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Step-Up Year 1 Practical Laboratory Session: Iron Analysis . Iron (Fe) . Iron is vital for a number of physiological processes Occurs naturally in food sources Also occurs naturally in our water . Iron in our water . Should we be concerned? - PowerPoint PPT Presentation
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Step-Up Year 1
Practical Laboratory Session: Iron Analysis
Iron (Fe) Iron is vital for a number of physiological processes
Occurs naturally in food sources
Also occurs naturally in our water
Iron in our water Should we be concerned?
Iron in water is found in two forms: - Ferrous (Fe2+)- Ferric (Fe3+)
Fe3 + 3H2O Fe(OH)3 + 3H+
Iron in our water A range of solutions from no iron to high iron content
Ferrous iron in water has addedto it thiocyanate (SCN)
Fe(SCN)(H20)5]2+
Iron in our water How do we decide what
should be classed as ‘low’ or ‘high’ iron content?
Calibration Curve – our baseline to compare against
Iron Analysis
Using a Spectrophotometer at a wavelength 490nm to measure Absorbance (nm)
Using the calibration curve, we can determine unknown
Iron Concentrations
Before you start Read the protocol thoroughly
?AnyQuestions
Always read the protocol before you start and follow H&S
Remember Beer-Lambert Law
Absorbance = ε.c.l
where ε = constantc = concentrationl = path length through solution