Embed Size (px)
Citation preview
Supporting Information
for Proteomics
DOI 10.1002/pmic.200401285
San-Yuan Huang, Jyh-Hung Lin, Yu-Huey Chen, Chin-kai Chuang, En-Chung Lin,
Mu-Chiou Huang, Hsiao-Fang Sunny Sun and Wen-Chuan Lee
A reference map and identification of porcine testis proteins using 2-DE and MS
ª 2005 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim www.proteomics-journal.de
Supp
lem
enta
ry T
able
1. P
orci
ne te
stis
pro
tein
spot
s ide
ntifi
ed b
y M
ALD
I-TO
F pe
ptid
e m
ass f
inge
rprin
ting.
Spot
no.#
Prot
ein
nam
eSp
ecie
sG
eneB
ank
Acc
essi
on N
o.
No.
of
pept
ide
mat
ched
/
No.
of
pept
ide
sear
ched
Sequ
ence
Cov
erag
e
of m
atch
ed
pept
ides
Mas
s
tole
ranc
e,
ppm
Theo
retic
al
Mr/
pI
Expe
rimen
tal
Mr/
pI
T001
vinc
ulin
Sus s
crof
agi
|179
7961
317
/37
2125
011
7374
/5.8
996
891/
5.99
T002
vinc
ulin
Sus s
crof
agi
|179
7961
318
/73
2010
011
7374
/5.8
995
699/
5.84
T003
vinc
ulin
Sus s
crof
agi
|179
7961
327
/57
3210
011
7374
/5.8
996
651/
5.78
T005
170
kDa
gluc
ose
regu
late
d pr
otei
n G
RP1
70 p
recu
rsor
Mus
mus
culu
sgi
|764
3979
14/4
414
100
1113
40/5
.12
1330
13/5
.06
T006
170
kDa
gluc
ose
regu
late
d pr
otei
n G
RP1
70 p
recu
rsor
Mus
mus
culu
sgi
|764
3979
15/3
617
100
1113
40/5
.12
1313
81/5
.02
T007
150
kDa
oxyg
en-r
egul
ated
pro
tein
pre
curs
or (O
rp15
0)C
rice
tulu
s gri
seus
gi|1
0720
182
18/6
120
100
1114
30/5
.09
1380
32/5
.00
T008
oxyg
en re
gula
ted
prot
ein
(150
kD)
Rattu
s nor
vegi
cus
gi|2
0302
024
11/2
312
200
1114
48/5
.11
1374
65/4
.99
T009
oxyg
en re
gula
ted
prot
ein
(150
kD)
Rattu
s nor
vegi
cus
gi|2
0302
024
1123
1320
011
1448
/5.1
113
6338
/4.9
6
T010
oxyg
en re
gula
ted
prot
ein
(150
kD)
Rattu
s nor
vegi
cus
gi|2
0302
024
14/3
813
200
1114
48/5
.11
1357
78/4
.95
T011
alan
yl tr
na sy
nthe
tase
Mes
ocri
cetu
s aur
atus
gi|2
6522
952
15/5
722
200
1074
46/5
.34
9617
4/5.
31
T012
alan
yl tr
na sy
nthe
tase
Mes
ocri
cetu
s aur
atus
gi|2
6522
952
11/3
316
150
1074
46/5
.34
9617
4/5.
26
T013
heat
shoc
k 70
kDa
prot
ein
4 is
ofor
m a
Hom
o sa
pien
sgi
|383
2703
922
/51
3210
095
127/
5.11
9617
4/4.
99
T014
heat
shoc
k 70
kDa
prot
ein
4 is
ofor
m a
Hom
o sa
pien
sgi
|383
2703
921
/46
3210
095
127/
5.11
9596
3/4.
96
T015
DN
A p
olym
eras
eTh
erm
opla
sma
volc
aniu
m
gi|1
4325
054
13/4
919
100
9206
1/5.
6989
552/
6.17
T017
prog
ram
med
cel
l dea
th 6
inte
ract
ing
prot
ein
Hom
o sa
pien
sgi
|220
2753
818
/47
2620
096
590/
6.13
8722
7/6.
06
T018
osm
otic
stre
ss p
rote
in 9
4H
omo
sapi
ens
gi|3
1077
164
19/4
327
200
9545
3/5.
6388
965/
5.55
T019
osm
otic
stre
ss p
rote
in 9
4H
omo
sapi
ens
gi|3
1077
164
15/4
221
200
9545
3/5.
6389
161/
5.51
T020
endo
plas
min
pre
curs
orSu
s scr
ofa
gi|1
7865
698
16/5
420
100
9269
8/4.
7589
749/
5.27
T021
1. V
CP
prot
ein
Hom
o sa
pien
sgi
|482
5709
810
/20
2515
071
534/
4.94
9014
3/5.
11
2. tr
ansi
tiona
l end
opla
smic
retic
ulum
ATP
ase
Sus s
crof
agi
|214
4498
10/2
020
150
8993
1/5.
1390
143/
5.11
T022
gp96
/GR
P94
Sus s
crof
agi
|223
9253
24/7
337
100
9274
2/4.
7589
946/
5.02
T023
gp96
/GR
P94
Sus s
crof
agi
|223
9253
16/4
222
100
9274
2/4.
7590
714/
4.78
T024
gp96
/GR
P94
Sus s
crof
agi
|223
9253
31/7
641
100
9274
2/4.
7590
341/
4.70
T025
gp96
/GR
P94
Sus s
crof
agi
|223
9253
31/6
442
200
9274
2/4.
7590
341/
4.67
T026
ubiq
uitin
spec
ific
prot
ease
5 (i
sope
ptid
ase
T)M
us m
uscu
lus
gi|7
3056
1919
/85
2620
096
685/
4.89
8799
6/4.
82
T027
euka
ryot
ic tr
ansl
atio
n el
onga
tion
fact
or 2
Hom
o sa
pien
sgi
|450
3483
16/5
919
200
9624
6/6.
4187
036/
6.96
T028
euka
ryot
ic tr
ansl
atio
n el
onga
tion
fact
or 2
Hom
o sa
pien
sgi
|450
3483
14/2
717
100
9624
6/6.
4187
036/
6.78
T029
euka
ryot
ic tr
ansl
atio
n el
onga
tion
fact
or 2
Hom
o sa
pien
sgi
|450
3483
34/8
346
100
9624
6/6.
4186
466/
6.62
T031
*ac
onita
se 1
Hom
o sa
pien
sgi
|865
9555
18/7
323
100
9885
0/6.
2384
037/
6.25
T032
*ac
onita
se 1
Hom
o sa
pien
sgi
|865
9555
15/5
419
100
9885
0/6.
2384
221/
5.17
T033
*he
at sh
ock
prot
ein
HSP
90-
alph
a (H
SP 8
6)Eq
uus c
abal
lus
gi|1
7865
490
19/7
128
200
8344
5/5.
080
786/
5.78
T034
*he
at sh
ock
prot
ein
HSP
90-
alph
a (H
SP 8
6)Eq
uus c
abal
lus
gi|1
7865
490
22/8
835
200
8344
5/5.
081
141/
5.69
T035
*he
at sh
ock
prot
ein
HSP
90-
alph
a (H
SP 8
6)H
omo
sapi
ens
gi|1
2367
820
/69
3220
085
020/
4.94
8239
5/5.
26
T036
*he
at sh
ock
prot
ein
HSP
90-
alph
a (H
SP 8
6)Eq
uus c
abal
lus
gi|1
7865
490
22/4
531
100
8344
5/5.
082
576/
5.10
T037
*he
at sh
ock
prot
ein
HSP
90-
alph
a (H
SP 8
6)H
omo
sapi
ens
gi|1
2367
822
/61
3010
085
020/
4.94
8239
5/5.
02
T038
*he
at sh
ock
90kD
a pr
otei
n 1,
alp
haH
omo
sapi
ens
gi|1
3129
150
26/6
734
100
8500
6/4.
9482
757/
4.82
T039
*ac
onita
se (E
.C.4
.2.1
.3)
Sus s
crof
agi
|231
108
29/6
345
100
8330
8/7.
2680
080/
7.13
T040
*ac
onita
se (E
.C.4
.2.1
.3)
Sus s
crof
agi
|231
108
33/8
048
100
8330
8/7.
2680
080/
7.05
T041
*ac
onita
se (E
.C.4
.2.1
.3)
Sus s
crof
agi
|231
108
31/6
749
200
8330
8/7.
2680
080/
6.98
T042
*ac
onita
se (E
.C.4
.2.1
.3)
Sus s
crof
agi
|231
108
26/5
443
200
8330
8/7.
2680
256/
6.90
T043
*ac
onita
se (E
.C.4
.2.1
.3)
Sus s
crof
agi
|231
108
18/4
829
300
8330
8/7.
2680
256/
6.80
T044
*tra
nsfe
rrin
Sus s
crof
agi
|833
800
43/9
563
100
7895
4/6.
7377
320/
6.54
T045
*tra
nsfe
rrin
Sus s
crof
agi
|833
800
32/5
453
200
7895
4/6.
7377
151/
6.44
T046
*tra
nsfe
rrin
Sus s
crof
agi
|833
800
41/8
065
200
7895
4/6.
7376
645/
6.35
T047
*tra
nsfe
rrin
gSu
s scr
ofa
gi|8
3380
026
/47
4510
078
954/
6.73
7647
8/6.
29
T048
*tra
nsfe
rrin
Sus s
crof
agi
|833
800
29/4
549
100
7895
4/6.
7376
478/
6.25
T049
*tra
nsfe
rrin
Sus s
crof
agi
|833
800
17/8
333
100
7895
4/6.
7376
478/
6.18
T050
*tra
nsfe
rrin
Sus s
crof
agi
|833
800
38/8
560
100
7895
4/6.
7376
310/
6.10
T051
*tra
nsfe
rrin
Sus s
crof
agi
|833
800
33/7
652
100
7895
4/6.
7376
645/
6.03
T052
villi
n 2
[ezr
in]
Bos t
auru
sgi
|278
0635
117
/59
2620
068
832/
6.06
7766
0/5.
79
T053
*th
imet
olig
opep
tidas
e (E
ndop
eptid
ase
24.1
5)Su
s scr
ofa
gi|1
3465
1817
/35
2710
078
950/
5.82
7532
7/5.
79
T054
heat
shoc
k 70
kDa
prot
ein
5 (g
luco
se-r
egul
ated
Hom
o sa
pien
sgi
|165
0723
735
/65
5210
072
402/
5.07
7564
4/4.
85
prot
ein,
78k
Da)
T055
heat
shoc
k 70
kDa
prot
ein
5 (g
luco
se-r
egul
ated
prot
ein,
78k
Da)
Hom
o sa
pien
sgi
|165
0723
732
/67
4810
072
402/
5.07
7581
0/4.
83
T056
heat
shoc
k 70
kDa
prot
ein
5 (g
luco
se-r
egul
ated
prot
ein,
78k
Da)
Hom
o sa
pien
sgi
|165
0723
733
/71
5310
072
402/
5.07
7597
6/4.
80
T058
guan
ine
mon
opho
spha
te sy
nthe
tase
Hom
o sa
pien
sgi
|450
4035
15/4
327
100
7740
8/6.
4272
078/
6.36
T059
guan
ine
mon
opho
spha
te sy
nthe
tase
Hom
o sa
pien
sgi
|450
4035
17/6
927
100
7740
8/6.
4272
230/
6.28
T060
*te
stis
-spe
cific
hea
t sho
ck p
rote
in-r
elat
ed g
ene
hst7
0Ra
ttus n
orve
gicu
sgi
|111
7791
026
/83
4510
069
771/
5.44
7102
7/5.
31
T061
heat
shoc
k co
gnat
e 71
kD
a pr
otei
nC
rice
tulu
s gri
seus
gi|1
2364
723
/92
4510
070
989/
5.24
7117
6/5.
20
T062
heat
shoc
k co
gnat
e 71
kD
a pr
otei
nC
rice
tulu
s gri
seus
gi|1
2364
724
/69
4410
070
989/
5.24
7147
6/5.
14
T063
heat
shoc
k 70
kDa
prot
ein
8 is
ofor
m 2
Hom
o sa
pien
sgi
|242
3468
613
/51
3910
053
598/
5.62
7177
6/5.
04
T064
seru
m a
lbum
in p
recu
rsor
Sus s
crof
agi
|113
578
14/7
636
100
7136
2/5.
9271
776/
4.98
T065
albu
min
Sus s
crof
agi
|164
318
11/4
626
100
7134
8/5.
9271
776/
4.93
T068
seru
m a
lbum
in p
recu
rsor
Sus s
crof
agi
|113
578
14/7
134
100
7136
2/5.
9270
285/
7.23
T069
seru
m a
lbum
in p
recu
rsor
Sus s
crof
agi
|113
578
14/2
432
100
7136
2/5.
9270
138/
7.10
T070
com
plem
ent c
ompo
nent
C3
Sus s
crof
agi
|118
6993
127
/95
2110
018
8229
/6.0
968
537/
7.01
T071
seru
m a
lbum
in p
recu
rsor
Sus s
crof
agi
|113
578
13/4
328
100
7136
2/5.
9269
991/
6.95
T073
heat
shoc
k pr
otei
n 70
test
is v
aria
ntH
omo
sapi
ens
gi|3
4618
6616
/79
2810
070
792/
5.76
7102
7/6.
78
T074
seru
m a
lbum
in p
recu
rsor
Sus s
crof
agi
|113
578
18/7
444
100
7136
2/5.
9269
991/
6.61
T075
*A
TP su
lfury
lase
/APS
kin
ase
isof
orm
SK
2H
omo
sapi
ens
gi|6
4660
6810
/65
4410
030
293/
8.33
6516
9/6.
94
T076
*1.
ATP
sulfu
ryla
se/A
PS k
inas
e is
ofor
m S
K2
Hom
o sa
pien
sgi
|646
6026
11/5
824
100
7049
6/8.
1865
306/
6.89
2. 3
'-pho
spho
aden
osin
e 5'
-pho
spho
sulfa
te sy
ntha
se 2
Hom
o sa
pien
sgi
|344
4723
113
/58
2410
070
027/
8.18
6530
6/6.
89
T077
*5-
amin
oim
idaz
ole-
4-ca
rbox
amid
e rib
onuc
leot
ide
form
yltra
nsfe
rase
/IMP
cycl
ohyd
rola
se
Hom
o sa
pien
sgi
|201
2745
411
/61
2510
065
089/
6.27
6476
0/6.
70
T079
*st
ress
-indu
ced-
phos
phop
rote
in 1
(Hsp
70/H
sp90
-
orga
nizi
ng p
rote
in)
Hom
o sa
pien
sgi
|580
3181
21/6
135
100
6322
7/6.
465
306/
6.40
T080
*1.
Hsp
70/H
sp90
org
aniz
ing
prot
ein
Cri
cetu
lus g
rise
usgi
|274
5838
17/4
328
200
6323
9/6.
465
169/
6.27
2. e
xten
din
Mus
mus
culu
sgi
|881
485
17/4
328
200
6317
0/6.
465
169/
6.27
T081
dihy
drop
yrim
idin
ase
rela
ted
prot
ein-
2 (D
RP-
2)Bo
s tau
rus
gi|3
1220
1817
/42
4510
062
638/
5.95
6421
9/6.
03
T082
pyru
vate
kin
ase,
M2
isoz
yme
Ory
ctol
agus
cun
icul
usgi
|403
3427
18/1
0333
100
5843
8/7.
9662
097/
7.84
T083
pyru
vate
kin
ase;
ATP
:pyr
uvat
e 2-
o-
phos
phot
rans
fera
se
Ory
ctol
agus
cun
icul
usgi
|262
3945
13/3
929
100
5830
7/7.
9661
967/
7.52
T084
pyru
vate
kin
ase
3 is
ofor
m 2
Hom
o sa
pien
sgi
|332
8642
29/
2617
100
5853
8/7.
6061
320/
7.31
T086
cata
lase
Sus s
crof
agi
|997
2724
14/7
438
100
5756
2/6.
2661
192/
6.47
T087
fruc
tose
-6-p
hosp
hate
-2-k
inas
e/fr
ucto
se-2
,6-
bisp
hosp
hata
se
Ory
za s
ativ
agi
|182
5145
913
/65
2610
082
739/
6.19
6144
9/6.
39
T089
imm
unog
lobu
lin g
amm
a-ch
ain
Sus s
crof
agi
|164
507
9/37
3110
051
887/
6.71
5486
3/7.
55
T090
imm
unog
lobu
lin g
amm
a-ch
ain
Sus s
crof
agi
|164
507
8/37
2810
051
887/
6.71
5451
9/7.
30
T092
Ig g
amm
a 4
chai
n co
nsta
nt re
gion
(fra
gmen
t)Su
s scr
ofa
gi|2
1365
169/
6361
100
3135
4/8.
6854
634/
6.99
T093
imm
unog
lobu
lin g
amm
a-ch
ain
Sus s
crof
agi
|164
507
9/33
2620
051
887/
6.71
5463
4/6.
93
T094
imm
unog
lobu
lin g
amm
a-ch
ain
Sus s
crof
agi
|164
507
9/39
2413
051
887/
6.71
5406
3/6.
76
T095
imm
unog
lobu
lin g
amm
a-ch
ain
Sus s
crof
agi
|164
507
10/4
231
150
5188
7/6.
7155
326/
6.65
T096
alde
hyde
deh
ydro
gena
se fa
mily
6, s
ubfa
mily
A1
Mus
mus
culu
sgi
|232
7111
510
/41
2215
058
438/
8.25
5626
3/6.
60
T097
imm
unog
lobu
lin g
amm
a-ch
ain
Sus s
crof
agi
|164
507
8/33
2880
5188
7/6.
7153
950/
6.60
T098
imm
unog
lobu
lin g
amm
a-ch
ain
Sus s
crof
agi
|164
507
6/15
1950
5188
7/6.
7155
094/
6.47
T099
succ
inyl
-CoA
:3-k
etoa
cid-
coen
zym
e A
tran
sfer
ase,
mito
chon
dria
l pre
curs
or (S
ucci
nyl C
oA:3
-oxo
acid
CoA
-tran
sfer
ase)
Sus s
crof
agi
|249
2999
12/5
830
100
5677
0/8.
4757
698/
6.41
T100
dihy
drol
ipoa
mid
e de
hydr
ogen
ase
Mes
ocri
cetu
s aur
atus
gi|2
8070
943
8/27
1912
051
205/
6.94
5782
0/6.
35
T101
phos
phog
lyce
rate
deh
ydro
gena
seH
omo
sapi
ens
gi|2
3308
577
7/23
1410
057
356/
6.29
5638
1/6.
37
T103
chai
n A
, suc
cina
te:c
oenz
yme-
A tr
ansf
eras
eSu
s scr
ofa
gi|2
7574
274
8/35
2910
052
482/
6.67
5782
0/6.
24
T104
*se
leni
um b
indi
ng p
rote
in 1
Hom
o sa
pien
sgi
|163
0655
012
/42
2810
052
928/
5.93
5614
5/6.
24
T105
*gl
ucos
e-6-
phos
phat
e de
hydr
ogen
ase
Hom
o sa
pien
sgi
|182
869
10/6
635
130
4223
5/6.
255
910/
6.17
T106
*se
leni
um b
indi
ng p
rote
in 1
Hom
o sa
pien
sgi
|163
0655
013
/66
3510
052
928/
5.93
5383
7/6.
20
T107
*se
leni
um b
indi
ng p
rote
in 1
Hom
o sa
pien
sgi
|163
0655
016
/68
3820
052
928/
5.93
5338
6/6.
10
T108
*se
leni
um b
indi
ng p
rote
in 1
Hom
o sa
pien
sgi
|163
0655
015
/60
3610
052
928/
5.93
5327
4/5.
99
T109
*se
leni
um b
indi
ng p
rote
in 1
Hom
o sa
pien
sgi
|163
0655
013
/100
3710
052
928/
5.93
5383
7/5.
84
T110
*se
leni
um b
indi
ng p
rote
in 1
Hom
o sa
pien
sgi
|163
0655
015
/107
4210
052
928/
5.93
5305
1/5.
79
T111
fasc
in 1
Hom
o sa
pien
sgi
|136
2341
511
/61
2910
055
151/
6.84
5440
5/5.
74
T112
villi
n 2;
ezr
inBo
s tau
rus
gi|2
7806
351
12/3
417
100
6883
2/6.
0658
185/
5.83
T113
1. p
rote
in d
isul
fide-
isom
eras
e (E
C 5
.3.4
.1) E
R60
prec
urso
r
Hom
o sa
pien
sgi
|743
7388
13/4
324
200
5716
0/5.
9858
063/
5.69
2. p
hosp
holip
ase
C-a
lpha
Hom
o sa
pien
sgi
|303
618
12/4
321
200
5706
5/6.
2358
063/
5.69
T114
1. p
rote
in d
isul
fide-
isom
eras
e (E
C 5
.3.4
.1) E
R60
prec
urso
r
Hom
o sa
pien
sgi
|743
7388
20/4
937
200
5716
0/5.
9858
063/
5.69
2. p
hosp
holip
ase
C-a
lpha
Hom
o sa
pien
sgi
|303
618
17/4
932
200
5706
5/6.
2358
063/
5.69
T115
MH
C D
R-b
eta-
1 ch
ain.
Hom
o sa
pien
sgi
|386
932
5/19
3410
025
057/
7.22
5383
7/5.
61
T118
heat
shoc
k pr
otei
n 60
(liv
er)
Rattu
s nor
vegi
cus
gi|1
1560
024
16/4
828
200
6109
8/5.
9160
553/
5.14
T119
Cha
pero
nin
Gro
EL (H
SP60
fam
ily)
Burk
hold
eria
fung
orum
gi|2
2984
788
9/39
2512
063
427/
6.29
6119
2/5.
04
T120
alph
a-1-
antit
ryps
in p
recu
rsor
(alp
ha-1
pro
teas
e
inhi
bito
r)
Sus s
crof
agi
|170
3026
10/5
626
100
4744
9/5.
5462
228/
4.94
T125
*se
rine/
thre
onin
e pr
otei
n ph
osph
atas
e 2A
, 65
KD
A
regu
lato
ry su
buni
t A, a
lpha
isof
orm
(PP2
A, s
ubun
it A
,
PR65
-alp
ha is
ofor
m)
Hom
o sa
pien
sgi
|231
443
17/6
435
100
6598
0/4.
9663
548/
4.83
T126
calre
ticul
in, b
rain
isof
orm
1 p
recu
rsor
(CR
P55)
Bos t
auru
sgi
|285
5877
013
/52
3720
048
180/
4.31
6489
6/4.
70
T128
alph
a-2-
HS-
glyc
opro
tein
pre
curs
or (F
etui
n-A
)Su
s scr
ofa
gi|2
3146
79/
3035
150
3919
9/5.
561
320/
4.65
T129
alph
a-2-
HS-
glyc
opro
tein
pre
curs
or (F
etui
n-A
)Su
s scr
ofa
gi|2
3146
78/
2930
200
3919
9/5.
561
449/
4.61
T130
alph
a en
olas
eTr
ache
mys
scri
pta
gi|4
4163
8511
/83
3610
040
532/
5.35
5638
1/5.
27
T131
*al
pha-
tubu
linM
nem
iops
is le
idyi
gi|3
2967
408
10/4
032
200
4322
1/6.
0756
619/
5.19
T132
*al
pha-
tubu
lin 1
Cio
na in
test
inal
isgi
|216
6722
711
/84
3810
044
574/
5.96
5650
0/5.
14
T133
*al
pha-
tubu
linH
omo
sapi
ens
gi|3
7492
17/6
155
100
5081
0/5.
0257
577/
5.02
T134
*tu
bulin
, alp
ha 2
Mus
mus
culu
sgi
|347
4033
520
/70
5410
050
804/
4.94
5745
7/4.
90
T135
*tu
bulin
alp
ha c
hain
(fra
gmen
t)G
allu
s gal
lus
gi|7
1575
18/5
559
100
4625
6/5.
055
094/
4.89
T136
*tu
bulin
, bet
a, 2
Hom
o sa
pien
sgi
|517
4735
27/7
973
100
5025
5/4.
7955
326/
4.78
T137
prol
yl 4
-hyd
roxy
lase
, bet
a po
lype
ptid
eM
us m
uscu
lus
gi|1
4250
251
15/5
231
200
5742
2/4.
7756
976/
4.68
T138
alph
a en
olas
eTr
ache
mys
scri
pta
gi|4
4163
859/
6338
100
4053
2/5.
3554
177/
4.63
T139
euka
ryot
ic tr
ansl
atio
n el
onga
tion
fact
or 1
alp
ha 1
Hom
o sa
pien
sgi
|450
3471
12/6
233
100
5045
1/9.
149
813/
8.84
T140
1. fe
rred
oxin
redu
ctas
eM
us m
uscu
lus
gi|6
6797
6710
/38
2410
054
568/
8.91
4813
4/8.
44
2. c
hain
A, a
dren
odox
in re
duct
ase
of m
itoch
ondr
ial
P450
Sys
tem
s
Bos t
auru
sgi
|493
0018
9/38
2410
050
550/
6.8
4813
4/8.
44
T141
chai
n A
, 4-a
min
obut
yrat
e-am
inot
rans
fera
seSu
s scr
ofa
gi|7
2459
6412
/33
3410
053
807/
7.2
4960
4/7.
10
T142
*tu
bulin
, bet
a, 2
Hom
o sa
pien
sgi
|517
4735
26/7
370
100
5025
5/4.
7948
881/
6.97
T143
*tu
bulin
, bet
a, 2
Hom
o sa
pien
sgi
|239
5813
321
/45
6310
050
264/
4.83
4908
7/6.
77
T144
alph
a en
olas
e (2
-pho
spho
-D-g
lyce
rate
hyd
ro-ly
ase)
Mus
mus
culu
sgi
|136
3777
69/
4132
100
4745
3/6.
3749
813/
6.46
T145
alph
a en
olas
e (2
-pho
spho
-D-g
lyce
rate
hyd
ro-ly
ase)
Mus
mus
culu
sgi
|136
3777
614
/52
3610
047
453/
6.37
4857
4/6.
28
T146
alph
a en
olas
eSp
heno
don
punc
tatu
sgi
|441
6381
11/4
846
100
4075
7/5.
3448
222/
6.13
T147
alph
a en
olas
eSp
heno
don
punc
tatu
sgi
|441
6381
12/7
947
100
4075
7/5.
3448
310/
5.98
T148
guan
osin
e di
phos
phat
e (G
DP)
dis
soci
atio
n in
hibi
tor 2
Mus
mus
culu
sgi
|667
9985
22/5
747
100
5096
9/5.
9447
266/
5.61
T149
*1.
cA
MP-
depe
nden
t pro
tein
kin
ase
type
I-al
pha
regu
lato
ry c
hain
Bos t
auru
sgi
|125
192
14/4
940
100
4296
3/5.
2749
709/
5.05
2. a
ctin
, bet
aH
omo
sapi
ens
gi|1
4250
401
9/49
3710
041
321/
5.56
4970
9/5.
05
T150
3-ph
osph
ogly
cera
te k
inas
e (p
gk)
Arch
aeog
lobu
s
fulg
idus
DSM
430
4
gi|1
1498
746
10/4
935
100
4536
4/6.
1249
604/
4.99
T153
H+-
trans
porti
ng tw
o-se
ctor
ATP
ase
beta
cha
in,
mito
chon
dria
l (fr
agm
ent)
Rattu
s nor
vegi
cus
gi|9
2350
18/6
648
100
5073
8/4.
950
870/
4.86
T154
retin
obla
stom
a bi
ndin
g pr
otei
n 7
Hom
o sa
pien
sgi
|450
6439
12/5
840
100
4813
2/4.
8951
299/
4.76
T155
*rib
onuc
leas
e in
hibi
tor
Sus s
crof
agi
|132
574
20/4
870
100
5070
2/4.
7647
352/
4.62
T156
*rib
onuc
leas
e in
hibi
tor
Sus s
crof
agi
|132
574
12/5
738
100
5070
2/4.
7646
162/
7.84
T157
*1.
ribo
nucl
ease
inhi
bito
rSu
s scr
ofa
gi|1
3257
414
/68
4410
050
702/
4.76
4615
2/7.
55
2. fu
mar
ate
hydr
atas
e, m
itoch
ondr
ial (
Fum
aras
e)Su
s scr
ofa
gi|1
2060
412
/68
2610
050
149/
7.01
4615
2/7.
55
T158
fum
arat
e hy
drat
ase,
mito
chon
dria
l (Fu
mar
ase)
Sus s
crof
agi
|120
604
12/6
234
100
5014
9/7.
0146
162/
7.36
T159
fum
arat
e hy
drat
ase,
mito
chon
dria
l (Fu
mar
ase)
Sus s
crof
agi
|120
604
11/4
435
100
5014
9/7.
0146
246/
6.99
T160
1. b
ranc
hed
chai
n ke
to a
cid
dehy
drog
enas
e E1
, alp
ha
poly
pept
ide
(map
le sy
rup
urin
e di
seas
e)
Bos t
auru
sgi
|278
0622
911
/53
3110
051
931/
8.62
4491
9/6.
54
2. 2
-oxo
isov
aler
ate
dehy
drog
enas
e al
pha
subu
nit
Mac
aca
fasc
icul
aris
gi|2
3574
708
11/5
335
100
5079
1/8.
7444
919/
6.54
T161
CG
I-47
pro
tein
Hom
o sa
pien
sgi
|770
5763
8/29
2710
046
443/
6.3
4591
0/6.
64
T162
seru
m a
lbum
in p
recu
rsor
Sus s
crof
agi
|113
578
21/4
338
200
7136
2/5.
9244
351/
6.28
T163
trans
latio
n el
onga
tion
fact
or E
F-Tu
pre
curs
orBo
s tau
rus
gi|2
1199
178/
2426
100
4971
8/6.
7544
351/
6.23
T164
aden
osin
e ki
nase
(EC
2.7
.1.2
0)C
rice
tulu
s gri
seus
gi|2
5320
034
8/30
2915
040
563/
6.12
4549
5/6.
08
T165
orni
thin
e am
inot
rans
fera
seXe
nopu
s lae
vis
gi|1
2642
955
9/38
1815
048
561/
6.11
4435
1/5.
88
T167
*te
stis
-spe
cific
pho
spho
glyc
erat
e ki
nase
Equu
s cab
allu
sgi
|219
9856
88/
3125
100
4524
9/8.
6244
512/
8.27
T168
phos
phog
lyce
rate
kin
ase
Cri
cetu
lus g
rise
usgi
|173
0519
12/5
037
100
4493
3/8.
0242
761/
7.87
T169
phos
phog
lyce
rate
kin
ase
1H
omo
sapi
ens
gi|4
5057
6315
/55
4020
044
985/
8.3
4285
6/7.
16
T170
citra
te sy
ntha
se, m
itoch
ondr
ial p
recu
rsor
Sus s
crof
agi
|116
470
13/4
228
100
5188
2/8.
1343
000/
6.98
T172
citra
te sy
ntha
se, m
itoch
ondr
ial p
recu
rsor
Sus s
crof
agi
|116
470
9/40
1780
5188
2/8.
1342
952/
6.87
T173
citra
te sy
ntha
se, m
itoch
ondr
ial p
recu
rsor
Sus s
crof
agi
|116
470
10/2
724
100
5188
2/8.
1343
000/
6.72
T175
phos
phog
lyce
rate
kin
ase
1M
us m
uscu
lus
gi|6
6792
9111
/32
3310
044
907/
7.53
4285
6/6.
61
T176
NA
DP-
depe
nden
t iso
citra
te d
ehyd
roge
nase
Hom
o sa
pien
sgi
|364
1398
13/5
335
100
4694
4/6.
3443
000/
6.55
T177
beta
ine-
-hom
ocys
tein
e s-
met
hyltr
ansf
eras
eSu
s scr
ofa
gi|3
9156
248/
4255
100
3045
6/6.
243
869/
6.49
T178
isoc
itrat
e de
hydr
ogen
ase
[NA
DP]
cyt
opla
smic
(oxa
losu
ccin
ate
deca
rbox
ylas
e)
Mic
rotu
s mex
ican
usgi
|664
7567
16/5
948
100
4699
1/6.
8142
761/
6.37
T180
isoc
itrat
e de
hydr
ogen
ase
1 (N
AD
P+),
solu
ble
Bos t
auru
sgi
|307
9431
813
/54
3210
047
098/
6.13
4280
8/6.
19
T181
isoc
itrat
e de
hydr
ogen
ase
1 (N
AD
P+),
solu
ble
Mus
mus
culu
sgi
|675
4278
9/37
2810
047
030/
6.48
4200
4/6.
22
T182
seru
m a
lbum
in p
recu
rsor
Sus s
crof
agi
|113
578
14/4
732
100
7136
2/5.
9243
000/
5.92
T183
1. g
amm
a-ac
tinM
us m
uscu
lus
gi|8
0956
116
/51
6010
041
335/
5.56
4295
2/5.
78
2. se
rum
alb
umin
pre
curs
orSu
s scr
ofa
gi|1
1357
820
/51
3710
071
362/
5.92
4295
2/5.
78
T184
actin
, cyt
opla
smic
type
8Xe
nopu
s lae
vis
gi|1
7031
2711
/41
4210
042
163/
5.31
4315
7/5.
69
T185
actin
, cyt
opla
smic
type
5G
allu
s gal
lus
gi|1
7031
2110
/66
3910
042
151/
5.3
4355
1/5.
30
T186
AC
TB p
rote
inH
omo
sapi
ens
gi|1
5277
503
14/5
452
100
4053
6/5.
5543
789/
5.23
T187
actin
, bet
aH
omo
sapi
ens
gi|1
4250
401
14/7
652
100
4132
1/5.
5643
789/
5.16
T188
AC
TB p
rote
inH
omo
sapi
ens
gi|1
5277
503
16/5
065
100
4053
6/5.
5543
000/
5.10
T189
cyto
plas
mic
bet
a ac
tinXe
nopu
s lae
vis
gi|3
3481
3117
/47
6610
042
082/
5.3
4300
0/5.
00
T190
1. si
mila
r to
thio
redo
xin
dom
ain
cont
aini
ng 4
Rattu
s nor
vegi
cugi
|348
6837
512
/47
3610
047
248/
5.09
4574
4/5.
00
2. a
ctin
2D
iphy
llobo
thri
um
dend
ritic
um
gi|1
7031
1010
/47
4010
042
031/
5.39
4574
4/5.
00
T192
actin
, bet
aH
omo
sapi
ens
gi|1
4250
401
16/5
761
100
4132
1/5.
5644
512/
4.92
T193
AC
TB p
rote
inH
omo
sapi
ens
gi|1
5277
503
13/4
052
100
4053
6/5.
5544
351/
4.89
T194
1. h
apto
glob
in a
lpha
1S
Sus s
crof
agi
|201
4756
311
/53
3710
039
028/
6.51
4238
1/4.
94
2. a
ctin
, bet
aH
omo
sapi
ens
gi|6
2730
49/
5334
100
4132
1/5.
5642
381/
4.94
T195
AC
TB p
rote
inH
omo
sapi
ens
gi|1
5277
503
11/3
442
100
4053
6/5.
5541
445/
4.93
T197
HSP
A8
prot
ein
Hom
o sa
pien
sgi
|139
3829
711
/24
2410
064
733/
5.36
4219
2/4.
77
T198
heat
shoc
k 70
kd
prot
ein
3Bo
s tau
rus (
cow
)gi
|462
327
12/2
416
100
6949
9/5.
3241
168/
4.79
T199
vim
entin
Cri
cetu
lus g
rise
usgi
|860
908
26/6
766
100
4461
1/4.
7544
593/
4.63
T200
vim
entin
Cri
cetu
lus g
rise
usgi
|860
908
11/3
134
100
4461
1/4.
7543
314/
4.66
T201
aspa
rtate
am
inot
rans
fera
se, m
itoch
ondr
ial p
recu
rsor
(tran
sam
inas
e A
)
Sus s
crof
agi
|112
985
19/5
661
100
4780
5/9.
1440
305/
8.70
T202
fruc
tose
-bis
phos
phat
e al
dola
se (E
C 4
.1.2
.13)
AO
ryct
olag
us c
unic
ulus
gi|6
8184
11/3
938
100
3964
3/8.
440
894/
8.39
T204
aspa
rtate
am
inot
rans
fera
se, c
ytop
lasm
ic (t
rans
amin
ase
A)
Sus s
crof
agi
|112
976
17/2
953
100
4673
1/6.
7341
306/
6.80
T209
sorb
itol d
ehyd
roge
nase
(L-id
itol 2
-deh
ydro
gena
se)
Ovi
s ari
esgi
|118
625
8/41
3710
038
377/
7.26
3990
2/6.
54
T210
*po
ly(r
C) b
indi
ng p
rote
in 1
Hom
o sa
pien
sgi
|675
4994
11/4
250
200
3801
5/6.
6640
621/
6.49
T211
pept
idyl
prol
yl is
omer
ase
D (c
yclo
phili
n D
)Bo
s tau
rus
gi|2
7806
463
10/4
036
100
4099
4/6.
1241
260/
6.39
T220
1. U
NR
-inte
ract
ing
prot
ein
(WD
-40
repe
at p
rote
in P
T-
WD
)
Hom
o sa
pien
sgi
|126
4395
115
/57
5310
038
756/
4.98
3923
9/4.
84
2. se
rine/
thre
onin
e ki
n7as
e re
cept
or a
ssoc
iate
d pr
otei
nM
us m
uscu
lus
gi|6
7556
8214
/57
4810
038
774/
4.99
3923
9/4.
84
T221
stria
ted
mus
cle
alph
a tro
pom
yosi
n (a
a 81
-284
)Ra
ttus n
orve
gicu
sgi
|574
0610
/51
3310
023
589/
4.61
3858
8/4.
69
T222
stria
ted
mus
cle
alph
a tro
pom
yosi
n (a
a 81
-284
)Ra
ttus n
orve
gicu
sgi
|574
068/
3933
130
2358
9/4.
6138
588/
4.63
T223
skel
etal
mus
cle
tropo
myo
sin
Hom
o sa
pien
sgi
|339
958
12/5
746
100
2657
3/4.
6438
631/
4.59
T224
glyc
eral
dehy
de 3
-pho
spha
te d
ehyd
roge
nase
(GA
PDH
)Su
s scr
ofa
dom
estic
agi
|250
6441
15/6
148
200
3604
1/8.
5136
000/
8.50
T225
dehy
drog
enas
e,gl
ycer
alde
hyde
phos
phat
eSu
s scr
ofa
dom
estic
agi
|229
279
14/5
250
200
3591
5/6.
6336
000/
8.62
T226
glyc
eral
dehy
de-3
-pho
spha
te d
ehyd
roge
nase
(pho
spho
ryla
ting)
(EC
1.2
.1.1
2)
Sus s
crof
a do
mes
tica
gi|6
5987
14/5
144
200
3591
4/6.
935
973/
7.78
T227
dehy
drog
enas
e,gl
ycer
alde
hyde
phos
phat
eSu
s scr
ofa
dom
estic
agi
|229
279
9/53
3010
035
915/
6.63
3575
4/7.
28
T230
alco
hol d
ehyd
roge
nase
[NA
DP+
] (al
dehy
de re
duct
ase)
Sus s
crof
agi
|170
3236
9/36
2510
036
801/
6.51
3748
4/6.
61
T231
anne
xin
2C
anis
fam
iliar
isgi
|376
9555
216
/51
4310
038
915/
6.92
3540
1/6.
60
T233
brai
n ac
yl-C
oA h
ydro
lase
isof
orm
hB
AC
Hd
Hom
o sa
pien
sgi
|325
2828
611
/39
3120
036
887/
7.67
3884
7/6.
39
T238
anne
xin
2C
anis
fam
iliar
isgi
|376
9555
219
/68
5210
038
915/
6.92
3542
8/6.
30
T239
aldo
se 1
-epi
mer
ase
Sus s
crof
agi
|116
1154
515
/65
4510
038
020/
6.31
3850
2/6.
18
T240
dim
eric
dih
ydro
diol
deh
ydro
gena
seSu
s scr
ofa
gi|5
7668
9414
/36
4710
037
017/
6.34
3906
4/6.
11
T241
1.se
rpin
Sus s
crof
a do
mes
tica
gi|4
8162
116
/41
5020
042
698/
6.13
3963
5/6.
04
2. L
euko
cyte
ela
stas
e in
hibi
tor (
LEI)
Sus s
crof
agi
|417
185
15/4
145
200
4260
0/5.
9839
635/
6.04
T242
chai
n A
, an
nexi
n 1
Sus s
crof
agi
|133
9961
315
/62
5220
039
005/
6.37
3748
4/6.
12
T245
*si
mila
r to
RN
A-b
indi
ng p
rote
in 1
0M
us m
uscu
lus
gi|3
8074
229
7/40
4710
017
551/
9.67
3750
4/5.
76
T247
aldo
se re
duct
ase
(AR
)Su
s scr
ofa
gi|5
8474
214
/69
5620
036
188/
5.77
3544
5/5.
83
T248
aldo
se re
duct
ase
(AR
)Su
s scr
ofa
gi|5
8474
210
/56
4420
036
188/
5.77
3550
9/5.
71
T249
alph
a en
olas
eTr
ache
mys
scri
pta
eleg
ans
gi|5
3054
2511
/40
3220
047
646/
6.09
3798
9/5.
60
T253
L-la
ctat
e de
hydr
ogen
ase
B c
hain
Sus s
crof
agi
|117
0738
11/5
738
100
3687
4/5.
5735
347/
5.35
T254
sene
scen
ce m
arke
r pro
tein
-30
(SM
P-30
)Bo
s tau
rus
gi|1
3633
941
10/4
333
200
3385
7/5.
5435
320/
5.27
T255
inor
gani
c py
roph
osph
atas
eH
omo
sapi
ens
gi|1
1056
044
11/4
938
100
3309
5/5.
5435
699/
5.22
T257
serin
e/th
reon
ine
prot
ein
phos
phat
ase
2A, c
atal
ytic
subu
nit,
beta
isof
orm
(PP2
A-b
eta)
Sus s
crof
agi
|135
2666
8/43
3715
034
159/
5.46
3553
6/4.
93
T260
*Tp
m2
prot
ein
Mus
mus
culu
sgi
|165
0792
517
/61
3615
035
175/
5.26
3748
4/4.
56
T261
*Tp
m2
prot
ein
Mus
mus
culu
sgi
|165
0792
513
/52
3520
035
175/
5.26
3438
9/5.
17
T263
*sp
erm
idin
e sy
ntha
se, p
utre
scin
e
amin
opro
pyltr
ansf
eras
e, P
APT
(EC
2.5
.1.6
)
Rattu
s sp.
gi|1
1679
997/
2722
5034
332/
6.46
3373
9/5.
09
T264
leuc
ine
zipp
er tr
ansc
riptio
n fa
ctor
-like
1H
omo
sapi
ens
gi|8
9803
6912
/44
2920
034
627/
5.37
3345
7/4.
99
T265
1. le
ucin
e zi
pper
tran
scrip
tion
fact
or-li
ke 1
Hom
o sa
pien
sgi
|898
0369
11/4
132
100
3462
7/5.
3733
534/
4.95
2. si
mila
r to
phos
phol
ipas
e A
2O
ikop
leur
a di
oica
gi|1
8029
288
7/41
5210
019
191/
8.7
3353
4/4.
95
T266
1. c
ytos
kele
ton-
asso
ciat
ed p
rote
in 1
Sus s
crof
agi
|245
2834
610
/41
4310
027
729/
5.23
3302
6/5.
02
2. 3
4-kD
a su
buni
t of R
NA
pol
ymer
ase
III (
C);
Rpc
34p
Sacc
haro
myc
es
cere
visi
ae
gi|6
3243
308/
4132
100
3628
3/5.
2633
026/
5.02
T267
chai
n A
, ann
exin
VH
omo
sapi
ens
gi|8
0918
517
/60
5320
035
840/
4.94
3315
2/4.
80
T268
anne
xin
5H
omo
sapi
ens
gi|9
9992
69/
4234
150
3597
1/4.
9434
811/
4.61
T269
skel
etal
mus
cle
tropo
myo
sin
Hom
o sa
pien
sgi
|339
956
12/5
335
200
3041
9/4.
6434
337/
4.61
T270
*pr
olife
ratin
g ce
ll nu
clea
r ant
igen
;•
Hom
o sa
pien
sgi
|450
5641
7/19
3810
029
092/
4.57
3407
6/4.
58
T273
chlo
ride
intra
cellu
lar c
hann
el p
rote
in 1
Ory
ctol
agus
cun
icul
usgi
|242
1154
911
/51
4910
027
250/
5.17
3142
8/5.
04
T274
tropo
myo
sin
3H
omo
sapi
ens
gi|2
4119
203
13/3
542
100
2924
3/4.
7531
741/
4.62
T275
TPM
4-A
LK fu
sion
onc
opro
tein
type
2H
omo
sapi
ens
gi|1
0441
386
12/5
634
100
2757
0/4.
7731
887/
4.58
T276
Ran
-bin
ding
pro
tein
1H
omo
sapi
ens
gi|4
7939
632
7/29
3470
2150
3/6.
1230
330/
4.81
T277
myo
-inos
itol m
onop
hosp
hata
seSu
s scr
ofa
gi|3
3775
119/
5730
100
3045
5/4.
9230
574/
4.68
T278
tyro
sine
3/tr
ypto
phan
5 -m
onoo
xyge
nase
act
ivat
ion
prot
ein,
thet
a po
lype
ptid
e
Hom
o sa
pien
sgi
|580
3227
13/6
147
100
2803
2/4.
6829
927/
4.67
T279
1. p
roba
ble
LysR
-fam
ily tr
ansc
riptio
nal r
egul
ator
Bord
etel
la
bron
chis
eptic
a
gi|3
3601
721
9/55
4310
035
094/
9.59
2950
2/4.
68
2. g
lyce
rol-3
-pho
spha
te d
ehyd
roge
nase
Ther
mot
oga
mar
itim
agi
|156
4462
88/
5539
100
3629
7/6.
4929
502/
4.68
T282
chap
eron
in c
onta
inin
g TC
P-1
thet
a su
buni
tM
us m
uscu
lus
gi|5
2959
9210
/43
2510
060
044/
5.44
2784
0/5.
10
T283
1. S
-rec
epto
r kin
ase
Arab
idop
sis l
yrat
agi
|249
6538
58/
4732
100
2495
1/5.
1928
417/
4.91
2. R
AB
3D, m
embe
r RA
S on
coge
ne fa
mily
Rattu
s nor
vegi
cus
gi|1
8034
781
7/47
5210
024
503/
4.75
2841
7/4.
91
T285
ubiq
uitin
car
boxy
l-ter
min
al e
ster
ase
L3 (u
biqu
itin
thio
lest
eras
e)
Hom
o sa
pien
sgi
|517
4741
8/41
4410
026
337/
4.84
2869
8/4.
70
T286
1. h
untin
gtin
inte
ract
ing
prot
ein
2H
omo
sapi
ens
gi|4
8854
178/
4742
100
2250
7/5.
3326
412/
5.02
2. a
lbum
inSu
s scr
ofa
gi|1
6431
811
/47
1910
071
348/
5.92
2641
2/5.
02
T287
apol
ipop
rote
in A
ISu
s scr
ofa
gi|4
2320
116
/42
5010
030
312/
5.38
2681
6/4.
88
T288
1. a
deny
late
kin
ase
isoe
nzym
e 5
(ATP
-AM
P
trans
phos
phor
ylas
e)
Hom
o sa
pien
sgi
|929
6996
6/27
4150
2235
8/5.
3827
226/
4.88
2. R
AB
3D, m
embe
r RA
S on
coge
ne fa
mily
Hom
o sa
pien
sgi
|475
9000
6/27
4150
2448
0/4.
7627
226/
4.88
T290
Pero
xire
doxi
n 2
(Thi
ored
oxin
per
oxid
ase
1)Su
s scr
ofa
gi|1
7177
976/
3436
100
1427
2/4.
725
646/
4.92
T291
MH
C c
lass
II a
ntig
en D
R52
Hom
o sa
pien
sgi
|671
5263
6/26
4110
027
114/
6.49
2557
7/4.
81
T292
tum
or p
rote
in, t
rans
latio
nally
-con
trolle
d 1
Hom
o sa
pien
sgi
|450
7669
8/38
4110
019
697/
4.84
2622
4/4.
70
T295
SET
(Mye
loid
Leu
kem
ia-A
ssoc
iate
d Pr
otei
n)M
us m
uscu
lus
gi|3
9536
176/
1332
100
2434
8/4.
9723
062/
4.71
T296
mal
ate
dehy
drog
enas
e (E
C 1
.1.1
.37)
, mito
chon
dria
lSu
s scr
ofa
dom
estic
agi
|659
3212
/31
5010
033
518/
8.55
3505
1/8.
52
T297
*L-
lact
ate
dehy
drog
enas
e A
cha
in (L
DH
-A)
Sus s
crof
agi
|117
0740
13/5
542
200
3688
0/8.
1834
679/
7.82
T298
*L-
lact
ate
dehy
drog
enas
e A
cha
in (L
DH
-A)
Sus s
crof
agi
|117
0740
10/3
434
100
3688
0/8.
1834
442/
7.50
T299
*17
beta
-est
radi
ol d
ehyd
roge
nase
Sus s
crof
a do
mes
tica
gi|1
0900
9210
/47
3910
031
531/
9.11
3353
4/8.
58
T300
RA
B3D
, mem
ber R
AS
onco
gene
fam
ilyRa
ttus n
orve
gicu
sgi
|180
3478
17/
3042
200
2450
3/4.
7533
406/
8.50
T301
elec
tron
trans
fer f
lavo
prot
ein
alph
a su
buni
t pre
curs
orSu
s scr
ofa
gi|3
5384
836
9/30
4310
033
206/
8.16
3300
1/7.
63
T302
guan
ine
nucl
eotid
e bi
ndin
g pr
otei
n (G
pro
tein
), be
ta
poly
pept
ide
2-lik
e 1
Hom
o sa
pien
sgi
|517
4447
8/45
4215
035
511/
7.6
3250
1/7.
12
T304
Rab
GD
P di
ssoc
iatio
n in
hibi
tor b
eta
Hom
o sa
pien
sgi
|496
0030
9/27
3415
041
413/
8.04
3302
6/6.
73
T305
rhod
anes
e (E
.C.2
.8.1
.1)
Bos t
auru
sgi
|230
290
9/38
2710
033
115/
6.78
3330
4/6.
54
T307
Cha
in B
, Hem
oglo
bin
(Aqu
omet
)Su
s scr
ofa
gi|8
0928
38/
5869
100
1608
2/6.
7633
355/
6.37
T310
1. si
mila
r to
vacu
olar
pro
tein
sorti
ng 2
8D
anio
reri
ogi
|347
8407
59/
5047
100
2571
3/5.
1534
652/
6.07
2. a
polip
opro
tein
B-1
00Ta
xide
a ta
xus
gi|2
9423
171
6/50
6110
011
234/
5.04
3465
2/6.
07
T311
mal
ate
dehy
drog
enas
e (E
C 1
.1.1
.37)
Sus s
crof
agi
|164
543
12/6
850
100
3197
8/6.
1534
363/
5.88
T312
sim
ilar t
o m
KIA
A15
53 p
rote
inH
omo
sapi
ens
gi|3
7551
161
16/6
523
200
9555
6/5.
2933
508/
5.85
T315
carb
onyl
redu
ctas
e/N
AD
P-re
tinol
deh
ydro
gena
seSu
s scr
ofa
gi|1
7298
119
8/47
4110
027
985/
8.88
3174
1/8.
72
T316
corte
xilli
n II
Poly
spho
ndyl
ium
palli
dum
gi|5
1072
1810
/55
2910
051
137/
5.64
3126
4/7.
21
T317
*20
-bet
a-hy
drox
yste
roid
deh
ydro
gena
seSu
s scr
ofa
gi|4
7522
960
17/5
753
100
3201
5/7.
6031
320/
6.59
T318
glut
athi
one
trans
fera
se o
meg
a 1
(GST
O 1
-1)
Sus s
crof
agi
|201
4129
011
/60
4510
027
743/
6.84
3104
2/6.
44
T321
carb
onyl
redu
ctas
e/N
AD
P-re
tinol
deh
ydro
gena
seSu
s scr
ofa
gi|1
7298
119
13/7
150
200
2798
5/8.
8829
110/
8.60
T322
carb
onyl
redu
ctas
e/N
AD
P-re
tinol
deh
ydro
gena
seSu
s scr
ofa
gi|1
7298
119
11/7
659
100
2798
5/8.
8829
188/
8.38
T323
intra
cellu
lar b
eta-
type
car
boni
c an
hydr
ase
Phae
odac
tylu
mgi
|157
7865
49/
5851
100
3134
4/6.
3929
634/
7.75
tric
ornu
tum
T328
cath
epsi
n D
Sus s
crof
agi
|115
719
11/5
033
200
3772
7/6.
3430
904/
6.15
T332
quin
oid
dihy
drop
terid
ine
redu
ctas
eSu
s scr
ofa
gi|2
2094
813
10/7
157
100
2596
9/7.
6828
929/
7.09
T336
phos
phog
lyce
rate
mut
ase
1 (b
rain
)H
omo
sapi
ens
gi|4
5057
5314
/76
5610
028
900/
6.67
2934
5/6.
48
T337
phos
phog
lyce
rate
mut
ase
1 (b
rain
)H
omo
sapi
ens
gi|3
8566
176
7/34
4510
028
916/
6.67
2960
8/6.
41
T338
phos
phog
lyce
rate
mut
ase
1 (b
rain
)H
omo
sapi
ens
gi|3
8566
176
15/7
666
100
2891
6/6.
6729
345/
6.25
T339
*pr
obab
le R
NA
hel
icas
e pr
otei
n D
KFZ
p434
B11
22.1
(fra
gmen
t)
Hom
o sa
pien
sgi
|113
4635
79/
2920
100
7021
9/5.
8827
105/
8.65
T340
1. g
luta
thio
ne S
-tran
sfer
ase
Sus s
crof
agi
|118
5280
10/6
647
100
2549
0/8.
8727
105/
8.56
2. L
TR re
trotra
nspo
sabl
e el
emen
t~pa
rtial
ly su
ppor
ted
by G
ENSC
AN
Ory
zias
latip
esgi
|181
5752
119
/66
1610
017
2940
/8.4
427
105/
8.56
T341
glut
athi
one
S-tra
nsfe
rase
P (G
ST P
1-1)
Sus s
crof
agi
|544
445
11/4
866
100
2371
0/8.
0726
389/
7.79
T343
AK
AP4
50 p
rote
inH
omo
sapi
ens
gi|4
5844
2331
/70
1110
045
4925
/4.9
427
865/
7.26
T345
RA
N (n
ucle
ar im
port/
expo
rt) re
late
dC
aeno
rhab
ditis
eleg
ans
gi|1
7553
976
11/5
250
100
2440
9/6.
9627
494/
6.69
T346
trios
epho
spha
te is
omer
ase
Rattu
s nor
vegi
cus
gi|3
8532
559
12/6
752
100
2730
6/6.
4528
041/
6.59
T347
trios
epho
spha
te is
omer
ase
1H
omo
sapi
ens
gi|1
7389
815
9/58
3610
026
910/
6.45
2739
6/6.
52
T348
glut
athi
one
S-tra
nsfe
rase
, mu
5M
us m
uscu
lus
gi|6
7540
8611
/60
4010
027
016/
6.82
2781
5/6.
34
T349
trios
epho
spha
te is
omer
ase
Rattu
s nor
vegi
cus
gi|3
8532
559
11/5
640
100
2730
6/6.
4527
964/
6.29
T350
hypo
xant
hine
-gua
nine
pho
spho
ribos
yltra
nsfe
rase
(HG
PRT)
Mus
spre
tus
gi|2
4999
389/
4452
100
2429
4/5.
7428
089/
6.11
T351
heat
-sho
ck p
rote
inC
anis
fam
iliar
isgi
|509
7911
67/
3423
100
2292
5/6.
2327
617/
6.08
T352
pero
xire
doxi
n 6
Sus s
crof
agi
|475
2387
010
/49
4410
025
078/
5.73
2829
1/5.
78
T353
non-
sele
nium
glu
tath
ione
pho
spho
lipid
hyd
rope
roxi
de
pero
xida
se (P
HG
Px)
Sus s
crof
agi
|668
9393
15/6
174
100
2507
8/5.
7328
316/
5.71
T354
subs
trate
pro
tein
of m
itoch
ondr
ial A
TP-d
epen
dent
prot
eina
se S
P-22
Bos t
auru
sgi
|627
764
6/30
3140
2170
9/5.
7327
519/
5.76
T356
albu
min
Sus s
crof
agi
|164
318
13/4
919
100
7134
8/5.
9227
226/
5.49
T357
beta
-1,3
-N-a
cety
lglu
cosa
min
yltra
nsfe
rase
bG
nT-4
Hom
o sa
pien
sgi
|135
4052
77/
2331
100
4285
3/9.
4425
922/
5.59
T358
glut
athi
one
trans
fera
se (E
C 2
.5.1
.18)
cla
ss p
i 2Su
s scr
ofa
dom
estic
agi
|108
301
6/24
3910
023
297/
7.30
2645
9/6.
95
T359
hete
roge
nous
nuc
lear
ribo
nucl
eopr
otei
n A
2/B
1M
us m
uscu
lus
gi|3
3294
989/
3328
100
3602
8/8.
6726
342/
6.83
T360
sim
ilar t
o bi
liver
din
redu
ctas
e B
(fla
vin
redu
ctas
e
(NA
DPH
)
Rattu
s nor
vegi
cus
gi|3
4855
391
7/50
4710
022
194/
6.29
2667
3/6.
29
T362
*R
NA
-bin
ding
pro
tein
regu
lato
ry su
buni
tH
omo
sapi
ens
gi|1
4198
257
6/27
5410
020
0508
6.33
2578
3/5.
84
T363
pero
xire
doxi
n 1
Bos t
auru
sgi
|278
0608
111
/57
5510
022
379/
8.81
2553
2/8.
52
T364
pero
xire
doxi
n 1
Mus
mus
culu
sgi
|675
4976
8/31
4710
022
390/
8.26
2546
3/8.
45
T366
phos
phat
idyl
etha
nola
min
e bi
ndin
g pr
otei
nBo
s tau
rus
gi|4
3893
666/
3136
100
2082
8/6.
6224
136/
7.19
T367
supe
roxi
de d
ism
utas
e 2
Sus s
crof
agi
|150
8214
26/
3451
100
1179
5/6.
9524
636/
7.04
T368
shor
t-cha
in d
ehyd
roge
nase
/redu
ctas
e 10
cH
omo
sapi
ens
gi|3
2396
158
8/54
5810
021
570/
11.0
2512
5/6.
86
T369
supe
roxi
de d
ism
utas
e 2
Sus s
crof
agi
|150
8214
27/
3750
100
1179
5/6.
9524
724/
6.63
T370
HN
RPA
2B1
prot
ein
Hom
o sa
pien
sgi
|314
5523
811
/40
3710
028
451/
4.79
2430
9/6.
59
T371
23K
pro
tein
Rattu
s nor
vegi
cus
gi|1
1129
17/
3845
100
2097
2/6.
6224
331/
6.48
T375
cofil
in-1
Ovi
s ari
esgi
|505
1259
010
/34
6510
018
792/
8.16
2024
5/7.
84
T376
dest
rinSu
s scr
ofa
gi|2
6245
928/
3043
100
1979
4/8.
0519
675/
7.55
T377
smoo
th m
uscl
e pr
otei
n SM
22 h
omol
og (f
ragm
ents
)Bo
s tau
rus
gi|5
4311
39/
3246
100
1932
6/6.
9622
453/
6.73
T378
trans
gelin
(Sm
ooth
mus
cle
prot
ein
22-a
lpha
)H
omo
sapi
ens
gi|3
1232
8311
/58
5010
022
653/
8.87
2166
6/6.
74
T379
cofil
in 2
Hom
o sa
pien
sgi
|147
1939
28/
2747
100
1883
9/7.
6620
794/
6.78
T380
trans
gelin
(Sm
ooth
mus
cle
prot
ein
22-a
lpha
)H
omo
sapi
ens
gi|3
1232
8310
/59
5010
022
653/
8.87
2195
8/6.
42
T381
ubiq
uitin
-con
juga
ting
enzy
me
E2L
3, is
ofor
m 1
Hom
o sa
pien
sgi
|314
1845
77/
3253
100
1802
1/8.
6819
258/
8.45
T382
pept
idyl
-pro
lyl c
is-tr
ans i
som
eras
e A
(PPI
ase)
Sus s
crof
agi
|403
3689
8/24
5810
018
086/
8.34
1855
0/8.
03
T383
pept
idyl
prol
yl is
omer
ase
(EC
5.2
.1.8
) ASu
s scr
ofa
dom
estic
agi
|684
027/
3238
100
1795
5/8.
3718
663/
7.87
T384
pept
idyl
-pro
lyl c
is-tr
ans i
som
eras
e A
(PPI
ase)
Sus s
crof
agi
|403
3689
9/64
6610
018
086/
8.34
1855
0/7.
41
T385
nucl
eosi
de-d
ipho
spha
te k
inas
e 1
Mus
mus
culu
sgi
|377
0023
27/
3644
100
1731
1/6.
8418
750/
7.28
T386
pept
idyl
-pro
lyl c
is-tr
ans i
som
eras
e A
(PPI
ase)
Sus s
crof
agi
|403
3689
9/35
5510
018
086/
8.34
1866
6/6.
98
T389
pero
xire
doxi
n 5
Sus s
crof
agi
|103
0533
611
/51
6010
017
484/
5.71
1843
4/6.
09
T390
1. su
pero
xide
dis
mut
ase
[Cu-
Zn]
Sus s
crof
agi
|134
620
9/40
6510
015
922/
6.04
1755
2/5.
82
2. p
erox
iredo
xin
5Su
s scr
ofa
gi|1
0305
336
7/40
4810
017
484/
5.71
1755
2/5.
82
T391
hem
oglo
bin
alph
a ch
ain
Sus s
crof
agi
|122
465
8/30
7810
015
087/
8.76
1392
2/8.
53
T392
hem
oglo
bin
alph
aSu
s scr
ofa
gi|1
2246
56/
1770
100
1506
4/8.
7314
050/
8.02
T394
chai
n B
, hem
oglo
bin
(Aqu
omet
)Su
s scr
ofa
gi|8
0928
310
/48
7410
016
082/
6.76
1519
0/6.
75
T395
chai
n B
, hem
oglo
bin
(Aqu
omet
)Su
s scr
ofa
gi|8
0928
310
/44
6710
016
082/
6.76
1469
2/6.
75
T396
chai
n B
, hem
oglo
bin
(Aqu
omet
)Su
s scr
ofa
gi|8
0928
313
/39
9210
016
082/
6.76
1482
0/6.
52
T397
chai
n B
, hem
oglo
bin
(Aqu
omet
)Su
s scr
ofa
gi|8
0928
311
/48
8810
016
082/
6.76
1759
5/6.
27
T398
chai
n B
, hem
oglo
bin
(Aqu
omet
)Su
s scr
ofa
gi|8
0928
38/
6165
100
1608
2/6.
7615
686/
6.13
T399
trans
thyr
etin
pre
curs
or (P
real
bum
in)
Sus s
crof
agi
|171
7817
8/39
5810
016
185/
6.29
1536
0/6.
41
T401
heat
shoc
k 10
kDa
prot
ein
1 (c
hape
roni
n 10
)H
omo
sapi
ens
gi|4
5045
236/
2751
100
1092
5/8.
8912
312/
8.60
T402
Cha
in B
, Hem
oglo
bin
(Aqu
omet
)Su
s scr
ofa
gi|8
0928
37/
6045
5016
082/
6.76
1480
1/7.
29
T404
stat
hmin
-1Su
s scr
ofa
gi|4
9615
355
6/15
3150
1729
2/5.
7619
816/
5.51
T405
pero
xire
doxi
n 5
Sus s
crof
agi
|103
0533
69/
4849
100
1748
4/5.
7118
700/
5.60
T407
*gl
ia m
atur
atio
n fa
ctor
bet
aBo
s tau
rus
gi|6
9051
6/25
5190
1674
3/5.
1919
570/
4.96
T410
cyto
chro
me
b5, e
ryth
rocy
te sp
lice
form
Sus s
crof
agi
|743
0586
5/23
6510
011
147/
5.14
1885
0/4.
75
T412
cellu
lar r
etin
oic
acid
bin
ding
pro
tein
1H
omo
sapi
ens
gi|4
7580
5211
/37
7910
015
727/
5.30
1566
7/5.
21
T414
actin
2D
uges
ia p
olyc
hroa
gi|3
0464
025/
3180
5083
17/5
.51
1476
5/4.
72
T416
adre
nodo
xin
Sus s
crof
a do
mes
tica
gi|2
2302
25/
1745
100
1310
3/4.
3514
765/
4.42
T417
gale
ctin
-1Su
s scr
ofa
gi|4
7716
872
7/41
6110
014
932/
5.07
1413
1/4.
60
T418
ATP
ase,
Na+
/K+
trans
porti
ng, b
eta
2 po
lype
ptid
eH
omo
sapi
ens
gi|4
5022
798/
4238
100
3371
1/8.
5713
325/
4.73
T419
myo
troph
in; g
ranu
le c
ell d
iffer
entia
tion
prot
ein
Hom
o sa
pien
sgi
|219
5664
56/
2769
100
1305
8/5.
2712
989/
4.89
T421
pept
idyl
-pro
lyl c
is-tr
ans i
som
eras
e B
pre
curs
or
(PPI
ase)
Bos t
auru
sgi
|116
9176
13/3
655
100
2274
4/9.
2321
589/
8.77
T424
FK50
6 bi
ndin
g pr
otei
n 2,
13k
Da
Hom
o sa
pien
sgi
|328
8006
57/
2347
100
1581
0/9.
2415
842/
8.52
T425
prof
ilin
IBo
s tau
rus
gi|1
3097
66/
2948
100
1508
8/8.
515
228/
8.13
T426
trans
gelin
Hom
o sa
pien
sgi
|450
7359
8/32
4110
022
518/
8.56
2180
1/6.
07
T429
AD
P-rib
osyl
atio
n fa
ctor
3H
omo
sapi
ens
gi|4
5022
036/
3051
100
2064
5/6.
8420
536/
5.79
T430
low
mol
ecul
ar w
eigh
t pho
spho
tyro
sine
pro
tein
phos
phat
ase
Sus s
crof
agi
|142
8566
77/
4252
100
1848
0/6.
8120
445/
6.00
T431
low
mol
ecul
ar w
eigh
t pho
spho
tyro
sine
pro
tein
phos
phat
ase
Sus s
crof
agi
|142
8566
78/
3658
100
1848
0/6.
8120
101/
6.13
T434
cyto
chro
me
b5H
omo
sapi
ens
gi|3
5381
85/
2062
100
1113
0/5.
0218
567/
4.82
T435
cyto
chro
me
b5, e
ryth
rocy
te sp
lice
form
Ory
ctol
agus
cun
icul
usgi
|109
195
6/24
7510
011
226/
5.41
1639
9/4.
74
T436
calm
odul
inG
allu
s gal
lus
gi|3
4151
196/
2745
150
1684
2/4.
1019
258/
4.06
T437
calm
odul
in 2
(pho
spho
ryla
se k
inas
e, d
elta
)H
omo
sapi
ens
gi|1
4250
065
7/46
4510
016
826/
4.10
1841
8/4.
06
T438
chai
n A
, hum
an c
o-ch
aper
one
P23
Hom
o sa
pien
sgi
|925
7073
9/56
6420
015
146/
5.09
2329
0/4.
20
T439
UD
P gl
ucos
e py
roph
osph
oryl
ase
Sus s
crof
agi
|475
2278
611
/44
2515
057
089/
7.69
5474
8/7.
61
T440
calre
ticul
inRa
ttus n
orve
gicu
sgi
|385
6597
710
/38
2920
048
137/
/4.3
364
489/
4.29
T441
calre
ticul
in p
recu
rsor
(CR
P55)
Sus s
crof
agi
|170
6140
9/50
6310
012
007/
5.60
6183
7/4.
29
T442
calre
ticul
inRa
ttus n
orve
gicu
sgi
|385
6597
711
/53
2515
048
137/
4.33
5733
6/4.
29
T443
F-ac
tin c
appi
ng p
rote
in b
eta
subu
nit
Hom
o sa
pien
sgi
|482
6659
8/28
3510
030
952/
5.69
3104
2/5.
39
T444
dim
ethy
larg
inin
e di
met
hyla
min
ohyd
rola
se 2
Hom
o sa
pien
sgi
|752
4354
8/37
3410
029
911/
5.66
3024
9/5.
39
T446
pero
xire
doxi
n 6
Sus s
crof
agi
|475
2387
08/
2040
300
2507
8/5.
7328
698/
5.48
T447
ubiq
uitin
-con
juga
ting
enzy
me
E2I2
Dan
io re
rio
gi|3
4849
694
6/27
4310
018
152/
8.87
1934
4/8.
52
# Th
e sp
ot
num
bers
re
fer
to
the
num
bers
la
bele
d in
Fi
g.
1.
* in
dica
tes
the
prot
ein
spot
is
a
test
is-s
peci
fic
prot
ein.
