Stem Cells

Max Johnson

Manhasset Science Research

Need

http://www.nature.com/nrd/journal/v1/n2/box/nrd742_BX1.html

Figure 1

Knowledge Base

http://www.kumc.edu/stemcell/early.html

SCNT-Somatic Cell Nuclear Transfer

Figure 2

Knowledge Base 2

http://www.kumc.edu/stemcell/early.html

How to culture stem cells

Figure 3

Knowledge base 3

http://www.sciencelearn.org.nz/contexts/you_me_and_uv/sci_media/images/the_electromagnetic_spectrum

The electromagnetic spectrumMost harmful UV ray

Figure 4

Literature Review

George Q. Daley’s stem cell procedure

http://www.nature.com/nbt/journal/v23/n7/fig_tab/nbt0705-826_F1.html

Figure 5

Literature Review 2

Israeli scientists results

http://www.israel21c.org/bin/en.jsp?enDispWho=Articles%5El2394&enPage=BlankPage&enDisplay=view&enDispWhat=object&enVersion=0&enZone=Health

Figure 6

Literature Review 3

Michael D Jones

how stem cells are made

http://en.wikipedia.org/wiki/File:Stem_cells_diagram.pngNote: I haven’t found his original work but this is an example of what he did

Figure 7

Alternate Hypothesis-The mutated planaria will take more time to regenerate than the non-mutated planaria.

Hypothesis

In 1990, 35 to 40 billion dollars were spent in cancer care (International Society of Technology Assessment in Health Care). With research stem cells have been found in adult bodies and by reprogramming these embryonic stem cells scientists can regenerate human tissues by putting them in drugs.

Purpose

Methodology20 Planaria (n=3)

10 regular10 mutated

Planaria are sliced in half

Data Analysis

2 weeks later

2 weeks for regeneration with UV radiation for 2 minutes

Protocols for measurement

• The Planaria culture (10 per culture) will eat a diet of 20 grams of liver a week

• During the second regeneration (with the group with mutations) the measured amount will be amount of UV radiation (time they have been subjected to the chamber) compared to the time it takes to regenerate (in days)

Do-Ability

• Cheap (only planaria)

• Easy

• We already have the most expensive item (A Ultra Violate Ray chamber)

• Applies to life

BudgetVendor Item number Description/

Name of item

Quantity Unit price Total price

Manhasset High School Science Lab

N.A. Ultra Violate ray chamber

1 X X

Local Manhasset Grocery store

N.A. Liver 5 (1 lb) X X

Sargent-Welch 2009

WL50506 Black Planaria 1 (25-30) $9.99 $9.99

Bibliography• American Pregnancy Association. “In Vitro Fertilization.” IVF 05/2007. May 8, 2009. <

http://www.americanpregnancy.org/infertility/ivf.html >.• American Society of Hematology. "Blood Cells Can Be Reprogrammed To Act As Embryonic

Stem Cells." ScienceDaily 21 April 2009. 22 April 2009 <http://www.sciencedaily.com /releases/2009/04/090420142421.htm>.

• Birch, Nick. "Flatworm helps researchers study stem cells.” September 17 2008. Cambridge UK and Bio-Medicine. 17 Sept. 2008. Bio-Medicine, Cambridge UK. 18 May 2009 <http://www.bio-medicine.org/biology-news-1/Flatworm-helps-researchers-study-stem-cells-and-cancer-4921-1/>.

• Franklin J, Franzini L, Williams A; International Society of Technology Assessment in Health Care. “Can we evaluate the outcomes of cancer treatment?” Cancer research 1994. May 7, 2009. http://gateway.nlm.nih.gov/MeetingAbstracts/ma?f=102211786.html.

• University of Oklahoma. "Cancer Stem Cells Isolated: Could Lead To New Drugs To Stop Cancer From Returning." ScienceDaily 12 September 2008. 4 May 2009 <http://www.sciencedaily.com /releases/2008/09/080911140813.htm>.

• University of Kansas Medical Center. “Stem Cell Research Basics: Early Stem Cells.” Stem cells May 25, 2005. May 7, 2009. http://www.kumc.edu/stemcell/early.html

• U.S. Department of Health and Human Services, 2009. “Stem cell basics.” Stemcells 28 April 2009. May 4, 2009. http://stemcells.nih.gov/

• U.S. National Library of Medicine. “Medical Encyclopedia.” Stem Cell Research. U.S. National Library of Medicine. <http://www.nlm.nih.gov/medlineplus/ency/article/007120.htm>. 4 May 2009.

• U.S. National Library of Medicine U.S. National. “PTEN.” PTEN May 15, 2009. May 19, 2009. http://ghr.nlm.nih.gov/gene=pten