Syed Lab Weekly MEA meetingSession 2. DEP experiment plans

J. James Jun

Physics HonoursUniversity of Calgary

Feb 9, 2007

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Organization of this presentation

Phase I. DEP Experiment with microsphere

Phase II.Tracking & Guiding Software

Phase III.DEP Experiment with Neuron

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DEP experiment with MicrosphereMovement control on Single Grid

GoalTo demonstrate the proof of principleTo find optimum nDEP frequency & amplitude

Procedures1. Isolate individual Microsphere2. Handle with pulling pipette3. Place onto MEA4. Apply AC signal on MEA5. Record a movie

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DEP with Microsphere Movement control on Dual Grids

GoalTo pass a microsphere between grids

Procedures1. Locate a microsphere on Grid 12. Apply nDEP until bead reaches the centre3. Turn off the nDEP4. Wait until the microsphere moves to Grid 25. Turn on the nDEP field on the Grid 2

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Tracking & Guidance Software

GoalTo track a movement of microsphereTo guide a movement of microsphere

Development PlanFast image acquisitionObject tracking w/ Fluorescent imagingFunction generator controlling software

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Tracking & Guidance SoftwareFront End

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Tracking & Guidance SoftwareBack End

Vision Tracking

Motion Coordinator

Camera

,bead

x y

Function Gen.

Electrode Selector

0#, ,CH V f

#Electrode

Image

Thread DeviceLegend:

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Summary of my work on December & January

Questions?

Phase I - DEP w/ Microsphere

Movement control on Single Grid

Movement control on Dual Grids

Phase II -Tracking & Guiding SW

Phase III - DEP w/ neuron