-
41
of MLL-AF9 leukemic cells transduced with Scramble or Ikzf2
shRNAs same as
(B), plated in methylcellulose and counted after 5 days with
n=5. (D) Proliferation
at six days post transduction with n=4 (E) Apoptosis at six days
post
transduction, with n=4. (F) Quantitative PCR of MLL-related
genes in MLL-AF9
leukemic cells transduced with Scramble or Ikzf2 shRNAs, **,
p
-
42
n=9 mice. Weights from spleen (D) and liver (E) from moribund
mice that were
from the primary maintenance experiment, Msi2f/f n=10 and
Msi2Δ/Δ n=9 mice.
*p
-
43
**p
-
44
groups when (177) genes differentially expressed in the Msi2f/f
and Msi2Δ/Δ LSC
RNA-sequencing data is overlapped with RNA profiling from AML
patients
(n=363 patients). Columns represent individual patients and rows
are genes Log
TPM expression normalized by row means. Color bar above gene
rows are
displayed as follows: MSI2 expression above one standard
deviation of the
median in patients were labeled as “High,” below as “Low” and
between as “Mid,”
Cluster numbers separated by colors and mutations as shown.
Cluster-4 patients
are further subdivided with shaded grey for normal bone marrow
controls.
Patients in cluster-5 and Cluster-7 were isolated based on their
differential MSI2
levels see Figure 4. (B) Cluster 5 (C) Cluster 7 from Figure 4E,
Circos plots
indicating relative frequency and pairwise co-occurrence of
mutations in patients.
The length of the arc corresponds to the frequency of mutations
in the first gene,
and the width of the ribbon corresponds to the percentage of
patients who also
had a mutation in the second gene. The number next to the gene
represents the
number of patients.
Supplemental Figure 6.
IGV generated plot showing reads on the Ikzf2 locus from RNA
sequencing data
obtained from LSCs of Msi2f/f n=2 and Msi2Δ/Δ n=2. Sashimi plot
shows the
splicing of Ikzf2.
Supplemental Figure 7
(A) mRNA stability of Ikzf2, Hoxa9 and c-Myc in control and
MSI2O/E cells. Data
of two independent experiments are shown. (B) qPCR of Ikzf2,
Hoxa9 and c-Myc
-
45
in c-Kithigh cells of indicated genotypes Msi2f/f n=3 and
Msi2Δ/Δ n=4. (C)
Immunofluorescence staining of c-MYC in sorted Msi2f/f and
Msi2Δ/Δ LSCs. Cells
were stained with c-MYC–specific Ab (left) and DAPI (middle); a
merged image is
shown (right). Bars, 20 µm. Fluorescence intensity was
quantified and
normalized to the control (three independent experiments; 184
and 418 cells
were quantified per group in total). Means and SEM are shown.
(D)
Representative flow cytometric plot for intracellular staining
of MSI2 and IKZF2 in
c-KitHigh cells (left panel). Frequency of IKZF2High cells in
MLL-AF9 leukemic cells
averaged from Msi2f/f n=4 and Msi2Δ/Δ n=4 (right panel). Means
and S.E.M for B-
D, *, p
-
46
3E and 3G) where majority of Msi2Δ/Δ did not form leukemia.
Supplemental Table 1. HITS-CLIP fold change rank list. K562
UV-cross
linked and RNA-IP rank list is a fold change between FLAG-MSI2
overexpression
and empty vector transduced cells. Negative Fold change
represents background
binding of transcripts that have a FKPM>1 1 read on the
RNA-seq.
Supplemental Table 2. Summary of deletion status of primary
Msi2
deficient leukemia. Samples that had more than 70% deletion in
the gated
GFP+ leukemic cells were considered deleted based on
intracellular flow
cytometry and FKPM>1 read from the RNA-seq.
Supplemental Table 3. Summary of differentially expressed genes
in Msi2f/f
and Msi2Δ/Δ LSCs and Msi2 LSC signature. List of downregulated
genes and
upregulated genes ranked on log2-Fold change= >0.75, p
-
47
Supplemental Table 7. List of Top 1097 HITS-CLIP MSI2 bound
genes and
MLL-AF9 previously annotated gene sets.
Supplemental Table 8. List of the overlap of HITS-CLIP MSI2
bound genes
with MLL-AF9 transcriptional targets for RNA-IP
prioritization.
-
MLLA
F9 ls
k cre-
BMT
36
MLLA
F9 ls
k cre+
BMT
350
1
2
3
Live
r Wei
ght (
g)
C
MLLA
F9 lsk
cre-
BMT3
6
MLLA
F9 lsk
cre+
BMT3
50.0
0.2
0.4
0.6
0.8
1.0
***
Spl
een
Wei
ght (
g)
Msi2f/f
Msi2Δ/Δ
Msi2f/f
*
B
Supplemental Figure 1.
Msi2Δ/Δ
D
**
E Spleen weight
cre-
cre+
0.0
0.2
0.4
0.6
0.8
1.0 ***
Spl
een
Wei
ght (
g)
cre-
cre+
0.0
0.5
1.0
1.5
2.0 **
Live
r Wei
ght (
g)
Liver weight
Msi2f/f
Msi2Δ/Δ
1st p
lating
cre-
1st p
lating
cre+
2nd p
lating
cre-
2nd p
lating
cre+
3rd pl
ating
cre-
3rdpla
ting c
re+
4th pl
ating
cre-
4th pl
ating
cre+
5th pl
ating
cre-
5th pl
ating
cre+
0
1000
2000
3000
4000
# C
olon
ies
Plating # R0 R1 R2 R3 R4
Msi2f/f
Msi2Δ/Δ
A
** *
*** *** **
Leukemic Initiation Leukemic Initiation
Leukemic Maintenance Leukemic Maintenance
-
A
0
10
20
30
40
Initiation
Msi2f/f Msi2Δ/Δ
Supplemental Figure 2.
Diff
eren
tiatio
n
G1 W
T
G1 K
OS W
TS K
O
G2/M
WT
G2/M
KO
0
20
40
60
80
Kit hi maintenance
Perc
enta
ge %
G1 W
T
G1 K
OS W
TS K
O
G2/M
WT
G2/M
KO
0
20
40
60
80
Perc
enta
ge %
Kit hi initiationMaintenance Maintenance
Cell cycle C D
% o
f KitH
igh ce
lls
% o
f KitH
igh ce
lls
G1 S G2/M G1 S G2/M
Msi2Δ/Δ
Msi2f/f
Msi2Δ/Δ
Msi2f/f
0
2
4
6
8
10
Sub
-G1(
%)
Init. Maint.
E
No
of c
olon
ies
0
500
1000
1500
No
of c
ells
(1X
106
cells
)
cre-
cre+
0
1
2
3
*
*
Colony BM F
Msi2f/f
Msi2Δ/Δ
Colony cell number
Msi2f/f B
Msi2Δ/Δ
0
20
40
60
80
100
INITIATION DELETION GMPS 111714
0
20
40
60
80
100
MAINTENANCE DELETION PRIMAR TX 111714
0
20
40
60
80
100
INITIATION DELETION 111714 ***
G
GFP
MSI2
Msi2f/f! Msi2Δ/Δ
Msi2Δ/Δ
Undeleted (7% deletion)
Deleted (93% deletion)
Initiation LSK Initiation GMP Maintenance LSK
MS
I2 %
pos
itive
of c
-KitH
igh
Msi2f/f! Msi2Δ/Δ
Msi2f/f! Msi2Δ/Δ
Msi2f/f! Msi2Δ/Δ
H
MS
I2 %
pos
itive
of c
-KitH
igh
MS
I2 %
pos
itive
of c
-KitH
igh
ns *
I J
Apoptosis
-
0 10 20 30 400
50
100
Survival proportions: Survival of maintenance 111114 136 137
second donor
Time
Per
cent
sur
viva
l LegendLegend
0 100 200 300 400 5000
50
100
Survival proportions: Survival of BMT 187-191 from BMT 149 150
secondary
Time
Per
cent
sur
viva
l wtko
0 100 200 300 400 5000
50
100
Survival proportions: Survival of BMT 84 85 initiation donor 35
36
TimeP
erce
nt s
urvi
val Legend
Legend
0 100 200 300 400 5000
50
100
Time
Per
cent
sur
viva
l
Survival proport: Survival of BMT 59 and BMT 60 initiation donor
1 from 35 and 36
0 50 100 150 200 2500
50
100
Survival proportions: Survival of secondary LSK creer 250
251
Days
Per
cent
sur
viva
l
creER-creER+
Maintenance Secondary #3 Cre-ER
0 100 200 3000
50
100
Time
Perc
ent s
urvi
val
Maintenance Secondary #1
****
**
****
**
**
Initiation Secondary #1 Initiation Secondary #2 Initiation
Secondary #3
****
Sur
viva
l (%
)
Days post transplant
Sur
viva
l (%
)
Days post transplant
Sur
viva
l (%
) Days post transplant
Sur
viva
l (%
)
Days post transplant
Sur
viva
l (%
)
Days post transplant S
urvi
val (
%)
Days post transplant
Sur
viva
l (%
)
Days post transplant
A
E
0
10
20
30
Secondary Tx 319 320 111714
Msi2f/f! Msi2/Δ
MS
I2 %
pos
itive
of
c-K
itHig
h
Deletion of (3F) Msi2pos cells H
Supplemental Figure 3.
0 5 10 15 20 250
50
100
Survival proportions: Survival of BMT 149 150 star initiation
donor 4 149 150
Time
Per
cent
sur
viva
l LegendLegend
Msi2f/f Msi2Δ/Δ
0 100 200 300 400 5000
50
100
time
gmp wtgmpko
Msi2f/f Msi2Δ/Δ
0 100 200 300 400 5000
50
100
time
gmp wtgmpko
B C D
F G Maintenance Secondary #2
**
Initiation Secondary #4
-
WT un
treate
d
WT C
ytokin
e10
WT C
ytokin
e30
KO un
treate
d
KO C
ytokin
e10
KO C
ytokin
e30
0
1
2
3
4
5
6
7
WT un
treate
d
WT C
ytokin
e10
WT C
ytokin
e30
KO un
treate
d
KO C
ytokin
e10
KO C
ytokin
e30
0
1
2
3
4
5
6
WT un
treate
d
WT C
ytokin
e10
WT C
ytokin
e30
KO un
treate
d
KO C
ytokin
e10
KO C
ytokin
e30
0
2
4
6
8
10
12
0 102 103 104 1050 102 103 104 105
0
103
104
105
0 102 103 104 105
0
103
104
105
Msi2f/f - 10 30 - 10 30
IL-3 IL6 SCF:
pERK c-Kit
Mac-1
Msi2f/f! Msi2Δ/Δ
c-KitHigh c-KitHigh
c-KitHigh
A B
Msi2Δ/Δ
- 10 30 - 10 30
Msi2f/f (min)
Msi2Δ/Δ
*** *
C
D
ns
Fold
stim
ulat
ion
* ns
***
*
* *
E
pERK c-KitHigh MLL-AF9
pS6 c-KitHigh MLL-AF9 pSTAT5 c-KitHigh MLL-AF9
Fold
stim
ulat
ion
Fold
stim
ulat
ion
Msi2Δ/Δ
- 10 30 - 10 30
Msi2f/f (min)
Msi2Δ/Δ
- 10 30 - 10 30
Msi2f/f (min)
Supplemental Figure 4.
-
1 2 3 4 5 6 7 8
Supplemental Figure 5.
Low
High
Mid
9
MIDLOWHigh
MSI2 text
relative
row min row max
GSM
6036
78G
SM60
3720
GSM
6037
25G
SM60
3731
GSM
6037
82G
SM60
3818
GSM
6037
94G
SM60
3795
GSM
6037
92G
SM60
3801
GSM
6038
17G
SM60
3806
GSM
6036
35G
SM60
3819
GSM
6037
98G
SM60
3805
GSM
6038
03G
SM60
3800
GSM
6038
07G
SM60
3796
GSM
6038
14G
SM60
3808
GSM
6037
93G
SM60
3815
GSM
6038
04G
SM60
3802
GSM
6037
99G
SM60
3776
GSM
6036
79G
SM60
3705
GSM
6038
94G
SM60
3696
GSM
6039
00G
SM60
3677
GSM
6037
15G
SM60
3816
GSM
6037
34G
SM60
3733
GSM
6037
45G
SM60
3687
GSM
6039
57G
SM60
3944
GSM
6036
91G
SM60
3716
GSM
6038
88G
SM60
3710
GSM
6037
28G
SM60
3713
GSM
6037
29G
SM60
3768
GSM
6039
09G
SM60
3948
GSM
6039
20G
SM60
3694
GSM
6039
33G
SM60
3896
GSM
6038
98G
SM60
3912
GSM
6036
64G
SM60
3886
GSM
6038
95G
SM60
3934
GSM
6037
17G
SM60
3736
GSM
6037
52G
SM60
3774
GSM
6037
38G
SM60
3778
GSM
6037
14G
SM60
3698
GSM
6036
92G
SM60
3690
GSM
6037
70G
SM60
3751
GSM
6037
85G
SM60
3784
GSM
6037
86G
SM60
3788
GSM
6037
87G
SM60
3634
GSM
6038
09G
SM60
3813
GSM
6036
23G
SM60
3638
GSM
6037
73G
SM60
3860
GSM
6036
21G
SM60
3637
GSM
6036
26G
SM60
3775
GSM
6037
79G
SM60
3841
GSM
6036
53G
SM60
3628
GSM
6036
49G
SM60
3812
GSM
6038
30G
SM60
3797
GSM
6038
11G
SM60
3823
GSM
6038
21G
SM60
3631
GSM
6036
29G
SM60
3767
GSM
6037
83G
SM60
3777
GSM
6038
10G
SM60
3824
GSM
6036
57G
SM60
3844
GSM
6036
51G
SM60
3674
GSM
6036
40G
SM60
3639
GSM
6036
27G
SM60
3645
GSM
6036
83G
SM60
3641
GSM
6036
22G
SM60
3835
GSM
6036
25G
SM60
3630
GSM
6038
33G
SM60
3826
GSM
6039
64G
SM60
3836
GSM
6038
65G
SM60
3943
GSM
6038
67G
SM60
3643
GSM
6038
37G
SM60
3861
GSM
6036
65G
SM60
3935
GSM
6039
47G
SM60
3941
GSM
6037
66G
SM60
3958
GSM
6039
40G
SM60
3938
GSM
6038
53G
SM60
3919
GSM
6039
65G
SM60
3863
GSM
6036
58G
SM60
3659
GSM
6036
61G
SM60
3669
GSM
6036
63G
SM60
3742
GSM
6037
26G
SM60
3689
GSM
6037
54G
SM60
3719
GSM
6037
57G
SM60
3847
GSM
6039
15G
SM60
3842
GSM
6039
03G
SM60
3932
GSM
6036
84G
SM60
3916
GSM
6039
01G
SM60
3755
GSM
6038
91G
SM60
3906
GSM
6039
08G
SM60
3675
GSM
6039
59G
SM60
3652
GSM
6038
32G
SM60
3732
GSM
6037
65G
SM60
3680
GSM
6036
62G
SM60
3744
GSM
6036
55G
SM60
3743
GSM
6037
35G
SM60
3647
GSM
6037
21G
SM60
3644
GSM
6037
50G
SM60
3700
GSM
6037
58G
SM60
3869
GSM
6038
99G
SM60
3962
GSM
6038
38G
SM60
3911
GSM
6039
52G
SM60
3646
GSM
6039
53G
SM60
3913
GSM
6039
71G
SM60
3904
GSM
6038
50G
SM60
3897
GSM
6037
37G
SM60
3708
GSM
6039
23G
SM60
3893
GSM
6037
07G
SM60
3704
GSM
6039
26G
SM60
3668
GSM
6038
71G
SM60
3877
GSM
6036
95G
SM60
3960
GSM
6038
72G
SM60
3749
GSM
6038
87G
SM60
3854
GSM
6036
70G
SM60
3939
GSM
6038
70G
SM60
3931
GSM
6038
75G
SM60
3881
GSM
6039
81G
SM60
3970
GSM
6037
11G
SM60
3693
GSM
6039
78G
SM60
3975
GSM
6036
97G
SM60
3633
GSM
6036
60G
SM60
3825
GSM
6038
58G
SM60
3790
GSM
6036
56G
SM60
3831
GSM
6036
50G
SM60
3820
GSM
6038
62G
SM60
3654
GSM
6036
48G
SM60
3843
GSM
6036
36G
SM60
3632
GSM
6037
64G
SM60
3739
GSM
6036
85G
SM60
3921
GSM
6037
91G
SM60
3706
GSM
6037
09G
SM60
3762
GSM
6037
60G
SM60
3740
GSM
6037
69G
SM60
3781
GSM
6037
71G
SM60
3882
GSM
6039
56G
SM60
3845
GSM
6039
14G
SM60
3983
GSM
6037
72G
SM60
3763
GSM
6039
61G
SM60
3946
GSM
6037
46G
SM60
3928
GSM
6036
86G
SM60
3703
GSM
6039
55G
SM60
3907
GSM
6038
89G
SM60
3942
GSM
6037
18G
SM60
3855
GSM
6038
64G
SM60
3857
GSM
6036
24G
SM60
3642
GSM
6038
90G
SM60
3937
GSM
6037
24G
SM60
3980
GSM
6038
49G
SM60
3688
GSM
6039
05G
SM60
3671
GSM
6037
41G
SM60
3918
GSM
6036
76G
SM60
3829
GSM
6037
23G
SM60
3977
GSM
6037
53G
SM60
3846
GSM
6039
22G
SM60
3868
GSM
6039
17G
SM60
3673
GSM
6036
99G
SM60
3780
GSM
6038
22G
SM60
3730
GSM
6036
81G
SM60
3727
GSM
6039
36G
SM60
3930
GSM
6038
85G
SM60
3701
GSM
6038
78G
SM60
3756
GSM
6038
73G
SM60
3759
GSM
6038
40G
SM60
3748
GSM
6038
51G
SM60
3747
GSM
6039
66G
SM60
3848
GSM
6038
56G
SM60
3892
GSM
6038
83G
SM60
3963
GSM
6039
25G
SM60
3969
GSM
6039
51G
SM60
3979
GSM
6039
76G
SM60
3879
GSM
6039
74G
SM60
3929
GSM
6039
67G
SM60
3666
GSM
6039
73G
SM60
3968
GSM
6038
74G
SM60
3982
GSM
6039
49G
SM60
3945
GSM
6039
72G
SM60
3880
GSM
6038
76G
SM60
3950
GSM
6038
84G
SM60
3852
GSM
6037
22G
SM60
3789
GSM
6037
12G
SM60
3902
GSM
6036
67G
SM60
3827
GSM
6038
66G
SM60
3839
GSM
6038
34G
SM60
3702
GSM
6036
72G
SM60
3682
GSM
6039
10G
SM60
3828
GSM
6039
27G
SM60
3924
GSM
6037
61G
SM60
3859
GSM
6039
54
1398
614
742
1855
117
745
1411
115
827
1834
518
171
1846
616
665
1775
418
211
1570
517
228
1903
417
739
1434
316
307
1551
717
974
1741
017
270
1885
518
339
1886
515
429
1394
615
038
1625
320
207
1543
419
211
1769
218
985
1808
617
681
1868
916
772
1924
414
239
1578
015
923
1742
414
559
1821
519
334
1675
819
762
1509
417
389
1960
316
745
1431
420
156
1576
517
197
1934
117
924
1896
815
025
1911
517
384
1943
020
025
1942
915
334
2088
914
787
1977
920
062
2068
515
568
1532
919
807
NBM
_2NB
M_1
NBM
_3NB
M_5
NBM
_415
644
1768
718
806
1663
718
037
1733
319
369
1594
014
687
1458
517
770
1939
014
497
1837
416
408
1655
418
555
1618
916
304
1777
118
749
1483
417
632
1731
118
106
1347
919
585
1625
516
369
1737
214
506
1889
614
715
1905
715
838
1688
815
394
1574
617
973
1646
617
791
1439
617
567
1762
319
225
1624
915
934
1857
014
707
1919
418
763
1742
519
987
2066
515
948
1525
118
504
1804
517
065
1778
117
919
1903
914
397
1618
219
490
1559
915
256
1527
720
972
1530
320
869
1778
516
995
1837
317
570
1795
020
860
2086
118
105
1504
016
054
1780
120
800
1526
815
328
1813
115
465
1818
718
837
1904
717
224
1773
217
030
1580
020
607
1515
519
179
1517
516
998
2067
118
425
1525
015
819
1538
818
235
1542
414
677
1672
817
928
1749
717
814
1802
516
070
1707
620
164
1505
620
026
1912
815
036
2071
318
200
2062
318
191
2062
119
616
1969
414
437
1402
513
970
2080
618
310
1612
319
827
1512
215
747
2092
319
356
1451
219
621
1729
819
992
1496
915
835
1878
020
962
1953
719
528
1548
017
815
1644
613
895
1938
014
355
1649
216
677
1879
017
859
1951
516
308
1783
118
081
1642
717
758
2063
315
104
1827
120
655
1457
114
873
1876
120
058
1487
720
790
1622
314
443
1622
614
751
1938
620
228
2056
017
588
1738
819
993
1647
519
079
1908
019
274
1979
817
136
1918
217
385
1755
014
891
1849
215
492
2014
519
496
1673
814
026
1876
714
732
1751
117
268
1603
715
962
1455
619
778
2097
415
065
1774
219
319
1515
915
438
1917
214
278
2001
815
927
2059
920
180
1632
019
132
1753
516
596
1828
518
180
2011
916
276
1764
018
064
2000
517
665
1672
418
695
1405
520
780
1833
019
735
1505
115
448
1583
415
203
1434
820
573
1935
216
327
1895
115
330
1951
217
571
1526
718
457
1576
320
950
1554
418
298
1727
718
329
1691
618
871
1844
819
750
1802
618
948
1981
415
453
1956
116
819
1650
919
265
1913
419
511
1857
317
374
1952
516
579
1745
615
571
1873
618
889
1449
020
198
1765
818
407
1576
6
MSI2 text
IdSOX12STON1GPR150B3GNT7SIX4COL5A3ALPISLC32A1EPHA2PTPRUALPPL2ALDH1L1SGSM1ALPPSDC1PKD1L2SHISA2NAT8LCD300LDCKMACOT7CDKN2APHLDA3EPS8L1RCOR2KIAA1211LCSN2CXCR1MTUS2SMOC1C10orf71CEACAM5SERPINA11PSG6PSG5PSG9PSG8CLIC5CEACAM7CEACAM8SLC28A2IL36GSIGLEC8PPP1R9AFRZBCDC42BPACEACAM1SCML4KLK1RBP4FEZ1C3FFAR2CASP5CD48MRVI1CXCR2LTFDACT3PLXNA1DMWDBAI1PEAR1STAP2NLGN2LCN2EML5SYCP3MXD3SIGLEC12CNN3LAMC1FSTL1C14orf37TGM2HSPA2PPICCLEC5AMREGMPOIKZF2TSPAN7GPRASP2TTC30BMYOM1TOM1L1PRICKLE1FNBP1LNPHP3ASPHMCTP1PHACTR2FYBSLC15A2DYNC2LI1SERINC3ITPR2KLHDC2KLHL6CASP4ARNTL2C9orf91HEBP2SLC40A1MSI2ABLIM1SORL1TP53INP1TFPIITM2APAQR8LYPLAL1HEBP1HDDC3PNPTRAIPNT5DC2GPR125USP11CEACAM21SH2D1BSLC9A9FCGR3AEPS8PKIBHNMTRNASE2RNASE3SLC1A4IL1R2CKAP4SIGLEC9SIGLEC7FCGRTBCL2A1TREM1CYBBC5AR1G0S2S100A8S100A9C19orf59IRF8LGMNPTK2SERPINF1NRBP2TP53INP2FRAT1FRAT2NDRG2KLF6CDKN1AKLF2STK32CVILLPLSCR3PRAM1CFPAPOBRRASGRP4CSF1RCD300LFPLEKHG45-SepENGREPIN1FADS2NOTCH1CLSTN3SPNS2IFITM3IFITM2S100A6IFITM1TCF7BIN1
MIDLOWHigh
MSI2 text
relative
row min row max
GSM
6036
78G
SM60
3720
GSM
6037
25G
SM60
3731
GSM
6037
82G
SM60
3818
GSM
6037
94G
SM60
3795
GSM
6037
92G
SM60
3801
GSM
6038
17G
SM60
3806
GSM
6036
35G
SM60
3819
GSM
6037
98G
SM60
3805
GSM
6038
03G
SM60
3800
GSM
6038
07G
SM60
3796
GSM
6038
14G
SM60
3808
GSM
6037
93G
SM60
3815
GSM
6038
04G
SM60
3802
GSM
6037
99G
SM60
3776
GSM
6036
79G
SM60
3705
GSM
6038
94G
SM60
3696
GSM
6039
00G
SM60
3677
GSM
6037
15G
SM60
3816
GSM
6037
34G
SM60
3733
GSM
6037
45G
SM60
3687
GSM
6039
57G
SM60
3944
GSM
6036
91G
SM60
3716
GSM
6038
88G
SM60
3710
GSM
6037
28G
SM60
3713
GSM
6037
29G
SM60
3768
GSM
6039
09G
SM60
3948
GSM
6039
20G
SM60
3694
GSM
6039
33G
SM60
3896
GSM
6038
98G
SM60
3912
GSM
6036
64G
SM60
3886
GSM
6038
95G
SM60
3934
GSM
6037
17G
SM60
3736
GSM
6037
52G
SM60
3774
GSM
6037
38G
SM60
3778
GSM
6037
14G
SM60
3698
GSM
6036
92G
SM60
3690
GSM
6037
70G
SM60
3751
GSM
6037
85G
SM60
3784
GSM
6037
86G
SM60
3788
GSM
6037
87G
SM60
3634
GSM
6038
09G
SM60
3813
GSM
6036
23G
SM60
3638
GSM
6037
73G
SM60
3860
GSM
6036
21G
SM60
3637
GSM
6036
26G
SM60
3775
GSM
6037
79G
SM60
3841
GSM
6036
53G
SM60
3628
GSM
6036
49G
SM60
3812
GSM
6038
30G
SM60
3797
GSM
6038
11G
SM60
3823
GSM
6038
21G
SM60
3631
GSM
6036
29G
SM60
3767
GSM
6037
83G
SM60
3777
GSM
6038
10G
SM60
3824
GSM
6036
57G
SM60
3844
GSM
6036
51G
SM60
3674
GSM
6036
40G
SM60
3639
GSM
6036
27G
SM60
3645
GSM
6036
83G
SM60
3641
GSM
6036
22G
SM60
3835
GSM
6036
25G
SM60
3630
GSM
6038
33G
SM60
3826
GSM
6039
64G
SM60
3836
GSM
6038
65G
SM60
3943
GSM
6038
67G
SM60
3643
GSM
6038
37G
SM60
3861
GSM
6036
65G
SM60
3935
GSM
6039
47G
SM60
3941
GSM
6037
66G
SM60
3958
GSM
6039
40G
SM60
3938
GSM
6038
53G
SM60
3919
GSM
6039
65G
SM60
3863
GSM
6036
58G
SM60
3659
GSM
6036
61G
SM60
3669
GSM
6036
63G
SM60
3742
GSM
6037
26G
SM60
3689
GSM
6037
54G
SM60
3719
GSM
6037
57G
SM60
3847
GSM
6039
15G
SM60
3842
GSM
6039
03G
SM60
3932
GSM
6036
84G
SM60
3916
GSM
6039
01G
SM60
3755
GSM
6038
91G
SM60
3906
GSM
6039
08G
SM60
3675
GSM
6039
59G
SM60
3652
GSM
6038
32G
SM60
3732
GSM
6037
65G
SM60
3680
GSM
6036
62G
SM60
3744
GSM
6036
55G
SM60
3743
GSM
6037
35G
SM60
3647
GSM
6037
21G
SM60
3644
GSM
6037
50G
SM60
3700
GSM
6037
58G
SM60
3869
GSM
6038
99G
SM60
3962
GSM
6038
38G
SM60
3911
GSM
6039
52G
SM60
3646
GSM
6039
53G
SM60
3913
GSM
6039
71G
SM60
3904
GSM
6038
50G
SM60
3897
GSM
6037
37G
SM60
3708
GSM
6039
23G
SM60
3893
GSM
6037
07G
SM60
3704
GSM
6039
26G
SM60
3668
GSM
6038
71G
SM60
3877
GSM
6036
95G
SM60
3960
GSM
6038
72G
SM60
3749
GSM
6038
87G
SM60
3854
GSM
6036
70G
SM60
3939
GSM
6038
70G
SM60
3931
GSM
6038
75G
SM60
3881
GSM
6039
81G
SM60
3970
GSM
6037
11G
SM60
3693
GSM
6039
78G
SM60
3975
GSM
6036
97G
SM60
3633
GSM
6036
60G
SM60
3825
GSM
6038
58G
SM60
3790
GSM
6036
56G
SM60
3831
GSM
6036
50G
SM60
3820
GSM
6038
62G
SM60
3654
GSM
6036
48G
SM60
3843
GSM
6036
36G
SM60
3632
GSM
6037
64G
SM60
3739
GSM
6036
85G
SM60
3921
GSM
6037
91G
SM60
3706
GSM
6037
09G
SM60
3762
GSM
6037
60G
SM60
3740
GSM
6037
69G
SM60
3781
GSM
6037
71G
SM60
3882
GSM
6039
56G
SM60
3845
GSM
6039
14G
SM60
3983
GSM
6037
72G
SM60
3763
GSM
6039
61G
SM60
3946
GSM
6037
46G
SM60
3928
GSM
6036
86G
SM60
3703
GSM
6039
55G
SM60
3907
GSM
6038
89G
SM60
3942
GSM
6037
18G
SM60
3855
GSM
6038
64G
SM60
3857
GSM
6036
24G
SM60
3642
GSM
6038
90G
SM60
3937
GSM
6037
24G
SM60
3980
GSM
6038
49G
SM60
3688
GSM
6039
05G
SM60
3671
GSM
6037
41G
SM60
3918
GSM
6036
76G
SM60
3829
GSM
6037
23G
SM60
3977
GSM
6037
53G
SM60
3846
GSM
6039
22G
SM60
3868
GSM
6039
17G
SM60
3673
GSM
6036
99G
SM60
3780
GSM
6038
22G
SM60
3730
GSM
6036
81G
SM60
3727
GSM
6039
36G
SM60
3930
GSM
6038
85G
SM60
3701
GSM
6038
78G
SM60
3756
GSM
6038
73G
SM60
3759
GSM
6038
40G
SM60
3748
GSM
6038
51G
SM60
3747
GSM
6039
66G
SM60
3848
GSM
6038
56G
SM60
3892
GSM
6038
83G
SM60
3963
GSM
6039
25G
SM60
3969
GSM
6039
51G
SM60
3979
GSM
6039
76G
SM60
3879
GSM
6039
74G
SM60
3929
GSM
6039
67G
SM60
3666
GSM
6039
73G
SM60
3968
GSM
6038
74G
SM60
3982
GSM
6039
49G
SM60
3945
GSM
6039
72G
SM60
3880
GSM
6038
76G
SM60
3950
GSM
6038
84G
SM60
3852
GSM
6037
22G
SM60
3789
GSM
6037
12G
SM60
3902
GSM
6036
67G
SM60
3827
GSM
6038
66G
SM60
3839
GSM
6038
34G
SM60
3702
GSM
6036
72G
SM60
3682
GSM
6039
10G
SM60
3828
GSM
6039
27G
SM60
3924
GSM
6037
61G
SM60
3859
GSM
6039
54
1398
614
742
1855
117
745
1411
115
827
1834
518
171
1846
616
665
1775
418
211
1570
517
228
1903
417
739
1434
316
307
1551
717
974
1741
017
270
1885
518
339
1886
515
429
1394
615
038
1625
320
207
1543
419
211
1769
218
985
1808
617
681
1868
916
772
1924
414
239
1578
015
923
1742
414
559
1821
519
334
1675
819
762
1509
417
389
1960
316
745
1431
420
156
1576
517
197
1934
117
924
1896
815
025
1911
517
384
1943
020
025
1942
915
334
2088
914
787
1977
920
062
2068
515
568
1532
919
807
NBM
_2NB
M_1
NBM
_3NB
M_5
NBM
_415
644
1768
718
806
1663
718
037
1733
319
369
1594
014
687
1458
517
770
1939
014
497
1837
416
408
1655
418
555
1618
916
304
1777
118
749
1483
417
632
1731
118
106
1347
919
585
1625
516
369
1737
214
506
1889
614
715
1905
715
838
1688
815
394
1574
617
973
1646
617
791
1439
617
567
1762
319
225
1624
915
934
1857
014
707
1919
418
763
1742
519
987
2066
515
948
1525
118
504
1804
517
065
1778
117
919
1903
914
397
1618
219
490
1559
915
256
1527
720
972
1530
320
869
1778
516
995
1837
317
570
1795
020
860
2086
118
105
1504
016
054
1780
120
800
1526
815
328
1813
115
465
1818
718
837
1904
717
224
1773
217
030
1580
020
607
1515
519
179
1517
516
998
2067
118
425
1525
015
819
1538
818
235
1542
414
677
1672
817
928
1749
717
814
1802
516
070
1707
620
164
1505
620
026
1912
815
036
2071
318
200
2062
318
191
2062
119
616
1969
414
437
1402
513
970
2080
618
310
1612
319
827
1512
215
747
2092
319
356
1451
219
621
1729
819
992
1496
915
835
1878
020
962
1953
719
528
1548
017
815
1644
613
895
1938
014
355
1649
216
677
1879
017
859
1951
516
308
1783
118
081
1642
717
758
2063
315
104
1827
120
655
1457
114
873
1876
120
058
1487
720
790
1622
314
443
1622
614
751
1938
620
228
2056
017
588
1738
819
993
1647
519
079
1908
019
274
1979
817
136
1918
217
385
1755
014
891
1849
215
492
2014
519
496
1673
814
026
1876
714
732
1751
117
268
1603
715
962
1455
619
778
2097
415
065
1774
219
319
1515
915
438
1917
214
278
2001
815
927
2059
920
180
1632
019
132
1753
516
596
1828
518
180
2011
916
276
1764
018
064
2000
517
665
1672
418
695
1405
520
780
1833
019
735
1505
115
448
1583
415
203
1434
820
573
1935
216
327
1895
115
330
1951
217
571
1526
718
457
1576
320
950
1554
418
298
1727
718
329
1691
618
871
1844
819
750
1802
618
948
1981
415
453
1956
116
819
1650
919
265
1913
419
511
1857
317
374
1952
516
579
1745
615
571
1873
618
889
1449
020
198
1765
818
407
1576
6
MSI2 text
IdSOX12STON1GPR150B3GNT7SIX4COL5A3ALPISLC32A1EPHA2PTPRUALPPL2ALDH1L1SGSM1ALPPSDC1PKD1L2SHISA2NAT8LCD300LDCKMACOT7CDKN2APHLDA3EPS8L1RCOR2KIAA1211LCSN2CXCR1MTUS2SMOC1C10orf71CEACAM5SERPINA11PSG6PSG5PSG9PSG8CLIC5CEACAM7CEACAM8SLC28A2IL36GSIGLEC8PPP1R9AFRZBCDC42BPACEACAM1SCML4KLK1RBP4FEZ1C3FFAR2CASP5CD48MRVI1CXCR2LTFDACT3PLXNA1DMWDBAI1PEAR1STAP2NLGN2LCN2EML5SYCP3MXD3SIGLEC12CNN3LAMC1FSTL1C14orf37TGM2HSPA2PPICCLEC5AMREGMPOIKZF2TSPAN7GPRASP2TTC30BMYOM1TOM1L1PRICKLE1FNBP1LNPHP3ASPHMCTP1PHACTR2FYBSLC15A2DYNC2LI1SERINC3ITPR2KLHDC2KLHL6CASP4ARNTL2C9orf91HEBP2SLC40A1MSI2ABLIM1SORL1TP53INP1TFPIITM2APAQR8LYPLAL1HEBP1HDDC3PNPTRAIPNT5DC2GPR125USP11CEACAM21SH2D1BSLC9A9FCGR3AEPS8PKIBHNMTRNASE2RNASE3SLC1A4IL1R2CKAP4SIGLEC9SIGLEC7FCGRTBCL2A1TREM1CYBBC5AR1G0S2S100A8S100A9C19orf59IRF8LGMNPTK2SERPINF1NRBP2TP53INP2FRAT1FRAT2NDRG2KLF6CDKN1AKLF2STK32CVILLPLSCR3PRAM1CFPAPOBRRASGRP4CSF1RCD300LFPLEKHG45-SepENGREPIN1FADS2NOTCH1CLSTN3SPNS2IFITM3IFITM2S100A6IFITM1TCF7BIN1
MIDLOWHigh
MSI2 text
relative
row min row max
GS
M6
03
67
8G
SM
60
37
20
GS
M6
03
72
5G
SM
60
37
31
GS
M6
03
78
2G
SM
60
38
18
GS
M6
03
79
4G
SM
60
37
95
GS
M6
03
79
2G
SM
60
38
01
GS
M6
03
81
7G
SM
60
38
06
GS
M6
03
63
5G
SM
60
38
19
GS
M6
03
79
8G
SM
60
38
05
GS
M6
03
80
3G
SM
60
38
00
GS
M6
03
80
7G
SM
60
37
96
GS
M6
03
81
4G
SM
60
38
08
GS
M6
03
79
3G
SM
60
38
15
GS
M6
03
80
4G
SM
60
38
02
GS
M6
03
79
9G
SM
60
37
76
GS
M6
03
67
9G
SM
60
37
05
GS
M6
03
89
4G
SM
60
36
96
GS
M6
03
90
0G
SM
60
36
77
GS
M6
03
71
5G
SM
60
38
16
GS
M6
03
73
4G
SM
60
37
33
GS
M6
03
74
5G
SM
60
36
87
GS
M6
03
95
7G
SM
60
39
44
GS
M6
03
69
1G
SM
60
37
16
GS
M6
03
88
8G
SM
60
37
10
GS
M6
03
72
8G
SM
60
37
13
GS
M6
03
72
9G
SM
60
37
68
GS
M6
03
90
9G
SM
60
39
48
GS
M6
03
92
0G
SM
60
36
94
GS
M6
03
93
3G
SM
60
38
96
GS
M6
03
89
8G
SM
60
39
12
GS
M6
03
66
4G
SM
60
38
86
GS
M6
03
89
5G
SM
60
39
34
GS
M6
03
71
7G
SM
60
37
36
GS
M6
03
75
2G
SM
60
37
74
GS
M6
03
73
8G
SM
60
37
78
GS
M6
03
71
4G
SM
60
36
98
GS
M6
03
69
2G
SM
60
36
90
GS
M6
03
77
0G
SM
60
37
51
GS
M6
03
78
5G
SM
60
37
84
GS
M6
03
78
6G
SM
60
37
88
GS
M6
03
78
7G
SM
60
36
34
GS
M6
03
80
9G
SM
60
38
13
GS
M6
03
62
3G
SM
60
36
38
GS
M6
03
77
3G
SM
60
38
60
GS
M6
03
62
1G
SM
60
36
37
GS
M6
03
62
6G
SM
60
37
75
GS
M6
03
77
9G
SM
60
38
41
GS
M6
03
65
3G
SM
60
36
28
GS
M6
03
64
9G
SM
60
38
12
GS
M6
03
83
0G
SM
60
37
97
GS
M6
03
81
1G
SM
60
38
23
GS
M6
03
82
1G
SM
60
36
31
GS
M6
03
62
9G
SM
60
37
67
GS
M6
03
78
3G
SM
60
37
77
GS
M6
03
81
0G
SM
60
38
24
GS
M6
03
65
7G
SM
60
38
44
GS
M6
03
65
1G
SM
60
36
74
GS
M6
03
64
0G
SM
60
36
39
GS
M6
03
62
7G
SM
60
36
45
GS
M6
03
68
3G
SM
60
36
41
GS
M6
03
62
2G
SM
60
38
35
GS
M6
03
62
5G
SM
60
36
30
GS
M6
03
83
3G
SM
60
38
26
GS
M6
03
96
4G
SM
60
38
36
GS
M6
03
86
5G
SM
60
39
43
GS
M6
03
86
7G
SM
60
36
43
GS
M6
03
83
7G
SM
60
38
61
GS
M6
03
66
5G
SM
60
39
35
GS
M6
03
94
7G
SM
60
39
41
GS
M6
03
76
6G
SM
60
39
58
GS
M6
03
94
0G
SM
60
39
38
GS
M6
03
85
3G
SM
60
39
19
GS
M6
03
96
5G
SM
60
38
63
GS
M6
03
65
8G
SM
60
36
59
GS
M6
03
66
1G
SM
60
36
69
GS
M6
03
66
3G
SM
60
37
42
GS
M6
03
72
6G
SM
60
36
89
GS
M6
03
75
4G
SM
60
37
19
GS
M6
03
75
7G
SM
60
38
47
GS
M6
03
91
5G
SM
60
38
42
GS
M6
03
90
3G
SM
60
39
32
GS
M6
03
68
4G
SM
60
39
16
GS
M6
03
90
1G
SM
60
37
55
GS
M6
03
89
1G
SM
60
39
06
GS
M6
03
90
8G
SM
60
36
75
GS
M6
03
95
9G
SM
60
36
52
GS
M6
03
83
2G
SM
60
37
32
GS
M6
03
76
5G
SM
60
36
80
GS
M6
03
66
2G
SM
60
37
44
GS
M6
03
65
5G
SM
60
37
43
GS
M6
03
73
5G
SM
60
36
47
GS
M6
03
72
1G
SM
60
36
44
GS
M6
03
75
0G
SM
60
37
00
GS
M6
03
75
8G
SM
60
38
69
GS
M6
03
89
9G
SM
60
39
62
GS
M6
03
83
8G
SM
60
39
11
GS
M6
03
95
2G
SM
60
36
46
GS
M6
03
95
3G
SM
60
39
13
GS
M6
03
97
1G
SM
60
39
04
GS
M6
03
85
0G
SM
60
38
97
GS
M6
03
73
7G
SM
60
37
08
GS
M6
03
92
3G
SM
60
38
93
GS
M6
03
70
7G
SM
60
37
04
GS
M6
03
92
6G
SM
60
36
68
GS
M6
03
87
1G
SM
60
38
77
GS
M6
03
69
5G
SM
60
39
60
GS
M6
03
87
2G
SM
60
37
49
GS
M6
03
88
7G
SM
60
38
54
GS
M6
03
67
0G
SM
60
39
39
GS
M6
03
87
0G
SM
60
39
31
GS
M6
03
87
5G
SM
60
38
81
GS
M6
03
98
1G
SM
60
39
70
GS
M6
03
71
1G
SM
60
36
93
GS
M6
03
97
8G
SM
60
39
75
GS
M6
03
69
7G
SM
60
36
33
GS
M6
03
66
0G
SM
60
38
25
GS
M6
03
85
8G
SM
60
37
90
GS
M6
03
65
6G
SM
60
38
31
GS
M6
03
65
0G
SM
60
38
20
GS
M6
03
86
2G
SM
60
36
54
GS
M6
03
64
8G
SM
60
38
43
GS
M6
03
63
6G
SM
60
36
32
GS
M6
03
76
4G
SM
60
37
39
GS
M6
03
68
5G
SM
60
39
21
GS
M6
03
79
1G
SM
60
37
06
GS
M6
03
70
9G
SM
60
37
62
GS
M6
03
76
0G
SM
60
37
40
GS
M6
03
76
9G
SM
60
37
81
GS
M6
03
77
1G
SM
60
38
82
GS
M6
03
95
6G
SM
60
38
45
GS
M6
03
91
4G
SM
60
39
83
GS
M6
03
77
2G
SM
60
37
63
GS
M6
03
96
1G
SM
60
39
46
GS
M6
03
74
6G
SM
60
39
28
GS
M6
03
68
6G
SM
60
37
03
GS
M6
03
95
5G
SM
60
39
07
GS
M6
03
88
9G
SM
60
39
42
GS
M6
03
71
8G
SM
60
38
55
GS
M6
03
86
4G
SM
60
38
57
GS
M6
03
62
4G
SM
60
36
42
GS
M6
03
89
0G
SM
60
39
37
GS
M6
03
72
4G
SM
60
39
80
GS
M6
03
84
9G
SM
60
36
88
GS
M6
03
90
5G
SM
60
36
71
GS
M6
03
74
1G
SM
60
39
18
GS
M6
03
67
6G
SM
60
38
29
GS
M6
03
72
3G
SM
60
39
77
GS
M6
03
75
3G
SM
60
38
46
GS
M6
03
92
2G
SM
60
38
68
GS
M6
03
91
7G
SM
60
36
73
GS
M6
03
69
9G
SM
60
37
80
GS
M6
03
82
2G
SM
60
37
30
GS
M6
03
68
1G
SM
60
37
27
GS
M6
03
93
6G
SM
60
39
30
GS
M6
03
88
5G
SM
60
37
01
GS
M6
03
87
8G
SM
60
37
56
GS
M6
03
87
3G
SM
60
37
59
GS
M6
03
84
0G
SM
60
37
48
GS
M6
03
85
1G
SM
60
37
47
GS
M6
03
96
6G
SM
60
38
48
GS
M6
03
85
6G
SM
60
38
92
GS
M6
03
88
3G
SM
60
39
63
GS
M6
03
92
5G
SM
60
39
69
GS
M6
03
95
1G
SM
60
39
79
GS
M6
03
97
6G
SM
60
38
79
GS
M6
03
97
4G
SM
60
39
29
GS
M6
03
96
7G
SM
60
36
66
GS
M6
03
97
3G
SM
60
39
68
GS
M6
03
87
4G
SM
60
39
82
GS
M6
03
94
9G
SM
60
39
45
GS
M6
03
97
2G
SM
60
38
80
GS
M6
03
87
6G
SM
60
39
50
GS
M6
03
88
4G
SM
60
38
52
GS
M6
03
72
2G
SM
60
37
89
GS
M6
03
71
2G
SM
60
39
02
GS
M6
03
66
7G
SM
60
38
27
GS
M6
03
86
6G
SM
60
38
39
GS
M6
03
83
4G
SM
60
37
02
GS
M6
03
67
2G
SM
60
36
82
GS
M6
03
91
0G
SM
60
38
28
GS
M6
03
92
7G
SM
60
39
24
GS
M6
03
76
1G
SM
60
38
59
GS
M6
03
95
4
13
98
61
47
42
18
55
11
77
45
14
11
11
58
27
18
34
51
81
71
18
46
61
66
65
17
75
41
82
11
15
70
51
72
28
19
03
41
77
39
14
34
31
63
07
15
51
71
79
74
17
41
01
72
70
18
85
51
83
39
18
86
51
54
29
13
94
61
50
38
16
25
32
02
07
15
43
41
92
11
17
69
21
89
85
18
08
61
76
81
18
68
91
67
72
19
24
41
42
39
15
78
01
59
23
17
42
41
45
59
18
21
51
93
34
16
75
81
97
62
15
09
41
73
89
19
60
31
67
45
14
31
42
01
56
15
76
51
71
97
19
34
11
79
24
18
96
81
50
25
19
11
51
73
84
19
43
02
00
25
19
42
91
53
34
20
88
91
47
87
19
77
92
00
62
20
68
51
55
68
15
32
91
98
07
NB
M_2
NB
M_1
NB
M_3
NB
M_5
NB
M_4
15
64
41
76
87
18
80
61
66
37
18
03
71
73
33
19
36
91
59
40
14
68
71
45
85
17
77
01
93
90
14
49
71
83
74
16
40
81
65
54
18
55
51
61
89
16
30
41
77
71
18
74
91
48
34
17
63
21
73
11
18
10
61
34
79
19
58
51
62
55
16
36
91
73
72
14
50
61
88
96
14
71
51
90
57
15
83
81
68
88
15
39
41
57
46
17
97
31
64
66
17
79
11
43
96
17
56
71
76
23
19
22
51
62
49
15
93
41
85
70
14
70
71
91
94
18
76
31
74
25
19
98
72
06
65
15
94
81
52
51
18
50
41
80
45
17
06
51
77
81
17
91
91
90
39
14
39
71
61
82
19
49
01
55
99
15
25
61
52
77
20
97
21
53
03
20
86
91
77
85
16
99
51
83
73
17
57
01
79
50
20
86
02
08
61
18
10
51
50
40
16
05
41
78
01
20
80
01
52
68
15
32
81
81
31
15
46
51
81
87
18
83
71
90
47
17
22
41
77
32
17
03
01
58
00
20
60
71
51
55
19
17
91
51
75
16
99
82
06
71
18
42
51
52
50
15
81
91
53
88
18
23
51
54
24
14
67
71
67
28
17
92
81
74
97
17
81
41
80
25
16
07
01
70
76
20
16
41
50
56
20
02
61
91
28
15
03
62
07
13
18
20
02
06
23
18
19
12
06
21
19
61
61
96
94
14
43
71
40
25
13
97
02
08
06
18
31
01
61
23
19
82
71
51
22
15
74
72
09
23
19
35
61
45
12
19
62
11
72
98
19
99
21
49
69
15
83
51
87
80
20
96
21
95
37
19
52
81
54
80
17
81
51
64
46
13
89
51
93
80
14
35
51
64
92
16
67
71
87
90
17
85
91
95
15
16
30
81
78
31
18
08
11
64
27
17
75
82
06
33
15
10
41
82
71
20
65
51
45
71
14
87
31
87
61
20
05
81
48
77
20
79
01
62
23
14
44
31
62
26
14
75
11
93
86
20
22
82
05
60
17
58
81
73
88
19
99
31
64
75
19
07
91
90
80
19
27
41
97
98
17
13
61
91
82
17
38
51
75
50
14
89
11
84
92
15
49
22
01
45
19
49
61
67
38
14
02
61
87
67
14
73
21
75
11
17
26
81
60
37
15
96
21
45
56
19
77
82
09
74
15
06
51
77
42
19
31
91
51
59
15
43
81
91
72
14
27
82
00
18
15
92
72
05
99
20
18
01
63
20
19
13
21
75
35
16
59
61
82
85
18
18
02
01
19
16
27
61
76
40
18
06
42
00
05
17
66
51
67
24
18
69
51
40
55
20
78
01
83
30
19
73
51
50
51
15
44
81
58
34
15
20
31
43
48
20
57
31
93
52
16
32
71
89
51
15
33
01
95
12
17
57
11
52
67
18
45
71
57
63
20
95
01
55
44
18
29
81
72
77
18
32
91
69
16
18
87
11
84
48
19
75
01
80
26
18
94
81
98
14
15
45
31
95
61
16
81
91
65
09
19
26
51
91
34
19
51
11
85
73
17
37
41
95
25
16
57
91
74
56
15
57
11
87
36
18
88
91
44
90
20
19
81
76
58
18
40
71
57
66
MSI2 text
IdSOX12STON1GPR150B3GNT7SIX4COL5A3ALPISLC32A1EPHA2PTPRUALPPL2ALDH1L1SGSM1ALPPSDC1PKD1L2SHISA2NAT8LCD300LDCKMACOT7CDKN2APHLDA3EPS8L1RCOR2KIAA1211LCSN2CXCR1MTUS2SMOC1C10orf71CEACAM5SERPINA11PSG6PSG5PSG9PSG8CLIC5CEACAM7CEACAM8SLC28A2IL36GSIGLEC8PPP1R9AFRZBCDC42BPACEACAM1SCML4KLK1RBP4FEZ1C3FFAR2CASP5CD48MRVI1CXCR2LTFDACT3PLXNA1DMWDBAI1PEAR1STAP2NLGN2LCN2EML5SYCP3MXD3SIGLEC12CNN3LAMC1FSTL1C14orf37TGM2HSPA2PPICCLEC5AMREGMPOIKZF2TSPAN7GPRASP2TTC30BMYOM1TOM1L1PRICKLE1FNBP1LNPHP3ASPHMCTP1PHACTR2FYBSLC15A2DYNC2LI1SERINC3ITPR2KLHDC2KLHL6CASP4ARNTL2C9orf91HEBP2SLC40A1MSI2ABLIM1SORL1TP53INP1TFPIITM2APAQR8LYPLAL1HEBP1HDDC3PNPTRAIPNT5DC2GPR125USP11CEACAM21SH2D1BSLC9A9FCGR3AEPS8PKIBHNMTRNASE2RNASE3SLC1A4IL1R2CKAP4SIGLEC9SIGLEC7FCGRTBCL2A1TREM1CYBBC5AR1G0S2S100A8S100A9C19orf59IRF8LGMNPTK2SERPINF1NRBP2TP53INP2FRAT1FRAT2NDRG2KLF6CDKN1AKLF2STK32CVILLPLSCR3PRAM1CFPAPOBRRASGRP4CSF1RCD300LFPLEKHG45-SepENGREPIN1FADS2NOTCH1CLSTN3SPNS2IFITM3IFITM2S100A6IFITM1TCF7BIN1
MLL1 NPM1 FLT3 DNMT3A Clusters
Mutations:
MSI2 status
A
WTNAM
MLL
WTNAM
NPM1
WTNAM
FLT3
WTNAM
DNMT3A
WTNAM
splitMLL
WTTKDNA
FLT3_TKD
WTNAM
DNMT3A_other
987654A4321
cluster.id
WTNAM
MLLPTD
WTNAITD
FLT_ITD
WTNAM
DNMT3A_R882
MIDLOWHigh
MSI2.text
relative
row min row max
GS
M6
03
67
8G
SM
60
37
82
GS
M6
03
72
0G
SM
60
37
31
GS
M6
03
72
5G
SM
60
37
99
GS
M6
03
80
3G
SM
60
37
76
GS
M6
03
80
2G
SM
60
38
07
GS
M6
03
63
5G
SM
60
38
18
GS
M6
03
80
0G
SM
60
38
01
GS
M6
03
81
9G
SM
60
38
08
GS
M6
03
81
4G
SM
60
38
05
GS
M6
03
81
7G
SM
60
37
96
GS
M6
03
79
5G
SM
60
38
06
GS
M6
03
81
5G
SM
60
37
94
GS
M6
03
79
2G
SM
60
37
93
GS
M6
03
80
4G
SM
60
37
98
GS
M6
03
68
7G
SM
60
39
20
GS
M6
03
71
6G
SM
60
37
78
GS
M6
03
88
6G
SM
60
37
29
GS
M6
03
77
0G
SM
60
37
74
GS
M6
03
89
4G
SM
60
36
90
GS
M6
03
93
3G
SM
60
39
57
GS
M6
03
94
4G
SM
60
36
79
GS
M6
03
94
8G
SM
60
37
28
GS
M6
03
73
3G
SM
60
38
96
GS
M6
03
93
4G
SM
60
37
68
GS
M6
03
69
1G
SM
60
38
16
GS
M6
03
90
0G
SM
60
39
12
GS
M6
03
71
5G
SM
60
38
88
GS
M6
03
73
4G
SM
60
36
64
GS
M6
03
67
7G
SM
60
38
95
GS
M6
03
69
6G
SM
60
37
45
GS
M6
03
71
0G
SM
60
38
98
GS
M6
03
75
2G
SM
60
37
17
GS
M6
03
90
9G
SM
60
37
13
GS
M6
03
71
4G
SM
60
37
51
GS
M6
03
73
6G
SM
60
36
98
GS
M6
03
69
4G
SM
60
37
05
GS
M6
03
69
2G
SM
60
37
38
GS
M6
03
84
1G
SM
60
36
26
GS
M6
03
63
7G
SM
60
36
34
GS
M6
03
62
1G
SM
60
36
23
GS
M6
03
77
3G
SM
60
38
09
GS
M6
03
77
5G
SM
60
36
38
GS
M6
03
65
3G
SM
60
38
13
GS
M6
03
86
0G
SM
60
37
79
GS
M6
03
78
4G
SM
60
37
85
GS
M6
03
78
6G
SM
60
37
87
GS
M6
03
78
8G
SM
60
37
83
GS
M6
03
62
5G
SM
60
39
19
GS
M6
03
84
4G
SM
60
39
43
GS
M6
03
67
4G
SM
60
38
21
GS
M6
03
94
7G
SM
60
36
59
GS
M6
03
66
1G
SM
60
36
63
GS
M6
03
64
5G
SM
60
36
58
GS
M6
03
68
3G
SM
60
36
39
GS
M6
03
83
6G
SM
60
39
35
GS
M6
03
96
5G
SM
60
38
30
GS
M6
03
96
4G
SM
60
38
10
GS
M6
03
79
7G
SM
60
38
24
GS
M6
03
62
8G
SM
60
36
22
GS
M6
03
64
9G
SM
60
36
27
GS
M6
03
95
8G
SM
60
36
29
GS
M6
03
65
7G
SM
60
38
37
GS
M6
03
63
0G
SM
60
38
33
GS
M6
03
63
1G
SM
60
38
11
GS
M6
03
94
0G
SM
60
38
35
GS
M6
03
93
8G
SM
60
36
41
GS
M6
03
76
6G
SM
60
37
67
GS
M6
03
94
1G
SM
60
38
12
GS
M6
03
86
5G
SM
60
38
23
GS
M6
03
64
3G
SM
60
36
51
GS
M6
03
85
3G
SM
60
36
40
GS
M6
03
86
7G
SM
60
38
26
GS
M6
03
86
3G
SM
60
37
77
GS
M6
03
86
1G
SM
60
36
65
GS
M6
03
74
2G
SM
60
36
69
GS
M6
03
87
7G
SM
60
38
71
GS
M6
03
66
8G
SM
60
38
70
GS
M6
03
86
9G
SM
60
39
81
GS
M6
03
89
7G
SM
60
39
03
GS
M6
03
97
1G
SM
60
38
87
GS
M6
03
66
2G
SM
60
36
55
GS
M6
03
72
1G
SM
60
39
01
GS
M6
03
75
5G
SM
60
37
50
GS
M6
03
75
8G
SM
60
39
06
GS
M6
03
69
7G
SM
60
38
54
GS
M6
03
76
5G
SM
60
36
44
GS
M6
03
97
0G
SM
60
39
32
GS
M6
03
64
6G
SM
60
38
72
GS
M6
03
89
9G
SM
60
36
89
GS
M6
03
74
3G
SM
60
37
32
GS
M6
03
95
3G
SM
60
36
52
GS
M6
03
87
5G
SM
60
38
38
GS
M6
03
71
9G
SM
60
38
32
GS
M6
03
72
6G
SM
60
36
84
GS
M6
03
91
1G
SM
60
39
62
GS
M6
03
75
7G
SM
60
39
52
GS
M6
03
84
2G
SM
60
38
91
GS
M6
03
90
8G
SM
60
38
93
GS
M6
03
70
8G
SM
60
37
00
GS
M6
03
96
0G
SM
60
37
54
GS
M6
03
64
7G
SM
60
37
11
GS
M6
03
67
5G
SM
60
39
59
GS
M6
03
85
0G
SM
60
37
44
GS
M6
03
93
9G
SM
60
39
75
GS
M6
03
97
8G
SM
60
37
04
GS
M6
03
93
1G
SM
60
39
26
GS
M6
03
74
9G
SM
60
38
81
GS
M6
03
90
4G
SM
60
39
13
GS
M6
03
68
0G
SM
60
37
07
GS
M6
03
92
3G
SM
60
37
35
GS
M6
03
73
7G
SM
60
39
16
GS
M6
03
69
5G
SM
60
38
47
GS
M6
03
91
5G
SM
60
36
70
GS
M6
03
69
3G
SM
60
38
25
GS
M6
03
64
2G
SM
60
38
40
GS
M6
03
84
6G
SM
60
38
83
GS
M6
03
79
0G
SM
60
37
81
GS
M6
03
90
5G
SM
60
37
91
GS
M6
03
93
7G
SM
60
38
82
GS
M6
03
70
6G
SM
60
37
60
GS
M6
03
94
2G
SM
60
39
66
GS
M6
03
91
8G
SM
60
37
39
GS
M6
03
72
3G
SM
60
38
92
GS
M6
03
97
4G
SM
60
39
79
GS
M6
03
97
7G
SM
60
38
48
GS
M6
03
85
2G
SM
60
38
55
GS
M6
03
97
3G
SM
60
39
67
GS
M6
03
85
6G
SM
60
38
68
GS
M6
03
68
8G
SM
60
38
49
GS
M6
03
76
9G
SM
60
37
71
GS
M6
03
93
0G
SM
60
36
54
GS
M6
03
69
9G
SM
60
39
69
GS
M6
03
63
6G
SM
60
36
60
GS
M6
03
96
1G
SM
60
36
56
GS
M6
03
73
0G
SM
60
38
31
GS
M6
03
87
3G
SM
60
36
24
GS
M6
03
94
9G
SM
60
37
03
GS
M6
03
98
0G
SM
60
38
74
GS
M6
03
90
7G
SM
60
37
72
GS
M6
03
72
4G
SM
60
38
22
GS
M6
03
88
9G
SM
60
38
79
GS
M6
03
98
3G
SM
60
38
85
GS
M6
03
75
6G
SM
60
36
76
GS
M6
03
63
2G
SM
60
36
33
GS
M6
03
64
8G
SM
60
38
20
GS
M6
03
87
6G
SM
60
37
01
GS
M6
03
84
3G
SM
60
37
27
GS
M6
03
68
5G
SM
60
36
81
GS
M6
03
96
8G
SM
60
39
82
GS
M6
03
85
1G
SM
60
39
72
GS
M6
03
92
9G
SM
60
37
18
GS
M6
03
75
9G
SM
60
37
09
GS
M6
03
89
0G
SM
60
36
50
GS
M6
03
94
5G
SM
60
39
50
GS
M6
03
95
1G
SM
60
39
46
GS
M6
03
74
6G
SM
60
37
80
GS
M6
03
75
3G
SM
60
39
55
GS
M6
03
92
8G
SM
60
38
29
GS
M6
03
92
5G
SM
60
38
58
GS
M6
03
95
6G
SM
60
38
57
GS
M6
03
97
6G
SM
60
38
62
GS
M6
03
74
7G
SM
60
38
80
GS
M6
03
67
1G
SM
60
36
86
GS
M6
03
88
4G
SM
60
37
63
GS
M6
03
87
8G
SM
60
39
22
GS
M6
03
76
2G
SM
60
39
36
GS
M6
03
86
4G
SM
60
36
73
GS
M6
03
84
5G
SM
60
39
14
GS
M6
03
96
3G
SM
60
39
17
GS
M6
03
76
4G
SM
60
39
21
GS
M6
03
74
8G
SM
60
37
40
GS
M6
03
66
6G
SM
60
37
41
GS
M6
03
68
2G
SM
60
37
12
GS
M6
03
70
2G
SM
60
37
89
GS
M6
03
83
9G
SM
60
36
72
GS
M6
03
86
6G
SM
60
36
67
GS
M6
03
90
2G
SM
60
38
27
GS
M6
03
83
4G
SM
60
37
22
GS
M6
03
92
7G
SM
60
39
54
GS
M6
03
76
1G
SM
60
38
59
GS
M6
03
91
0G
SM
60
38
28
GS
M6
03
92
4
MSI2.textcluster.idDNMT3A
DNMT3A_R882DNMT3A_other
FLT3FLT_ITD
FLT3_TKDNPM1
MLLPTDsplitMLL
MLL
IdSOX12STON1GPR150COL5A3SLC32A1EPHA2ALPIALPPL2PTPRUSIX4SGSM1B3GNT7ALDH1L1ALPPSDC1PKD1L2SHISA2NAT8LACOT7CD300LDCKMCDKN2APHLDA3EPS8L1RCOR2KIAA1211LCSN2CXCR1MTUS2SMOC1C10orf71CEACAM5SERPINA11PSG6PSG5PSG9PSG8CLIC5CEACAM7CEACAM8SLC28A2SIGLEC8IL36GCEACAM1KLK1RBP4FEZ1SCML4EML5LCN2PPP1R9AFRZBCDC42BPACASP5CD48MRVI1CXCR2DACT3PLXNA1DMWDBAI1PEAR1STAP2NLGN2LTFMXD3SIGLEC12SYCP3PPICTGM2HSPA2CNN3LAMC1FSTL1C14orf37GPRASP2TTC30BMPOCLEC5AMREGIKZF2TSPAN7FNBP1LNPHP3ASPHMCTP1FYBSLC15A2PHACTR2ARNTL2DYNC2LI1SERINC3ITPR2KLHDC2SLC40A1KLHL6C9orf91HEBP2CASP4HEBP1LYPLAL1MSI2ABLIM1SORL1TP53INP1TFPIITM2AGPR125MYOM1TOM1L1PRICKLE1PAQR8HNMTSH2D1BSLC9A9FCGR3AHDDC3TRAIPNT5DC2CEACAM21EPS8PKIBC3FFAR2SIGLEC9SIGLEC7FCGRTBCL2A1TREM1CYBBC5AR1G0S2S100A8S100A9C19orf59CKAP4IRF8IL1R2RNASE2RNASE3SLC1A4PNPFRAT1FRAT2LGMNPTK2SERPINF1NDRG2NRBP2TP53INP2KLF6STK32CCDKN1AKLF2VILLPLSCR3PRAM1CFPAPOBRCSF1RRASGRP4CD300LFENGUSP11REPIN1FADS2NOTCH1CLSTN3SPNS2PLEKHG45-SepIFITM3IFITM2S100A6IFITM1TCF7BIN1
WTNAM
MLL
WTNAM
NPM1
WTNAM
FLT3
WTNAM
DNMT3A
WTNAM
splitMLL
WTTKDNA
FLT3_TKD
WTNAM
DNMT3A_other
987654A4321
cluster.id
WTNAM
MLLPTD
WTNAITD
FLT_ITD
WTNAM
DNMT3A_R882
MIDLOWHigh
MSI2.text
relative
row min row max
GS
M6
03
67
8G
SM
60
37
82
GS
M6
03
72
0G
SM
60
37
31
GS
M6
03
72
5G
SM
60
37
99
GS
M6
03
80
3G
SM
60
37
76
GS
M6
03
80
2G
SM
60
38
07
GS
M6
03
63
5G
SM
60
38
18
GS
M6
03
80
0G
SM
60
38
01
GS
M6
03
81
9G
SM
60
38
08
GS
M6
03
81
4G
SM
60
38
05
GS
M6
03
81
7G
SM
60
37
96
GS
M6
03
79
5G
SM
60
38
06
GS
M6
03
81
5G
SM
60
37
94
GS
M6
03
79
2G
SM
60
37
93
GS
M6
03
80
4G
SM
60
37
98
GS
M6
03
68
7G
SM
60
39
20
GS
M6
03
71
6G
SM
60
37
78
GS
M6
03
88
6G
SM
60
37
29
GS
M6
03
77
0G
SM
60
37
74
GS
M6
03
89
4G
SM
60
36
90
GS
M6
03
93
3G
SM
60
39
57
GS
M6
03
94
4G
SM
60
36
79
GS
M6
03
94
8G
SM
60
37
28
GS
M6
03
73
3G
SM
60
38
96
GS
M6
03
93
4G
SM
60
37
68
GS
M6
03
69
1G
SM
60
38
16
GS
M6
03
90
0G
SM
60
39
12
GS
M6
03
71
5G
SM
60
38
88
GS
M6
03
73
4G
SM
60
36
64
GS
M6
03
67
7G
SM
60
38
95
GS
M6
03
69
6G
SM
60
37
45
GS
M6
03
71
0G
SM
60
38
98
GS
M6
03
75
2G
SM
60
37
17
GS
M6
03
90
9G
SM
60
37
13
GS
M6
03
71
4G
SM
60
37
51
GS
M6
03
73
6G
SM
60
36
98
GS
M6
03
69
4G
SM
60
37
05
GS
M6
03
69
2G
SM
60
37
38
GS
M6
03
84
1G
SM
60
36
26
GS
M6
03
63
7G
SM
60
36
34
GS
M6
03
62
1G
SM
60
36
23
GS
M6
03
77
3G
SM
60
38
09
GS
M6
03
77
5G
SM
60
36
38
GS
M6
03
65
3G
SM
60
38
13
GS
M6
03
86
0G
SM
60
37
79
GS
M6
03
78
4G
SM
60
37
85
GS
M6
03
78
6G
SM
60
37
87
GS
M6
03
78
8G
SM
60
37
83
GS
M6
03
62
5G
SM
60
39
19
GS
M6
03
84
4G
SM
60
39
43
GS
M6
03
67
4G
SM
60
38
21
GS
M6
03
94
7G
SM
60
36
59
GS
M6
03
66
1G
SM
60
36
63
GS
M6
03
64
5G
SM
60
36
58
GS
M6
03
68
3G
SM
60
