Stem Cells: Real Possibilities in Autism? by James Jeffrey Bradstreet, MD

8/3/2019 Stem Cells: Real Possibilities in Autism? by James Jeffrey Bradstreet, MD

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o eas, i have been studnthe mmune sstem n elatonto autsm, and i alwas comeback to the dea that t needsepoammn. i wsh t wee lkem compute so that i could ustpess the powe button untl testated aesh. But the mmune

sstem n autsm s plaued wth quk eactons tothe chlds own bod that s, autommunt, wth theban as ts specal taet.

ive ted a vaet o mmune aents to help oove a decade. Tese sometmes help; occasonall, as

wth ntavenous mmunolobuln iVig, the aetul estoatve. 1 But whee do we o when these

teatments dont eneate the esults and ecove ouchlden need?

sUppORT fOR ImmUNeCAUses Of AUTIsmpcall n medcne as we encounte somethn, wemove slowl and nd t challenn to accept chane.Doctos lke paadms and tadtons we nvest oulves n memozn s stems. Town somethnnew at medcne, lke autommune ban dseasepesentn as autsm, ust doesnt st well wth thestatus quo. Despte the odds aanst them, a ew bavethnkes ventue outsde the comot o the ex stn

paadm. in autsm, the mmune dseulaton

theo stated to emee wth Poesso gene Stubbsn the md-1970s. 2

Touh the dedcated eots o eseaches andclncans, we have slowl poessed, and now, aquate o a centu late, we have ou st textbookdenn the mmune-oxdatve stess lnkae toautsm,Autism: Oxidative Stress, Infammation, and

Immune Abnormalities.3

Fom the book summa:Autism: Oxidative Stress, Infammation,and Immune Abnormalities bnstoethe a wealth o cuttn-ede evdencethat s alead nuencn how we teat thsseous condton. it looks at the ole o

neuopatholocal abnomaltes, enetcs, andthose actos common to oxdatve stess suchas nammaton, mmune dsuncton, abeantcellula snaln , and ene-envonmentnteactons. Amon dozens o eseach topcs,ths volume Looks at nteactons between enetc and

envonmental actos such as the matenalmmune envonment and penatal/postnatalenvonmental stessos

Summazes evdence o oxdatve damaeand nammaton n autsm

intoduces a PDD behavo nvento as a

tool o assessn autsm

Consdes autsm as an abeant adaptveesponse to neuonammaton and oxdatvestess

Examnes the ole o abnomal calcumsnaln and the hpothess that t maepesent a taet o novel theapeutcs

Pesents a hpothess that autsm ases omthe dseulaton o a uned ut/bansstem athe than onatn n the banalone

Poposes the utlt o usn abopschosocal method to teat autsm

Ts book, edted b Chauhan, Chauhanand Bown, shows us that autsm s not onl

developmental but also a chonc condton basedon actve pathophsolo, and that t s not onlbehavoal but also pesents somatc and sstemceatues. Te ndns n these chaptes suppotthe theo that oxdatve stess plas an mpotantole n autsm. Te also pont to the value oconductn n-depth mechanstc studes as a wato uncove new taets o theapeutc nteventonn autsm.

About 4 eas ao i pesented and patcpatedn the scence oum that ultmatel led to thebooks publcaton t was an hono to be a pat ots bennns. But the eal hono oes to the late

Papa Bene rmland, who used the esouces o

(Note: This article was adapted rom inormation on my blog site www.drbradstreet.org, which is accessible to all and gives an option or regularupdates on the latest fndings on stem cells and the best o integrative medicine.)

sTem cells:

Real possiBiliTies

in auTism?By jAMES jEFFrEy Br ADSr EE, MD, MD(H), FAAFP

DR. JEFF BRADStREEt graduated rom the University o South Florida College o Medicine and received his residency trainingrom Wilord Hall USAF Medical Center. As a ight surgeon, he was involved in aerospace medicine research, and he has extensiveexperience and training in environmental medicine, hyperbarics, and toxicology. He is extensively published in autism research

and outcome studies and serves as an adjunct proessor o pediatrics at Southwest College o Naturopathic Medicine in Tempe,Arizona. Dr. Bradstreet is the ounder and director o the International Child Development Resource Center (.icdrc.org),which is located in Melbourne, Florida, with a satellite ofce in Irvine, Caliornia. His son, Matthew, is recovering rom autism withthe combined help o biomedical and behavioral interventions.


2/8www.autsmone.org REPRINTEDWITHPERMISSIONAUTISMSCIENCEDIGEST:THEJOURNALOFAUTISMONEISSUE01APRIL

the Autsm reseach insttute to und the meetn,and to D. Wood Mcgnns who coodnated thepoject on Benes behal.

ive also had the pleasue to patcpate wthPoesso Antono Pesco n numeous thnk tanksand autsm banstomn eteats. Dun thesesessons ive come to espect hs eseach and hsdese to nd the tuth. He honestl tes to emovebas om hs nvestatons and ask questons thatcan be answeed. Fo eas, ive been lectun on

m clncal obsevatons that autsm s elated tommune actvaton wth assocated oxdatve stess.Despte all the evdence we can pesent dun theselectues, i stll hea and ead n the meda that thees no evdence o an mmunolocal lnk to autsm. ismpl dont et t.

Te majot o clncans and scentsts havelael noed and not suppoted the conceptsllustated n the textbook. Now these ecentobsevatons o Po. Pesco and hs colleaues nital make t mpossble to den.

Gno-wid xprion tudi

in Auti pctru diordr, Rttndro, and Down ndro.Lintas C, Sacco R, Persico AM.Laboato o Molecula Pschat andNeuoenetcs, Unvest Campus Bo-Medco,rome, ital. Depatment o ExpementalNeuoscences, i.r .C.C.S. Fondazone SantaLuca, rome, ital.NeurobiolDis. 2010 Dec 2. [Epub ahead o pnt]

AbstractTouh deent n the aetolo, autsmspectum dsode (ASD), rett sndome

(r) and Down sndome (DS) ae theeneuodevelopmental dsodes shan sncantclncal and neuopatholocal ovelaps.genome-wde expesson studes ae evewedand avalable datasets om post-motem banseanalzed to dent enes and ene pathwasdseulated n all thee dsodes. Our resultssurprisingly converge upon immune, andnot neurodevelopmental genes, as themost consistently shared abnormalityin genome-wide expression patterns.A dysregulated immune response,accompanied by enhanced oxidative

stress and abnormal mitochondrialmetabolism seemingly represents thecommon molecular underpinning ofthese neurodevelopmental disorders. Tsconcluson ma be mpotant o the dentono phamacolocal theapes able to amel oateclncal smptoms acoss these dsodes.(Emphass added.)

So what does ths mean? Fo me ths speaks to acommon pathwa to neuolocal dsuncton dundevelopment. We know the development o the bans pmal eulated b mcolal cells see Fue 1.

figur 1: noral relatioship o iroglia to euros athe loo-rai arrier. creit: stanmed.stanford.edu

figur 2: Iproper utio o iroglia auses irease i the

toxi quiolii ai a irease oxiatie stress i the euro.not show here, ut learly part o the euroal threat, is irease

glutaate a reutio o glutathioe (a ritial atioxiat oreuros). creit: gladstone.ucsf.edu



Te mcolal cells ae the bans custom-mademmune cells. Te contnuall wok to shape, udeand pune the developn ban n a wa emnscent oa sklled vnedesse tendn the apevnes to poducethe nest wne. When t woks ht t s emakable.Howeve, we ae leann ths pocess s too easldsupted b nammaton. Tat s the messae i seen most o m patents and that s the messae omPescos team a s well.

But when the mcolal cells become dsunctonal(whethe wounded b toxns o tuned on b mmuneactvaton) the neuons ae n dane (see Fue 2).

INTRODUCTION TO sTem Cellsgven the ovewhelmn evdence o ban mmunedsuncton, the use o stem cells a s theapeutc aentsstats to become appealn. Te possess the potentalto eulate the mmune sstem (an ntnsc ole o stemcells), whch makes stem cell theap one o the ewoptons we have to edect ban mmune dsunctonapat om lon-tem mmunosuppessve aents. Andthee ae scentc easons to beleve multpotentaladult stem cells ma be able to coect the ononmmunolocal chaos chlden wth autsm expeence.

But beoe i et moe nto what stem cells aeand how the wok, i want to dspel a common

msconcepton: stem cells ae not iQ ponts n asne, no ae the a new ban lookn to happen.Tee s no wa o stem cells to become unctonal,matue ban cells that ae nteated nto the oveallccut wth othe ban cells. i know that s what

we all want o ou chlden wth autsm o ouandpaents wth Alzhemes, but t ust snt onto happen that wa. Even ou stuck the stem cellsdectl nto the ban, t s extemel unlkel to havean unctonal neuonal potental. Potocols usnnectons nto the ceebal spnal ud (CSF) va spnaltaps to nect the stem cells ae unusted. CSF sceated n the chood plexus n the ud-lled spaces

nsde the ban (see Fue 3). Te pessue ows omblood pessue dvn the chood ud poducton,to the ventcles, out o the skull thouh the oamenmanum (b hole at the bottom o the skull) and thendown the spnal cod. it does cculate n pat back tothe ban and t seems t s exchaned eve ew hous.But even wth t hs cculaton, we aent on to delvestem cells whee we want them.

We know om man anmal studes that stem cellsleave the bloodsteam and ente the ban.4 So the bloods the best and most local wa to et stem cells nto theban. But even once nsde, we can onl expect a eshmmune eulaton o the ban not a ebult cotex.

rht now ou ma be wonden wh we shouldbothe dscussn stem cells n elaton to autsm at all.And t weent o the onon nammato mess thatmost kds whom i see wth autsm sue om, then i

would aee wth ou. So, wh consde stem cells?

why CONsIDeR sTem

Cells fOR AUTIsm?

Fo a ew eas, oveseas stem cell centes have advetsedthe potental benets o stem cells o chlden wth

autsm. Teve set up opeatons n Costa rca (nowclosed), Panama, Mexco, Chna, the Domncanrepublc, geman and geece. Tese centes use a

vaet o cell tpes: etal, embonal, and mesenchmal.in most cases, the stem cells come om unknowndonos. Usn a ew anecdotes, the have tantalzed ouhopes that stem cells ma be the ultmate cue.

ive ntevewed patents beoe and ae teatmentswth stem cell theapes o a wde vaet o llnesseso the last 3 eas. Outsde the aena o autsm, iveseen some tul damatc postve chanes wth ceebalpals, heumatod athts, dabetes, deeneatve ontdsease and cosmetc sue. Studes n these aeas ae

makn the wa nto the scentc lteatue and ettnpesented at medcal coneences. yet n autsm, theeae no publshed cases to wok om, so we ae stll at theeal staes o ou use o ths nteventon. Despte that,thee ae cedble atonales o the applcaton o cetantpes o stem cell theapes to autsm.

We have mentoned the eat potental o stem cellsto eulate the mmune sstem, helpn to coect theonon mmunolocal chaos n chlden wth autsm.But what ae stem cells?

whAT ARe sTem Cells?

Stem cells ae an emen teatment opton that unlke medcatons ae capable o both epan andeeneatn the bod.

So what ae stem cells? Smpl put, these aepoento cells . . . somethn lke cell seeds. Anacon doesnt look lke an oak tee, but t contansthat potental. Stem cells ae potental matue cells.Te can ow nto matue cells n a pocess known asdeentaton. Ts ust means the chane om benmultpotental to ben ust one tpe o cell. (SeeFue 4.)

Stem cells possess the potental to eulate the mmune sstem (an ntnsc ole o stem cells), whchmakes stem cell theap one o the ew optons we have to edect ban mmune dsuncton apat om

lon-tem mmunosuppessve aents. And thee ae scentc easons to beleve multpotental adultstem cells ma be able to coect the onon mmunolocal chaos chlden wth autsm expeence.

figur 3: The rai a spial fui irulatio



Stem cells can be eneated om ou own bonemaow o adpose tssue (bod at) (see Fue 5).Tese ae called mesenchymalstem cells (autoloous =

sel-donated). Te can also be eneated n a smlapocess, but om a dono o at o bone maow(alloenec). in the Unted States ths s ou onl optonapat om patcpaton n specal eseach studes. inthe est o the wold, loc al ules appl, and ths vaesom count to count.

Te eneal cateoes o stem cells ae as ollows:

Embryonal: Deved om a human embo eneall om leove expanded blastocsts (6 to 8das) ae in vitro cultun. Souces ae etlt clncembo stoae centes (.e., the paents donated

unwanted etlzed es to eseaches). Tese aecapable o becomn human le mplanted nto aeceptve womb. Fo ths eason the use woldwdeemans contovesal. (Tese ae not pesentl leal touse n teatment n the US).

Fetal: As the name mples, these ae om abotons.Even moe than the embonal, use o these tpes ostem cells s hhl contovesal and pohbted n mancountes. i do NO use these, NOr do i ecommendthem, and the ae not all owed n the US.

Umbilical cord blood or placental: Tese canbe obtaned om amnocentess, the babs umblcalcod (UBSC), o the leove placenta (ae-bth).Cuentl thee ae lots o stoed umblcal cods. Anone umblcal cod b tsel wthout cultun s notsufcent to teat anone medcall. Some wok hasbeen done wth combnn multple umblcal cods(alloenec) to ceate enouh stem cells. Cuentl, weae not allowed to use stoed umblcal- deved stemcells o pl acentas. Fom the ndvduals pespectve,stoed UBSC s unlkel to be benecal. Collectvelthe ma be valuable to eseach o teatment, but thats stll qute a wa o. We ae not able to use UBSC atths tme.

Mulitipotential or mesenchymal stemcells (MSC): Tese have the potental toconvet to vaous cell tpes: muscle, at, catlae,

etc. Howeve, the main unctional role andreason or the therapeutic use o MSC istheir ability to reduce infammation and tosignal repair.

Whle makn moe at s sometmes anmpotant uncton and natuall occununctonal ole o stem cells (as n lln n deectsae nju), the exctn aspect o stem cell medcnes o the teatment o chonc nammato anddeeneatve dseases (e.., nammato boweldsease, autommune dsodes, athts, andneuolocal ssues).

Medcne s just at the onte o how to best

use MSC to help these dsodes, but ven thedevastatn complcatons o these d sodescombned wth the saet o MSC, the epesent

new and poweul tool n the ht aanst d sease.

fOCUs ON meseNChymAlsTem Cells: msCAs ou have seen, stem cells come n man tpes,but o m puposes, we ae talkn about adultstem cells. We use theadulttem to dstnush oembonc, etal, placental, cod blood, and smlatpes o cells. M specc nteest s n at, .e., adpodeved cells. Tese have mpotant dstnctons wthe own advantaes and dsadvantaes.

Adult MSC deved om ethe ad pose (at)o bone maow ae capable o anothe mpotant

figur 4: multipotential ste ells an eoe any ierent typeso ature ells. Tat is exatly wat tey o in etal evelopent. but istat wat we nee te to o in autis? I atually tink te role o ste

ells in autis is u ierent tan teir role in aking new tissue.

figur 5: Aipose ellsonverte ro aipose steells. Re roplets representstaine at insie te ells.

figur 6: Ste ell i the rai preetig ifaatio.

mesehyal Ste cell (mSc)

Etoeral cells mesoeral cells Eoeral cells

neuros(nere)

Aipose(at)

carti lage boe cariamusle

Skeletalmusle

Liercells

Pareaticells

internatonal Journal o infammaton. volue 2010, Arti le Id 151097, 18 pages



up the damaed tssue. Howeve, soon ae thateacton stats t snals local stem cells to poleate,and the mate to the wound. Once on ste, thecommuncate a calmn messae to nammatocells and stat to dect the epa pocess. Ts sexactl what we want them to do n the ban andthe choncall named ut o chlden wth autsm.We have alead seen an llustaton o stem actvtn the ban (Fue 6), and t s smla n the ut (seeFue 7 below, and abstact, next pae).

Fue 7 s complex, but t does a ood job odepctn the multple levels o contol the MSCcan exet ove nammaton. Sttn n the mddle

o Fue 7 s a MSC. it takes quckl ove themmune envonment, much lke a new eneal takescommand o a combat zone. Te MSC nceaseseulato counte-nammato e cells. it

educes mmune chemcals o nammaton ( NF-alpha, nteeon-amma, etc). And t communcatesa sh n the poducton o mmune potens, all owhch combne to educe nammaton and to thenceate an envonment o epa. Ts s mpotantto autommune dsodes lke nammato boweldsease (as shown), but should equall appl toneuodeeneatve dsease, chonc athts, allees,asthma, and cetanl autsm spectum dsodes.

As we have mentoned, MSC unqueldowneulate nammaton n most aeas o thebod, ncludn the ban. Te ma be ou besthope o epan nammato chanes n the

ut due to Cohns dsease, ulceatve colts, andautstc enteocolts. But moe mpotantl thanthat, as descbed eale, the hold the potental oeulatn nammaton n the ban. reseach ommultple scleoss ndcates that MSC ma acltateepa n the ban whch s tul extaodna.

self OR DONOR sTem Cells?i ou want to ollow stem cell eseach andteatment models, oull need to lean the deencebetween autoloous (sel-donated) vesus alloenec(donated om anothe peson o pesons) cells.Stanel enouh, adpose-deved stem cells ae

mmunolocall pvleed, and, even when thecome om someone else, the eneall lve and donot ht the new enetcall deent host thend themselves cohabtatn wth.

Te medcal lteatue suppots ethe alloeneco sel-donated MSCs (at o bone maow- devedstem cells) n the teatment o nammato boweldsease (iBD). Common teatments o iBD seekto suppess the mmune sstem, and wth that comesa lon lst o seous potental complcatons evendeath om nectons.

But MSC wok d eentl. Te talk to thedeaned mmune sstem to ve t new nstuctons.Te esult s a moe tuned and balanced mmunedeense n the gi tact wthout the danesntnsc n man o the suppessve medcatons. Mpeeence s o the use o autoloous (sel-donated)stem cells. Alloenec stem cells ceate a chmec(2-n-1) elatonshp wth the bod. Ts means thedonos enetcall deent cells cohabtate wththe host despte ben msmatched. Te do not etejected no do the ntate attacks on t he host. Telon-tem eects o chmesms ae had to antcpateat ths tme (althouh eal obsevatons thus a looksae). Fom m pespectve, sel-donated stem cellsae the local choce.

uncton and one that i thnk s moe lkel wheethe wll nd the useulness n autsm. MSCare capable o downregulating chronicinfammation(Fue 6). Tat ets m attentonbecause t s the closest we ma be able to come tothat eset button ive been lookn o. i beleveths s whee stem cells wll nd the place n autsmmmunotheap.

ImmUNe ReGUlATION by mCs(fAT-DeRIveD sTem Cells)Once actvated, MSC seek out aeas on thebod expessn nammaton. it s the natual

poammn. Tnk about an cut ou have had.intall t s swollen and ed and panul. Tat sthe ntal nammato deense eacton that wlldeend the bod om necton and stat to clean

figur 7: In te grapi, te re ox represents te mSc tat isownregulating infaation. ro: Sing et al. Ste ells as potential

terapeuti targets or infaatory owel isease.Front Bioci (schol ed). 2010;2:993-1008.

MSC unquel downeulate nammaton n most aeas o the bod, ncludn the ban. Te mabe ou best hope o epan nammato chanes n the ut due to Cohns dsease, ulceatve colts,and autstc enteocolts. But moe mpotantl than that, as descbed eale, the hold the potental o

eulatn nammaton n the ban.



As we can see n the abstact above, doctosalead have stem cell t heap taeted o iBD.Obvousl, not all chlden wth autsm sue omchonc nammaton, but o those who do, MSCtansplantaton ma epesent a easonable teatmentopton when compaed to iVig o the lon-tem useo ant-nammato medcatons. Ts also meanspatent selecton s mpotant. And adult-deved MSCcan be sel-donated, meann we dont need embos,etuses, o even stanes to povde them o us. just tobe clea the tem adult doesnt mean 18 o 21 eaolds. it ust means cells that ae deved om a p esonan tme ae bth. Ts dstnushes them omembos and etuses, nethe o whch could be usedn autsm apat om an FDA appoved new du t al(whch i do not expect to see n the next decade).

pRODUCING mUlTIpOTeNTIAlADIpOse-DeRIveD sTem CellsTe pctue o the two lass tubes Fue 8 showsthe md-phase o stem cell solaton om adposehuman at. Ts was the esult ae i put the cellsthouh seveal washes, hh speed sepaatontechnques, at deston, pucaton, and solaton.

B ths pont i was ettn close to m oal:bolocal old vable multp otental stem cells. ittook me about 2 hous n the lab to et to that stae ostem cell extacton, and the pocess was a om ove.

At the bottom o the centue tubes a e edblood cells, mmune cells and seveal mllon adpose-deved mesenchmal o multpotental stem cells.Tese pzed stem cells shown below wll be washedseveal moe tmes and o thouh seveal ccles ocentuaton to uthe solate them om othe cells.

NOw ThAT yOU mAy wANTThem CAN yOU GeT Them INThe UNITeD sTATes?yes. i have been taned wth adpose-deved stemcells, and ths technque povdes a stahtowadpocess we can cuentl use n the US. Tee ae,o couse, estctons placed b the FDA on thspocess. Some eneal and undestandable eulatoconcepts o ths pocess ae what we ca ll the 3sames: same peson, same buldn, and same da.

Add to ths anothe set o ules that come omthe Publc Health and Saet Act, s ecton 361:stem cells cannot be hhl manpulated o usedo puposes othe than the nomal uncton. Noone eall undestands what that means n the eal

wold. But t seems clea that ou cannot alte thecells wth lase stmulaton cuentl ben done nothe places, ou cannot add chemcal tas to themto et them to behave n a specal wa cuentlhappenn n nvestatonal du tals, ou cannocultue them to ncease the numbe ths s v eweas a manpulaton, when ou stat the pocess ouhave to nsh n a elatvel shot tme ame a ew

hous. Othewse, the FDA ma assume the ntens cultun o amplcaton o the cell numbes. Talso means banked stem cells ncludn a chlds owcod blood cannot be used.

Whle we ae clea that sel-donated and notsubstantall manpulated stems cells meet theeulato ctea, t s not clea to me whethethese udelnes exclude dono adult stem cells. inothe wods wth pecautons aanst tansmssbldseases could a paent donate stem cells to thechld? At ths pont, the best answe that i can ve

ou s pobabl. Tnk about t ths wa: have oueve donated blood? i so, ou donated cells o

someone else to use n the bod. Tat s al loenecs also totall pemssble. Besdes, whole blood alwcontans a ew stem cells. Obvousl, blood banks to eat lenths to ensue that the blood poductsthe povde ae both sae and clean. in ou iCDrCcentes both n Calona and Floda we outneuse iVig a concentated om o antbodes de vom human blood donos. it comes om hundeo people pe dose, and n the last 15 eas o m usiVig thee have been no contamnatons. Te bloodonaton analo makes me thnk a popel sceendono could povde stem cells the othe udeln

wee also met.Sel-donated adpose tssue at contans lae

numbes o MSC. All we need to do s havest 20-5ml o at less than 5 tablespoons and lbeate thestem cells that ae tapped wthn t. Tat pocess habeen peected ove the last ew eas and s n useon a dal bass n plastc sue centes all ove thecount. Plastc sueons use stem cells to enhancethe successul an o at tanses o cosmetcenhancements. Wthout stem cells, between 70-90o the at tanseed wll de and be eabsobed. Wat-deved stem cells, those numbes ae evesed.

So obvousl, o the oup o cosmetc sueons

alead don stem cell tansplantatons, the ules

Obvousl, not all chlden wth autsm sue om choncnammaton, but o those who do, MSC tansplantaton ma

epesent a easonable teatment opton when compaed to iVig othe lon-tem use o ant-nammato medcatons.

figur 8:

Ste ells in te test tue



pemt an ndvdual havn the own bone maowo at havested, and the stem cells solated and then

e-mplanted.Pepan the stem cells om the lpoaspate s a

tme-consumn and pecse pocess. in Fue 9, we aeabout halwa thouh the 2-3 hou lon pocedue tosael extact the cells wthout hamn them.

i used a ve bus plastc sue cente to leanabout both stem cell havestn lpoaspatonand stem cell solaton and pepaaton Fue9. Havestn nvolves entl aspatn a small

volume o at. Ts s absolutel NO lposucton.Lposucton damaes stem cells and s not sutableo the knd o esults we dese. Lpoaspaton usesa ve small 3mm blunt cannula and a sne not

a vacuum tube. Fo anone amla wth necolo,lpoaspaton s smla to an endometal bops, andlposucton s lke a D&C. Lpoaspaton s a sae andsmple pocedue that can be a ccomplshed n anclean oom n a doctos ofce. it does not eque anopeatn oom, eneal anesthesa, o even sedatonalthouh i assume ou autstc kds wll need somecalmn aent to et them thouh the pocess.

Te total pocess eques popel pepan thepatent o stem cell havest to ncease the eld oactve stem cells. it also eques takn cae o oustem cell tansplants n much the same wa a ametends hs cop ae plantn.

A sImple wAy TO lOOK AT IT:GeTTING sTARTeD wITh sTem Cellsi have been thnkn a lot about stem cells o seveal

eas, watn o the scence to matue to the pontwhee cost-eectve teatment plans stat to makesense. to thnk o stem cells the wa a ame oadene thnks about hs seeds. you ma not know alot about amn, but im condent we all undestandthe concept o plantn seeds to ow owes o

veetables.Eve expeenced adene o ame knows

what the need to do ae bun the seeds. So do

ou: pepae the sol whee ou want to plant seeds.As ou mht expect, t s even moe complex wthautoloous sel-donated MSC stem cells. Fst o all,

we et the seeds stem cells om the ve sol thepesons ecosstem whee we want to eplant them.Tat ecosstem alead ceated poblems. Ou oals to coect those poblems not add to them. Wththe ht plan, we can mpove ou chances o a oodhavest om ou stem cell seeds.

in that toubled ecosstem the exposues whchceated the medcal poblems les the potental oa bad cop and a havest one won. No one would

want to t to ow a cop om dseased seeds. So,how can we make ths wok? Equall no decentame o adene would plant hs seeds n posonedsol. Ou stem cell seeds need deal condtons bothbeoe and ae tansplantn we a e to expect thebest esults.

Based on m eas o expeence and wok wthnutton and toxcolo, i have some suestonsto help ths ente pocess. it makes sense to st othouh some mpotant detoxcaton measuespo to collectn the MSCseeds. Ts wll meansomethn a lttle deent o eve patent based onthe toxc exposue pattens and ndvdua l poblems.

Ten, ollown ou adenn analo, we wllneed to: etlze, wate, povde esh a and sunlht,and keep the weeds out o ou new cop. What doesall ths adenn talk mean n eal-wold tems oepa and eeneaton o someones health?

Fetlzn means vn all o the pope nutentsthe MSCseedseque. Waten means povdnood cculaton blood to the aeas we needto estoe. Fesh a means the ht amount ooxen. Ts s lkel om hpebac oxen theap

[HBO] delveed at the ht tme ae themplanted MSCseedsae statn to ow. One thnthat seems clea s that hpebac oxen nceasesboth stem cell elds t s used po to havestn,and t also nceases the clncal mpact avoabl whenappled ae stem cell mplantaton.

Sunlht apples to the vtamn D [D-3] themmune sstem wll need to help ude the seeds nthe ht decton. Keepn the weeds out natuallmeans peventn oxdatve damae and toxc

exposue to ou valuable MSC cop.i we do all o that ht, we should have clean MSC

seedsto plant and an abundant cop o mmune-manan stem cells wokn to eulate and estoemmune health and balance, whle eston unctonto the ndvdual.

ThIs Is GOOD exAmple Of ANINTeGRATIve AppROACh TO sTemCell TheRApIes fROm OhIO sTATeUNIveRsITy: AND IT Is As seRIOUsAs A heART ATTACKTese eseaches cleal know how best to tend the

stem cell aden.

hrbaric ognationnanc tranantd c gratand unctiona rcor in tinarct artMahmood Khan*, Sarah Meduru, IyyapuK. Mohan, M. Lakshmi Kuppusamy,Sheik Wisel, Aditi Kulkarni, BrianK. Rivera, Robert L. Hamlin1, andPeriannan Kuppusamy

Davis Heart and Lung Research Institute,Division of Cardiovascular Medicine,

Department of Internal Medicine, TeOhio State University, Columbus, OH43210, USA

J Mol Cell Cardiol. 2009 Auust ; 472: 275-287.Te moe i stud ths pape, the moe i espect

the thouht that went nto t. On m blo ive beenpostn a lot about adpose at deved stem cells one tpe o mesenchmal stem cell lne MSC. Tspape ocuses on bone maow-deved stem cells.

Wthout on nto a lenth dscusson about ths,the medcal lteatue documents that adpose andbone maow stem cells both have smla potentalsas stem cells. Ts pape nvestates mce, but nthe human applcaton o ths technolo, adpose-deved MSC ae a moe abundant and pactcal tomanae.

gven the devastatn eects o heat attacks, aswell as how common the ae, ndn bette wasto teat these patents s ctcall mpotant. in anutshell, what these eseaches dd was to ceate heatattacks n mce. Ten they studied the naturaluntreated course, but they also contrastedthat with hyperbaric oxygen (HBO) therapyversus stem cells (MSC), and then combinedMSC + HBO. Te pctues n Fue 10 speak o

figur 9: dr. brastreet in teste ell laoratory isolating

mSc ro lipoaspirate.

One thn that seems clea sthat hpebac oxen nceasesboth stem cell elds t s usedpo to havestn, and t alsonceases the clncal mpactavoabl when appled ae

stem cell mplantaton.


8/8

themselves. Te puple aeas epesent lvn heattssue and the pale blue aeas ae sca tssue. i wllexplan the sncance o the pctues n Fue 10above.

just b lookn at the colos t s clea that MSC+ HBO s a bette than even stem cells povdedalone. Ts tpe o stud s exactl what we needn the eld o nteatve medcne. i have beencomplann about clncal studes that contol oonl one vaable. i undestand the scentc easons,

figur 10: Te eet o mSc + hbO on ouse eart ater inartion.

but n ealt that ust snt the wa complex bolocalsstems wok.

Tis study is just awesome. Lets look atFigure 11 below and get into some more details.

i added the dotted lnes to help n m dscusson.As a pme n cadolo, the actve phase o heatmuscle wok s done dun rELAXAiON othe heat muscle. Tat s whee neal all o theene s consumed, because heat muscle has toactvel echae o the next beat. Tnk about

a ubbe band ou want to shoot.you pull t back and that equesene. Ten ou let t and thattook ve lttle eot. Te ed dottedlne n Fue 11 s set at the ejectonacton (EF) (measued n pecentaeponts). in smple tems, that meanswhat pecent o blood leaves theheat on an ven heat beat (olettn the ubbe band ). Mceae bette at ths than we ae. Teae appoxmatel 90% efcent wtheach heat beat: we humans aveae

55-70%. Te blue lne epesentsactonal shotenn, whch measuesand atos the chane n the damete

o the le ventcle (man pumpn chambe)between the contacted and elaxed states. in thsmouse stud, the heat attack educed the EF to60% (a 1/3 educton). Hpebac oxen plus stcells etuned the ejecton acton to about 75%HBO b tsel made a manal, but not sncanncease n the EF. Stem cells b themselves (theMSC alone poton o the stud) dd help, butsncantl less than the combnaton o MSC aHBO.

Ts stud s smpl amazn scence. it teachesus the speccs about stem cell mplantn and whHBO s a valuable adjunct to the pocess. But mompotantl, t teaches us to use nteated theapto optmze outcomes.

For me it seems very clear: stem cells aregood, but stem cells plus HBO is signifcanbetter. In other words, integrative medicinmeans being a better gardener.

i hope m analo helps these concepts to beeasl emembeed. Fom m obsevatons o thestem cell ndust thus a, t seems that a lot opeople want to peom stem cell mplantn, but

ew seem to want to tend to the aden. Pleasekeep ths vtal analo and concept n mnd as ouconsde stem cell optons o ou health o that ou loved ones.

fINAlly . . .Even beoe we stat on ths joune t s ctcalto select patents who would seem to be the bestcanddates o stem cell theap.

We ae stll leann about patent selecton,howeve, i beleve m pevous wok on bomakecan be used to help us wth the selecton pocess. Tull atcle s pesented hee:http://www.thorn

com/altmedrev/.ulltext/15/1/15.pd.Aan, i talk about new eseach wth stem

cells extensvel on m stewww.drbradstreet.org. Please check out those dscussons o moenomaton.

1. Gupta S, Aggarwal S, Heads C. Dysregulatedimmune system in children with autism: beneficialeffects of intravenous immune globulin on autisticcharacteristics. J Autism Dev Disord. 1996Aug;26(4):439-52.

2. Stubbs EG., Autistic children exhibitundetectable hemagglutination-inhibitionantibody titers despite previous rubellavaccination. J Autism Child Schizophr. 1976Sep;6(3):269-74.

3. Chauhan A, Chauhan V, Brown T (Eds.).(2010).Autism: Oxidative Stress, Inflammation,and Immune Abnormalities. Boca Raton, FL: CRCPress.

4. Crocker SJ, Bajpai R, Moore CS, Frausto RF,Brown GD, Pagarigan RR, Whitton JL, Terskikh AVIntravenous administration of Human ES- derivedNeural Precursor Cells Attenuates Cuprizone-induced CNS Demyelination. Neuropathol ApplNeurobiol. 2011 Jan 28. [Epub ahead of print]

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Stem Cells: Real Possibilities in Autism? by James Jeffrey Bradstreet, MD