Syllabus of MSc (Micro) OU

8/13/2019 Syllabus of MSc (Micro) OU

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DEPARTMENT OF MICROBIOLOGY, OSMANIA UNIVERSITY

M.Sc. (Previous MICROBIOLOGY (Se!es"er S#s"e!

S#$$%&us, Sc'e!e o I)s"ruc"io) %)* E+%!i)%"io)

Se!es"er I(15 Weeks)

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Paper Sub.Code Subject/Paper Title Instruction Ea! "a

#o. $rs/Wk Ti!e $rs "arks

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T$E%&'

I " 11 *eneral "icrobiolo+, 1

II " 1 0irolo+, 1

III " 1 io!etr, and Co!puters 1

I0 " 1 ioce!istr, 1

P&2CTIC23S

I " 151 *eneral "icrobiolo+, a -

II " 15 0irolo+, b -

III " 15 io!etr, and Co!puters c -I0 " 15 ioce!istr, d -

" 144 Se!inar - -

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Total . 6

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Se!es"er II(15 Weeks)

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Paper Sub.Code Subject/Paper Title Instruction Ea! "a

#o. $rs/Wk Ti!e $rs "arks

------------------------------------------------------------------------------------------------------------

T$E%&'

I " 1 "icrobial P,siolo+, 1

II " I!!unolo+, 1

III " 2nti!icrobial 2+ents and Ce!oterap, 1

I0 " En7,!olo+, and ioce!ical Tecni8ues 1

P&2CTIC23SI " 51 "icrobial P,siolo+, 9(a) 1

II " 5 I!!unolo+, 9(b) 1

III " 5 2nti!icrobial 2+ents and Ce!oterap, 9(c) 1

I0 " 5 En7,!olo+, and ioce!ical Tecni8ues 9(d) 1

" 44 Se!inar - -

------------------------------------------- Total . 6

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#ote: (a) Te ea!ination includes bot " 151 and " 51 s,llabi

(b) Te ea!ination includes bot " 15 and " 5 s,llabi

(c) Te ea!ination includes bot " 15 and " 5 s,llabi

(d) Te ea!ination includes bot " 15 and " 5 s,llabi


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M.Sc. (Previous I Se!es"er

P%er I MB -- Ge)er%$ Micro&io$o/# (0 U)i"s + -1 2rs 3 4 2rs "e%c'i)/

U)i" I

$istor, o; "icrobiolo+,. "icroscop,. Structure o; !icrobial cells:Spontaneous +eneration and +er! teor, o; disease< Contributions o; 2nton, =an

3eeu>enock< 3ouis Pasteur< &obert ?oc< Ed>ard @enner< Wino+radsk,< eijerinkorkin+ o; bri+t ;ield !icroscope< ;luorescent !icroscope< pase contrast

!icroscope< electron !icroscope. 2pplication and i!portance o; abo=e !icroscopes.

"easure!ent o; !icroscopic objects. Prokar,otic cell< Eukar,otic cell< %r+ani7ationand ;unction o; cellular or+anells

Cell >all o; bacteria< cell !e!branes< ;la+ella< pili< capsule structure< ce!ical structure

o; peptido+l,can< protoplasts< speroplasts< !icroso!es and riboso!al s< #uclear!aterial/nucleus.

U)i" II

"etods o; sterili7ation: P,sical !etods Br, eat< !oist eat< radiation !etods o; !edical !icrobiolo+, b, @a>it7< !elnick and 2delber+

acterial and ",cotic in;ections o; !an. Ed. Bubos and $irst 3ipincott

Principles o; "icrobiolo+, and I!!unolo+, b, Ba=is< Bulbecco < Eison< *insber+ andWood.

"icrobiolo+, b, Pelc7ar ".@.< &ied< &B and Can< ECS.

Structure and &eproduction o; 2l+ae< 0ol. I F II b, Aritsc< A.E.

Introduction to 2l+ae b, "orris< I.Products and Properties o; 2l+ae b, Gi7ac.

Ares >ater al+ae o; te Hnited States b, S!it< *".

Introductor, ",colo+,< b, 2leopolus< [email protected] in Aun+i b, In+old< CT

"icrobial P,siolo+, b, "oater !osaic =irus

U)i" II

Structure and replication o; bacteriopa+es: 3,tic ds linear B#2 =iruses (T< T)

3,so+enic ds linear B#2 =irus (3a!bda) ss Circular B#2 =irus (J K 1) "alespeci;ic ;ila!entous ss =irus (A1 and "1)

U)i" III&eco!bination in pa+e< !ultiplicit, reacti=ation and penot,pic !iin+.

Structure and replication o; ani!al =iruses (2deno=irus (e+. T,pe )

U)i" IV

Structure and r eplication o; ",o=iruses (e+. In;luen7a) Po =irus (e+. 0accinia)

$epatitis =irus.

E;;ect o; ani!al =irus in;ection on ost cell 0iral inter;erence and inter;eron Tu!or

=iruses (B#2 and )

I Se!es"er P%er II MB -16 Viro$o/# (Pr%c"ic%$s

Isolation o; pa+e ;ro! soil.Culti=ation and preser=ation o; pa+es

Luantitation o; pa+es

*ro>t pa+es o; pa+e and burst si7e

Isolation o; pla8ue t,pe and ost ran+e !utantsPa+e induction

3,so+en,-Transduction

Propa+ation o; ani!al =iruses in e++ allantoic< a!niotic and C2"Propa+ation o; =iruses in tissue culture

Pla8ue titration and neutrali7ation

Luantitation o; =irus b, $2< Pock titration< pla8ue titrationIdenti;ication o; =irus $2I

Be!onstration o; c,topatolo+ical can+es

S,!pto!atic obser=ations o; plant =iral in;ections

Cloroplast a++lutination o; plant =iruses


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Reco!!e)*e* Boo5s

*eneral 0irolo+, b, 3uria and Barnel

0irolo+, and I!!unolo+, b, @okliTet book o; 0irolo+, b, &odes and 0an &o,en

Plant 0irolo+, b, S!it

*enetics o; bacteria and teir =iruses b, W. $a,es"olecular iolo+, o; te +ene b, Watson< &oberts< Stait7 and Weiner

2 laborat+or, +uide in =irolo+, b, Cjarles $. 3unnin+a!

asic lab procedures in dia+nostic =irolo+, b, "art, Cristensen


P%er III MB -7 Bio!e"r# %)* Co!u"ers

(0 u)i"s + -1 'rs 3 4 'rs "e%c'i)/

U)i" I

Introduction to statistics and bio!etr,

Population< sa!ples and sa!plin+ procedures< =ariables< =ariations and ;re8uenc,

distributions< !easures o; a=era+es and dispersion< ele!ent o; probabilit,< +ausian ornor!al distribution< bino!ial distribution< poisson distribution< MtD distribution< MAD

distribution and Ci-s8uare distribution< correlation and linear re+ression >it a special

re;erence to applications in biolo+,

U)i" II

Tests o; in;erence and concepts

S!all sa!ple tests and lar+e sa!ple tests: #or!al cur=e test< MtD test< MAD test< 2#%02at is $ard>are and so;t>are< brie;

introduction on input output de=ices (Bisks< Printers< CB-&%"S and oter stora+e

!edia etc.)Introduction to disk operatin+ s,ste!s (B%S): Sa!ple co!!ands< BI&-CB-&B-BE3-

C%P'-"%0E-&E#-T'PE-EBIT (Editor) CE-B2TE and TI"E.

Introduction to Windo>s 45: Icons< Besktop< Ailes and Aolders.Si!ple operations like creation< deletion< !o=in+< cop,in+ o; Ailes or ;olders usin+

>indo>s eplorer. Searcin+ ;iles and ;olders. Creatin+ sortcuts and oter si!ple

operations.


6/28

U)i" IV

Word Processin+: %penin+< creatin+ and sa=in+ docu!ents T,pin+< na=i+atin+ andselectin+ editin+ and sortin+-ceckin+ spellin+ and +ra!!ar ;or!attin+ can+in+

appearance o; ,our pa+e i!portin+ +rapics >orkin+ >it tables >orkin+ >it land

docu!ents Printin+Electronic Spread Seet: Creatin+< openin+ and sa=in+ ;iles >orkin+ >it >orkseets

and >ork books enterin+ data and selectin+ cells editin+ >orkseets printin+orkin+ >it carts Su!!ari7e data in lists and tables 2nal,7in+data >it Pi=ot tables 2nal,7in+ sa!ple statistical data 0alidatin+ cells.

I Se!es"er P%er III MB -17 Bio!e"r# %)* Co!u"ers (Pr%c"ic%$s

Aor!ation o; ;re8uenc, distribution< +rapical presentation and calculation o; descripti=e

!easures

Aittin+ o; distribution ino!ial< Poisson and #or!al distributionCi-s8uare test< +oodness o; ;it< contin+enc, and etero+eneit,

S!all and lar+e sa!ple tests< sa!ple !eans and propertiesProble!s on correlation and re+ression

2#%02 %ne >a, and t>o >a, classi;ications

Creatin+ docu!ents usin+ >ord processorEercisin+ te ;eatures o; >ord processor

Creatin+ spread seets

Hsa+e o; spread seet to biolo+ical applications

Reco!!e)*e* Boo5s

Introduction to te teor, o; statistics b, 2leander< " "ood and Aranklin

Aunda!entsls o; io!etr, b, 3.#.ala!

Statistical !etods b, Snedecor and CocranIntroduction to co!puter and its application b, Cae C.Cien

asic Pro+ra!!in+ lan+ua+e b, ajara!an

iostatistics 2 !anual o; statistical !etods ;or use in $ealt< #utrition and

2ntropolo+, b, ?. 0is=es>ar &ao


7/28


P%er IV MB -0 Bioc'e!is"r# (0 u)i"s + -1 'rs 3 4 'rs "e%c'i)/

U)i" I

p$ and its biolo+ical rele=ance

Beter!ination o; p$< preparation o; bu;;ersConcept o; entrop,< ;ree-ener+,< ;ree ener+, can+es< i+ ener+, co!pounds.

E8uilibriu! constants< &edo potentials< iolo+ical redo s,ste!s

U)i" II

iolo+ical oidation< iolo+ical redo carriers< biolo+ical !e!branes< electron

transport< oidati=e pospor,lation and !ecanis!. acterial potos,ntesist

!easure!ent.

U)i" IV

#ature and properties o; spores: acterial endospore structure< peno!enon o;

sporulation< bioce!istr, and +enetics o; sporulation. Induction o; sporulation

peno!enon. *er!ination o; sporesTe concept o; disease: In;ectious disease< pato+enic !icrobes< properties o;

pato+enic !icrobes< In;ection< pato+enesis and =irulence. 0irulence ;actore

Capsular !aterials< bacterial toins eotoins< tooids< endotoins< enterotoins.P,siolo+, o; toin production. Etracellular en7,!es o; pato+enic bacteria.

2pplication o; toins and toic en7,!es< laborator, testin+ !etods ;or bacterial

=irulence properties


9/28

II Se!es"er P%er I MB 61- Micro&i%$ P'#sio$o/# (Pr%c"ic%$s

Preparation o; !icrobiolo+ical !edia. 2utotropic !edia< !ini!al !edia< basic !ediait7< !elnick and 2delber+

acterial and ",cotic in;ections o; !an. Ed. Bubos and $irst 3ipincott

Principles o; "icrobiolo+, and I!!unolo+, b, Ba=is< Bulbecco < Eison< *insber+ and

Wood.Tet book o; "icrobiolo+, b, 2nantanara,anan

"icrobiolo+, b, Pelc7ar ".@.< &ied< &B and Can< ECS.

"icrobial P,siolo+, b, "oatt b, "andelstu!< "c Luillon and Ba>es

acterial "etabolis! b, B>ellel,Potos,ntesis b, Be>lin and arker

3aborator, Eperi!ents in "icrobiolo+, b, *opal &edd, et al

"icrobes in 2ction b, Seole, $W and 0an-Be!ark< P@


10/28

M.Sc. (Previous II Se!es"er

P%er II MB 66 I!!u)o$o/# (0 u)i"s + -1 'rs 3 4 'rs "e%c'i)/

U)i" I

rie; istor, o; i!!unolo+,< anti+ens (t,pes o; anti+ens anti+en speci;icit,a,s.


11/28

II Se!es"er P%er II MB 616 I!!u)o$o/# (Pr%c"ic%$s

2++lutination reactions Widal< 0B&3< $2< lood +roup/t,pin+Precipitation test: &in+ interpase< sin+le radial di;;usion< %uecterlon,

Testin+ ;or dru+ sensiti=it,

Beter!ination o; "IC =alues (tube dilution !etod)Beter!ination o; penol coe;;icient (&eidel Walker coe;;icient< &WC)

Stud, ;or anti!icrobial spectru! o; anti!icrobials

Beter!ination o; cidal and static acti=it,Screenin+ ;or antibiotic producin+ !icrobes

Ce!ical assa, !etods ;or anti!icrobial dru+s

"icrobiolo+ical assa,s o; anti!icrobial dru+s

Reco!!e)*e* &oo5s

Bisin;ection< sterili7ation and preser=ation Ed. lock< SS.

"icrobial conta!aination control ;acilities Ed. &unkle< &S and Pilips< *Principles and !etods o; sterili7ation in ealt science b, Perkins @on< @.

Inibition and destruction o; te !icrobial cell Ed. $u+o

ioce!istr, o; anti!icrobial action b, Aranklin< T@ and Sno>< 3.Te "olecular basis o; antibiotic action b, *ale et al

2ntibiotics and ce!oterap, b, *errod et al

Par!aceutical !icrobiolo+o, b, $u+o &ussell

"icrobiolo+ical assa,s b, $e>itt2nti=iral dru+s b, S.?ar+er

ur+ersD !edicinal ce!istr, (all =olu!es) Ed. "an;ield E. World.

2ntibiotic interactions b, Willia!sTe control o; antibiotic resistant bacteria b, $arris and $arris


14/28

M.Sc. (Previous II Se!es"er P%er IV MB 60 E)8#!o$o/# %)* Bioc'e!ic%$

Tec')i9ues (0 u)i"s + -1 'rs 3 4 'rs "e%c'i)/

U)i" I

En7,!es no!enclature< classi;ication !etods ;or deter!ination o; en7,!e acti=it,.

Isolation and puri;ication o; en7,!es. En7,!e kinetics: E;;ect o; p$< substrateconcentration < te!perature and inibitors. Isoen7,!es. Co!petiti=e and non-

co!petiti=e inibition

U)i" II

"ecanis! o; en7,!e action 2ction o; &ibonuclease< c,!otr,psin< and tr,psin.Coen7,!e catal,sis. "ecanis! o; P2KP% and tia!ine p,ropospate en7,!es

Control o; en7,!es. &e+ulation o; en7,!e acti=it,: allosteric en7,!es and ;eed back

!ecanis!s. "etabolic co!parta!entali7ation in relation to en7,!e< en7,!es and

secondar, !etabolites

U)i" III

%ptical !etods: Ele!entar, treat!ent o; bioce!ical !etods< colouri!etr, and

spectropoto!etr,< ;luro!etr,< optical rotation< ;luorescence

Seperation !etods: Cro!ato+rapic tecni8ues paper< tin la,er< ion ecan+e< +el;iltration and a;;init, cro!ato+rap,. Bi;;usion< dial,sis< cell disruption !etodsei+t o; B#2< resol=ed on a+arose +el electroporesis

Beter!ination o; !olecular >ei+t o; protein b, P2*E

Induction o; !utations b, p,sical/ce!ical !uta+ens< screenin+ and isolation o;!utants< &eplica platin+ tecni8ue

Trans;or!ation in bacteria

Conju+ation in bacteria

Co!ple!entation tests in bacteriaProtoplast preparation and re+eneration

Reco!!e)*e* &oo5s

"olecular biolo+, b, Ba=id and Arei;elder

"icrobial +enetics b, Ba=id and Arei;elder*enetics o; bacteria and teir =iruses b, Willia! $a,es

"olecular biolo+, o; tee +ene b, Watson et al

Te ioce!istr, o; nucleic acids b, Ba=idson @#"olecular biotecnolo+, b, Pri!erose

"olecular iotecnolo+, b, ernard &. *lick and @ack @ Pasternak

B#2 "icroarra,s Ed. ". Scena

M.Sc. (Fi)%$ III Se!es"er Micro&io$o/#

P%er II MB 76 I)*us"ri%$ Micro&io$o/# (0 u)i"s + -1 'rs 3 4 'rs "e%c'i)/

U)i" I

Introduction to industrial !icrobiolo+,. Be;inition< scope< istor,< !icroor+anis!s and data !inin+ o;

se8uence data bases. B#2 se8uencin+ !etodolo+, and so;t >are< se8uence

co!parison and ali+n!ent. Concept o; par!aco+eno!ics and its applicationIP& and patentin+ Concept and o=er=ies

P%er I MB 01- Mo$ecu$%r Bio"ec')o$o/# (Pr%c"ic%$s

&estriction !appin+PC& tecni8ue de!onstration

Plas!id detection and Isolation o; Plas!id B#2

Preparation o; co!petent cells and trans;or!ation

*ene clonin+ in bacteria de!onstration


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Soutern trans;er de!onstration

Be!onstration o; &A3P/2A3P

Induction o; en7,!es 3ac operonTransposon !uta+enesis

Bial,sis and separation o; proteins b, colu!n cro!ato+rap,

Reco!!e)*e* Boo5s

"olecular biolo+, b, Ba=id and Arei;elder

"icrobial +enetics b, Ba=id and Arei;elder

*enetics o; bacteria and teir =iruses b, Willia! $a,es

"olecular biolo+, o; tee +ene b, Watson et alTe ioce!istr, o; nucleic acids b, Ba=idson @#

"olecular biotecnolo+, b, Pri!erose

"olecular iotecnolo+, b, ernard &. *lick and @ack @ Pasternak

B#2 "icroarra,s Ed. ". Scena

M.Sc. (Fi)%$ IV Se!es"er Micro&io$o/#

P%er II MB 06 Micro&i%$ Bio"ec')o$o/# (0 u)i"s + -1 'rs 3 4 'rs "e%c'i)/

U)i" I

Aer!entati=e production o; insustrial alcool< uses< ra>!aterials< !icroor+anis!sort< ;er!entation and reco=er,

Aer!entati=e production o; beer "ediu! co!ponents< !alt< !alt a djuncts< opsater. Preparation o; >ort< !asin+< >ort boilin+< !icroor+anis!< inoculu! preparationine !akin+ Aruit selection< pickin+< crusin+< sulpite additionater - $epatitis ($20)< &!k< Polio !,elitis

Betailed stud, o; =iruses trans!itted b, Goonosis &abies< @apanese encepalitis

U)i" III

Betailed stud, o; contact and seuall, trans!itted =iral diseases S!all po< $erpes

($erpes si!ple =irus)< $epatitis =iruses and teir diseases

2c8uired i!!unode;icienc, s,ndro!e (2IBS)

U)i" IV

"alaria< 2!oebiasis< $el!itic in;ections (&ound >or!s< $ookd >or!s) o; !edical !icrobiolo+, b, @a>it7 et al

"edical laborator, "anual ;or tropical countries 0ol I F II b, "onica Ceesbrou+

Tet ook o; "icrobiolo+, b, 2nantanara,anan and @a,ara! Paniker0iral and &icketsial in;ections o; "an b, $ors;all and @a!

Tet book o; 0irolo+, b, &odes and 0an &o,an

0irolo+ical Procedures b, "itcal askin+

0irolo+o, b, Wilson and Tople,

Documents

Syllabus of MSc (Micro) OU