564 K u r z e Or ig ina lmi t t e i l ungen Die Natur- wissenschaften

the initial PCO~ in the blood a n d also the initial pulmonary vascular resistance. Role of s u p r a sp ina l nel l raxis a n d also tile a u t o n o m i c n e r v o u s s y s t e m in ref lex m a n i f e s t a t i o n s of t he se r e sp i ra to ry blood p ressu re waves are u n d e r s tudy .

Department o/ Physiology, 92, Acharya Pra/ulla Chandra Road, Calcutta-9, India S.R., M&ITRA, S. ])AS*), S. CHATTERJEE**) and ]3. N. KOLEY

Eingegangen am t0. August ~965

*) IRes. Assistant. **) R.A.0., I C.M.R. a) DUBOls, A.B.: J. Appl. Physiol. s, ~ (1952). -- 2)BlSCOE,

T .J . , and M.J. PURVES: J. Physiol. (London) 177, 72p (1965). -- ~) DIRKEd, M.N.J . , and H. I-IEEMSTRA: Quart. J. Exptl. PhysioI. 34, 193 (1948). -- 4) DUKB, H.N. : Quart. J. Exptl. Physiol. 3s, 25 (1949); 36, 75 (195t). -- ~) NISELL, O.: Acta Physiol. Seand. 23, 85 (195~). -- ~) PETER, W., ])'. PETER, SALISBURY, and S. DAVID: Am. J. Physiol. 191 (3), 453 (1957).

L'influence de la nialamide sur l'enc6phalomy61ite allergique exp4rimentale

N o u s avolls d4j~ m o n t r d qlle dans le ce rveau des cobayes a t te i l l t s d ' encdphMomydl i t e allergiqlle expdr imel l ta le (EAR) on c o n s t a t e u n e a u g m e n t a t i o n de ! 'act ivi td de la mo l loamine o x y d a s e (MAO), due p r o b a b l e m e n t k l ' in te l l s i f ica t ion du md tabo l i sme de la sdrotonine~),~ C 'es t pou r ce t te ra i son que ilOUS a v o n s essay6 d ' inf luel lcer l 'dvolntioI1 de I ' E A E pa r des inh ib i t eu r s de la m o n o a m i n e o x y d a s e (IMAO).

On a employd d e u x groupes de cobayes adu l t e s mascu l ins , pesa l l t 500 - -700 g, qu i o n t 4t4 inoculds avec lln h o m o g d n a t de ce rveau hdt6rologue, emul s ionnd d a n s l ' a d j o u v a n t comple t de Freul ld. Le p remie r groupe, c o m p r e n a n t douze a n i m a u x , a dtd injectd c h a q u e ]our, pa r vote i l l t rapdri tondale , avec 25 m g / k g de n i a l amide (Niamid Pfi tzer) . Le 5-6me ]our pa r t i r de la premi6re in ject ion, les a n i m a n x on t dt4 inoculds p a r voie i n t r ade rm i que , d a n s les pa t t e s , avec 0. t m l d 'dml l l s ion el lc@hali tog4niql le , 4 t a n t 5. la su i te in jectd j o u r n e l l e m e n t avec de la l l ia lamide, j u s q u ' a u t8-@me j o u r aprgs l ' i nocu la t ion encdphaI i togdnique . Le second g roupe (tdmoin), c o m p r e n a n t d ix a l l imaux , a 6t6 inocul6 n n e seule lois s e u l e m e n t avec l 'dmulsiol l encdphal i togdnique . Les a n i m a l l x on t dt6 4xamind c h a q n e jollr, a f in de su rp r end re l ' appa r i t i on des s y m p t o m e s de I 'EAE. Les a n i m a u x qu i on t pr4sellt4 des s y m p t o m e s dv identes d ' E A E (par6ses, para lys ies , convuls ions) ou t dr6 sacrifids le ]our du d6bu t de la maladie . Le t8 -hme ]our apr6s l ' i nocu la t ion an t igdnique , t o u s l e s a n i m a l l x s u r v i v a n t s des d e u x groupes o n t 6t6 sacrifi4s et le ce rveau a dt4 fix6 dalls la fo rmal ine p o u r les e x a m e n s h i s topa tho log iques .

D u g roupe t d m o i n (non inject6 avec la n ia lamide) , h n i t a l l imal lx ollt p rdsent6 des s y m p t o m e s d ' E A E , c inq le f t - 6 m e ]our et t ro is le t 2 -6me ]ours apr6s l ' i nocu la t ion de l '6mulsiol l enc@hal i tog6n ique . D u g roupe inject6 avec la n ia lamide , c inq a n i m a u x s o n t t o m b 4 s malades , q u a t r e le 12-6me ]our et ull le t 4-6me j our apr@s l ' i nocu la t ion an t ig4nique . Les a l l imaux qu i ne SOllt pa s t o m b d s m a l a d e s j u s q u ' a u t8 -6me ]our, n ' o n t pa s prdsent6 des ldsions d ' E A E a u x e x a m e n s h i s topa tho lo - g iques , t and i s qne les au t r e s ou t pr4sent6 des ldsions diffuses d ' E A E .

De ce t te expdrience, il en r4sul te que Ia n i a l amide a une ac t ion inhib i t r ice su r l 'appari t ioi1 de I 'EAE. Ainsi, du groupe t6moin , s o n t t o m b 6 s m a l a d e s 80 pou r cent , t and i s que du groupe illject6 avec la n i a l amide s e u l e m e n t 4t pou r cen t des a n i m a u x .

I1 es t ~ p r d s u m e r que la dose admin i s t r6e pa r nous , n ' a pas dr4 suff isal l te et que des doses p lus dlev4es peuve l l t s u p p r i m e r compl~ temel l t l ' appa r i t i on de I 'EAE. JaNI~ovld et coil. S) on t reIatd la suppres s ion de I ' E A E chez Ies r a t s t ra i t4s a v e c l a r4serpine, a t t r i b u a n t l 'e f fe t de cet te s u b s t a n c e k son ac t ion su r le t h y m u s . Nous es t imol ls que Fac t ion de Ia rgserpine dalls I ' E A E es t due ~ son effet su r la s4ro ton ine cdr6brale. A l 'dven- tua t i t6 que l ' ac t iv i t6 de la N2AO es t a n g m e n t d n o n s e u l e m e n t d a n s I 'EAE, ma t s auss i d a n s d ' a u t r e s cas de hypersens ib i l i td du t y p e r e t a r d s I ' IMAO p o u r r a i e n t in f luencer anss i ces derni~res r6act ions. L ' e m p l o i de I ' IMAO d a n s le t r a i t e m e n t des ma lad ie s d6my6I in i san tes et des ma lad ies d ' a u t o a g r e s s i o n chez l ' h o m m e sera i t a insi utile.

Insti t~t de Mddecine, D@artement de Physiopathologie, Buearest (Roumanir

M. SARAGEA et A. VLA-DUTIU

Eingegangen am 28. Juni 1965

I) SARAGEA, M., M. CLOPOTARU, N. ROTARU, T. NEGRU, A~[.GICX et A. VLADUTIU: National Conference of physiology, Bucharest,

7 oct. 1964 (abstracts), p. 184. __ 2)SARAOEA,.~VI., N. ROTARU, T. NEGRU et A. VLADUTIU: Nature 206, 306 (t965). - - a) JA~;KOVld, B.D., M. DRASKOCI, D. PAUNOVld et L. PoeEsKovld: Nature 204, t101 (t964).

Studies on the Pituitary Gland of the Mahseer, Barbus tog (Tor tor)

Tile h y p o p h y s i s of Barbus ~or is a s y m m e t r i c a l ovoid s t r u c t u r e s l igh t ly compressed in t h e dorso-ven t ra l plane. I t is a p p e n d e d to t h e i n fund ibu l a r region of t h e d iencepha lon by m e a n s of a delicate sho r t s t a lk j u s t beh ind t he opt ic ci l iasma. Tile m a i n b o d y of t h e g land pro jec ts ou t in to t h e m y o d o m e t h r o u g h fossa h y p o p h y s e o s . I t lies in all obl ique cral l io-caudM plane , w i th i ts b road end pos ter ior ly a n d t ape r i ng end an- teriorly. The b road end, topographica l ly , belongs to tile an te r ior side, a n d tile t ape r i ng end to ti le pos ter ior side. B u t t h e o r i en ta t ion of t i le p i t u i t a r y has been b r o u g h t a b o u t b y a ro t a t i on of t h e g land t h r o u g h an angle of 180 ~ du r ing devel- opmen t . T h e g l and is caudo- lep tobas ic type.

Mid-sag i t t a l sect ions display, a pa r s distalis, a pa r s in ter - med i a a n d a l l eurohypophys i s . The pa r s dis ta l is is, t inctor ia l ly , d i f fe ren t ia ted in to t he ros t ra l pa r s dis ta l is a n d t he p r o x i m a l pa r s distalis . DAWSON a n d FmEDGOOD'S t r i ch rome techn ique , fol lowing mercu r i c - fo rma ldehyde f ixat ion, d e m o n s t r a t e s t h a t t he ros t ra l pa r s dis ta l is is charac te r ized by t h e p reponde rance of azocarminophi l i c a n d orallgeophilic acidophi ls a n d b y t h e presence of on ly a few cyanopili ls . Tile cyanophi l s f o rm a consp icuous fea ture of t he p r o x i m a l pa r s dis tal is which also con ta ins orangeophi l ic acidopili ls a long wi th azocarmil lophi ls . I t is i n t e re s t ing to no te t h e presence of azocarminophi l ic in- c lusions in. t i le b lue c y t o p l a s m of t he cyanophi ls . Only t h e cyanoph i l s are s t a ined w i th P A S a n d A F techn iques , fol lowing m e r c u r i c - f o r m a l d e h y d e a n d Bou in ' s f ixat ion. Tile pa r s in ter- med i a con ta ins t h e cyanophi l s , ac idophi ls a n d ch romophobes . The cyal lophi ls t a k e dull h u e w i th ani l ine blue, while acido- phi ls a re s t a ined w i th o range G. The cyanophi l s are P A S a n d A F nega t ive , b u t t h e y are s t a ined w i th ph lox ine of c h r o m a l u m / h a e m a t o x y l i n fol lowing B o u i n ' s f ixa t ion .

T h e neu rosec re to ry f ibres a n d ma te r i a l s in t h e neuro- h y p o p h y s i s are s t a ined wi th A F a n d ci lrom a l u m / h a e m a t o x y l i n t echn iques . The re appea r s to ex is t an i n t i m a t e re la t ion be- tween t h e p a r e n c h y m a cells of t he pa r s i n t e rmed ia an d tile neu rosec re to ry mater ia l s . The neu rosec re to ry ma te r i a l s show f l uc tua t i on du r ing t he biological cycle of th i s species.

The cyal lophi ls of t h e p rox ima l pa r s dis tal is d i sp lay c o n c o m i t a n t changes w i th those occur r ing in t h e g o n ad s du r ing t h e biological cycle of th i s species. I-ITy-pertrophy, hyperp las ia , in t ense g ranu la t ion , vacuo la t i on and degranu la - t ion charac te r ize these cyanophi l s du r ing t h e s p a w i n g period. These cyal lophi ls are ident i f ied as gonado t rophs .

The a u t h o r is g ra te fu l to Prof. H. SwARIJP for sugges t i ng and supe rv i s ing th i s s tudy .

Department o/ Zoology, Th. 1). S. B. Government College, Nainital, India

B.P. RAI*) Eingegangen am 9. August 1965

*) Present address: School of Studies in Zoology, Vikram Uni- versity, Ujjain (India).

Kontriire pharmakodynamische Antwortweise der gutartigen und der kanzer~sen Epidermishyperplasie der RA-Miiuse

auf alas onkolytische Alkaloid der V i n c a rosea Linn.

Diese Arbe i t i s t ein Glied eines A r b e i t s p r o g r a m m s z u m Zweck der En twick lu l lg I leuart iger, se lekt iv w i rk samer krebs- c i l emo the rapeu t i s che r Mittel. Die Ar t der Al l twor tweise zweier pathologisc i ler Zust~Lnde, volt gegens~tz l icher Na tu r , eines g e m e i n s a m e n bomologen Ausgal lgsgewebes , der M~use- epidermis , d i en t als Erfolgsgewebe. Fr t ther i s t mi tge te i l t wor- den, dab Noxen , wie z .B . Na t r i umpen toba rb i t aV~) , Colcili- cin lb~ sowie ionis ierende St rahlungl~b) , ) e inersei ts au f die gu ta r t ige , andere rse i t s au f die kanzer6se Ep ide rmishype rp l a s i e der 2Claus in q u a n t i t a t i v u n d qua l i t a t i v v o n e i n a n d e r diver- g ie render Weise an twor t en .

Vor l iegend wird fiber dell E f f ek t eines ol lkolyt isch wirken- dell, cilemiscil d ime t r i s chen Alkaloids aus der Vines roses Linn . (Vinblas t i l l su lpha t : Velbe | El i Lil ly Co., I l ldiallapolis, U.S.A.) llacil 1-, 2- bzw. 3mal iger top ischer App l ika t ion auf die H a u t der Mans voll 85 t ' der Subs t anz ill Ace ton ber ichte t . Das Mater ia l u m f a B t 145 weibl iche Ni~iuse des hau tgesc i lwuls t - res is tente l l R A - S t a m m e s , 75000 Zell tei lullgsfigurell u n d die