Student: Jan ČesalLeader: Václav Šlouf

Effect of the Solvent Environment on Excited-State Properties of an Untypical

Carotenoid

27. 07. 2011

Motivation - earlier measurements on LH1-RC of AAP

(Aerobic Anoxygenic Phototrophic) bacteria

1

111

ETc

Bacteriorubixanthinal BacteriochlorophyllS0 S0

S2

S1

Qy

Qx

ET

Hu et al., PNAS 1998

c = 0.6 ps

ET = ?

1 = ... will be the result of this study

Materials and methodsPump–probe spectroscopy - 110 fs- 1 kHz

Pump

Crystal

Laser system

Delay line

Light converter

Sample

Probe

Spectrograph

Materials and methods 2

• Extraction of Bacteriorubixanthinal (Brx)- HPLC (High-performance liquid chromatography)

[min.]Time

0 10 20 30 40

[V]

Vol

tage

0,0

0,2

0,4

0,6

0,8

1,0

1,2

[%]

Com

ponents

0

20

40

60

80

2,8

1

3,2

2

3,9

3

5,5

4

7,0

5

8,0

6 8,

5

7

9,2

8

9,8

9

12,7

1

0

13,3

1

1

14,2

1

214

,7

13

15,5

1

4

17,0

1

5

18,0

1

6

19,5

1

7

22,4

1

8

23,3

1

9

24,2

2

0

25,4

2

1

27,1

2

227

,6

23

28,4

2

4

29,2

2

5

29,9

2

6

31,0

2

7

32,1

2

8

33,7

2

9

34,6

3

0

36,8

3

1

37,6

3

2

39,7

3

3

42,3

3

4

43,5

3

5

C:\ data_prep\ LCdata\Kata\BRX11-07-15-C - INT7 - 1

http://en.wikipedia.org/wiki/File:Agilent1200HPLC.jpg

Results

Steady-state spectroscopy

500nm

Transient absorption

spectra

Kinetics

ps

Conclusions

- We measured 1

- We calculated ET 1

111

ETc

Brx BChlS0 S0

S2

S1

Qy

Qx

= 3.4 ps

ET = 0.73 ps

Thanks for your attention