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Rapid Biosensor for the Detection of Mycotoxin in Wheat
• Start date: September 2010• Duration: 36 months• Website: www.mycohunt.eu• Funding Scheme: FP7 Research
for the Benefit of SME-AGs
The research leading to these results has received funding from the European Community’s Seventh Framework Programme (FP7/2007-2013) under grant agreement n° 286522.
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PROJECT PARTNERSORGANIZATION NAME COUNTRY
Hungarian Grain and Feed AssociationGreek Associations of Traders and Exporters of Cereal By-products and Feeds
Confederacion Espanola de Fabricantes de Alimentos Compuestos para Animales
Associación Espanola de Fabricantes de Masas CongeladasSynagraImpulsOSVDunagabonaDimitriakiHogeschool GhentUniversiteit GhentUnion of Agricultural Cooperatives of RethymoEvaluation technologique, Ingénierie et ApplicationsBIOFORUMEST
HungaryGreeceSpainSpainBelgiumPolandItalyHungaryGreeceBelgiumBelgiumGreeceFranceGreeceUK
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PROJECT IDEA
TECHNOLOGICAL OBJECTIVES• To develop effective, non-destructive sampling apparatus in order to assure adequately representative
measurement aiming at 90% correlation with the bulk samples.• To assess detection limit of 50 ppb, efficacy, specificity and sensitivity of the sensor• To develop a data evaluation algorithms and to set up correlation databases• To develop the ‘plug & play’ disposable cartridge to house the antibody-binding assaySCIENTIFIC OBJECTIVES• The understanding and determination of parameters (temperature, pressure, vacuum, etc) affecting the sampling
precision avoiding the damaging of grain.• To develop immobilising methods for sensitive antibodies, and assess different approaches to enhance sensitivity,
reproducibility, life-time, detection range, detection limit, and/or other specifications.• The investigation of the cross-reactivity of other trichothecenes such as 3-acetyl-DON, 15-acetyl-DON, nivalenol,
etc.ECONOMIC OBJECTIVE• To realize a commercial price for the sensor while keeping the price of the whole apparatus low.TRAINING, DISSEMINATION AND EXPLOITATION OBJECTIVES• The development of training activities addressed to the SME members of the SME-AGs, first in pre-validation stage
in order to ensure the understanding of the technology operation before the partners initiate the validation tasks.• Technology transfer will be carried out to present the project results.• To develop a sound dissemination plan to deliver at least 4 workshops, presenting the results of the project in
European conferences and specialised magazines
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PROJECT IDEA
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RESULTS SO FAR
• The MYCOHUNT system was fully designed reflecting the content of the system specification with the assistance of SME Association and SME partners.
• The MYCOHUNT concept of testing DON from wheat dust has been proven via defining the correlation between DON found in wheat grain samples and in wheat dust samples.
• DON selective antibodies have been developed to be used in the immunosensor for DON detection. Assay development and characterization for the immunosensor is under progress.
• The components of the prototype have been constructed. The physical realization and the integration of the complete system including the sampling unit, the sensor unit and their control system are under development and will be installed at the end-user facilities to continue with the prototype demonstration.
