Syllabus 2330 FALL 2014

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BENG 2330: Biomaterials

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BENG 2330 Properties of Biomaterials

Fall Semester, 2014

Instructor Dr. David Britt. Contact: Canvas email, Phone: 797-2158

Office hours: Monday 2pm-3pm or by appointment

TAs/graders Sara Gertsch, Sean Bedingfield, Jonathan Valiente (Contacts: Canvas email)

Meets Lecture: Mon, Wed 4:00-4:50, ENGR 304 (ENGR 406) Note: At least one Wed class period will be used to attend a seminar from expertsspeaking about a range of fundamental and contemporary issues in chemistry and

biochemistry related to course topics.

Lab section 1, (2330-501): Tue 3:00-4:50, ENGR 408* Lab section 2, (2330-502): Thur 3:00-4:50, ENGR 408*

(If needed, some labs may occur in lieu of lectures on M and/or W) *Some labs require use of instruments in the EL-20 laboratory

Prerequisites BIOL 1610, CHEM 1210, CHEM 1215, BENG 1890, BENG 1880.Students lacking these courses must receive permission from the COE Advisor.Pre-requisite reading materials for review (e-copies on Canvas) that studentsshould be familiar with prior to this course:

- Callister Chapter 1 (general chemistry, atoms, bonding, forces)- Stryer Chapter 1 (biochemical time-scales, size-scales, forces, water)

Overview BENG 2330 provides a hands-on overview of natural and synthetic materials withapplication in medical, bioprocessing, and bioenvironmental engineering disciplines.Structure function relationships in biological materials and methods of materialscharacterization, with a focus on laboratory technique and instrumentation are

presented. Technical writing fundamentals and elements of engineering design are

emphasized through detailed, concise lab reports and topic presentations.

Pre-class time : read text and e-material, purview lecture notes, watch pre-lecture video, take pre-class topic background e-quiz

In-class / lab time : lecture, Q&A, group problem solving, labs Post-class : topic integration quiz, data analysis & writing (labs) Pre-lab time : read the lab handout and watch any pre-lab tutorials Post-lab time : compile, analyze and share data; write lab report

Textbooks Required Reading (print and online versions available through publisher. The e-version is valid for 180 days) Biomaterials: The Intersection of Biology and

Materials Science. J.S. Temenoff and A.G. Mikos. Pearson Prentice Hall, 2008)

eText ISBN-13: 978-0-13-603783-5 Print ISBN-13: 978-0-13-009710-1http://www.coursesmart.com/givecoursesmartatry?xmlid=9780136037835&__instructor=2168685We will not cover the text sequentially as it is divided roughly in two parts:

Chapters 1 7 cover fundamentals of materials science, structure andcharacterization methods, physical and mechanical properties testing, biomaterialdegradation, fabrication and processing, bulk vs. surface properties.

Chapters 8 14 cover materials in biological environments and host response.For example, prior to discussing the phenomenon of biomaterial surface fouling by

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proteins, we will first review protein structure and function, to better understand howwe can engineering non-fouling surfaces using passive and active surface coatings.The aim is to provide a thorough understanding of materials science, with specificrelevance to biomaterials, as well as a broad background in how materials behave in

biological settings, and in particular, to distinguish properties and applications ofsynthetic materials vs. biologically derived materials.

Supplementary Reading and course pre-requisite reading : Select chapters areavailable electronically through Canvas and http://www.ncbi.nlm.nih.gov/booksSupplementary reading is provided for clarification / expansion of the primary text(it is not required, but may be beneficial). Pre-requisite reading is material that youshould know prior to BENG 2330 and is provided as necessary background.

1. Fundamentals of Materials Science and Engineering , Fifth Edition, William D.Callister, Jr, (John Wiley & Sons, Inc. 2001).

2. Our Molecular Nature: The Bodys Motors, Machines and Messages, David S.Goodsell (Springer-Verlag 1996).

3. Biochemistry, 7 th Edition, Lubert Stryer, Jeremy M. Berg, John L. Tymoczko(W.H. Freeman and Company, Ltd. 2002). Select chapters available on BB e-

reading. This text is required for CHEM 3700. Open search access to the 5th

addition is @ http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=stryer

4. Molecular Biology of the Cell (MBOC), Alberts et. al. (Garland Science) openaccess @ http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=mboc4

5. Biomaterials: An Introduction, 3 rd Ed., Joon Park, R.S. Lakes (Springer, 2007)

Grading 10% : Weekly Quizzes on Canvas (2 quizzes total per week)o Pre-class /lab reading (vocabulary) assessment quiz (5%). Available by

Sunday. Complete before cutoff time : Monday at 4:00pmunlessotherwise posted.

o Post-class /lab topic integration quiz (5%). Available Wed. after class.Complete before cutoff time : Friday at midnight unless otherwise

posted.o Quizzes cover lecture material for both Monday and Wednesday. Inaddition to quizzes some homework may be assigned.

30%: Laboratories (100 pts each). A hardcopy and e-copy are both due atstart of class on following Wednesdaylate reports will receive a zero.

o 50 pts: Lab-time :! Preparation and readinessread the handout prior to lab! (10 pts)! Attendance and attention (10 pts)! Participation and teamwork (10 pts)! Data collection in lab-book and sharing (10 pts)! Clean-up of bench tops and instruments (10 pts)

o 50 pts: Written Report: ! Topic / concept comprehension in main body and correctly

answering questions in the lab handout (10 pts)! Format: following journal article template + appendices (5 pts)! Legible, descriptive Figures and Tables (10 pts)! Spelling, grammar, and technical writing (20 pts)! Citation of primary literature (not Wikipedia) and comparison of

your results with literature (5 pts)o Deductions: Written Report , Technical writing : 1 point will be deducted

for each typographical error (spelling), incomplete/ incoherent sentence(grammar), incorrect use of a word (e.g. affect vs. effect), wrong subject

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verb agreement (e.g. the data was/were analyzed), non-parallel sentencestructure, deviation from report format, missing page numbers. Points will

be deducted for incorrect answers on lab handout questions (to be includedas an appendix at the end of the report; see lab report format pages 5-6).

o IMPORTANT: Carefully read all instructor comments on returned reports,as these will assist you in improving subsequent reports. Incomplete,hastily compiled lab reports may be returned without a grade. You are notexpected to enter this course knowing how to be an expert technicalwriteryou are expected to gradually improve your writing skills withtime and much effort. Effective communication is one of the mostimportant skills to have upon entering industry or graduate studies.

10%: Midterm 1 (In class, closed notes, closed books)

10%: Midterm 2 (In class, closed notes, closed books)

20%: Open Lab Project and Presentation (student selected topic; 10 min oral presentation graded by peers (10%).

20%: Comprehensive Final (In class, closed notes, closed books)

Letter Grades At a minimum, the University Grading Scale will be used: A 100-93%, A- to90%, B+ to 87%, B to 83%, B- to 80%, C+ to 77%, C to 73%, C- to 70%, D to 60%,F below 60%.

EthicalConduct

Students are expected to abide by the rules of conduct expected of all universitystudents. Laboratory exercises will be in teams as will some in-class problemsolving. Homework and lab reports must reflect individual effort; however, studentsare encouraged to form study groups and work as teams. This does not mean copyingwork, rather outline approaches together and work solutions individually, thencompare results. Failure to properly cite sources is plagiarism. Be certain not to cutand paste material from the Internet for your lab reports and assignments. Be certainto properly reference (cite) materials (graphs, pictures, tables, videos) used for your

presentations.

Class / Lab schedules (subject to changeupdates will be posted on Canvas)Quizzes Due Mon and Fri. Lab reports due the Wed following lab

Pre-Class / lab In Class (M and W) Lab Activities (Tue or Th)

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Temenoff Chpt 1Refresher: Callister Chpt 1(atoms, bonds)Referesher: Stryer Chpt 1(biology, size scales)Bond types:http://chemistry.about.com/video/Ionic-v-Covalent-Chemical-Bond.htm

Course overview: All coursematerials on Canvas.Classes of materials: Metals,Ceramics, Polymers, Composites;Biological vs. Synthetic materialsMaterials, matter, atomic structure,

physical attributes, & measurement

Lab 0 . Lab orientation, safety, andgood laboratory practice protocols.Lab-books, instrument logbooks,lab report format. Assignment oflab partners (2 people/group).Safety glasses required.

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Temenoff Chpt 2Supplemental: Callister Chpt 3

Interactive models of solids:http://www.chemeddl.org/resources/models360/solids.php (click on Primary MetallicStructures tab and then explore theHCP, FCC, BCC, PC structures)

Atomic to macro organization:Atomic packing factor (APF)understood through bulk density and

porosity macro-calculations. Atomicdiffusion: Ficks 1 st Law.

Lab 1 . Atomic packing factor(APF) calculations: packing of

beads and void spacedetermination. Molecular diffusionvs. porosity

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Temenoff Chpts 2-3; 6.1-6.2;6.7; 7.2.3.3Supplemental: Callister Chpt 7

Physical properties of biomaterials.Atomic defects, grains, crystallinity,thermal transitions, processinginduced transitions.

Lab 2 . Nanoparticle synthesis*, purification, characterization:Hydrodynamic radius via dynamiclight scattering (DLS)characterized in BE 1890)

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Midterm 1 REVIEW MondayMIDTERM 1 WEDNESDAY

Lab 2b . Nanoparticle drymorphology via SEM, AFM.

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Temenoff Chpt 4Supplemental: Callister Chpt 7 Mechanical properties of biomaterials. Modes of mechanicaltesting: Instron tensile test. Plastic vs.elastic deformation. Stress, strain,elastic modulus, Poissons ratio.

Lab 3a . Silicone biopolymer preparation with varied x-linkingdensities and non-foulingadditives). Instron demo: Tensilestrength; Creep; Adhesion;Compliance

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Temenoff Chpt 5Review Ficks Law of Diffusion(Chpt 2)

Biodegradation, dissolution, erosion,corrosion. Passive vs. activemechanisms: hydrolytic, enzymatic.Biomineralization.

Lab 3b . Silicone biopolymertensile characterization:mechanical properties vs. x-linking/ additives

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Temenoff Chpt 7; 1.6.2Supplemental: Callister Chpt 4

Surface vs. bulk properties. Surfacemodification and characterization.

Lab 4a . Silicone biopolymersurface patterning, incorporation of

NPS, and characterization: Contactangle analysis, light microscopy,atomic force microscopy.

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Midterm 2 REVIEW MondayMIDTERM 2 WEDNESDAY

Instructor attending nanoUtah /COMS conference in SLC

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Temenoff Chpts 7 and 14 (p444-454)Biofilm backgrounds:https://www.youtube.com/watch?v=BiY8zK8MtD0

https://www.youtube.com/watch?v=qBdYnRhdWcQ

https://www.youtube.com/watch?v=M_DWNFFgHbE

Surfaces, interfaces, adsorption, biofilms and biofouling. Surface passivation. Protein structure andadsorption kinetics.

Lab 4b . Antimicrobial /antifouling properties of native andsurface patterned / nanoparticleamended silicone biopolymers.Fluorescence microscopy /spectroscopy, SEM.

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Temenoff 1.6.2; Chpts 7-8Supplemental: MBOC, searchmembrane structureStryer: Chpt 3CMC and self assembly:http://exploringorigins.org/fattya

cids.html

Biological building blocks, self-assembly, and supramolecularstructures: vesicles, membranes,monolayers

Lab 5. Self-assembly, detergentactivity, and critical micelleconcentration (CMC) calculationusing a visible spectroscopy.Micelle size determination withdynamic light scattering.

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Temenoff Chpt 9Supplemental: Goodsell Chpt 1,Stryer Chpts 2-3Modeling amino acids:http://www.chemeddl.org/resour ces/models360/search.php?option=and&keyword=amino

Macromolecules: Synthetic vs. Natural. Bulk properties and Testing.Biological materials hierarchy,structure / function relationships:Proteins, DNA, polysaccharides

Lab 6. Protein 3-D structures andmodeling in-silico . Bring a laptopwith Internet access.

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Future directions, applications, andcontemporary issues in biologicalengineering

Open Lab for final lab project

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Open Lab for final lab project Open Lab for final lab project

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Open Lab for final lab project M,THANKSGIVING BREAK Wed

Open Lab for final lab project(Tue)

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5 Group Presentations (M, W) Final Exam review sessions

during Lab times

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FINAL EXAM : Comprehensive 2hExam (closed books, notes)

Details for Lab Reports and Final Lab Project and Presentation

Lab reports: The data from the laboratory experiments will be analyzed and summarized in concise reportsfollowing the format posted on Canvas. Students must purchase safety glasses and a laboratory notebook forrecording data and observations . Students may work in groups to analyze the data, however, the reports are to bewritten individually. A print copy is due at the start of class on the following Wednesday for both T and Th labsections. The e-copy is due at the same time . The e-copy is a backup in case your hardcopy turns up missing orthere is a dispute as to when it was submitted. The e-copy will serve as the official time stamp.

General Lab Write Up Instructions (Conference Paper Format, see examples) All lab reports will be in the formof conference abstracts: 1 page written (max of 2 pages if a more in-depth lab). Generally, a single Figure or Table isincluded with the conf. abstract text; however, we will include all Figures and Tables in Appendix 1 (see below).Refer to each Figure, Table in order in the Discussion Section.

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Specific Lab Report Format:1. Title (across top)2. Author list (Primary author: you; secondary: lab partner)3. Abstract (very concise) + Keywords below (3 to 4 descriptive terms)4. Introduction (brief, focused, some literature review)5. Materials and Methods (key techniques, methods, instruments, chemicals)6. Results and Discussion: Refer to each Figure, Table in sequence / discuss / compare and contrast data / trends

with literature (if relevant; cite the literature). Comment on any deviation from anticipated results.7. Brief Conclusions (be concise, do not refer to Figures or introduce new ideas here; no citations here)8. Reference(s) (Failure to properly cite the literature is ethical misconduct)9. Appendices:

Appendix 1: Figures and Tables with descriptive, concise captions (max of 2 per page) Appendix 2: Division of tasks between lab partners and individual contributions (writing of report is an

individual effort; data collection, analysis, presentation is a group effort). Appendix 3: Address any questions given in the lab handout Appendix 4: Scanned copies of your lab-book.

In your lab book list objectives, observations, any protocols you develop, modifications to original protocol,descriptive schematics if helpful, instrument type / model, and raw data. You do not need to paste protocols from thehandouts. Make observations and document them. Number, date, and put your initials on each page. This is a goodpractice for auditing purposes. The lab book is both data archive and evidence of work. The more polishedevidence is in the form of reports, journal articles, and conference presentations / posters / and associatedconference abstracts (hence the effort here). With regard to intellectual property a lab book documents when youfirst came up with an idea, protocol, or design. A detailed, legible, dated, and signed lab book is the foundation ofgood research. Finally, write in ink. Cross-out errors, but do not completely obscure the writing.

Quizzes: Pre-class vocabulary quiz based on assigned ready and pre-class instructor video; Post-class topic quiz.Quizzes must be completed before cutoff times on Canvas

Exams: In class, closed book / notes.

Student Open Lab Topic Presentations: Oral Presentation (maximum of 4 students per group) Students may choose any topic of interest that falls within the realm of biomaterials, medicine, or related

contemporary issues. First clear topic and supplies / instruments needed with instructor. This is an opportunity for students to investigate contemporary topics using the instruments and techniques

learned throughout the course. Oral presentation graded by the class based on clarity, knowledge of material,ability to answer questions.

Written: 1 page "conference extended abstract" type summary (less is more). Must be turned in 1-week prior tostart of oral presentations so that a book of abstracts can be compiled and posted on Canvas. Each student isto write two questions regarding their topic and include as an appendix to the abstract. The presentationshould provide direct or indirect information that provides the answers to your questions.

Oral: 12 min presentation with 3 min for questions from audience. (Next group is opening their presentation on thePC at this time). The TA will act as moderator, and a timer will be used to cut off speakers at exactly 10minutesrehearse your presentations and make sure that the slides / videos work well on the classPC/projector.

Clear your topic / demonstration with instructor before presentation deadline.

Course Outcomes and Objectives1. Understand the importance of materials properties to engineering design2. Understand natural and synthetic materials and their applications in biological engineering and biomedical

settings3. Understand relationships between composition, structure, and properties of biological materials in bulk and

at interfaces

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Withdrawal Policy and "I" Grade PolicyStudents are required to complete all courses for which they are registered by the end of the semester. In some cases,a student may be unable to complete all of the coursework because of extenuating circumstances, but not due to poorperformance or to retain financial aid. The term 'extenuating' circumstances includes: (1) incapacitating illnesswhich prevents a student from attending classes for a minimum period of two weeks, (2) a death in the immediatefamily, (3) financial responsibilities requiring a student to alter a work schedule to secure employment, (4) change inwork schedule as required by an employer, or (5) other emergencies deemed appropriate by the instructor.

Documents

Syllabus 2330 FALL 2014