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Tom Wurdinger
Cancer Center Amsterdam
VU University Medical School
STOPhersentumoren Symposium
Preclinical brain tumor models
Primary glioma cells expressing Firefly
luciferase and fluorescent mCherry
Firefly luciferase / mCherry
Preclinical glioma models
Preclinical glioma models
WEE1 inhibitor: PD0166285
Control AZD1775 IR IR + AZD1775
AZD1775
Phase I/II trial
NCT01849146
WEE1 inhibitor: AZD1775 (NCT01849146)
MPS1 inhibitor: preclinical drug discovery
U87R1 tumor volumeU87 tumor volume U87 survival U87R1 survival
TMZ sensitizers: Hydroxyurea
2000-compound FDA drug screen
Hydroxyurea
Phase I/II trial
scheduled
CTRL
TMZ
RO
TMZ+RO
Day 6 Day 10 Day 13 Day 16 RLU
Click # LH2013061013502510 Jun 2013 13:50:38Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 247 m1,2,3Experiment: Hs683 FMLabel: Comment: Analysis Comment:
5
6
7
8
910
5
2
3
4
5
6
7
8
910
6
Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmicClick # LH20130614165739
14 Jun 2013 16:57:52Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 247 m1,2,3Experiment: Hs683 FMLabel: Comment: Analysis Comment:
5
6
7
8
910
5
2
3
4
5
6
7
8
910
6
Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Click # LH2013061710200617 Jun 2013 10:20:19Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 247 m1,2,3Experiment: Hs683 FMLabel: Comment: Analysis Comment:
5
6
7
8
910
5
2
3
4
5
6
7
8
910
6
Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Click # LH2013062016081620 Jun 2013 16:08:27Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 259 m1,2,3Experiment: Hs683 FMLabel: Comment: Analysis Comment:
5
6
7
8
910
5
2
3
4
5
6
7
8
910
6
Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Click # LH2013061013340410 Jun 2013 13:34:18Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 246 m1,2,3Experiment: Hs683 FMLabel: Comment: Analysis Comment:
5
6
7
8
910
5
2
3
4
5
6
7
8
910
6
Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmicClick # LH20130614163904
14 Jun 2013 16:39:17Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 246 m1,2,3Experiment: Hs683 FMLabel: Comment: Analysis Comment:
5
6
7
8
910
5
2
3
4
5
6
7
8
910
6
Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Click # LH2013061710040917 Jun 2013 10:04:22Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 246 m1,2,3Experiment: Hs683 FMLabel: Comment: Analysis Comment:
5
6
7
8
910
5
2
3
4
5
6
7
8
910
6
Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Click # LH2013062016243520 Jun 2013 16:24:48Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 260 m1,2,3Experiment: Hs683 FMLabel: Comment: Analysis Comment:
5
6
7
8
910
5
2
3
4
5
6
7
8
910
6
Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Click # LH2013061013260210 Jun 2013 13:26:16Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 245 m4,5,6Experiment: Hs683 FMLabel: Comment: Analysis Comment:
5
6
7
8
910
5
2
3
4
5
6
7
8
910
6
Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Click # LH2013061416301514 Jun 2013 16:30:29Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 245 m4,5,6Experiment: Hs683 FMLabel: Comment: Analysis Comment:
5
6
7
8
910
5
2
3
4
5
6
7
8
910
6
Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Click # LH2013061709551817 Jun 2013 09:55:31Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 245 m4,5Experiment: Hs683 FMLabel: Comment: Analysis Comment:
5
6
7
8
910
5
2
3
4
5
6
7
8
910
6
Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Click # LH2013061909360319 Jun 2013 09:36:16Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 257 m3,4Experiment: Hs683 R1 FMLabel: Comment: Analysis Comment:
5
6
7
8
910
5
2
3
4
5
6
7
8
910
6
Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Click # LH2013061013071910 Jun 2013 13:07:32Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 244 m4,5,6Experiment: Hs683 FMLabel: Comment: Analysis Comment:
5
6
7
8
910
5
2
3
4
5
6
7
8
910
6
Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Click # LH2013061416113814 Jun 2013 16:11:51Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 244 m4,5,6Experiment: Hs683 FMLabel: Comment: Analysis Comment:
5
6
7
8
910
5
2
3
4
5
6
7
8
910
6
Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Click # LH2013061709390317 Jun 2013 09:39:17Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 244 m5,6Experiment: Hs683 FMLabel: Comment: Analysis Comment:
5
6
7
8
910
5
2
3
4
5
6
7
8
910
6
Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Click # LH2013062016001920 Jun 2013 16:00:32Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 258 m5,6Experiment: Hs683 R1 FMLabel: Comment: Analysis Comment:
5
6
7
8
910
5
2
3
4
5
6
7
8
910
6
Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Click # LH2013061710200617 Jun 2013 10:20:19Bin:M (4), FOV12.5, f1, 1mFilter: OpenCamera: IVIS 11215, DW434
Series: cage 247 m1,2,3Experiment: Hs683 FMLabel: Comment: Analysis Comment:
5
6
7
8
910
5
2
3
4
5
6
7
8
910
6
Color BarMin = 50000Max = 1e+06
bkg subflat-fieldedcosmic
Phase I/II trial
ongoing
NCT01119599
TMZ sensitizer: RO4929097 (NCT01119599)
TAKE HOME MESSAGE: NEW THERAPIES ON THE HORIZON
Hydroxyurea
Phase I/II trial
Scheduled
RO4929097
Phase I/II trial
NCT01119599
MPS1i
Preclinical study
Ongoing
AZD1775
Phase I/II trial
NCT01849146
Rindopepimut NCT01480479
Valganciclovir (Valcyte; FDA approved)
Bevacizumab (Avastin; FDA approved)
BRAIN TUMOR CENTER AMSTERDAM
Neuro-oncology Research Group
VU University medical center | Cancer Center Amsterdam
Tom Wurdinger
Cancer Center Amsterdam
VU University Medical School
STOPhersentumoren Symposium