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Strata SPE Sample Preparation Products
• CleanExtracts• HigherRecovery• BetterReproducibility
Guaranteed Performance OurStrataSPEproductlineembodiesqualityandperformance.Itsextensivequalitycontrolprocedure
providestrouble-freesolidphaseextractionmethods.AllStrataSPEphasesandformatsaretheresultof
asophisticatedmanufacturingprocess-eliminatingvariability.
EveryproductundergoescomprehensiveQCtestingtoensurereproducibleproductmanufacturing
• Consistentflowcharacteristics
• Consistentphaseselectivity
• Inertsorbentandmaterials
Cross Reference
©2010Phenomenex,Inc.Allrightsreserved.
If Strata SPE products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 45 days and keep the Strata SPE product for FREE!
Phenomenex Strata
Waters Sep-Pak
Varian Bond Elut
Supelco Discovery UCT
JT BAKER Bakerbond
IST ISOLUTE
Macherey-Nagel Chromabond
Reversed PhaseC18-E tC18 C18 DSC-18 C18 Octadecyl C18(EC) C18ecC18-U C18-OH PolarPlus
OctadecylLightloadC18 C18
C18PAHC18-T C18 C18-EWP DStC-18LtC8 C8 C8 DSC-8 C8 Octyl C8(EC) C8Phenyl(PH) PH DSC-PH Phenyl PhenylSDB-L ENV
LMSDSC-PS/DVB StyreScreen®DVB H2O-phobicDVB 101 HR-P
Normal PhaseSi-1(Silica) Silica Silica DSC-Silica Silica SilicaGel Silica SiOHFL-PR(Florisil®) Florisil® Florisil® Florisil® Florisil® Florisil® FL Florisil®
NH2 NH2 NH2 DSC-NH2 AminoPropyl Amino NH2 NH2
CN CN CN-U DSC-CN CN Cyano CN CNIon ExchangeABWSAX SAX DSC-SAX QuaternaryAmine QuaternaryAmine SAX SBSCX SCX DSC-SCX BenzeneSulfonic
AcidAromaticSulfonicAcid
SCX SA
WCX CBA DSC-WCX CarboxylicAcid CarboxylicAcid CBA PCAScreen-C CertifyI CleanScreen
DAUNarc-2 HCX Drug
Screen-CGF CertifyHF XtractDAUScreen-A CertifyII CleanScreenTHC Narc-1 HAX
2 Phenomenex|Web:www.phenomenex.com
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High Quality Economical Product Designed to Meet Your Sample Preparation Needs
Inert material leads to cleaner extracts Inthiscomparisontest,StrataC18-Egivescleanerextractsthanmanufacturer’salternativesolutions.
Contact Phenomenex for details regarding this method. Comparative separations may not be representative of all applications.
Consistent manufacturing and QC ensures reproducible resultsRecoverydatafor5differentlotsofStrataC18-E200mg/3mL
Certificate of Analysis included with every box of Strata
NADOLOL
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PROPRANOLOL
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Phenomenex Strata® C18-E
Waters® Sep-Pak® C18 IST ISoluTE® C-18(EC) Varian® Bond Elut® C18
Improve your results now with a change to StrataCombiningover25yearsofindustryknowledgeandchromatographyexperience,PhenomenexhasdevelopedtheStrataSPElinewithinourextensiveSamplePreparationproductrange.StrataSPEencompassesavarietyofhighqualitysorbentsandformatsdesignedtoeffectivelycleanupandconcentratesamplesfrommatricesencounteredinallindustries.
Phenomenex|Web:www.phenomenex.com
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Determining Proper Bed MassSorbent Bed Mass = Analyte Capacity (i.e.30mg,50mg,60mg,100mg,150mg,200mg,500mg,1g,2g,5g,10g,20g,50g,70g)Thesorbentbedmassdeterminesthemaximumamountofanalytethatcanberetainedbythesorbent.
Silica-based sorbents Non-ionicsorbentsretainamassofsolute(analyteplusretainedcontaminants)thatisequivalenttoapproximately5%ofthesorbentmass.Therefore,a100mgcartridgecanretainapproximately5mgoftotalsolutemass.
Ion-exchange sorbents Capacityisdeterminedbythenumberofchargedsitesonthesorbentavailabletointeractwiththeanalyte.
Suggested Loading CapacitySilica-Based Sorbents (StrataC18-E,C8,SCX,SAX,WCX,NH2,etc.)
Selecting the Right Strata SPE Product• Sorbentbedmass
• Reservoirvolume
• LoadingCapacity
• Wash/elutionvolume
Sample Matrix Sorbent MassBlood,serum,plasma 50mgsorbentper250µLUrine 50mgsorbentper500µLFilteredtissuehomogenates 100mgsorbentper100mgtissue
Environmental Samples Sorbent MassWater(particulate-free)drinking 500mg/100-500mLsampleWater(particulate-laden)rivers,runoff,etc.
1g/100-500mLsample
SoilExtracts 1g/100gofsoilextract
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Reduce breakthrough by selecting the proper bed mass!
Sorbent Wash & Elution Volumes*
ThevolumeofsolventneededforSPEprocessingisdirectlyrelatedtothemassofsorbentintheSPEtube,andmorespecifically,the“bedvolume”oftheSPEdevice.Intuitivelyweknowmoresorbentrequiresmoresolvent,lesssorbent=lesssolvent.Typically,4–16bedvolumesareusedinSPEmethods.
Reservoir = sample volume capacity(i.e.1mL,3mL,6mL,12mL,20mL,60mL,150mL)ThecolumnvolumereferstothedimensionsoftheSPEhousing(i.e.tube).Itdeterminesthelargestvolumesolventorsamplethatcanbedeliveredperaliquot.
Silica- BasedSorbent Mass
Practical Minimum Wash and Elution Volume 4 bed volumes
Recommended Wash and Elution Volume 8 bed volumes
Polymer- Based Sorbent Mass*
Practical Minimum Wash and Elution Volume 4 bed volumes
Recommended Wash and Elution Volume 8 bed volumes
10mg 60µL 120µL 10mg 100µL 200µL— — — 30mg 300µL 600µL50mg 300µL 600µL — — —— — — 60mg 600µL 1.2mL100mg 600µL 1.2mL 100mg 1mL 2mL150mg 900µL 1.8mL 150mg 1.5mL 3mL200mg 1.2mL 2.4mL 200mg 2mL 4mL500mg 3mL 6mL 500mg 5mL 10mL1g 6mL 12mL 1g 10mL 20mL2g 12mL 24mL — — —5g 30mL 60mL — — —10g 60mL 120mL — — —
*StratapolymericresinshavealargersurfaceareathanStratasilica-basedmaterialhencerequiringslightlymoresolventpergramforprocessing.Theelutionvolumesarespecifictothechemicalnatureoftheanalytebeingextracted,itsconcentrationinthesample,thechemicalnatureoftheelutingsolventandthebedmassused.Theaboveisaguideline,anelutionstudyshouldbeconductedtodeterminetheappropriatevolumetouse.
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Selecting the Proper Strata Phase
Learn More About Our Strata PhasesReversed Phase Benefits Typical ApplicationC18-E Extractionofhydrophobicmoleculesfromaqueousandbiologicalsamples.
Extraction of hydrophobic or polar organic analytes from
aqueous matrix
C18-U Increasedextractionefficiencyandenhancedcleanupofhydrophobiccompoundsthatcontainhydroxyoraminefunctionalgroupsfromwaterorbiologicalfluids.
C18-T Widepore.Extractionoflargehydrophobicmolecules(upto75kD)fromwaterorbiologicalmatrices.
C8 ExtractionofhydrophobiccompoundsfromwaterorbiologicalfluidsthatareretainedtoostronglyonStrataC18-EorStrata-X.
Phenyl Extractionofaromatichydrophobiccompounds.CN Extractionofnon-polar,compoundsthatareretainedtoostronglyonStrataC18-EorC8.SDB-L Styrene-divinylbenzene.Extractionofnon-polarandpolarmolecules.Normal Phase Benefits Typical ApplicationCN Normalphasesorbentthatcaneffectivelyextractpolarcompoundsfromnon-polarsolvents.
Extraction of polar analytes from non-polar organic
solvents
NH2 Extractionofstronganionsfromaqueoussamples.EPH Fractionationofaliphaticandaromaticextractablehydrocarbonsfromenvironmentalsamples.Silica Extractionofpolarcompoundsthataresimilarinstructure.Florisil®(FL-PR) Extractionofpesticidesfromenvironmentalsamples.Alumina-N(AL-N) Extractionofpolarcompoundsfromfoodandenvironmentalsamples.Eco-Screen Extractionofnaphthalenefromenvironmentalsamples.Ion-Exchange Benefits Typical ApplicationWCX Weakcationexchange.Extractionofquaternaryamines.
Extraction of charged analytes from aqueous or non-polar
organic samples
SCX Strongcationexchange.Extractionof1°,2°and3°aminesfrombiologicalfluids.Screen-C Mixedmodecationexchange.Extractionofbasicdrugsfrombiologicalmatricessuchasblood,
serumandurine.WAX Weakanionexchange.Extractionofstrongand/orweakanionsfromaqueoussolvent.SAX Stronganionexchange.Extractionoforganicacids.Screen-A Mixedmodeanionexchange.Extractionofacidicdrugsfrombiologicalmatricessuchasblood,
serumandurine.ABW Specialtyphase.Fractionationofneutralcompoundssuchasamidesfromacidicandbasic
analytes.
Strata Selection Treeaqueous
water
ionic
organic
organic
neutral
organic
neutral
nonpolar
reversedphase
SDB-L
C18-E
C8
PH
(+)basic
cationexchange
SCX
WCX
Screen-C
Screen-C GF
(-)acidic
anionexchange
SAX
NH2
Screen-A
polar
reversedphase
CN
neutral
moderatelypolar
reversedphase
C8
CN
C18-U
nonpolar
reversedphase
SDB-L
C18-E
C8
PH
moderatelypolar
normalphase
Si-1
NH2
EPH
Eco-Screen
AL-N
polar
normalphase
CN
Si-1
FL-PR
NH2
(+)basic
cationexchange
SCX
WCX
Screen-C
Screen-C GF
(-)acidic
anionexchange
SAX
NH2
Screen-A
ionic
Phenomenex|Web:www.phenomenex.com
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For On-line Screening• 1-3minuteruntime
• Directinjectanalysis
For Traditional Sample Preparation
•Compatiblewithmostmanifoldsandroboticworkstations
•Consistentwell-to-wellandtube-to-tubeflow
For Flash Analysis•Widerangeofpolarandnon-polar
selectivities
•Gramtokilogramquantities
For Flash and Large Scale-up
•Availablein12,20,60,and150mLvolumetubesizes
•Pre-packedformatseliminateexcesslaborassociatedwithglasspackedcolumns
Quality Products
Phenomenex|Web:www.phenomenex.com
If Strata SPE products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 45 days and keep the Strata SPE product for FREE!
Strata C18-ESorbent Mass Part No. Unit/Box PriceTube50mg 8B-S001-DAK 1mL(100/Box)100mg 8B-S001-EAK 1mL(100/Box)100mg 8B-S001-EBJ 3mL(50/Box)200mg 8B-S001-FBJ 3mL(50/Box)200mg 8B-S001-FCH 6mL(30/Box)500mg 8B-S001-HBJ 3mL(50/Box)500mg 8B-S001-HCH 6mL(30/Box)Giga™ Tube500mg 8B-S001-HDG 12mL(20/Box)2g 8B-S001-KDG 12mL(20/Box)5g 8B-S001-LEG 20mL(20/Box)10g 8B-S001-MFF 60mL(16/Box)20g 8B-S001-VFF 60mL(16/Box)50g 8B-S001-YSN 150mL(8/Box)70g 8B-S001-ZSN 150mL(8/Box)96-Well Plate25mg 8E-S001-CGB 2Plates/Box50mg 8E-S001-DGB 2Plates/Box100mg 8E-S001-EGB 2Plates/Box
On-Line Extraction Cartridge
Description Part No.Unit/Box Price
StrataC18on-lineextractioncartridge,20x2.0mm
00M-S039-B0-CB
ea
Cartridgeholder,20mm
CH0-5845 ea
Strata C8Sorbent Mass Part No. Unit/Box PriceTube100mg 8B-S005-EAK 1mL(100/Box)200mg 8B-S005-FBJ 3mL(50/Box)500mg 8B-S005-HBJ 3mL(50/Box)500mg 8B-S005-HCH 6mL(30/Box)1g 8B-S005-JCH 6mL(30/Box)Giga™ Tube2g 8B-S005-KDG 12mL(20/Box)5g 8B-S005-LEG 20mL(20/Box)10g 8B-S005-MFF 60mL(16/Box)96-Well Plate25mg 8E-S005-CGB 2Plates/Box50mg 8E-S005-DGB 2Plates/Box100mg 8E-S005-EGB 2Plates/Box
On-Line Extraction Cartridge
Description Part No.Unit/Box Price
StrataC8on-lineextractioncartridge,20x2.0mm
00M-S101-B0-CB
ea
Cartridgeholder,20mm
CH0-5845 ea
Strata C18-USorbent Mass Part No. Unit/Box PriceTube100mg 8B-S002-EAK 1mL(100/Box)200mg 8B-S002-FBJ 3mL(50/Box)500mg 8B-S002-HBJ 3mL(50/Box)1g 8B-S002-JCH 6mL(30/Box)96-Well Plate50mg 8E-S002-DGB 2Plates/Box100mg 8E-S002-EGB 2Plates/Box
Strata PhenylSorbent Mass Part No. Unit/Box PriceTube100mg 8B-S006-EAK 1mL(100/Box)200mg 8B-S006-FBJ 3mL(50/Box)500mg 8B-S006-HBJ 3mL(50/Box)500mg 8B-S006-HCH 6mL(30/Box)1g 8B-S006-JCH 6mL(30/Box)96-Well Plate25mg 8E-S006-CGB 2Plates/Box50mg 8E-S006-DGB 2Plates/Box100mg 8E-S006-EGB 2Plates/Box
Strata SDB-LSorbent Mass Part No. Unit/Box PriceTube100mg 8B-S014-EAK 1mL(100/Box)200mg 8B-S014-FBJ 3mL(50/Box)200mg 8B-S014-FCH 6mL(30/Box)500mg 8B-S014-HBJ 3mL(50/Box)500mg 8B-S014-HCH 6mL(30/Box)1g 8B-S014-JCH 6mL(30/Box)Giga™ Tube2g 8B-S014-MFF 60mL(16/Box)96-Well Plate50mg 8E-S014-DGB 2Plates/Box
Strata CN (canalsobeusedforNormalPhase)
Sorbent Mass Part No. Unit/Box PriceTube100mg 8B-S007-EAK 1mL(100/Box)200mg 8B-S007-FBJ 3mL(50/Box)500mg 8B-S007-HBJ 3mL(50/Box)500mg 8B-S007-HCH 6mL(30/Box)1g 8B-S007-JCH 6mL(30/Box)Giga Tube2g 8B-S007-KDG 12mL(20/Box)96-Well Plate25mg 8E-S007-CGB 2Plates/Box50mg 8E-S007-DGB 2Plates/Box100mg 8E-S007-EGB 2Plates/Box
Strata C18-T (widepore)
Sorbent Mass Part No. Unit/Box PriceTube100mg 8B-S004-EAK 1mL(100/Box)200mg 8B-S004-FBJ 3mL(50/Box)500mg 8B-S004-HBJ 3mL(50/Box)500mg 8B-S004-HCH 6mL(30/Box)1g 8B-S004-JCH 6mL(30/Box)96-Well Plate25mg 8E-S004-CGB 2Plates/Box50mg 8E-S004-DGB 2Plates/Box
Reversed Phase
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Terms and Conditions SubjecttoPhenomenexStandardTermsandConditions,whichmaybeviewedatwww.phenomenex.com/TermsAndConditions
Trademarks StrataisaregisteredtrademarkofPhenomenex,Inc.Giga,Sepra,andStrata-XaretrademarksofPhenomenex,Inc.BondElutisaregisteredtrademarkofVarian,Inc.StyreScreenisaregisteredtrademarkofUCT.Sep-PakisaregisteredtrademarkofWatersCorp.FlorisilisaregisteredtrademarkofU.S.SilicaCo.ISOLUTEisaregisteredtrademarkofBiotage.StyreScreenisaregisteredtrademarkofUCT.
Disclaimer Phenomenex,Inc.isinnowayaffiliatedwithanycompanieslistedabove.Comparativeseparationsmaynotberepresentativeofallapplications.
©2010Phenomenex,Inc.Allrightsreserved.
Phenomenex|Web:www.phenomenex.com
Strata ABW (specialtyphase)Sorbent Mass Part No. Unit/Box PriceTube1g 8B-S030-JCH 6mL(30/Box)Giga™ Tube2g 8B-S030-KDG 12mL(20/Box)5g 8B-S030-LEG 20mL(20/Box)10g 8B-S030-MFF 60mL(16/Box)20g 8B-S030-VFF 60mL(16/Box)
Strata Silica (Si-1)
Sorbent Mass Part No. Unit/Box PriceTube100mg 8B-S012-EAK 1mL(100/Box)200mg 8B-S012-FBJ 3mL(50/Box)500mg 8B-S012-HBJ 3mL(50/Box)500mg 8B-S012-HCH 6mL(30/Box)1g 8B-S012-JCH 6mL(30/Box)1g 8B-S012-JDG 12mL(20/Box)Giga™ Tube500mg 8B-S012-HDG 12mL(20/Box)2g 8B-S012-KDG 12mL(20/Box)5g 8B-S012-LEG 20mL(20/Box)10g 8B-S012-MFF 60mL(16/Box)20g 8B-S012-VFF 60mL(16/Box)50g 8B-S012-YSN 150mL(8/Box)70g 8B-S012-ZSN 150mL(8/Box)96-Well Plate25mg 8E-S012-CGB 2Plates/Box50mg 8E-S012-DGB 2Plates/Box100mg 8E-S012-EGB 2Plates/Box
Strata SCX (strongcationexchange)
Sorbent Mass Part No. Unit/Box PriceTube100mg 8B-S010-EAK 1mL(100/Box)100mg 8B-S010-EBJ 3mL(50/Box)200mg 8B-S010-FBJ 3mL(50/Box)500mg 8B-S010-HBJ 3mL(50/Box)500mg 8B-S010-HCH 6mL(30/Box)1g 8B-S010-JCH 6mL(30/Box)Giga™ Tube2g 8B-S010-KDG 12mL(20/Box)5g 8B-S010-LEG 20mL(20/Box)10g 8B-S010-MFF 60mL(16/Box)20g 8B-S010-VFF 60mL(16/Box)96-Well Plate25mg 8E-S010-CGB 2Plates/Box50mg 8E-S010-DGB 2Plates/Box100mg 8E-S010-EGB 2Plates/Box
Strata Screen-A (mixed-modeanionexchange)
Sorbent Mass Part No. Unit/Box PriceTube100mg 8B-S019-EAK 1mL(100/Box)200mg 8B-S019-FBJ 3mL(50/Box)200mg 8B-S019-FCH 6mL(30/Box)500mg 8B-S019-HCH 6mL(30/Box)96-Well Plate25mg 8E-S019-CGB 2Plates/Box
Strata Screen-C (mixed-modecationexchange)
Sorbent Mass Part No. Unit/Box PriceTube100mg 8B-S016-EAK 1mL(100/Box)100mg 8B-S016-EBJ 3mL(50/Box)150mg 8B-S016-SBJ 6mL(30/Box)150mg 8B-S016-SCH 6mL(30/Box)200mg 8B-S016-FBJ 3mL(50/Box)300mg 8B-S016-RBJ 3mL(50/Box)500mg 8B-S016-HCH 6mL(30/Box)
Strata EPH (specialtyphase)
Sorbent Mass Part No. Unit/Box PriceTube500mg 8B-S031-HBJ 3mL(50/Box)Giga™ Tube5g 8B-S031-LEG 20mL(20/Box)Teflon Giga™ Tube5g 8B-S031-LEG-T 20mL(20/Box)
Strata NH2 / WAX (canalsobeusedforAnionExchange)
Sorbent Mass Part No. Unit/Box PriceTube100mg 8B-S009-EAK 1mL(100/Box)200mg 8B-S009-FBJ 3mL(50/Box)500mg 8B-S009-HBJ 3mL(50/Box)500mg 8B-S009-HCH 6mL(30/Box)1g 8B-S009-JCH 6mL(30/Box)Giga™ Tube500mg 8B-S009-HDG 12mL(20/Box)2g 8B-S009-KDG 12mL(20/Box)5g 8B-S009-LEG 20mL(20/Box)10g 8B-S009-MFF 60mL(16/Box)20g 8B-S009-VFF 60mL(16/Box)96-Well Plate25mg 8E-S009-CGB 2Plates/Box50mg 8E-S009-DGB 2Plates/Box100mg 8E-S009-EGB 2Plates/Box
Normal Phase Anion ExchangeStrata WCX (weakcationexchange)
Sorbent Mass Part No. Unit/Box PriceTube100mg 8B-S027-EAK 1mL(100/Box)200mg 8B-S027-FBJ 3mL(50/Box)500mg 8B-S027-HBJ 3mL(50/Box)500mg 8B-S027-HCH 6mL(30/Box)1g 8B-S027-JCH 6mL(30/Box)Giga™ Tube2g 8B-S027-KDG 12mL(20/Box)5g 8B-S027-LEG 20mL(20/Box)10g 8B-S027-MFF 6mL(16/Box)96-Well Plate25mg 8E-S027-CGB 2Plates/Box50mg 8E-S027-DGB 2Plates/Box100mg 8E-S027-EGB 2Plates/Box
Cation ExchangeStrata SAX (stronganionexchange)Sorbent Mass Part No. Unit/Box PriceTube100mg 8B-S008-EAK 1mL(100/Box)100mg 8B-S008-EBJ 3mL(50/Box)200mg 8B-S008-FBJ 3mL(50/Box)500mg 8B-S008-HBJ 3mL(50/Box)500mg 8B-S008-HCH 6mL(30/Box)1g 8B-S008-JCH 6mL(30/Box)Giga™ Tube500mg 8B-S008-HDG 12mL(20/Box)2g 8B-S008-KDG 12mL(20/Box)5g 8B-S008-LEG 20mL(20/Box)10g 8B-S008-MFF 60mL(16/Box)20g 8B-S008-VFF 60mL(16/Box)96-Well Plate25mg 8E-S008-CGB 2Plates/Box50mg 8E-S008-DGB 2Plates/Box100mg 8E-S008-EGB 2Plates/Box
Strata Florisil®
(pesticideresiduegrade)
Sorbent Mass Part No. Unit/Box PriceTube500mg 8B-S013-HBJ 3mL(50/Box)500mg 8B-S013-HCH 6mL(30/Box)1g 8B-S013-JCH 6mL(30/Box)Giga™ Tube1g 8B-S013-JEG 20mL(20/Box)2g 8B-S013-KDG 12mL(20/Box)5g 8B-S013-LEG 20mL(20/Box)10g 8B-S013-MFF 60mL(16/Box)
Strata CN(SeeunderReversedPhasefororderinginformation)
Strata NH2 / WAX(SeeunderNormalPhasefororderinginformation)
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