Patient 2 2/20/04 nose

ATCC 29213

Patient 1 2/28/01 ear

Patient 1 11/19/01 nose

Patient 1 11/28/01 ear drng

Sample

Patient 1 8/27/01 nose

Patient 1 8/27/01 skin

Patient 1 5/31/05 nose

Patient 1 3/10/05 nose

Patient 2 11/21/01 ear cnl

Patient 2 3/7/05 drng

Patient 2 5/31/05 nose

Patient 2 2/6/03 ear drng

Kit Positive Control

Control

Supplementary Figure 1: Rep-PCR Results of Twelve Isolates of S. aureus

a.

Supplementary Figure 2: PFGE Results of Ten MRSA Isolates Digested with Restriction Enzymes SmaI and EagI. a) Agarose gel electrophoresis of macrorestriction digest b) Dendrograms of cluster analysis

Restriction Enzyme: SmaI Restriction Enzyme: Eag1

N 8/01 8/01 11/01 11/01 3/05 5/05 N 11/01 2/03 3/05 5/05 N N 8/01 8/01 11/01 11/01 3/05 5/05 N 11/01 2/03 3/05 5/05 A N

Patient 1 Patient 2 Patient 1 Patient 2

E E E-2 E

N = NCTC 8325 control strainA = ATCC 29213 control strain

kb

674

361

262

175135

S S S S-1 S-2 S S S-1 S-2 S-1 E E E E E-1 E

b.

SmaI EagI