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Supplementary Figures. Supplementary Figure 1. Supplementary Fig. 1 Amino acid sequence alignment of DUF724 domain in plants. Black boxes indicate the identical amino acids, and gray boxes indicate the similar amino acids. ViDuf1. 91. PtDuf1. PtDuf2. 100. AtDuf1. AtDuf3. 90. 100. 97. - PowerPoint PPT Presentation
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Supplementary Figure 1
74ViDuf1.pro 74AtDuf1.pro 74AtDuf2.pro 74AtDuf3.pro 74AtDuf4.pro 74AtDuf5.pro 74AtDuf6.pro 74AtDuf7.pro 75AtDuf8.pro 71AtDuf9.pro 74AtDuf10.pro 60OsDuf1.pro 70OsDuf2.pro 74OsDuf3.pro 77OsDuf4.pro 79OsDuf5.pro
QTMTIMMMMMT AICC
SIVIVVIVVTN QEAQ
LSLELLLLLLI LLLL
PPPPPPPPPPL NPSP
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. ...G
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. ..VQ
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. ..SQ
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. ..SA
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. ..QL
FFFFFFFFFFF NFCF
VAATTVAVEEECDVNT
KKKKKKKKKKKQEKTK
SKKNTKKKKKKVCTPT
SLSLLSSSLLLPISSK
PPPPAPPQRSPNVPPD
IFVFSVFLIIVYLMLT
WWWWWWWWWWWWEWGW
NKKKKKKKEKKSPAKS
TSTTEIMVTTIAPQQA
LYYYFYYLLLLAAIIF
EEEEEEEEEEEGKEKE
TTSMSSTSSSSQNAAM
LDLEDMQMMVMSCMFM
EEEKEEEEEEESEEDE
VLVGVVVVV.V.VVFV
FYFYYFCFF.F.IFVF
QKKKKKKKK.K.NSSR
RSSTRRIVTTT.LKRK
MLVVTVAVVVI.DVED
PPSPPPPPPPPFDPAP
QQHQQQQQQQQVEQDQ
KSSNRSSSSSSHERSE
PPLPPPPPPPPPEPGP
HHHHHHHHHHHSQNSH
FFFFFFFFFFFISFNF
RSSSPSSSSSRTVHTL
PPPPSPPPPPPMPQKP
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.L..N..F.
LLLLLLLLLLLL.LLL
EFFLAFFLVVAF.QIQ
NKEEYEEEEEET.QLQ
CATFTAASSTII.HKF
KKKKHKKERRRLNPKP
EEQEPEEEEEEPPPFE
EDDDSDEEDDDAKEAA
RIFIFFLCSCSPHFRV
RRRRGRRRRRRNHRKR
EEEEEEEEEEE.DEKE
GWGWPGWGMMM.RGGN
LSYSTFTDSSL.QISM
AAAAAAAAAAA.LA.A
IVIVALVIVVV.VL.I
GGGGYGGGGGGSLGKG
NMMMLMMRMMMHELEL
MMMMTMMMMMMPLMSF
VVVVAVVVLLLTEYDW
TTTSATSMTTTTESSS
FFYFFFFFFFFFFFPY
SYFYISYSFPSAVTHI
TCGGDGGSGCCSRNND
LLLLCVLLLLLSSLSA
ILLLVLLLLLLLGALG
EKEEKEEEDEELLEDD
KEKETKEKEEQMLSAA
VVFVVVVVVVVGSITI
AKKKDEKNKKKPQNSS
KDDKGDNNASACRMTK
LLLLMLLLLLLEELVL
RQEQCKQQQQQKENQC
FLALETLVHHHICVPI
DDDDDDDDNDDESHIT
DDVVTVVDDNEDDDRD
PSPSPPSPPSAEEDRS
RSVSKIPIIIREDNKT
SSSSSRSSSSSNEAKK
IKQKEQTSFSSVEVAI
FLLLLLLIFLFFDFAF
GSNGDNGNIIIEREGE
SSSSVSSRSSSERHRK
SLLLYLLILLLKNKLH
143ViDuf1.pro 143AtDuf1.pro 147AtDuf2.pro 143AtDuf3.pro 133AtDuf4.pro 150AtDuf5.pro 143AtDuf6.pro 136AtDuf7.pro 137AtDuf8.pro 133AtDuf9.pro 136AtDuf10.pro 136OsDuf1.pro 135OsDuf2.pro 143OsDuf3.pro 151OsDuf4.pro 136OsDuf5.pro
LSKSRKSDTSSLRMKN
ESDTEDCENNNRDRGT
ASSCTSSCSNSCICST
LLFFFFFFFFFLLISL
VASAKTALACASKSVN
EEEEMEEKEEEEDVEY
LLLLFLLLLLLLNLRL
EEEEEEEEEEEEKEQV
MKKKEKKKKKKEDALE
HHHHQHHHHHHDDDEN
GGGGGGGGGGGGPGGG
FFFFFFFFFFFFFFEF
DNNDDTDNNNNDSDTN
TVVIVVVVVVAVEVHV
KTTAATATKKQRARIQ
PDTTEAATAAVAAHQS
VPPPPPPPPPALRLQL
QLLQLLQRQQQKRRQQ
SSSSSSSSSSLEESLC
RRRRQRRRRRRRYRNK
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.P.K.
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.L.A.
IIIIVIIIIIILFLLL
NSDNLANANSNEFEEN
EKKKTKKKKKKNETDK
LVLVLLMILLLLFLNA
VLLLLLLLLLLVILLL
FPASVASSSSTSPSNQ
ILLLLLLILLLIQLAF
KQKQRKQKRRRKKKNK
DDDVKDDEDGGNPNEL
QKRGMRERRKMHHSVD
QRQRRQRQQQQQYWTR
EALARLATSSSTRSNT
QKYKEKKCKMREGKQC
LKIKSIKAKKKLLIES
KAMVLLALTMMKLQLL
GEEEREEEEDDKNDLA
REEEQEEEEEEKNMPY
TRLRQLRLLLLRDMLR
KKKKKKKKKKKAEKTE
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.P.
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.P.
ECGCGVGADGGRARGK
VLFLMFLVAAALLSFL
EEDEEDEEEEKDGEEK
NKKKKKKEKKKQDKSE
QKEREEKK...FLKVQ
IIMIMMIIVVVMAIVV
METEAKETTTTLYANL
ECNADDAEAAAESQGE
HEEEDEGNEEERGEKK
TEFEYSEDKKKELERQ
HISISSIN...VWISS
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.K.....D.DR.
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.K.....N....
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.H.....A....
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.K..K.....S....
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.A..A.....V....
.
.K..E.....E....
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.Q..Q.....Q....
EEEE.EE....S..D.
KRFM.FG....Q.DW.
TKDQ.GH....K.QN.
KRDK.ET....L.QT.
IFMF.MY....L.RD.
DEEE.EE.EEED.CG.
EEQH.RE.SSSIFAF.
EEKE.KEKVIVMLES.
IFIM.IMRKKKILIE.
YALV.LARAIVKVSV.
EDEEKEEKEEEEGVN.
IFVVLVLYNNNLLLL.
DEKEKKEEKEEEASNS
KRHRKNLERRRLKMSL
KIKKSKKDKKKKEVSS
MIIMLVIIIIILVVLR
IIILVLLVLLLLLRFI
EEEEREEEEEEEKQDG
LKLLCLLLLLLYTLEA
188ViDuf1.pro 189AtDuf1.pro 200AtDuf2.pro 189AtDuf3.pro 144AtDuf4.pro 186AtDuf5.pro 189AtDuf6.pro 182AtDuf7.pro 176AtDuf8.pro 172AtDuf9.pro 175AtDuf10.pro 182OsDuf1.pro 182OsDuf2.pro 196OsDuf3.pro 194OsDuf4.pro 154OsDuf5.pro
QKQKKQKQQQQRSEES
ERRRSKRRRRRECQLR
KQQR.QQQ...KDHT.
RAEA.EQE...KTAA.
ALAE.AVV...ADHT.
LVAV.AVL...SALI.
AALA.LAM...LSFN.
VKKK.EKK...VERS.
SEEE.KEE...EICI.
NKQK.QMALLLKVIC.
KKKK.KKKNNNKCKQ.
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.E..........ND.
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.A..........RN.
.
.E..........A..
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.K..........I..
.
.E..........S..
.
.Q..........Q..
.
.K.........LQ..
EEDESDEVEEEAMMHD
SAAAFAATEEEAKSNE
KAAAETTLMVMNKHRN
DDWDDYDDDDACAADD
SKKKKEKNKKKSQMAS
ERKMEKVEEEEEEEEA
IIIITITIIIIILNSM
AGCVACSAAAAAESDA
AEQEKKGRQQQKQRNK
LMMM
MMMSSSLLLVL
LKEK
EKQKKMQGKAE
STSS
SSSSSSGFVTM
SCCS
RYQCCCDNEEE
AAAA
AAAAAEMVAVL
DEKE
REAAAGDKSAG
ATDT
DMVKKKQHQER
MILI
LILIIIILLI
NDND
GNDVVLEIKS
EQVQ
VQQQQQEAQH
SEEE
EEEQQQSRSL
IILI
LIVLLLFLSM
QKEA
EEQDEDLKME
SDDN
DDNDDNSESK
AEVV
VLVVVMAPTS
REEE
ERDKEKEQEM
QLFL
LHLLLYSQL
DEEE
EEEQEDRRP
FFFF
FFFFFFLFL
EQEI
QQLQQHRSD
RTTT
SAATATLSC
ATIS
TITTTTLV
TVLV
ALAAAAQV
PSSL
SASSSADA
DTAA
AAAAAASA
PPP
PPPPPPIP
Supplementary Figures
Supplementary Fig. 1 Amino acid sequence alignment of DUF724 domain in plants.Black boxes indicate the identical amino acids, and gray boxes indicate the similar amino acids.
Supplementary Figure 2
OsDuf4OsDuf1
OsDuf5
OsDuf3OsDuf2
AtDuf4
AtDuf1
AtDuf3
AtDuf6
AtDuf8
AtDuf9
AtDuf10
AtDuf2
AtDuf5
AtDuf7
PtDuf2
PtDuf1
ViDuf1
100
95
100
100
10097
97100
90
100
91
Supplementary Fig. 2 Phylogenetic analysis of DUF724 proteins in plants.Phylogenetic tree was constructed by deduced full-length amino acid sequences of 18 DUF724 proteins in Arabidopsis, rice, poplar and Vitis vinifera.
Supplementary Figure 3
AtDuf10
AtDuf9
AtDuf8
AtDuf3
AtDuf6
AtDuf1
AtDuf2
AtDuf7
AtDuf5
AtDuf4
calluscell suspensionseedingcotyledonshypocotylradicleinflorescenceflowerpetalsepalstamenpedicelsiliqueseedstemnodeshoot apexcauline leafrosettejuvenile leafadult leafpetiolesenescent leafhypocotylerootsroot tipelongation zoneroot hair zonestele
ge
rmin
ate
d s
ee
ds
eed
lin
gy
ou
ng
ro
se
tte
dd
eve
lop
ed r
os
ett
eb
olt
ing
yo
un
g f
low
erd
eve
lop
ed f
low
er
flo
we
rs a
nd
Sil
iqu
es
mat
ure
sil
iqu
es
AtDuf10AtDuf9AtDuf8AtDuf3AtDuf6AtDuf1 AtDuf2AtDuf7AtDuf5 AtDuf4
Co
lor
scal
e
>=
0%<
5%
>=
50%
<55
%
>=
95%
>=
90%
<95
%
>=
80%
<85
%
>=
70%
<75
%
>=
60%
<65
%
>=
40%
<45
%
>=
30%
<35
%
>=
20%
<25
%
>=
10%
<15
%
Supplementary Fig. 3 The expression patterns of the AtDUF724 genes.The figure was prepared using the data obtained from the public microarray databases through Genevestigator, showing the expression patterns of AtDuf genes in different tissues.
Supplementary Figure 4
(A) (B)
Supplementary Fig. 4 Expression of GAL4 AD-AtDuf and GAL4 BD-AtDuf fusion proteins in yeast two hybrid systemThe protein lysates harvested from yeasts transformed with different constructs were assayed by western blotting with antibody against the HA tag (a) and Myc tag (b). Lane 1, pGADT7-AtDuf5; lane 2, pGADT7-AtDuf7; lane 3, pGADT7-AtDuf3; lane 4, pGBKT7-AtDuf5; lane 5, pGBKT7-AtDuf7; lane 6, pGBKT7-AtDuf3 and MW, molecular weight. Stars indicate the position of individual GAL4 fusion proteins.
95
72
55
43
*
**
HA blot
1 2 3 MW
Myc blot
95
7255
43
*
**
4 5 6 MW