The world leader in serving science

June, 9th - Sorrento

Symposium – Innovating Solutions For Today and Tomorrow

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•>55,000 employees

•50 countries

•>$800 million spent on R&D

•5 premier brands

The World Leader in Serving Science

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Thermo Fisher Scientific - Animal Health

• Ready to use kits for qPCR or ELISA

• Reagents to build up your own

workflow from sampling and

extraction/purification up to detection

• Instruments and services

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Our Origins

Access to a broad and strong selection of innovative diagnostic tools for production

animals

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• Most of our veterinary diagnostic

solutions benefit from world wide

recognitions:

• Licenses

• Validations

• Registrations & Import permits

• OIE registration

• Our Manufacturing sites are ISO 9001

certified

• Where applicable, production in USDA

licensed facilities

Quality. Applied.

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Global Animal Health Sites

France, Lyon: dedicated manufacturing and R&D

site for molecular biology solutions

NL, Lelystad : Dedicated manufacturing

site for ELISA solutions

USA, Austin: Dedicated to R&D

and manufacturing site (in particular

USDA licensed solutions)

Switzerland, Schlieren: Dedicated to R&D

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• For more than 20 years, we have been

developing innovative diagnostic tools

• These products are now

available in more than 80 countries

worldwide

We leverage our global network and regional

expertise to help meet the unique product &

support requirements of our customers

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Our Diagnostic Solutions For Veterinary Labs

• PRRS

• Swine influenza

• Coronaviruses

• ASF, CSF

• PCV2

Swine

• Trichinella

• Salmonella

• Hepatitis E

• Mycobacterium avium

• Toxoplasma

Harvest panel

• BVD

• Bovine trichomonosis

• Abortive infections

• Paratuberculosis

• Bovine tuberculosis

Ruminant

Workflow

• Sample Collection

• Sample Preparation : Applied BiosystemsTM MagMAXTM reagents technology & Thermo FisherTM KingFisherTM instruments

• qPCR instruments : 7500 Platforms, Applied BiosystemsTM QuantStudioTM 5

• Master Mixes and IPCs

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Porcine Pathogen Products

*Artif icial digestion

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Ruminant Pathogen Products

* Other test formats include lateral flow immunochromatographic, agglutination, and western blot

assays.

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Other Animals Pathogens Products

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Collect Sample

Prepare Sample

Add Reagents

Incubate Wash Add

Reagents Analyze Results

• TypiFix™

• GenoTube™

• Precellys™ Evolution

Homogenizer™

• SL 40 Centrifuge

Series

• Applied Biosystems™

PrioCHECK™ ELISA

Kits

• Finn™ Pipettes

• Pipettes

• Tips

• Wellwash™

Microplate Washer

• Wellwash Versa

Microplate Washer

• PrioCHECK ELISA

Kits

• Finn Pipettes

• Pipettes

• Tips

• Thermo Scientific™

Multiskan™ FC

Microplate Photometer

• Multiskan GO

Microplate

Spectrophotometer

• Thermo Scientific

SkanIt™ Software

ELISA Workflow

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Collect Sample Prepare Sample Perform nucleic

acid isolation Prepare qPCR Perform qPCR Analyze Results

• TypiFix

• GenoTube

• Applied Biosystems ™

VetMAX™ Xeno IPC

DNA/RNA

• Applied Biosystems™

MagMAX™ Core

Nucleic Acid Purification

kit

• Thermo Scientific ™

KingFisher™ Duo

Prime

• KingFisher Flex 96

• KingFisher Plastics

• VetMAX Diagnostic

Kits

• VetMAX reagents

• VetMAX Xeno IPC

Assay

• Applied Biosystems™ 7500 Fast real-time

PCR instrument

• Applied Biosystems™ QuantStudio™ 3 & 5

real-time PCR system

• Plastic Ware

• Optical Film

qPCR Workflow

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Let’s work today on molecular diagnostic workflow

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Discover how our newest

advances and technologies

help you and your laboratory

The world leader in serving science

June, 9th – Sorrento Andrea Biserni (EMEA Animal Health FAS Manager)

Meeting today’s diagnostic needs & innovate your workflow for tomorrow’s challenges

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Sampling DNA/RNA

Extraction and Purification

Real-time PCR

Detection

Molecular Workflow for Vet Diagnostics

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TypiFix: A Stable Sample For a Reliable Diagnosis and/or Genotyping

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Room Temperature Stable Nucleic Acids

• TypiFix samples stored for 8 years at room temperature prior to DNA extraction.

• Competitors samples were stored for 3 months at room temperature prior to DNA extraction. Electrophoretic

separation on a 0.8% agarose gel

-> In case of TypiFix no relevant DNA degradation is detected

TypiFix

Competition

8 years

3 months

TypiFix vs. competition

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Sampling DNA/RNA

Extraction and Purification

Real-time PCR

Detection

Molecular Workflow for Vet Diagnostics

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MagMAX CORE - Concept

• Core Solution

• Application-specific modules

• Coupling ‘CORE’ with one or

more ‘Modules’

Offers labs:

• Flexibility

• Familiarity

• Less Waste

• New Applications

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MagMAX CORE – Key Characteristics

• Pathogen RNA/DNA purification for vet diagnostics + host gDNA

purification for genotyping application

• Only two washes steps needed

• Novel formulation, compatible with sequencing applications

• All reagents are ready to use (all washes pre-mixed and no

additional alcohol needed)

• Room Temperature storage

• Optimized magnetic beads provide best performance and

automation with all throughtput capabilities (low to high

throughput)

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ACCELERATE, OPTIMIZE and EVOLVE your daily routine

ACCELERATE:

• Fast instrument processing times: quicker diagnostic results; • Ready to use washes: Less preparation time and no need for extra

alcohols orderings and addition; • Greater sensitivity with simplified workflows

OPTIMIZE:

• Helps to reduced plastics, limiting waste and costs;

• Room temperature reagents: improved product handling & saves on

refrigerated storage space and costs;

• Same protocol for multiple KingFisher magnetic particle processors: same

workflows regardless of throughput

EVOLVE:

• A ‘CORE’ chemical formulation designed to be a universal solution that

address 14 typical Vet sample types;

• Flexibility of having modules to couple with the ‘CORE’ chemistry, to easily

address all new applications without needing to introduce a total new

chemistry

• New formulation and workflow that allows for downstream sequencing

applications

MagMax CORE Value

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MagMAX CORE - Sample Matrices

MagMAX CORE Nucleic Acid Purification Kit has been optimized for

use with more animal matrices than any other kit on the market

• Whole blood

• Serum

• Feces

• Oral fluid

• Animal, fecal, and environmental swabs

• Tissue/organs

• Milk

• Semen

• Ear notches

• Ear punches

• Environmental samples

• Plasma

• Hair follicles

• Biomed Diagnostics InPouch™ TF (Tritrichomonas foetus) culture

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MagMAX CORE Nucleic Acid Purification Kit has been

validated on multiple KingFisher magnetic particle processors

• KingFisher Flex

• KingFisher Duo Prime

• KingFisher mL

• MagMAX Express-96 / KF-96

MagMAX CORE - Instrumentation

27 min run time (script with heated elution)

37 min run time (script without heated elution)

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MagMAX CORE kit format

MagMAX CORE Nucleic Acid Purification Kit 500 reactions

MagMAX CORE Nucleic Acid Purification Kit 100 reactions

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Sampling DNA/RNA

Extraction and Purification

Real-time PCR

Detection

Molecular workflow for Vet diagnostics

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What makes your real-time PCR diagnosis efificient and reliable?

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Animal Health Master Mixes Portfolio

Thermo Fisher Scientific has more than 300 master mix skus In Animal Health portfolio we selected 3

Master mixes optimized for Vet needs

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Do you have all required safety check point in your real-time PCR analysis ?

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• Xeno (RNA or DNA) IPC- serves as an internal positive control for the purification process and/or

monitors for the presence of PCR inhibitors

• 10,000 copies/uL

• RNA or DNA controls

• Xeno IPC Assays (VIC™ or LIZ™) - is a primer/probe mix that detects the Xeno IPC. The

resultant Xeno data is used to determine the validity of diagnostic test results.

• VIC and LIZ labeled dye options

• 25X concentration

A safety tool in your Real Time PCR analysis

VetMAX™ Xeno™ IPC System:

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With VetMAX Xeno IPCs and Assays

Signal with the Xeno control

Real negative!

No signal in your PCR Target assay

No Signal with the Xeno control assay No signal in your PCR Target assay

False negative !

(PCR inhibition

or poor extraction)

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VetMAX Xeno IPC and Assay

Make your real-time PCR diagnosis reliable

• Easily integrated into any qPCR

workflow

• Help provide confidence that qPCR test

results are accurate and actionable

• Help prevent the risk of false negatives

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Introducing the expanded line of real-time PCR

and digital PCR systems

QuantStudio Systems

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QuantStudio for qPCR Only qPCR

and

dPCR

Digital

PCR

System

Color Combinations 4 5 or 21 5 21 21 2

Reactions/Run < 96 < 384 < 384 < 384 < 12,288 20,000

Block Options

96 0.1mL 96 0.1mL

96 0.1mL 96

0.2mL 384

96 0.1mL

96 0.2mL 384,

TaqMan Array

96 0.1mL

96 0.2mL

384,

TaqMan Array

OpenArray™

1 chip 96 0.2mL

96 0.2mL 384

Run Time (minutes)

< 30 < 30 > 30 > 30 > 30 30 sec.

reads

QuantStudio 3 & 5 Systems and the qPCR Family

3 5 6 7 12 3D

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QuantStudio 3 & 5 Real-Time PCR System

• Different color combinations, allowing a broad range multiplexing.

QuantStudio 3: 4 color combination

QuantStudio 5: 5-21 color combination

• Intuitive and easy use for all levels of experience with touch screen

interactivity

• Connects to the Thermo Fisher cloud to access and analyze your data

anytime, from anywhere with an internet connection.

• Precise temperature control with VeriFlex blocks

• Ability to lock a protocol template and Built-in security, auditing, and

electronic signature (SAE) package

• Factory calibrated, ready for quick installation and use

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Dye Combinations Using OptiFlex System

QuantStudio 3

96-well

QuantStudio 5

384-well

QuantStudio 5

96-well

White LED for long lasting performance

Pre-calibrated dyes, ready to work right out of box

Peak

channel Color Filter wavelenth (nm) Pre-calibrated

dyes Example custom dyes

Excitation Emission

x1-m1 Blue 470±15 520±15 FAM™ and SYBR™ Green SYTO™ 9

x2-m2 Green 520±10 558±12 VIC™ HEX™ , TET™ and JOE™

x3-m3 Yellow 550±10 587±10 ABY™ , NED™ and TAMRA™ Cy®3

x4-m4 Orange 580±10 623±14 JUN™ and ROX™ Texas Red™

x5-m5 Red 640±10 682±14

Cy®5 and MUSTANG

PURPLE LIZ™

x6-m6 Deep-red 662±10 711±12 None Cy®5.5

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Ultimate Thermal Control

VeriFlex Blocks technology

• QuantStudio 3: 3 programmable zones

• QuantStudio 5: 6 programmable zones Temperature flexibility

• Independently controlled peltier blocks

• Precise temperature control

• Enhanced PCR functionality

96-well 6 zone

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Software Interface

• Interactive touch screen

• Monitor progress during run

• Access run files multiple ways

• Creating runs and access data anywhere

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Monitor Progress During the Run

Live Amplification Curves Thermal Protocol Status Time Remaining

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USB

Setup: Stand-alone, Desktop, or Online

Desktop software

PC or Mac®

Connect with a web browser

Wi-Fi

LAN

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QuantStudio 3 & 5 Connects Researchers Worldwide

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Connectivity Simplified

Collect: uploading and storing of data files

Maintain control of your experiment data with upload to your cloud account from

cloud-enabled instruments or from your desktop computer.

Monitor: Remote monitoring of real-time instruments

Stay connected to cloud-enabled instruments and bench-top devices anytime,

anywhere, and from any mobile device to see availability, run progress, and more.

Analyze: Intuitive web-based apps

Perform primary and secondary analysis across all your data and projects with a

robust toolkit of fast and powerful analysis software.

Store: Secure, scalable data storage

Easily save and synchronize your files to your secure, personal cloud account.

Share: Discretionary access to project folders and data sets

Use secure connections to invite colleagues and collaborators to review results and

perform new analyses.

Powered by Thermo Fisher Cloud

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It’s your life’s work—we’ll help you protect it

Security and privacy are our highest priority. We understand that the confidentiality of data and

protocols are critical, both to your research and to the publication process. To ensure high

levels of protection and security against compromise to your data, we’ve built Thermo Fisher

Cloud on the world-class Amazon Web Services™ (AWS) cloud-computing platform. With

AWS, all of your data is stored in highly secure AWS data centers managed by compliance

programs and audit safeguards.

• Data encryption

• Network firewalls

• Scheduled audits

• Data backup and recovery

• Data sharing

Security Matters

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We offer a full portfolio of products for Vet Diagnostics

• Ready to use kits for real-time PCR, sequencing or

ELISA

• Other reagents to build up your own workflow from

sampling and extraction/purification up to detection;

• instruments and services for the animal health

industry.

Designed to provide tools to diagnose and control disease

and improve general health and welfare of livestock.

47 The world leader in serving science

Questions?

48 The world leader in serving science

For Veterinary Use Only. For In Vitro Use Only. Regulatory requirements vary by country; products may not be available in your geographic area. © 2017 Thermo Fisher Scientif ic Inc. All rights reserved. All trademarks are the

property of Thermo Fisher Scientif ic and its subsidiaries unless otherw ise specif ied..

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