial sup-port: FAPERJ.

PE-473 - EFFECTS OF ZOLENDRONATE IN CHRONICPERIAPICAL LESIONS: AN EXPERIMENTAL STUDY INRATS. TALITA RIBEIRO TENÓRIO DE FRANÇA, JUREMAFREIRE LISBOA DE CASTRO, MARCO ANTÔNIO GOMESFRAZÃO, FLÁVIA MARIA DE MORAES RAMOS-PEREZ,DANYEL ELIAS DA CRUZ PEREZ. UFPE.

This study evaluated the histopathological features of chronicperiapical lesions and adjacent bone tissue in animals treated withzolendronate. Study Design: Forty male rats were used. Initially,the pulps of the first mandibular molars were exposed. Subse-quently, the animals were divided into 8 groups: GI and GII -Periapical lesion induction (PLI) and weekly intraperitonealadministration (WIPA) of saline solution for 4 and 8 weeks,respectively; GIII and GIVePLI and WIPA of zolendronate (0.15mg/kg/week) for 4 and 8 weeks; GV and GVIeWIPA of salinesolution for 4 and 8 weeks and subsequent PLI; GVII and GVIII -WIPA of zolendronate for 4 and 8 weeks and subsequent PLI.Results: Periapical lesions were characterized by chronicinflammation, with no difference between groups. No osteonec-rosis was observed in any specimen. Conclusion: Chronic peri-apical lesions apparently are not a risk factor for osteonecrosisinduced by bisphosphonates.

PE-474 - IMMUNOEXPRESSION OF HIF-1A ANDMEASUREMENT OF DENSITY IN LOWER LIP SQUA-MOUS CELL CARCINOMA IN METASTATIC ANDNON-METASTATIC CASES: A RELATIONSHIP WITHPROGNOSIS PARAMETERS. CLARISSA FAVERODEMEDA, ANA RAFAELA LUZ DE AQUINO, CYNTIAHELENA PEREIRA DE CARVALHO, CASSIANOFRANCISCO WEEGE NONAKA, LÉLIA BATISTA DESOUZA, LEÃO PEREIRA PINTO. FEDERAL UNIVERSITYOF RIO GRANDE DO NORTE.

This study evaluated the immunoexpression of HIF-1a andcorrelated it with intratumoral and peritumoral lymphatic densityin metastatic and non-metastatic lower lip squamous cell carci-noma (LLSCC). Study Design: This study evaluated the expres-sion pattern and the percentage of cells immunostained forHIF-1a in 50 cases of LLSCC; 25 had regional nodal metastasisand 25 did not. Lymphatic microvessel density was determined.Results: HIF-1a exhibited predominantly diffuse cytoplasmicstaining in tumors. The analysis of intratumoral and peritumorallymphatic density showed no significant association with clini-copathological parameters and immunoexpression of HIF-1a(p > 0.05). A positive correlation was noted between intratumoraland peritumoral lymphatic density (p ¼ 0.004). The percentage of

nuclear positivity for HIF-1a was significantly higher in caseswithout invasion of peritumoral lymphatics (p ¼ 0.040).Conclusion: This study suggests that diffuse cytoplasmicexpression of HIF-1a can contribute to a better prognosis forLLSCC. It does not directly influence the increased lymphaticmicrovessel density.

PE-475 - IMPACT OF BETHANECOL ON XEROSTOMIA-INDUCED RADIATION AND ITS EFFECT ON QUALITYOF LIFE IN HEAD AND NECK CANCER PATIENTS.DANIEL CAMPANHÃ, JULIANA ROCHA VERRONE,BRUNA DE CASSIA SABINO, JOSÉ DIVALDO PRADO,GRAZIELLA CHAGAS JAGUAR. A. C. CAMARGO CAN-CER CENTER.

This study assessed whether the prophylactic use of betha-nechol during radiotherapy (RT) reduces xerostomia in head andneck cancer patients and what effects it has on quality of life(QoL). Methods: We conducted a prospective randomized dou-ble-blind study with 97 patients allocated into two groups:bethanechol (n ¼ 48) and placebo (n ¼ 49). Xerostomia severitywas assessed weekly from baseline to 3 months after completionof treatment. Results: All patients completed the University ofWashington Quality of Life Questionnaire before RT, duringtreatment, and 3 months after RT. QoL significantly worsenedwith increased time in both groups. However, 3 months after RT,xerostomia severity was significantly higher in the placebo group(p < 0.001) with a significant impact on saliva secretion (p ¼0.009) and mastication (p ¼ 0.041) domain scores. Conclusion:The present study supports the concept that using bethanecolduring RT increases saliva secretion, significantly decreasesxerostomia, and improves QoL.

PE-476 - THE IMPACT OF DENTAL CARE BEFOREAND AFTER HEAD AND NECK RADIOTHERAPY.ALEXANDRE FRASCINO, KARIN SÁ FERNANDES,CAROLINA MUSSI, MARINA GALLOTTINI. FACULDADEDE ODONTOLOGIA DA UNIVERSIDADE DE SÃO PAULO.

This study compared the impact of dental care before andafter radiotherapy. Study Design: Records of all irradiatedpatients seen at CAPE-FOUSP were reviewed. Informationcollected concerned demographic profile, tumor location, dentalconditions, and procedures performed. Patients were allocatedinto two groups: group 1, patients who had dental care beforeradiotherapy, and group 2, patients who had dental care afterradiotherapy. Results: The sample population consisted of 76%men, 85% were white, and the average age was 52 years. Squa-mous cell carcinoma affected 69% of patients, mostly in thetongue (22.37%). Twenty patients (13%) were allocated intogroup 1 and 139 (87%) into group 2. A comparison of cariesprevalence showed statistically significant differences (p ¼ 0.01).Similar results were found for periodontitis, gingivitis, andxerostomia (p ¼ 0.02, 0.04, and 0.09; respectively). Group 2patients needed 29% more dental procedures than group 1 pa-tients. Conclusion: Dental care before radiotherapy decreases theprevalence of oral complications.

PE-477 - IMPACT OF ORAL HEALTH ON QUALITY OFLIFE FOR PATIENTS DIAGNOSED WITH CANCER.ANDREZA BARKOKEBAS SANTOS DE FARIA, DÉBORAHFONSECA, IGOR HENRIQUE MORAIS, ELIZABETHMARQUES, LUIZ ALCINO GUEIROS, ALESSANDRACARVALHO, JAIR CARNEIRO LEÃO. UFPE.