Sts conditioner, a product in development with large potential

STS ConditionerA Developmental Product from Sustainable Technology Systems

• STS Biomass Conditioner is a chemical solution that reduces into an environmentally safe product.

• STS Conditioner eliminates/ reduces the use of biofuels industrial antibiotics that are polluting our waterways

• STS Conditioner softens the lignin into liquid and/or gel depending on the feedstock thereby exposing more surface area for enzymatic action. This saves time and money in mashing and shredding biomass.

• STS Conditioner eliminates invasive bacteria that will compete for glucose after starch reduction. Glucose is the basic sugar that forms alcohol.

• STS Conditioner has proven to produce over 8.5 percent alcohol increase per batch (even 1% is significant to industry). Greater yields are anticipated after completing this study.

• STS Conditioner reduces the cost of enzymes which is one of the most costly chemical purchases for the biofuels producer.

STS ConditionerA Developmental Product from Sustainable Technology Systems

• STS Conditioner eliminates offensive odors. Cattail rhizomes are covered

with a bacterial biofilm which cannot be scrubbed off or killed with low

heat. Once the gross bioburden is removed (dirt or mud) STS conditioner

eliminates the biofilm.

• STS Conditioner is bacteriocidal and it promotes healthy yeast growth.

• STS Conditioner is significantly lower in price than Lactrol and Beta

Hops.

Cara Mullen, MPHSustainable Technology Systems108 W. 22ndHutchinson, KS 67502www.sustainabletechsys.comCell: 620-960-6114

Peggy KorthSustainable Technology Systems40 Sun Valley Dr. Spring Branch TX 78070 FAX 820 [email protected]

http://www.sustainabletechsys.com/

mailto:[email protected]

Studies of antibacterial effect of STS Conditioner with corn mash samples from corn ethanol plants.

• The goal was to find the lowest concentration of STS CONDITIONER that will kill bacteria but not impede the growth or fermentation efficiency of the yeast.

• We performed studies with the corn mash sample from Prairie Horizon. – The corn mash sample was taken prior to the prop so we needed to add

yeast. – Before adding anything we tested for growth and found two populations

that seem to be bacteria and a fungus. – The latter has a phenotype that is distinctly different from bacteria and

S. cerevisiae. • We determined values for MIC of STS CONDITIONER for the bacteria and

the other contaminant in this sample to be 0.05 and 0.5, respectively. • We added yeast and STS nutrients and determined the MIC of STS

CONDITIONER for the yeast.• The results show that STS CONDITIONER impacts yeast propagation at

levels of 0.5% and above, even in the presence of STS nutrients.


• Following this series we spiked a sample with 2 x 106 cells of bacteria (lactobacillus fermentum) and ran yeast (Saccharomyces cerevicea) propagation trials in the presence of various concentrations of STS Conditioner, Lactrol, and Isostab.

• The concentrations of Lactrol and Isostab were chosen based on the recommended values (Lactrol 0.5 ppm, Isostab 50 ppm) in the product literature.

• Controls involving yeast only and bacteria only were also included. The concentrations of the test solutions were:

STS Conditioner

0.5 %(5,000 ppm)

0.1%(1,000 ppm)

0.05%(500 ppm)

Lactrol 1 ppm 0.5 ppm 0.25 ppm

Isostab 100 ppm 50 ppm 25 ppm


• Total number of baffled flasks - 12 • Final volume in flasks: 50ml = 40ml (w/v) corn mash + 10ml dewater • pH of the corn mash + yeast samples was measured before and after

addition of the test solutions, and also at 3 subsequent time points for all samples

• Ave initial pH 5.9 for all samples before the addition of test solutions • pH for Lactrol and IsoStab decreased on average to pH 5.3

• pH for STS Conditioner @ 0.50% increased 4.58 to 5.3 @ 0.10% increased 5.23 to 5.3 @ 0.05% decreased 5.78 to 5.35


• For the case of Lactrol addition to samples containing yeast and bacteria, photos of the plates at the three Lactrol concentrations were shown.

• Figure 1, show a slight decrease in the colonies (presumably a decrease in bacterial colonies) as a function of Lactrol concentration. – Little or no variation was observed for Isostab in a similar trial.

• For the case of STS Conditioner addition to samples containing yeast and bacteria, photos of the plates at the three STS Conditioner concentrations are shown in Fig 2.

Figure 1 Figure 2


• Qualitatively, from the growth on the plates it is visually apparent that STS Conditioner at 0.5% is more effective at killing the bacteria than Lactrol at 1 ppm under these conditions. – However, when both the yeast and lactobacillus are present at high numbers so it is

difficult to distinguish them and make quantitatively accurate counts.

STS Conditioner softens LigninsApplied to Cellulosic Scrap: No heat, 1 week exposure

STS Conditioner softens Lignins Applied to Shredded computer paper: No heat, 90 second exposure

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Sts conditioner, a product in development with large potential