Recombinant DNAStephanie Bryce Sierra WTyler

WOW! This is Awesome

Sociologist

One Perspective

• One way people look at recombinant DNA is that we are altering what God has created. In other

words, we are acting in the way as God.• Some people say if we alter what we are made from, should it even have been made in the first

place?• Also, is it ethically and morally correct?

• They feel as though the changing of DNA will be hurting or make the product they are fixing worse.

Another Perspective

• Some people also think that if we have the technology and the man power to be able to do

such things, we should do it.• If this will change the society today, it is the best

thing we can possibly do at this point.• This can change many people’s lives.

• Also, it could save many lives and help the world survive from many of the diseases it is

contaminated with.

Medicinal Perspective

• With Recombinant DNA, insulin can be made to be able to help people in need with diabetes

• Also, this is the way in which cheaper, off-brand medicines are made making most consumers of the product happy by having not to buy the most

expensive product

One Last Perspective

• Recombinant DNA can change the world• With the technology to do this, we could increase

the productivity of the crops we produce each year, which most people find as a great thing

• Another good thing they like is how scientists can make the nutritional value of any crop they want

to with this technology

Conclusion of Sociologist

• There are many different views and perspectives on recombinant DNA

• Many think we are altering the ways of God and believe it should be stopped

• Others believe if we are able to do such a thing, we should do it.

• What is your view on recombinant DNA?

Lawyer

Laws• Any institution that sponsors recombinant DNA

research that is covered by the National Institutes of Health (NIH) guidelines must make sure that the appropriate level of review occurs by the Institutional Biosafety committee, and that any required petitions are submitted to the NIH.

• Any experiments must be reviewed by the institution.

Safety Laws• Employers do not protect employees or prioritize

public safety until compelled by law.

• Employees who are exposed or become seriously ill from genetically-engineered infectious agents have no rights to exposure records and materials for their medical evaluation and treatment even after having physicians request this information for their healthcare. This is due to the biomedical company doesn’t want information released to the public.

More Safety laws• There is no law that requires them to address

safety concerns for scientists with dangerous technology.

Member Laws• The IBC must have at least five members. No

fewer

• Two members must not be affiliated with the institution who represent the interest of the surrounding community with respect to protection of the health and safety of the environment.

Report Laws• The institution must file an annual report that

includes the following:1.An updated committee roster indicating the role

of each committee member and2.Bio sketches for each member of the committee.

This can be a CV or resume.

• Cover letter should indicate that the info is being submitted as the IBC’s annual report.

More Report Laws• They must notify OBA of any changes to the

committee when they occur. If they do occur the report should have the change and the information and resume of the new member(s).

• They should also state any significant problems or violations of the NIH guidelines or any research related accidents or illnesses to NIH OBA within 30 days.

Protective Laws• Intellectual property is the term used to describe

the branch of law which protects the application of thoughts, ideas, and information which are of commercial value. It covers patents, copyrights, trademarks, trade secrets and other similar rights.

State Laws• Wisconsin made it so the Wisconsin Department

of Natural Resources (WDNR) or Department of Agriculture, Trade and Consumer Protection be notified of intended field studies of genetically engineered organisms.

Scientists

What is Recombinant DNA?

Recombinant DNA is a type of artificially created DNA by inserting a stand or more of DNA into a different set of DNA.

How does Recombinant DNA work?There are 3 ways that Recombinant DNA can be made. The 3 ways that Recombinant DNA can be made are Transformation, Phage Introduction, and Non-Bacterial Transformation.

Transformation

The process begins when a piece of DNA is selected to be inserted into a vector. The second step is to cut that piece of DNA with a restriction enzyme and then ligate the DNA insert into the vector with DNA Ligase. The insert piece contains a special marker which allows identification for the Recombinant DNA. An antibiotic marker is often used so the host cell without a vector dies when exposed to an antibiotic, and the host with the vector will live because it’s resistant.

Phage Introduction

Phage Introduction is the process of transfection, which is also equivalent to transformation. A phage is inserted and used instead of a bacteria. In a vitro package a vector is used. This uses MI3 phages to produce phage plaques which contain recombinants. The recombinants that are created can be identified by differences in the recombinants and non-recombinants using various selection methods.

Non-Bacterial Transformation

This is a process very similar to Transformation the only difference between the two is non-bacterial does not use bacteria such as E. Coli for the host. • In microinjection, the DNA is injected directly into

the nucleus of the cell being transformed. • In biolistic, the host cells are bombarded with high

velocity micro projectiles, such as particles of gold or tungsten that have been coated with DNA.

Links• http://

agbiosafety.unl.edu/education/whowants.htm• http://

www.superteachertools.com/jeopardyx/jeopardy-review-game.php?gamefile=1297557028

• http://webapps.css.udel.edu/biotech/rDNA.html• http://www.ehow.com/video_4790967_recombinan

t-dna-technology_.html

• http://www.surveymonkey.com/s/QJWXKTM