Supporting Information for Applied Biochemistry and Biotechnology

Bioremediation of Petrochemical Wastewater Containing BTEX

Compounds by Immobilized a New Bacterium Comamonas sp. JB in

Magnetic Gellan Gum

Bei Jiang *, Zunchun Zhou, Ying Dong, Bai Wang, Jingwei Jiang, Xiaoyan Guan,

Shan Gao, Aifu Yang, Zhong Chen, Hongjuan Sun

B. Jiang () · Z.-D. Chun · Y. Dong ·B. Wang · J.-W. Jiang · X. -Y. Guan · S. Gao ·A.-F. Yang ·

Z. Chen ·H.-J. S

Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science

Research Institute, Dalian, 116023, China

e-mail: jiangbei1983@163.com

Table S1 The wastewater quality of petrochemical wastewater

Wastewater qualityPhenol

(mg L-1)

Benzene

(mg L-1)

Toluene

(mg L-1)

Ethylbenzene

(mg L-1)

o-xylene

(mg L-1)

m-

xylene

(mg L-1)

p-xylene

(mg L-1)

NH3-N

(mg L-1)

Raw wastewater 100 8.5 10.5 7.2 3.5 5.5 5.5 30

High phenol- and BTEX-concentrated petrochemical wastewater 100 30 30 30 30 30 30 15

Figure Legends

Fig. S1 SEM images of Comamonas sp. JB immobilized in nonmagnetically immobilized cells

(a), a-Fe2O3-magnetically immobilized cells (b), Fe3O4-magnetically immobilized cells (c), and r-

Fe2O3-magnetically immobilized cells (d), and r-Fe2O3-magnetically immobilized cells after eight

cycles of degradation experiments (e).

Fig. S2. Reuse of magnetically (r-Fe2O3, a) and nonmagnetically immobilized cells (b) coupling

with activated zeolite for the degradation of petrochemical wastewater containing phenol (■),

benzene (■), toluene (■), ethylbenzene (■), o-xylene (■), m/p-xylene (■) and NH3-N (■).

Fig. S3 Reuse of r-Fe2O3-magnetically immobilized cells coupling with activation zeolite for

phenol (a), benzene (b), toluene (c), ethylbenzene (d), o-xylene (e), m/p-xylene (f) degradation at

time course. ■: first cycle; ●: second cycle; ▲: third cycle; ▼: fourth cycle; ◆: fifth cycle; ◄:

sixth cycle; ►: seventh cycle; ★: eighth cycle.

Fig. S4 Reuse of nonmagnetically immobilized cells coupling with activation zeolite for phenol

(a), benzene (b), toluene (c), ethylbenzene (d), o-xylene (e), m/p-xylene (f) degradation at time

course. □: first cycle; ○: second cycle; △: third cycle; ▽: fourth cycle; ◇: fifth cycle; ◄: sixth

cycle; ►: seventh cycle;☆: eighth cycle.

Fig. S5 The concentration of stain JB in nonmagnetically and magnetically immobilized cells by

qPCR assays. Sample 1 to 4: the concentration of stain JB in nonmagnetically, a-Fe2O3, Fe3O4 and

r-Fe2O3 immobilized cells after first cycle of degradation experiment, respectively; Sample 5 to 6:

the concentration of stain JB in nonmagnetically and r-Fe2O3 immobilized cells after eighth cycle

of degradation experiment, respectively.

(a) (b) (c)

(d) (e)

Fig. S1

(a)

(b)

Fig. S2

(a) (b)

(c) (d)

(e) (f)

Fig. S3

(a) (b)

(c) (d)

(e) (f)

Fig. S4

Fig. S5