4
Supplementary Figure 1 74 ViD uf1.pro 74 A tD uf1.pro 74 A tD uf2.pro 74 A tD uf3.pro 74 A tD uf4.pro 74 A tD uf5.pro 74 A tD uf6.pro 74 A tD uf7.pro 75 A tD uf8.pro 71 A tD uf9.pro 74 A tD uf10.pro 60 O sDuf1.pro 70 O sDuf2.pro 74 O sDuf3.pro 77 O sDuf4.pro 79 O sDuf5.pro Q T M T I M M M M M T A I C C S I V I V V I V V T N Q E A Q L S L E L L L L L L I L L L L P P P P P P P P P P L N P S P . . . . . . . . . . . . . . G . . . . . . . . . . . . . V Q . . . . . . . . . . . . . S Q . . . . . . . . . . . . . S A . . . . . . . . . . . . . Q L F F F F F F F F F F F N F C F V A A T T V A V E E E C D V N T K K K K K K K K K K K Q E K T K S K K N T K K K K K K V C T P T S L S L L S S S L L L P I S S K P P P P A P P Q R S P N V P P D I F V F S V F L I I V Y L M L T W W W W W W W W W W W W E W G W N K K K K K K K E K K S P A K S T S T T E I M V T T I A P Q Q A L Y Y Y F Y Y L L L L A A I I F E E E E E E E E E E E G K E K E T T S M S S T S S S S Q N A A M L D L E D M Q M M V M S C M F M E E E K E E E E E E E S E E D E V L V G V V V V V . V . V V F V F Y F Y Y F C F F . F . I F V F Q K K K K K K K K . K . N S S R R S S T R R I V T T T . L K R K M L V V T V A V V V I . D V E D P P S P P P P P P P P F D P A P Q Q H Q Q Q Q Q Q Q Q V E Q D Q K S S N R S S S S S S H E R S E P P L P P P P P P P P P E P G P H H H H H H H H H H H S Q N S H F F F F F F F F F F F I S F N F R S S S P S S S S S R T V H T L P P P P S P P P P P P M P Q K P . . . . . . . . L . . N . . F . L L L L L L L L L L L L . L L L E F F L A F F L V V A F . Q I Q N K E E Y E E E E E E T . Q L Q C A T F T A A S S T I I . H K F K K K K H K K E R R R L N P K P E E Q E P E E E E E E P P P F E E D D D S D E E D D D A K E A A R I F I F F L C S C S P H F R V R R R R G R R R R R R N H R K R E E E E E E E E E E E . D E K E G W G W P G W G M M M . R G G N L S Y S T F T D S S L . Q I S M A A A A A A A A A A A . L A . A I V I V A L V I V V V . V L . I G G G G Y G G G G G G S L G K G N M M M L M M R M M M H E L E L M M M M T M M M M M M P L M S F V V V V A V V V L L L T E Y D W T T T S A T S M T T T T E S S S F F Y F F F F F F F F F F F P Y S Y F Y I S Y S F P S A V T H I T C G G D G G S G C C S R N N D L L L L C V L L L L L S S L S A I L L L V L L L L L L L G A L G E K E E K E E E D E E L L E D D K E K E T K E K E E Q M L S A A V V F V V V V V V V V G S I T I A K K K D E K N K K K P Q N S S K D D K G D N N A S A C R M T K L L L L M L L L L L L E E L V L R Q E Q C K Q Q Q Q Q K E N Q C F L A L E T L V H H H I C V P I D D D D D D D D N D D E S H I T D D V V T V V D D N E D D D R D P S P S P P S P P S A E E D R S R S V S K I P I I I R E D N K T S S S S S R S S S S S N E A K K I K Q K E Q T S F S S V E V A I F L L L L L L I F L F F D F A F G S N G D N G N I I I E R E G E S S S S V S S R S S S E R H R K S L L L Y L L I L L L K N K L H 143 ViD uf1.pro 143 A tD uf1.pro 147 A tD uf2.pro 143 A tD uf3.pro 133 A tD uf4.pro 150 A tD uf5.pro 143 A tD uf6.pro 136 A tD uf7.pro 137 A tD uf8.pro 133 A tD uf9.pro 136 A tD uf10.pro 136 O sDuf1.pro 135 O sDuf2.pro 143 O sDuf3.pro 151 O sDuf4.pro 136 O sDuf5.pro L S K S R K S D T S S L R M K N E S D T E D C E N N N R D R G T A S S C T S S C S N S C I C S T L L F F F F F F F F F L L I S L V A S A K T A L A C A S K S V N E E E E M E E K E E E E D V E Y L L L L F L L L L L L L N L R L E E E E E E E E E E E E K E Q V M K K K E K K K K K K E D A L E H H H H Q H H H H H H D D D E N G G G G G G G G G G G G P G G G F F F F F F F F F F F F F F E F D N N D D T D N N N N D S D T N T V V I V V V V V V A V E V H V K T T A A T A T K K Q R A R I Q P D T T E A A T A A V A A H Q S V P P P P P P P P P A L R L Q L Q L L Q L L Q R Q Q Q K R R Q Q S S S S S S S S S S L E E S L C R R R R Q R R R R R R R Y R N K . . . . . . . . . . . . P . K . . . . . . . . . . . . . L . A . I I I I V I I I I I I L F L L L N S D N L A N A N S N E F E E N E K K K T K K K K K K N E T D K L V L V L L M I L L L L F L N A V L L L L L L L L L L V I L L L F P A S V A S S S S T S P S N Q I L L L L L L I L L L I Q L A F K Q K Q R K Q K R R R K K K N K D D D V K D D E D G G N P N E L Q K R G M R E R R K M H H S V D Q R Q R R Q R Q Q Q Q Q Y W T R E A L A R L A T S S S T R S N T Q K Y K E K K C K M R E G K Q C L K I K S I K A K K K L L I E S K A M V L L A L T M M K L Q L L G E E E R E E E E D D K N D L A R E E E Q E E E E E E K N M P Y T R L R Q L R L L L L R D M L R K K K K K K K K K K K A E K T E . . . . . . . . . . . . . . P . . . . . . . . . . . . . . . P . E C G C G V G A D G G R A R G K V L F L M F L V A A A L L S F L E E D E E D E E E E K D G E E K N K K K K K K E K K K Q D K S E Q K E R E E K K . . . F L K V Q I I M I M M I I V V V M A I V V M E T E A K E T T T T L Y A N L E C N A D D A E A A A E S Q G E H E E E D E G N E E E R G E K K T E F E Y S E D K K K E L E R Q H I S I S S I N . . . V W I S S . . . . . K . . . . . D . D R . . . . . . K . . . . . N . . . . . . . . . H . . . . . A . . . . . . K . . K . . . . . S . . . . . . A . . A . . . . . V . . . . . . K . . E . . . . . E . . . . . . Q . . Q . . . . . Q . . . . E E E E . E E . . . . S . . D . K R F M . F G . . . . Q . D W . T K D Q . G H . . . . K . Q N . K R D K . E T . . . . L . Q T . I F M F . M Y . . . . L . R D . D E E E . E E . E E E D . C G . E E Q H . R E . S S S I F A F . E E K E . K E K V I V M L E S . I F I M . I M R K K K I L I E . Y A L V . L A R A I V K V S V . E D E E K E E K E E E E G V N . I F V V L V L Y N N N L L L L . D E K E K K E E K E E E A S N S K R H R K N L E R R R L K M S L K I K K S K K D K K K K E V S S M I I M L V I I I I I L V V L R I I I L V L L V L L L L L R F I E E E E R E E E E E E E K Q D G L K L L C L L L L L L Y T L E A 188 ViD uf1.pro 189 A tD uf1.pro 200 A tD uf2.pro 189 A tD uf3.pro 144 A tD uf4.pro 186 A tD uf5.pro 189 A tD uf6.pro 182 A tD uf7.pro 176 A tD uf8.pro 172 A tD uf9.pro 175 A tD uf10.pro 182 O sDuf1.pro 182 O sDuf2.pro 196 O sDuf3.pro 194 O sDuf4.pro 154 O sDuf5.pro Q K Q K K Q K Q Q Q Q R S E E S E R R R S K R R R R R E C Q L R K Q Q R . Q Q Q . . . K D H T . R A E A . E Q E . . . K T A A . A L A E . A V V . . . A D H T . L V A V . A V L . . . S A L I . A A L A . L A M . . . L S F N . V K K K . E K K . . . V E R S . S E E E . K E E . . . E I C I . N K Q K . Q M A L L L K V I C . K K K K . K K K N N N K C K Q . . . E . . . . . . . . . . N D . . . A . . . . . . . . . . R N . . . E . . . . . . . . . . A . . . . K . . . . . . . . . . I . . . . E . . . . . . . . . . S . . . . Q . . . . . . . . . . Q . . . . K . . . . . . . . . L Q . . E E D E S D E V E E E A M M H D S A A A F A A T E E E A K S N E K A A A E T T L M V M N K H R N D D W D D Y D D D D A C A A D D S K K K K E K N K K K S Q M A S E R K M E K V E E E E E E E E A I I I I T I T I I I I I L N S M A G C V A C S A A A A A E S D A A E Q E K K G R Q Q Q K Q R N K L M M M M M M S S S L L L V L L K E K E K Q K K M Q G K A E S T S S S S S S S S G F V T M S C C S R Y Q C C C D N E E E A A A A A A A A A E M V A V L D E K E R E A A A G D K S A G A T D T D M V K K K Q H Q E R M I L I L I L I I I I L L I N D N D G N D V V L E I K S E Q V Q V Q Q Q Q Q E A Q H S E E E E E E Q Q Q S R S L I I L I L I V L L L F L S M Q K E A E E Q D E D L K M E S D D N D D N D D N S E S K A E V V V L V V V M A P T S R E E E E R D K E K E Q E M Q L F L L H L L L Y S Q L D E E E E E E Q E D R R P F F F F F F F F F F L F L E Q E I Q Q L Q Q H R S D R T T T S A A T A T L S C A T I S T I T T T T L V T V L V A L A A A A Q V P S S L S A S S S A D A D T A A A A A A A A S A P P P P P P P P P I P Supplementary Figures Supplementary Fig. 1 Amino acid sequence alignment of DUF724 domain in plants. Black boxes indicate the identical amino acids, and gray boxes indicate the similar amino acids.

Supplementary Figure 1

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Supplementary Figures. Supplementary Figure 1. Supplementary Fig. 1 Amino acid sequence alignment of DUF724 domain in plants. Black boxes indicate the identical amino acids, and gray boxes indicate the similar amino acids. ViDuf1. 91. PtDuf1. PtDuf2. 100. AtDuf1. AtDuf3. 90. 100. 97. - PowerPoint PPT Presentation

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Page 1: Supplementary Figure 1

Supplementary Figure 1

74ViDuf1.pro 74AtDuf1.pro 74AtDuf2.pro 74AtDuf3.pro 74AtDuf4.pro 74AtDuf5.pro 74AtDuf6.pro 74AtDuf7.pro 75AtDuf8.pro 71AtDuf9.pro 74AtDuf10.pro 60OsDuf1.pro 70OsDuf2.pro 74OsDuf3.pro 77OsDuf4.pro 79OsDuf5.pro

QTMTIMMMMMT AICC

SIVIVVIVVTN QEAQ

LSLELLLLLLI LLLL

PPPPPPPPPPL NPSP

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. ...G

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. ..VQ

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. ..SQ

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. ..SA

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. ..QL

FFFFFFFFFFF NFCF

VAATTVAVEEECDVNT

KKKKKKKKKKKQEKTK

SKKNTKKKKKKVCTPT

SLSLLSSSLLLPISSK

PPPPAPPQRSPNVPPD

IFVFSVFLIIVYLMLT

WWWWWWWWWWWWEWGW

NKKKKKKKEKKSPAKS

TSTTEIMVTTIAPQQA

LYYYFYYLLLLAAIIF

EEEEEEEEEEEGKEKE

TTSMSSTSSSSQNAAM

LDLEDMQMMVMSCMFM

EEEKEEEEEEESEEDE

VLVGVVVVV.V.VVFV

FYFYYFCFF.F.IFVF

QKKKKKKKK.K.NSSR

RSSTRRIVTTT.LKRK

MLVVTVAVVVI.DVED

PPSPPPPPPPPFDPAP

QQHQQQQQQQQVEQDQ

KSSNRSSSSSSHERSE

PPLPPPPPPPPPEPGP

HHHHHHHHHHHSQNSH

FFFFFFFFFFFISFNF

RSSSPSSSSSRTVHTL

PPPPSPPPPPPMPQKP

.

.

.

.

.

.

.

.L..N..F.

LLLLLLLLLLLL.LLL

EFFLAFFLVVAF.QIQ

NKEEYEEEEEET.QLQ

CATFTAASSTII.HKF

KKKKHKKERRRLNPKP

EEQEPEEEEEEPPPFE

EDDDSDEEDDDAKEAA

RIFIFFLCSCSPHFRV

RRRRGRRRRRRNHRKR

EEEEEEEEEEE.DEKE

GWGWPGWGMMM.RGGN

LSYSTFTDSSL.QISM

AAAAAAAAAAA.LA.A

IVIVALVIVVV.VL.I

GGGGYGGGGGGSLGKG

NMMMLMMRMMMHELEL

MMMMTMMMMMMPLMSF

VVVVAVVVLLLTEYDW

TTTSATSMTTTTESSS

FFYFFFFFFFFFFFPY

SYFYISYSFPSAVTHI

TCGGDGGSGCCSRNND

LLLLCVLLLLLSSLSA

ILLLVLLLLLLLGALG

EKEEKEEEDEELLEDD

KEKETKEKEEQMLSAA

VVFVVVVVVVVGSITI

AKKKDEKNKKKPQNSS

KDDKGDNNASACRMTK

LLLLMLLLLLLEELVL

RQEQCKQQQQQKENQC

FLALETLVHHHICVPI

DDDDDDDDNDDESHIT

DDVVTVVDDNEDDDRD

PSPSPPSPPSAEEDRS

RSVSKIPIIIREDNKT

SSSSSRSSSSSNEAKK

IKQKEQTSFSSVEVAI

FLLLLLLIFLFFDFAF

GSNGDNGNIIIEREGE

SSSSVSSRSSSERHRK

SLLLYLLILLLKNKLH

143ViDuf1.pro 143AtDuf1.pro 147AtDuf2.pro 143AtDuf3.pro 133AtDuf4.pro 150AtDuf5.pro 143AtDuf6.pro 136AtDuf7.pro 137AtDuf8.pro 133AtDuf9.pro 136AtDuf10.pro 136OsDuf1.pro 135OsDuf2.pro 143OsDuf3.pro 151OsDuf4.pro 136OsDuf5.pro

LSKSRKSDTSSLRMKN

ESDTEDCENNNRDRGT

ASSCTSSCSNSCICST

LLFFFFFFFFFLLISL

VASAKTALACASKSVN

EEEEMEEKEEEEDVEY

LLLLFLLLLLLLNLRL

EEEEEEEEEEEEKEQV

MKKKEKKKKKKEDALE

HHHHQHHHHHHDDDEN

GGGGGGGGGGGGPGGG

FFFFFFFFFFFFFFEF

DNNDDTDNNNNDSDTN

TVVIVVVVVVAVEVHV

KTTAATATKKQRARIQ

PDTTEAATAAVAAHQS

VPPPPPPPPPALRLQL

QLLQLLQRQQQKRRQQ

SSSSSSSSSSLEESLC

RRRRQRRRRRRRYRNK

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.P.K.

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.

.

.L.A.

IIIIVIIIIIILFLLL

NSDNLANANSNEFEEN

EKKKTKKKKKKNETDK

LVLVLLMILLLLFLNA

VLLLLLLLLLLVILLL

FPASVASSSSTSPSNQ

ILLLLLLILLLIQLAF

KQKQRKQKRRRKKKNK

DDDVKDDEDGGNPNEL

QKRGMRERRKMHHSVD

QRQRRQRQQQQQYWTR

EALARLATSSSTRSNT

QKYKEKKCKMREGKQC

LKIKSIKAKKKLLIES

KAMVLLALTMMKLQLL

GEEEREEEEDDKNDLA

REEEQEEEEEEKNMPY

TRLRQLRLLLLRDMLR

KKKKKKKKKKKAEKTE

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.

.P.

.

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.

.

.P.

ECGCGVGADGGRARGK

VLFLMFLVAAALLSFL

EEDEEDEEEEKDGEEK

NKKKKKKEKKKQDKSE

QKEREEKK...FLKVQ

IIMIMMIIVVVMAIVV

METEAKETTTTLYANL

ECNADDAEAAAESQGE

HEEEDEGNEEERGEKK

TEFEYSEDKKKELERQ

HISISSIN...VWISS

.

.

.

.

.K.....D.DR.

.

.

.

.

.K.....N....

.

.

.

.

.H.....A....

.

.K..K.....S....

.

.A..A.....V....

.

.K..E.....E....

.

.Q..Q.....Q....

EEEE.EE....S..D.

KRFM.FG....Q.DW.

TKDQ.GH....K.QN.

KRDK.ET....L.QT.

IFMF.MY....L.RD.

DEEE.EE.EEED.CG.

EEQH.RE.SSSIFAF.

EEKE.KEKVIVMLES.

IFIM.IMRKKKILIE.

YALV.LARAIVKVSV.

EDEEKEEKEEEEGVN.

IFVVLVLYNNNLLLL.

DEKEKKEEKEEEASNS

KRHRKNLERRRLKMSL

KIKKSKKDKKKKEVSS

MIIMLVIIIIILVVLR

IIILVLLVLLLLLRFI

EEEEREEEEEEEKQDG

LKLLCLLLLLLYTLEA

188ViDuf1.pro 189AtDuf1.pro 200AtDuf2.pro 189AtDuf3.pro 144AtDuf4.pro 186AtDuf5.pro 189AtDuf6.pro 182AtDuf7.pro 176AtDuf8.pro 172AtDuf9.pro 175AtDuf10.pro 182OsDuf1.pro 182OsDuf2.pro 196OsDuf3.pro 194OsDuf4.pro 154OsDuf5.pro

QKQKKQKQQQQRSEES

ERRRSKRRRRRECQLR

KQQR.QQQ...KDHT.

RAEA.EQE...KTAA.

ALAE.AVV...ADHT.

LVAV.AVL...SALI.

AALA.LAM...LSFN.

VKKK.EKK...VERS.

SEEE.KEE...EICI.

NKQK.QMALLLKVIC.

KKKK.KKKNNNKCKQ.

.

.E..........ND.

.

.A..........RN.

.

.E..........A..

.

.K..........I..

.

.E..........S..

.

.Q..........Q..

.

.K.........LQ..

EEDESDEVEEEAMMHD

SAAAFAATEEEAKSNE

KAAAETTLMVMNKHRN

DDWDDYDDDDACAADD

SKKKKEKNKKKSQMAS

ERKMEKVEEEEEEEEA

IIIITITIIIIILNSM

AGCVACSAAAAAESDA

AEQEKKGRQQQKQRNK

LMMM

MMMSSSLLLVL

LKEK

EKQKKMQGKAE

STSS

SSSSSSGFVTM

SCCS

RYQCCCDNEEE

AAAA

AAAAAEMVAVL

DEKE

REAAAGDKSAG

ATDT

DMVKKKQHQER

MILI

LILIIIILLI

NDND

GNDVVLEIKS

EQVQ

VQQQQQEAQH

SEEE

EEEQQQSRSL

IILI

LIVLLLFLSM

QKEA

EEQDEDLKME

SDDN

DDNDDNSESK

AEVV

VLVVVMAPTS

REEE

ERDKEKEQEM

QLFL

LHLLLYSQL

DEEE

EEEQEDRRP

FFFF

FFFFFFLFL

EQEI

QQLQQHRSD

RTTT

SAATATLSC

ATIS

TITTTTLV

TVLV

ALAAAAQV

PSSL

SASSSADA

DTAA

AAAAAASA

PPP

PPPPPPIP

Supplementary Figures

Supplementary Fig. 1 Amino acid sequence alignment of DUF724 domain in plants.Black boxes indicate the identical amino acids, and gray boxes indicate the similar amino acids.

Page 2: Supplementary Figure 1

Supplementary Figure 2

OsDuf4OsDuf1

OsDuf5

OsDuf3OsDuf2

AtDuf4

AtDuf1

AtDuf3

AtDuf6

AtDuf8

AtDuf9

AtDuf10

AtDuf2

AtDuf5

AtDuf7

PtDuf2

PtDuf1

ViDuf1

100

95

100

100

10097

97100

90

100

91

Supplementary Fig. 2 Phylogenetic analysis of DUF724 proteins in plants.Phylogenetic tree was constructed by deduced full-length amino acid sequences of 18 DUF724 proteins in Arabidopsis, rice, poplar and Vitis vinifera.

Page 3: Supplementary Figure 1

Supplementary Figure 3

AtDuf10

AtDuf9

AtDuf8

AtDuf3

AtDuf6

AtDuf1

AtDuf2

AtDuf7

AtDuf5

AtDuf4

calluscell suspensionseedingcotyledonshypocotylradicleinflorescenceflowerpetalsepalstamenpedicelsiliqueseedstemnodeshoot apexcauline leafrosettejuvenile leafadult leafpetiolesenescent leafhypocotylerootsroot tipelongation zoneroot hair zonestele

ge

rmin

ate

d s

ee

ds

eed

lin

gy

ou

ng

ro

se

tte

dd

eve

lop

ed r

os

ett

eb

olt

ing

yo

un

g f

low

erd

eve

lop

ed f

low

er

flo

we

rs a

nd

Sil

iqu

es

mat

ure

sil

iqu

es

AtDuf10AtDuf9AtDuf8AtDuf3AtDuf6AtDuf1 AtDuf2AtDuf7AtDuf5 AtDuf4

Co

lor

scal

e

>=

0%<

5%

>=

50%

<55

%

>=

95%

>=

90%

<95

%

>=

80%

<85

%

>=

70%

<75

%

>=

60%

<65

%

>=

40%

<45

%

>=

30%

<35

%

>=

20%

<25

%

>=

10%

<15

%

Supplementary Fig. 3 The expression patterns of the AtDUF724 genes.The figure was prepared using the data obtained from the public microarray databases through Genevestigator, showing the expression patterns of AtDuf genes in different tissues.

Page 4: Supplementary Figure 1

Supplementary Figure 4

(A) (B)

Supplementary Fig. 4 Expression of GAL4 AD-AtDuf and GAL4 BD-AtDuf fusion proteins in yeast two hybrid systemThe protein lysates harvested from yeasts transformed with different constructs were assayed by western blotting with antibody against the HA tag (a) and Myc tag (b). Lane 1, pGADT7-AtDuf5; lane 2, pGADT7-AtDuf7; lane 3, pGADT7-AtDuf3; lane 4, pGBKT7-AtDuf5; lane 5, pGBKT7-AtDuf7; lane 6, pGBKT7-AtDuf3 and MW, molecular weight. Stars indicate the position of individual GAL4 fusion proteins.

95

72

55

43

**

HA blot

1 2 3 MW

Myc blot

95

7255

43

**

4 5 6 MW