1/22/2015 SR0183, Bolton Broth Selective Supplement | Oxoid Product Detail

http://www.oxoid.com/uk/blue/prod_detail/prod_detail.asp?pr=SR0183&org=154&c=uk&lang=EN 1/2

Corporate (UK)

Productsby Organism

Productsby Category

ProductList

Safety DataSheets

TechnicalSupport

LatestNews

Exhibitions

QualityCertificates

Literature

BusinessContinuity

Material Safety Data Sheet

Click here to downloadMaterial Safety Data Sheetsfor this product.

Further Information

Click here to view moreinformation related to thisproduct.

OrganismsOrganisms this productworks with:Campylobacter

For this OrganismOther products used in theisolation of Campylobacter:AG0025AnaeroJarCN0020CampyGen CompactCN0025CampyGen 2.5 litreCN0035CampyGen 3.5 litreCM0271Blood Agar Base No 2CM0689Campylobacter Agar BaseCM0739Blood Free CampylobacterSelective Agar BaseCM0935Campylobacter Agar Base(Karmali)CM0983Bolton BrothSR0048Laked Horse BloodSR0069Campylobacter SelectiveSupplement (Skirrow)SR0085Campylobacter SelectiveSupplement (Butzler)SR0098Campylobacter SelectiveSupplement (BlaserWang)SR0117Preston CampylobacterSelective SupplementSR0155CCDA SelectiveSupplement.SR0167Campylobacter SelectiveSupplement (Karmali)SR0174C.A.T. Supplement

Culture Media SupplementsBOLTON SELECTIVE ENRICHMENT BROTH

BOLTON BROTH

CODE: CM0983

A medium for the selective preenrichment of Campylobacter organisms in food samples.

Typical Formula* gm/litreMeat peptone 10.0Lactalbumin hydrolysate 5.0Yeast Extract 5.0Sodium chloride 5.0Alphaketoglutaric acid 1.0Sodium pyruvate 0.5Sodium metabisulphite 0.5Sodium carbonate 0.6Haemin 0.01pH 7.4 ± 0.2 @ 25°C

* Adjusted as required to meet performance standards

BOLTON BROTH SELECTIVE SUPPLEMENT

CODE: SR0183

Vial contents (each vial is sufficient for 500ml of medium) per vial per litreCefoperazone 10.0mg 20.0mgVancomycin 10.0mg 20.0mgTrimethoprim. 10.0mg 20.0mgCycloheximide 25.0mg 50.0mgDirectionsAdd 13.8g of Bolton Broth to 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes.Cool to 50°C. Aseptically add 25ml Laked Horse Blood SR0048 and 1 vial of Bolton Broth SelectiveSupplement SR0183, reconstituted as directed. Mix well and distribute into sterile screw top containers.

DescriptionBolton Selective Enrichment Broth is intended for the preenrichment of Campylobacter in food samples.Campylobacter are Gramnegative, spirally shaped microaerophilic organisms which may be present inraw milk, untreated water, improperly handled food and undercooked meats, poultry and shellfish.Human consumption of these organisms can result in a range of clinical illnesses from transientasymptomatic colonisation to severe dysentery. The symptoms of Campylobacter enteritis includediarrhoea, stomach pain, nausea, fever, headache and muscle pain. Complications of infection byCampylobacter jejuni may include unnecessary appendectomies as a result of abdominal pain, reactivearthritis or GuillianBarré syndrome1. Campylobacter infection is recognised as one of the most commoncauses of bacterial gastroenteritis in humans, and the minimum infective dose may be as low as 500800cells1.

Since awareness of the apparent role of Campylobacter in human disease was heightened by Skirrow in19772, a great number of culture media have evolved in response to the need to optimise performance.There was early recognition of the need for enrichment culture when examining food samples toovercome the damaging effects that food processing and preservation techniques can have onCampylobacter cells. Use of lower incubation temperatures in the early stages of enrichment is nowwidely established as an aid to cell recovery3. This principle was employed by Bolton in the developmentof his enrichment broth4.

Campylobacter can be injured by food processing and preservation procedures3. This makes themsusceptible to selective agents which are tolerated by undamaged cells. False negative results areavoided through use of recovery medium such as Bolton Selective Enrichment Broth which increases thenumber of cells available for culture, first by resuscitating injured organisms and then encouraging them

Part of Thermo Fisher Scientific

Home | Contacts | Links | Quality Standards | Instructions for Use | Careers

1/22/2015 SR0183, Bolton Broth Selective Supplement | Oxoid Product Detail

http://www.oxoid.com/uk/blue/prod_detail/prod_detail.asp?pr=SR0183&org=154&c=uk&lang=EN 2/2

SR0183Bolton Broth SelectiveSupplementSR0204Modified PrestonCampylobacter SelectiveSupplementSR0205Modified Karmali SelectiveSupplementSR0208Modified Bolton BrothSelective SupplementSR0232Campylobacter GrowthSupplement (Liquid)LT0504Foodborne Pathogens,Monograph Number 3 CampylobacterDR0150Campylobacter Test KitQB0003DuPont QUALICON BAX®SYSTEM Q7QB3449BAX® CAMPYLOBACTERJEJUNI/COLI/LARI KIT (96tests)PB0118CAMPYLOBACTERSELECTIVE AGAR(SKIRROW)PB0295CAMPYLOBACTERSELECTIVE AGAR(PRESTON)PO0839CAMPYLOBACTER CATAGARPO1185Brilliance CampyCountAGAR

to multiply.Bolton Selective Enrichment Broth contains nutrients to aid resuscitation of sublethally injured cells, andis formulated to avoid the need for a microaerobic atmosphere. Initial incubation is carried out at 3037°C, depending on the type of food to be examined. After the preenrichment, the incubationtemperature is raised to 42°C to increase the selective pressures on competing organisms.

Inclusion of sodium metabisulphite and sodium pyruvate in Bolton Broth quenches toxic compounds thatmay form in the culture medium. These additions also increase the aerotolerance of the culture. Theantibiotics contained in Bolton Broth Selective Supplement SR0183 optimise selectivity forCampylobacter spp. Vancomycin active against Gram positives. Cefoperazone predominantly activeagainst Gram negatives. Trimethoprim active against a wide variety of Gramnegative and Grampositive organisms. Cycloheximide active against yeasts.

TechniqueOne method of use is as follows:Place 25g of food sample in 225ml Bolton Selective Enrichment Broth (prepared as described above)and homogenise the mixture using a Stomacher (or similar device). Bolton Selective Enrichment Brothdoes not require incubation in a microaerobic environment, but must be used in screw topped containerswhich are filled to within 20mm of the top4. Incubate for 4 hours at 37°C, followed by further incubationat 42°C . The broth can be subcultured after 24 hours and 48 hours on to either Modified CCDA(CM0739 + SR0155) or Preston Agar (CM0689 + SR0117 + SR0048)4.For other methods please refer to BAM5.

Storage conditions and Shelf LifeStore the dehydrated medium at 1030°C and use before the expiry date on the label.Store the prepared medium at 28°C for up to 2 weeks.

AppearanceDehydrated medium: Straw coloured, freeflowing powderPrepared medium: Broth: Straw coloured solution containing small black particles

Quality control

Positive control: Expected results: when subcultured on modified CCDACampylobacter jejuni ATCC® 29428* Good growth; grey coloured coloniesNegative control: Escherichia coli ATCC® 25922* No growth

* This organism is available as a CultiLoop®.

References1. National Advisory Committee on Microbiological Criteria for Foods (1994). Journal of Food Protection57 (12): 11011121.2. Skirrow, M.B. (1977). British Medical Journal 2: 911.3. Post, D. E. (1995). FoodBorne Pathogens Monograph Number 3 Campylobacter .4. Bolton, F.J. (1995) Personal communication. 5. Hunt, J.M. (1998) Campylobacter. In: F.D.A. Bacteriological Analytical Manual, 8th Edition (RevisionA) 7.017.27. AOAC, Arlington Va.

©2001 2015 Oxoid Limited, All rights reserved. Copyright, Disclaimer and Privacy Policy | Conditions of Sale | About Us | Cookies

Thermo Fisher Scientific Inc.

Related Searches

Drug Development

Thermo Fisher

Chromatography AndMass Spectrometry

PharmaceuticalResearch

?

allgenius