spektrofotometri uv zat campuran

7/21/2019 spektrofotometri uv zat campuran

1/39

SPEKTROFOTOMETRI UV

MULTIKOMPONEN

PENETAPAN KADAR INH

DAN VITAMIN B6

MAKALAH ANALISIS INSTRUMENTAL

MODUL IV

SPEKTROFOTOMETRI UV MULTIKOMPONEN

PENETAPAN KADAR INH DAN

VITAMIN B6


2/39

Disusun Oleh:

Kelompo : A !An""o#$ : %& E$ Se#i$nis$ 'K %(( %%( (()&

*& E$ P+$sn$ P, 'K %(( %%( (%-&

-& D.$h Ris/$+i Pi#$lo$ S, 'K %(( %%( (-6&

0& El1esi Me1is$ I, 'K %(( %%( (-)&

Ko+e#o+ :

LABORATORIUM ANALISIS INSTRUMENTAL

FAKULTAS FARMASIUNIVERSITAS MUHAMMADI2AH SURAKARTA

*(%*


3/39

ABSTRAK

Me#o1e pe+s$m$$n simul#$n 1il$u$n 1en"$n men"uu+ * $#$u le3ih p$n4$n"

"elom3$n" .$n" m$n$ m$sin"5m$sin" omponen #i1$ $$n men"$n""u, K+omoo+

.$n" 3e+3e1$53e1$ 1$+i #i$p omponen $$n mempun.$i eu$#$n $3so+3s 7$h$.$ .$n"

3e+3e1$ pul$ p$1$ s$#u 1$e+$h p$n4$n" "elom3$n", Pen"ui+$n 1il$u$n p$1$

m$sin"5m$sin" l$+u#$n p$1$ #i$p p$n4$n" "elom3$n" 'menu+u# 3$n.$n.$ omponen&8sehin""$ 1ipe+oleh pe+s$m$$n hu3un"$n $n#$+$ $3so+3$nsi 1en"$n onsen#+$si p$1$

p$n4$n" "elom3$n" #e+se3u#,

PENDAHULUAN

DASAR TEORI

Spe#+oo#ome#+i se+$p $1$l$h pen"uu+$n se+$p$n +$1i$si ele#+om$"ne#i

p$n4$n" "elom3$n" #e+#en#u .$n" smpi#8 men1e$#i mono+om$#i .$n" 1ise+$p 9$#,

Pen"uu+$n se+$p$n 1$p$# 1il$u$n p$1$ 1$e+$h ul#+$iole# ' %;( < -)( nm& $#$u


4/39

p$1$ 1$e+$h 7$h$.$ #$mp$ ' -)( < =)( nm&, D$.$ se+$p $#$u se+$p$n 4enis 9$# p$1$

3$#$s53$#$s $1$+ #e+#en#u $1$l$h #e#$p8 #i1$ #e+"$n#un" 1$+i in#ensi#$s +$1i$si8 p$n4$n"

4$l$n sin$+ 1$n $1$+ l$+u#$n8 sehin""$ spe#+oo#ome#+i se+$p 1$p$# 1i"un$$n un#u

pene#$p$n $1$+, Al$# spe#+oo#ome#e+ p$1$ 1$s$+n.$ #e+1i+i $#$s sum3e+ sin$+ mono+om$#o+8

#emp$# sel un#u 9$# .$n" 1ipe+is$8 1e#e7#o+8 pen"u$# $+us8 1$n $l$# uu+ $#$u pen7$#$#,

Spe#+oo#ome#e+ 1$p$# 3ee+4$ se7$+$ o#om$#i $#$upun #i1$8 1$p$# mempun.$i s.s#em

sin$+ #un""$l $#$u "$n1$,

'Anonim8 %;=;&

B$"i$n moleul .$n" men"$3so+3si 1$l$m 1$e+$h ul#+$iole# 1$n 1$e+$h sin$+ #$mp$

1in.$#$$n se3$"$i +omoo+, D$l$m s$#u moleul 1$p$# 1i$n1un" 3e3e+$p$ +omoo+,

>i$ +omoo+ 1ipis$h$n s$#u s$m$ l$in p$lin" se1ii# oleh 1u$ $#om $+3on 4enuh8 m$$

#i1$ $1$ emun"in$n $1$n.$ on4u"$si $n#$+$ "u"us +omoo+,

'He+m$nn 1$n ?o##+ie18 %;;)&

Aspe Ku$li#$#i

D$#$ spe#+$ UV se7$+$ #e+sen1i+i #i1$ 1$p$# 1i"un$$n un#u i1en#ii$si

u$li#$#i o3$# $#$u me#$3oli#n.$, A$n #e#$pi 1i"$3un" 1en"$n 7$+$ l$in sepe+#i

Spe#+os#opi In+$me+$h8 +eson$nsi m$"ne# in#i8 1$n spe#+os#opi m$ss$8 1$p$# 1i"un$$n

un#u m$su1 i1en#ii$si@$n$lisis u$li#$#i sen.$/$ #e+se3u#,

Aspe Ku$n#i#$#i

D$l$m $spe u$n#i#$#i8 su$#u 3e+$s +$1i$si 1ien$$n p$1$ 7upli$n 'l$+u#$n

s$mpel& 1$n in#ensi#$s sin$+ +$1i$si .$n" 1i#e+us$n 1iuu+ 3es$+n.$,

An$lisis * 7$mpu+$n se7$+$ 3e+s$m$5s$m$

Bil$ 1iin"in$n pen"uu+$n * 3u$h sen.$/$ se7$+$ 3e+s$m$5s$m$ se7$+$

spe#+oo#ome#+i8 m$$ 1$p$# 1il$u$n p$1$ * p$n4$n" "elom3$n" .$n" m$n$ m$sin"5

m$sin" omponen #i1$ s$lin" men""$n""u $#$u "$n""u$n 1$+i omponen .$n" l$in

p$lin" e7il, Du$ 3u$h +omoo+ .$n" 3e+3e1$ $$n mempun.$i eu$#$n $3so+3si

7$h$.$ .$n" 3e+3e1$ pul$ p$1$ s$#u 1$e+$h p$n4$n" "elom3$n", Pen"uu+$n 1il$u$n

p$1$ m$sin"5m$sin" l$+u#$n p$1$ 1u$ p$n4$n" "elom3$n"8 sehin""$ 1ipe+oleh 1u$

pe+s$m$$n hu3un"$n $n#$+$ $3so+3$nsi 1en"$n onsen#+$si p$1$ 1u$ p$n4$n"

"elom3$n"8 $i3$#n.$ onsen#+$si m$sin"5m$sin" omponen 1$p$# 1ihi#un",

'?$n14$+8 *((=&


5/39

TU>UAN

P+$#i$n 1ih$+$p$n m$mpu mene#$p$n $1$+ INH 1$n i#$min B6 1$l$m

s$mpel .$n" men"$n1un" 1u$ omponen $#$u le3ih men""un$$n $l$# spe#+oo#ome#e+

UV 1en"$n me#o1e pe+s$m$$n simul#$n,


6/39

METODOLO?I

ALAT DAN BAHAN

5 Al$#

%, Spe#+oo#ome#e+ UV

*, Kue#-, L$3u #$$+ %( mL

0, Bee+ "l$ss %(( mL

!, Pipe# olume

6, Mi+opipe#

=, P+opipe#

), E+lenme.e+

;, Tim3$n"$n $n$li#i

%(, Pipe# #e#es

5 B$h$n

%, T$3le# .$n" men"$n1un" INH 1$n i#$min B6

*, Au$1es#

-, L$+u#$n 3$u INH (8%

0, L$+u#$n3$u Vi#$min B6 (8%

PROSEDUR RESMI

Vi#$min B6 'Pi+i1oCin Hi1+olo+i1$&

?olon"$n $n$lisis : V

: )H%*NO-l '*(!86&8 #i#i leleh *%(o8 3u3u +is#$l pu#ih8 #$ 3e+3$u8 +$s$ $s$m5p$hi#,


7/39

Kel$+u#$n

1$l$m

$i+ E#$nol $se#on E#e+ lo+oo+m

1 : 8 1 : 100 Tak larut Tak larut Tak larut

Pemeriksaan kualitatif:%& Re$si 3esi 'III& lo+i1$ : /$+n$ me+$h

*& Ke 1$l$m 7$mpu+$n * mL l$+u#$n $s$m sul$nil$# #e+1i$9o#$si 1$l$m % mL - N N$OH

1i#$m3$h i+$(i+$ ! m" 9$#, L$+u#$n 3e+/$+n$ unin" #u$ s$mp$i 4in""$ emu1i$n

1i#$m3$h$n * mL - N $s$m $se#$#8 3e+u3$h men4$1i me+$h,

-& Ke 1$l$m l$+u#$n !( m" 9$# 1$l$m % mL $i+ 1i#$m3$h$n % #e#es l$+u#$n #em3$"$ sul$#

* 1$n % mL - N N$OH8 #e+3en#u /$+n$ 3i+u un"u,

0& Se4uml$h % m" 9$#8 1il$+u#$n 1$l$m %( mL $i+8 e 1$l$m % mL l$+u#$n 1ilo+inon

lo+emi1$ (8(0 1$l$m e#$nol mu#l$ 1$n % #e#es l$+u#$n 6 N $moni$8 #e+3en#u /$+n$

3i+u8 e 1$l$m % mL l$+u#$n l$inn.$8 1i#$m3$h l$+u#$n $s$m 3en9o$#e - % mL

1ilo+inon lo+imi1$ 1$n % #e#es l$+u#$n $moni$8 #i1$ #im3ul /$+n$ 3i+u,

Pemeriksaan kuantitatif:

%& Ti#+$si : L$+u#$n 9$# 1$l$m $s$m $se#$# '1i#$m3$h$n l$+u#$n +$s$ 'II& $se#$#& 1i#i#+$si

1en"$n (8(- N $s$m pe+lo+$# '%@*( mmol& s$mp$i #im3ul /$+n$ 3i+u, In1i$#o+ - #e#es

l$+u#$n n"u K+is#$l,

*& 1$l$m $i+ : **( p$1$ *!0 nm

0*! p$1$ -*0 nm,

Isoni$9i1 'INH&

?olon"$n $n$lisis : IV8 V

: 6H=H-( '%-=8%&8 4$+$ 7$i+ %=( < %=0o8 K+is#$l pu#ih8 +$s$ p$hi#,

Kel$+u#$n

1$l$m

$i+ E#$nol $se#on E#e+ lo+oo+m

1 : 8 1 : 45 1 : 1000 Tak larut 1 : 1000


8/39

Pemeriksaan kualitatif:

%& Isoni$9i1 'INH& me+e1usi l$+u#$n Pe+$ Ni#+$#8 Amoni$8 pe+e$si Fehlin"8 1$n l$+u#$n

K$lium Pe+m$n"$n$# 1in"in,

*& L$+u#$n !( m" 9$# 1i+e$si$n 1en"$n % mL l$+u#$n *80 1i5ni#+olo+o3en9ol '%8( "+@%((

m*e#$nol 1i3u$# se"e+$& 1$n 0 #e#es - N N$OH, D$l$m /$#u sin"$#8 #e+3en#u l$+u#$n

me+$h 7ol$# .$n" #i1$ 3e+u3$h se$lipun 1ien7e+$n 1en"$n $i+,

-& Se4uml$h *( m" 9$# 1$n %(( m" N$#+ium K$+3on$# e+in" 1ip$n$s$n p$1$ $7$

po+selen8 #e+7ium 3$u pi+i1in,

Pemeriksaan kuantitatif:

%& Ti#+$si : L$+u#$n 9$# 1$l$m %) mL As$m Ase#$# 3e3$s $i+ 1$n * mL Anhi1+i1$ Ase#$#

1i#i#+$si 1en"$n (8(! N As$m Pe+lo+$# '%@*( mmol& s$mp$i #im3ul /$+n$ hi4$u, In1i$#o+

% #e#es l$+u#$n un"u +is#$l,*& 1$l$m $i+ : -)( p$1$ *66 nm,

ARA KER>A

%, Menen#u$n @ Men7$+i H$+"$

INH 1$l$m $i+ -)( p$1$ *66 nm

Vi#$min B6 1$l$m $i+ **( p$1$ *!0 nm

0*! p$1$ -*0 nm

*, A, Pene#$p$n L$+u#$n S#o INH (8%

Di#im3$n" %(( m" INH,

Dim$su$n e 1$l$m l$3u #$$+,

Di#$m3$h pel$+u# $i+ $1 %(( mL,

Di3u$# l$+u#$n s#o 3$u INH (8% ,

Nb: sudah tersedia di laboratorium


9/39

B, Pene#$p$n L$+u#$n S#o Vi#$min B6 (8%

Di#im3$n" %(( m" Vi#$min B6,

Di#$m3$h pel$+u# $i+ $1 %(( mL,

Di3u$# l$+u#$n s#o 3$u Vi#$min B6 (8% ,

Nb: sudah tersedia di laboratorium

-, A, Di#en#u$n m$C

Di$m3il %(( L l$+u#$n s#o INH (8% 3@

Dim$su$n e 1$l$m l$3u #$$+ %( mL,

Di#$m3$h pel$+u# $i+ $1 %(8( mL,

Dim$su$n e 1$l$m ue#,

Diuu+ +es$p$nn.$ p$1$ 3e+3$"$i p$n4$n" "elom3$n" un#u menen#u$n m$C,

+$n"e : *(( < 0(( nm,

B, Di$m3il %(( L L$+u#$n S#o Vi#$min B6 (8%


10/39

Dim$su$n 1$l$m l$3u #$$+ %( mL,

Di#$m3$h $u$1es# $1 %( mL,


Diuu+ +es$p$nn.$ p$1$ 3e+3$"$i p$n4$n" "elom3$n" un#u menen#u$n m$C,

0, A, Pen"uu+$n A3so+3$nsiDi$m3il %(( L l$+u#$n s#o INH (8% 3@

Dim$su$n e 1$l$m l$3u #$$+ 1$n 1i#$m3$h$n $i+ $1 %( mL,


Di3$7$ $3so+3$nsin.$ p$1$ se#i$p p$n4$n" "elom3$n" m$C p$1$ INH '*6! nm&

1$n Vi#$min B6 '-*08! nm&,

B, Di$m3il *(( L L$+u#$n S#o Vi#$min B6 (8% ,

Dim$su$n 1$l$m l$3u #$$+ 1$n 1i#$m3$h$n $i+ $1 %( mL,



11/39


12/39

Di3$7$ $3so+3$nsi p$1$ m$C INH '*6! nm& 1$n m$C Vi#$min B6 '-*08! nm&,

Dim$su$n 1$#$ $3so+3$nsi #e+se3u# e 1$l$m * pe+s$m$$n .$n" 1i1$p$#,

Diseles$i$n pe+s$m$$n #e+se3u# 1en"$n me#o1e elimin$si un#u men7$+i $1$+ sen.$/$

.$n" 1i$n$lisis,

HASIL DAN PEMBAHASAN

Senyawa Vitamin B6 Senyawa INH

220 pada 254 nm

425 pada 324 nm

320 pada 266 nm


13/39


14/39

)*+ '9

INH

INH

0(001 *

0(001 *

0(374

0(372

0(037

0(045

Vi#, B6

Vi#, B6

0(002 *

0(002 *

0(2

0(315

0(752

0(771

;ata


15/39

D$#$ Repli$si

Berat )m&+

)!+

Berat )m&+

)B+

Berat )m&+

)+

Berat kerta# 0(2682 0(2670 0(2650

Berat kerta# - .at 0(3682 0(3672 0(3655

Berat kerta# - .at

#i#a0(264 0(2672 0(2654

Berat .at 0(0 0(1000 0(1001

!dd10 m$

$arutan yan&

di'aa 25

$ ad 10 m$

=p400

Nilai !'#%r'an#i )!+

ada m

a 1 )265(0

nm+

ada m

a 2 )324(5

nm+

; I ! 0(631> 0(727 0(035> 0(040

; II B 0(67> 0(55 0(032> 0(02

; III 0(746> 0(575 0(041> 0(026

R$#$5+$#$ :

m$C %

R I (86=;

R II (86%; *6! nm

R III (866%

m$C %

R I (8(-=!


16/39

R II (8(-(! -*08! nm

R III (8(--!

Konsen#+$si $/$lA A =!80= 3@ B 5%8(-6 , %(

50 3@

B B 5! 3@3 A =)8!0 3@

A =08-% 3@3 B 508%00 3@3

PERHITUN?AN

O+ien#$si

(86-* -=- A %!-8! B -)%

(8(0* 0% A -)% B %!-8!

*0(8=;* %0*,%%- A !),0)-8! B

6800= 6,00=

A !),0)-8! B J

*0-8-0! %-!666 A

A %8=*= %(5- 3@ 0((

A (86;% 3@ %((

%(( 6)86* 3@3

(8(0* 0% A -)% B

(8(0* 0% '%8=*= %(5-& -)% B

(8(0* (8(=% -)% 73

B 5=86%* %(5! 3@

%((

5=8!!; %(5! 3@3


17/39

Repli$si I

(86-* -=- A %!-8! B -)%(8(-=! 0% A -)% B %!-8!

*!)86;; %0*,%%- A !),0)-8! B

!8=!6*! 6*;-8

! A !),0)-8! B J

*!*8;0- %-!0%;8! A

A %8)6) %(5- 3@ 0((

A %(( =!80=

%(( 6)86* 3@3

(8(-=! 0% '%8)6) %(5-& -)% B

(8(-=! (8(=6!) -)% B

5(8(-;() -)% B

5%8(*!= %(50

B5%8(*!= %(50 3@ B

5%(-6 %(50 3@3

508%00 3@3

Repli$si II

(86=; -=- A %!-8! B -)%

(8(-(! 0% A -)% B -=-

*=8)0 %!*;- A 6*;-8! B

%%8-) %!*8;

- A %0*%%- B J


18/39

%6806 5%-!)%;8! B

B 5%8*%%; %(50 3@ 0((

(8(! 3@ (8(! 3@3 %(( 5! 3@3

(8(-(! 0% A -)% B

(8(-(! 0% A -)% '5%8*%%; %(50 &

(8(-(! 0% A '5(8(!&

A %8;6- %(5- 3@

%(( (8%;6 0((

=)8!0 3@3

Repli$si II

(866% -=- A %!-8! B -)%

(8(--! 0% A -)% B -=-

*!%8)0% %0*%%- A 6*;-8! B!8%0**! 6*;-8

! A %0*%%- B J

*!%8)-!) %-!0%;8! A

A %8)!;6 %(5- 3@

A %(( 5! 3@3

(8%)! 0(( =08-% 3@3

(8(--! 0% '%8)!;6 %(5-& -)% B

(8(--! (8(=6 -)% B

5%8%%! %(50 B


19/39

5%8%%! %(50 B

B %(( 50806 3@3

K$1$+ INH +$#$5#+$#$

=68%(= 3@3K$1$+ Vi#$min B6 +$#$5+$#$

5-8(0)

3

@3EVALUASI HASIL

x x d? < d2

75(47

78(54

74(31

76(107

0(637

2(433

1(77

0(406

5(1

3(22

@umla d 4(867 d2 (554

1 %86**

SD *8%)6

D$#$ .$n" 1i7u+i"$i =)8!0 3@3

H$+"$ 1i#ol$ 4i$ *8!

%8! *8! =)8!0 3@3 (Jadi harga diterima)

P (8;!G n -G # *8-!-


20/39

K$1$+ INH x #

=68%(= *8-!-

'=68%(= *8;6;=%& 3@3

K$1$+ Vi#$min B6

x x d? < d2

1(036 A 10


21/39

5-8(0) *8-!-

'5-8(0) -86--& 3@3

PEMBAHASAN

P+$#ium ini 1il$u$n un#u mene#$pn$n $1$+ sen.$/$ 1$l$m s$mpel .$n"

men"$n1un" 1u$ ompone4 $#$u le3ih8 men""un$$n $l$# spe#+oo#ome#e+ UV 1en"$n

me#o1e pe+s$m$$n simul#$n,

P+$#iu ini men""un$$n me#o1e pe+s$m$$n simul#$n $+en$ me#o1e ini 1$p$#men"uu+ le3ih 1$+i s$#u omponen 1$l$m sen.$/$8 .$n" mempun.$i se+$p$n p$1$

p$n4$n" "elom3$n" .$n" 3e+1e$#$n8 h$l ini 1$p$# #e+4$1i $+en$ me#o1e pe+s$m$$n

simul#$n 1il$u$n 1en"$n men"uu+ p$1$ * $#$u le3ih p$n4$n" "elom3$n" .$n" m$n$

m$sin"5m$sin" omponen #i1$ $$n men"$n""u, K+omoo+ .$n" 3e+3e1$53e1$ 1$+i #i$p

omponen .$n" $$n mempun.$i eu$#$n $3so+3s 7$h$.$ .$n" 3e+3e1$ pul$ p$1$ s$#u


22/39

1$e+$h p$n4$n" "elom3$n", Te#$pi #i1$ 1$p$# 1i"un$$n un#u sen.$/$ .$n"

mempun.$i "u"us +omoo+ s$m$,

S$mpel .$n" 1i"un$$n 1$l$h #$3le# .$n" men"$n1un" INH 'Isoni$9i1& 1$n

Vi#$min B6, INH 1$n Vi#$min B6 mempun.$i "u"us +omoo+ .$n" 3e+3e1$8 m$$ 1$#$

1i#e#$p$n $1$+n.$ men""un$$n me#o1e simul#$n, K+omoo+ $1$l$h "u"us $#$u s.s#em

.$n" 3e+#$n""un" 4$/$3 p$1$ pen.e+$p$n sin$+ .$n" me+up$$n "u"us #i1$ 4enuh .$n"

1$p$# men.e+$p +$1i$si 1$l$m 1$e+$h UV5Vis, Pen.e+$p$n se4uml$h ene+"i #e+se3u# $$n

men"h$sil$n #+$nsisi ele7#+on 1$n o+3i#$l 3e+esi#$si, Esi#$si $1$l$h #+$nsisi $#$u

pe+pin1$h$n ele7#+on 1$+i o+3i#$l +en1$h e o+3i#$l .$n" #in""i,

I9oni$9i1 mempun.$i h$+"$ 1$l$m pel$+u# $i+ $1$l$h -)( p$1$ *66

nm, 2$n" $+#in.$ Isoni$9i1 1en"$n $1$+ % 3@1i3$7$ $3so+3$nsin.$ p$1$ *66 nm

1en"$n ue# se#el$h % 7m $$n 1i1$p$# $3so+3$nsin.$ -)(, Se1$n"$n Vi#$min B6

mempun.$i h$+"$ 1$l$m pel$+u# $i+ **( p$1$ *!0 nm 1$n 0*! p$1$

-*0 nm,

L$+u#$n s#o 3$u INH 1$n Vi#$min B6 su1$h #e+se1i$ 1en"$n $1$+ %

3@1en"$n m$sin"5m$sin" pel$+u# $u$1es#, P$1$ penen#u$n m$C8 3e+1$s$+$n

h$+"$ 9$#8 un#u men"hi#un" 3e+$p$ onsen#+$si .$n" 1i"un$$n8 1ipe+oleh

onsen#+$si l$+u#$n INH .$n" 1i"un$$n $1$l$h (8((% 3@1$n 1i1$p$# m$C -*08!

nm 1en"$n $3so+3$nsi (8=0), Se1$n"$n onsen#+$si Vi#$min B6 .$n" 1i"un$$n $1$l$h

(8((* 3@1$n 1i1$p$# m$C *6!8( nm 1en"$n $3so+3$nsi (8-%*, Penen#u$n m$C

ini 1il$u$n p$1$ *(( nm < 0(( nm un#u UV,

P$1$ p+$#ium8 pem3$7$$n $3so+3$nsi 1il$u$n p$1$ m$C$+en$

epe$$nn.$ 4u"$ m$sim$l8 pe+u3$h$n $3so+3$nsi un#u se#i$p s$#u$n onsen#+$si

$1$l$h .$n" p$lin" 3es$+8 1i sei#$+ m$C8 3en#u u+$ $3so+3$nsin.$ 1$#$+ 'houm

L$m3e+#5Bee+ $$n #e+penuhi& 1$n 4i$ 1il$u$n pen"uu+$n ul$n" m$$ esl$h$n .$n"

ise3$3$n oleh pem$s$n"$n ul$n" p$n4$n" "elom3$n" $$n e7il se$li e#i$

1i"un$$n m$C,

P$1$ me#o1e pe+s$m$$n simul#$n8 pen"uu+$n 1il$u$n p$1$ m$sin"5m$sin"

l$+u#$n p$1$ #i$p p$n4$n" "elom3$n"8 sehin""$ 1ipe+oleh pe+s$m$n hu3un"$n $n#$+$


23/39

$3so+3$nsi 1en"$n onsen#+$si, Dil$u$n pem3$7$$n $3so+3$nsi sen.$/$ INH 1en"$n

$1$+ (8((% 3@p$1$ m$C % '*6! nm& 1ipe+oleh $3so+3$nsi (8-=0 1$n m$C *

'-*08! nm& 1ipe+oleh $3so+3$nsi (8(-=, Pem3$7$$n 1il$u$n 1u$ $li, Den"$n $1$+ INH

(8((% 3@1i3$7$ p$1$ m$C % '*6! nm& 1ipe+oleh $3so+3$nsi (8-=* 1$n m$C *

.$i#u -*08! nm (8(0!, Dipe+oleh +$#$5+$#$ $3so+3$nsi un#u INH (8((% 3@ m$C %

'*6! nm& $1$l$h (8-=- 1$n p$1$ m$C * '-*08! nm& 1ipe+oleh $3so+3$nsi (8(0%, Un#u

pem3$7$$n $3so+3$nsi Vi#$min B6 $1$+ (8((* 3@p$1$ m$C % '*6! nm& 1ipe+oleh

$3so+3$nsi (8*;; 1$n p$1$ m$C * '-*08! nm& 1ipe+oleh $3so+3$nsi (8(=!*, Pem3$7$$n

1il$u$n * $li sepe+#i INH 1$n 1ipe+oleh $3so+3$nsi Vi#$min B6 p$1$ m$C % '*6!

nm& $1$l$h (8-%! 1$n p$1$ m$C * '-*08! nm& $1$l$h (8==%, D$n 1ipe+oleh $1$+ +$#$5

+$#$ $3so+3$nsi Vi#$min B6 p$1$ m$C % '*6! nm& $1$l$h (8(8-(= 1$n p$1$ m$C *

'-*08! nm& $1$l$h (8=6*, Be+1$s$+$n houm L$m3e+#5Bee+ .$n" men.$#$$n 3$h$/$

in#ensi#$n .$n" 1i#e+us$n oleh l$+u#$n 9$# pen.e+$p 3e+3$n1in" lu+us 1en"$n #e3$l 1$n

onsen#+$si l$+u#$n,

A , 3 , 7

Sehin""$ 1ie#$hui 3$h/$ $3so+3$nsi 'A& 3e+3$1in" lu+us 1en"$n $3so+#ii#$s ' &8

#e3$l ue# '3&8 1$n onsen#+$si '7&, sup$.$ nil$i 3 #e#$p8 m$$ sel$m$ pen"uu+$n

1i"un$$n ue# 1en"$n e#e3$l$n .$n" s$m$, D$l$ houm L$m3e+#5Bee+ #e+1$p$#

3e3e+$p$ pem3$#$s$n .$i#u su$+ 1i"un$$n 1i$n""$p mono+om$#is8 pen.e+$p$n #e+4$1i

1$l$m su$#u oume .$n" mempun.$i pen$mp$n" lu$s .$n" s$m$8 sen.$/$ .$n"

men.e+$p 1$l$m l$u#$n #e+se3u#8 1$n #i1$ #e+4$1i pe+is#i/$ luo+osensi 1$n oso+esensi,

D$n 1i1$p$# pe+s$m$$n :

A p$1$ *6! nm : -=- A %!-8! B ,,,,,,,,, pe+s$m$$n '%&

A p$1$ *6! nm : 0% A -)% B ,, pe+s$m$$n '*&

Kemu1i$n 1iuu+ $3so+3$nsi s$mpel8 #e#$pi $+en$ s$mpel 1$l$m 3en#u #$3le#

m$$ 3e+$# s$#u #$3le# 1i#im3$n" emu1i$n 1i"e+us 1$n 1i3u$# l$+u#$n #e+le3ih 1$hulu,

Den"$n 7$+$ s$mpel 1i#im3$n" %((8( m" 1en"$n ses$m$ .$n" 1il$u$n p$1$ ne+$7$

$n$li#i epe$$n (8% m", menim3$n" ses$m$ .$n" 1im$su1 $1$l$h 1ei$si


24/39

penim3$n"$n #i1$ 3oleh le3ih 1$+i (8% 1$+i 3e+$# 9$# .$n" 1i#im3$n", Sel$n4u#n.$

1il$u$n 1en"$n pel$+u# .$n" s$m$ 1en"$n l$+u#$n s#o 3$u .$i#u $u$1es# 1$l$m l$3u

#$$+ %(8( mL emu1i$n 1is$+in" sup$.$ l$+u#$n 4e+nih8 $+en$ 3il$ #i1$ 4e+nih 3is$

#e+4$1i pen"h$m3u+$n /$+n$ p$1$ s$$# pem3$7$$n $3so+3$nsin.$,

P$1$ pem3$7$$n $3so+3$nsi8 l$+u#$n 1i$m3il *!L 1$n 1i#$m3$h$n $u$1es# $1

%(8( mL 1$l$m l$3u #$$+, Dil$u$n o+ien#$si p$1$ pem3$7$$n m$C % '*6! nm&

1i1$p$# $3so+3$nsi (86-*8 p$1$ m$C * '-*08! nm& 1i1$p$# $3so+3$nsi (8(0*,

P$1$ m$C % '*6! nm&8 $3so+3$nsi +$#$5+$#$ 1$+i e#i"$ +epli$si $1$l$h (86=;G (86%;G

(866%, P$1$ m$C * '-*08! nm& $3so+3$nsi +$#$5+$#$ 1$+i e#i"$ +epli$si $1$l$h

(8(-=!G (8(-(!G (8(--!, Den"$n pe+s$m$$n se3elumn.$ 1$p$# 1ihi#un" onsen#+$si s$mpel,

Konsen#+$si +$#$5+$#$ INH 1$l$m s$mpel .$n" 1i1$p$# $1$l$h =68%(= 3@31$n onsen#+$si

Vi#$min B6 +$#$5+$#$ 1$l$m s$mpel $1$l$h 5-8(0) 3@3, h$sil .$n" 1ipe+oleh Vi#$min B6

1$l$m s$mpel $1$l$h ne"$#i8 m$$ 1imun"in$n 1$l$m s$mpel $1$+ $ein su1$h

3e+u+$n" $#$u 3$h$n su1$h #i1$ $1$, Te#$pi h$sil ini 1$p$# 4u"$ 1imun"in$n $+en$

$1$ 3e3e+$p$ $7#o+ es$l$h$n 1$l$m p+$#ium sepe+#i menim3$n" s$mpel u+$n" #eli#i8

p$1$ s$$# pem3u$#$n l$+u#$n un#u 1i3$7$ $3so+3$nsin.$8 men"$1$nn.$ u+$n" #ep$#8

1$n p$1$ s$$# pen"$m3il$n l$+u#$n 1en"$n mi+opipe# u+$n" 3en$+, P$1$ s$$#

pem3$7$$n $3so+3$nsi semu$ l$+u#$n8 1i"un$$n l$+u#$n 3l$no .$i#u $u$1es# 's$m$

sepe+#i pel$+u#&, K$+en$ 3l$no 3e+un"si un#u mene#+$l$n $#$u men"hil$n"$n$3so+3$nsi 1$+i pel$+u# 1$l$m s$mpel8 sehin""$ .$n" #e+3$7$ p$1$ $l$# $3so+3$n h$n.$

$3so+3$nsi 1$+i s$mpel #$np$ $1$ "$n""u$n 1$+i pel$+u#,

D$+i e$lu$si h$sil 1i1$p$#$n $1$+ INH 1$l$m #$3le# #e+se3u# $1$l$h '=68%(=

*8;6;=& 3@31$n un#u $1$+ Vi$min B6 1$l$m #$3le# $1$l$h '5-8(0) -86--& 3@3,

KESIMPULAN

%& S$mpel men"$n1un" INH 1$n Vi#$min B6 1$p$# 1i#e#$p$n 1$+n.$ 1en"$n me#o1e

pe+s$m$$n simul#$n8 $+en$ INH 1$n Vi#$min B6 mempun.$i "u"us +omoo+,*& H$si ne"$#i p$1$ Vi#$min B6 1$p$# 1i$+#i$n 3$h/$ m$sih #e+3$7$n.$ INH p$1$

pem3$7$$n $+en$ mempun.$i .$n" #i1$ 3e"i#u 4$uh,

-& D$+i h$sil pe+7o3$$n 1ipe+oleh $1$+ 1$l$m s$mpel

INH '=68%(= *8;6;=& 3@3

Vi#$min B '5-8(0) -86--& 3@3,


25/39

DAFTAR PUSTAKA

Anonim, %;=;, Farmakope Indonesia Edisi III, Depes RI, >$$+#$

Au#e+ho 1$n Ko$+, *((*,Identifikasi Obat, ITB, B$n1un", %6!

?$n14$+8 I3nu ?holi3 1$n Rohm$n, *((=,Kimia Farmasi nalisi, Pus#$$ Pel$4$+, 2o".$$+#$, *0;

Ro#h8 He+m$nn, >, 1$n Bl$s7he8 ?o##+ie1, %;)),nalisi Farm asi,U?M, 2o".$$+#$, -6)5-6;

PENETAPAN KADAR TABLET ISONIAID DAN P2RIDOINE MEN??UNAKAN

SPEKTROFOTOMETRI UV DEN?AN METODE SIMULTAN

I, TU>UAN PEROBAAN

ntukmen&etauiarapenetapankadarta'let i#%nia.id dan pyrid%ine

men&&unakan#pektr%C%t%metriu9den&anmet%de#imultan

II, DASAR TEORI;adia#ielektr%ma&netik( yan& mana#inar ultra9i%let

dan#inartampakmerupakan#ala#atunya( dapatdian&&ap#e'a&aiener&i yan&

meram'atdalam'entuk&el%m'an&

Be'erapai#tiladanu'un&andi&unakanuntukmen&&am'arkan&el%m'an&ini

anDan&&el%m'an&merupakanDarak linear dari#uatutitikpada#atu&el%m'an&ketitik

yan& 'er#e'elaanpada&el%m'an& yan& 'erdekatan)I'nuE%li'EinanDardan

;%man(2007+

Biladiin&inkanpen&ukurandua'ua#enyawa#eara'er#ama F

#amapada#pektr%C%t%metri( makadapatdilakukanpada 2 panDan&&el%m'an& yan&

manama#in& F ma#in&k%mp%nentidak#alin&men&&an&&uatau&an&&uandarik%mp%nen

yan& alin palin& keil ua'uakr%m%C%r yan&

'er'edaakanmempunyaikekuatana'#%r'#iaaya yan& 'er'eda pula

pada#atudaerapanDan&&el%m'an& en&ukurandilakukanpadama#in& F


26/39

ma#in&larutanpada 2 panDan&&el%m'an&( #ein&&adiper%le 2

per#amaanu'un&anantaraa'#%r'an#iden&ank%n#entra#ipadaduapanDan&&el%m'an&(

aki'atnyak%n#entra#ima#in& F

ma#in&k%mp%nendapatdiitun&)I'nuE%li'EinanDardan ;%man(2007+

adaper%'aanini'ertuDuanuntukmenetapkankadar INH dalam#ediaan ta'let

yan& men&andun& INH


27/39

di#e'a'kan%lepilantiamil( yan& manadikiraadau'un&annyadena&nkekuran&an

#er%t%nin di %takaki'at meta'%li#m tript%Can yan& dipertin&&i)=e##enden(17+ntuk#uatularutan yan& men&andun& 2 k%mp%nen yan& menyerap( dan y(

#erapandiukurpada 2 panDan&&el%m'an& ,etelitiandantin&&i di

dapatkanden&anmemilipanDan&&el%m'an&dimanapanDan&&el%m'an&pen&ukuranmer

upakanpanDan&&el%m'an&dimana#erapanyamak#imal(

karenaden&anper&e#eran#edikitpadakur9a#erapantidakmenye'a'kanperu'aan#erapa

n yan& terlampauDau)wwwdi&ili'uiaid+

@umlak%mp%nendalamampurandapatmenapai 8

k%mp%nenden&an#yarat#eli#ipanDan&&el%m'an&mak#imumantarak%mp%nen minimal

5 nm @ikaDumlak%mp%nendalam#ampelle'idari 3( makauntukmen&itun&kadar di

&unakan #%Ctware multik%mp%nen yan& terdapatpadaalat#pektr%C%t%metri V


28/39

dilakukanden&anmem'uatkur9au'un&antaraa'#%r'an#iden&anpanDan&&el%m'an&dar

i#uatularutan'akupadak%n#entra#itertentu)I'nuE%li'EinanDardan ;%man(2007+

III, SIFAT BAHAN

Isoni$9i1@INH 'M1 -6 h$l *))&

;umu#m%lekul :Bm :137(1

emeriaan :tidak'erwarna( putitidak'er'au (,ri#tal atau'u'uk ,ri#tal puti

,elarutan : 1:8 air( 1:50 alk%%l( #edikitlarutdalamkl%r%C%m(

#an&at#edikitlarutdalameter

P.+i1oCine @i#$min B6 'M1 -6 h$l %;=)&

;umu#m%lekul :Bm :205(6

emerian :putiprakti#puti(,ri#tal 'u'uk ,ri#tal

,elarutan : 1:5 )air+( 1:115 )alk%%l+( tidaklarutdalameter

Isoni$9i1 'AOA8 p,*!*&

I#%nia.id $am'da Ga !1* 1m S%l9ent

I#%nia.id 265 420 0(01 N Hl

I#%nia.id 263 370 5* tH

I#%nia.id 267 374 0(5 N H2S4I#%nia.id 26 22 0(5 N NaH

P.+i1oCine Hl 'AOA8 p,*!;&yrid%ine Hl $am'da Ga !1* 1m S%l9ent

yrid%ine Hl 288 345 5* tH

yrid%ine Hl 20 425 0(1 N Hl

yrid%ine Hl 324( 254 350( 180 H2

elarutterpili 5*tH

Ren#$n"$3so+3$nsi (8! < %8!

I#%nia.id

0,2

370x10000

1,5

370x10000

5(4 ppm 40(5 ppm

;entan&terpili 5(4< 40(5


29/39

yrid%ine

0,2

345x10000

1,5

345x10000

5(8 ppm 43(5 ppm

;entan&terpili 5(8


30/39

6 J2244ppm

0,16

10=35,90ppm

ara kerDa :

ipetlarutan'akuinduk )30 Kl( 50Kl( 70Kl( 130Kl( 160Kl+

Ga#in& F ma#in&ditam'akanetan%l ad 10 ml( eka'#%r'an#inya

L$+u#$n3$uin1u p.+i1oCine

Konsentrasi :50,4 mg

50ml 1000=1008ppm

ara kerDa:

Tim'an& 50 m& pyrid%ine

$arutkandalamla'utakar 50 ml den&anetan%l ad 50 ml

,%%k ad %m%&en

$aruan'aku :

1 J1008ppm

0,03

10=3,02ppm

2J1008 ppm

0,05

10=5,04 ppm

3J1008ppm

0,07

10=7,06ppm

4J1008ppm

0,1

10=10,08ppm


31/39

5J1008ppm

0,13

10=13,10ppm

6 J

1008ppm0,16

10

=16,13ppm

ara kerDa :

ipetlarutan'akuinduk )30 Kl( 50Kl( 70Kl( 130Kl( 160Kl+

Ga#in& F ma#in&ditam'akanetan%l ad 10 ml( eka'#%r'an#inya

P+ep$+$sis$mpel

ara kerDa :

Tim'anampel#e'anyak 25 m&

Tam'akanetan%l ad 25 ml dalamla'utakar 25 mll

Sarin&den&ankerta#watman

ipet 0(3 ml )300 Kl+ den&anmikr%pipet

Tam'akanetan%l ad 10 ml dalamla'utakar )30 ppm+

Bo3o# +$#$ < +$#$ - #$3le#1,6901

3=0,56 g 563mg

I#%nia.id x

400563=25mg

x=17,76mg


32/39

yrid%ine x

10563=25mg

0(44 m&

Penim3$n"$ns$mpel

Sampel I 0(0268 &

Sampel II 0(026 &

Sampel III 0(0258 &

Pe+hi#un"$nonsen#+$sis$mpel

Sampel I 26,8mg

25ml 1000=1072ppm

1072 0,3ml

10ml 32(16

Sampel II 26,9mg

25ml 1000=1076ppm

1076 0,3ml

10ml 32(28

Sampel III 25,8mg

25ml 1000=1032ppm

1032

0,3ml

10ml 30(6

T$3elpen"$m$#$n3$u Isoni$9i1 'INH&


33/39

,%n#entra#i !'# L 1 I#%nia.id

)265(5+

!'# L2 yrid%ine

)286(5+

!1*1m I#%nia.id !1*

1m yrid%ine

6(73 0(183 0(07 271(2 117(38

11(22 0(31 0(318 284(31 283(42

15(71 0(45 0(204 22(17 12(85

22(44 0(633 0(275 282(0 122(55

2(17 0(826 0(367 283(17 125(81

35(30 0(53 0(432 265(46 120(33

R$#$5+$#$ *=;8)! R$#$5+$#$ %0;8);

Isoni$9i1p$1$

isoni$9i1

C1=0,183

6,7310.000

271(2

C2=0,319

11,22

10.000

284(31

C3=0,459

15,7110.000

22(17

C4=0,633

22,4410.000

282(0

C5=0,826

29,1710.000

283(17

C6=0,953

35,9010.000

265(46

Isoni$9i1p$1$

p.+i1oCine

C1=0,079

6,7310.000

117(38

C2=0,138

11,2210.000


34/39

283(42

C3=0,204

15,7110.000

12(85

C4=0,275

22,4410.000

122(55

C5=0,367

29,1710.000

125(81

C6=0,432

35,9010.000

120(33

T$3elpen"$m$#$n B$u P.+i1oCine

,%n#entra#i !'# L 1 I#%nia.id

)265(5+

!'# L2 yrid%ine

)286(5+

!1*1m I#%nia.id !1*

1m yrid%ine

3(02 0(014 0(057 46(36 188(74

5(04 0(028 0(110 55(55 218(25

7(06 0(060 0(184 84(5 260(62

10(08 0(083 0(238 82(34 236(11

13(10 0(104 0(311 7(3 237(40

16(13 0(153 0(41 4(85 25(76

R$#$5+$#$ =-8;% R$#$5+$#$ *--80)

P.+i1oCine p$1$

isoni$9i1

C1=0,014

3,0210.000

46(36

C2=0,028

5,04 10.000

55(55

C3=0,060

7,0610.000

84(5

C4=0,083

10,0810.000

82(34

C5=0,104

13,10

10.000

7(3

C6=0,153

16,1310.000


35/39

4(85

P.+i1oCinep$1$

p.+i11oCine

C1=0,0573,02

10.000

188(74

C2=0,110

5,0410.000

218(25

C3=0,184

7,0610.000

260(62

C4=

0,238

10,08 10.000

236(11

C5=0,311

13,1010.000

237(40

C6=0,419

16,1310.000

25(76


36/39

T$3elPen"$m$#$ns$mpel

,%n#entra#i !'# L 1I#%nia.id )265(5+ !'# L 1I#%nia.id )286(5+

32(16 0(617 0(2732(28 0(430 0(15

30(6 0(270 0(270

Sampel1:0,617=279,85INH . CINH+73,91PR. CPRx 233,48

0,279=149,89INH .CINH+233,48PR. CPRx73,91

144,05716=65339,378INH .CINH+17256,5068PR

20,62089=11078,3699INH .CINH+17256,5068PR

123,43627=54261,0081INH . CINH

CINH=2,275103 10.000=22,75ppm

CPR=

Sampel2:0,430=279,85INH . CINH+73,91PR. CPRx233,48

0,195=149,89INH .CINH+233,48PR. CPRx73,91

100,3964=65339,378INH . CINH+17256,5068PR

14,41245=11078,3699INH . CINH+17256,5068PR


37/39

85,98395=54261,0081INH . CINH

CINH=1,585103

10.000=15,85ppm

CPR=

Sampel3:0,600=279,85INH . CINH+73,91PR .CPRx 233,48

0,270=149,89INH .CINH+233,48PR . CPRx73,91

140,088=65339,378INH .CINH+17256,5068PR

19,957=11078,3699INH . CINH+17256,5068PR

120,1323=54261,0081INH . CINH

CINH=2,213103

10.000=22,13ppm

CPR=

Pe+hi#un"$n$1$+ isoni$9i1

Sampel 1 22,75

32,16x100

70(74 *

Sampel 2 15,85

32,28x100

4(10 *


38/39

Sampel 3 22,13

30,96x100

71(47 *

Pe+hi#un"$n3e+$#

Sampel 1 70(47 * 563 36( 75

Sampel 2 4( 10 * 563 276(43

Sampel 3 71(47 * 563 402(37

Pe+hi#un"$n 01

,adar )*+ ;ata


39/39

tidakadapartikelk%l%idapala&i#u#pen#i(

karenaapa'ila#ampeltidakDerniataudikatakanterdapat#enyawa lain yan&

tidaklarutmakaa#ilpem'aaana'#%r'an#imenDaditidaktepat

adapenetapankadarkap#ulkl%ramCenik%linipraktikanmelakukanprepara#i#ampelden&anmet%deCil

tra#iatau di di#arin&men&&unkankerta##arin&

adapraktikuminikel%mp%k kami mendapatkana#ilDumla i#%nia.id 3(56 m& ta'let

#edan&kankadar#e'enarnyaadala 400 m&ta'let #edan&kankadarpyrid%k#inetidakter'aa

,e#alaandalampenetapankadarinidi#e'a'kankarenakelarutanpyrid%yne#an&at'e#ardankadarpy

rid%yne yan& terlalukeilyaitu 10 m&ta'let #edankankadar i#%nia.id 400 m&ta'let

dankearutannyayaitu 1:115 dalametan%ldankelarutan i#%nia.id 1:50 dalametan%l

!ki'atdariper'edaankelarutandankadar yan& #i&niCikaninimakaada#eDumlapyrid%yne yan&

tidakterlarut#empurna#elainituDu&ama#iada yan& tertin&&aldalamla'uukurmaupundalam pr%#e#

penyarin&an#ein&&aketikadilakukanpenetapankadarmakatidakter'aa

apatdikatakanDu&a#emakinkeilkadar%'at yan&

akanditentukanmakaakan#emakin'e#arper#enke#alaan yan& didapat

KESIMPULAN

,adar pyrid%ine yan& didapatadala 3(56 m& ta'let

DAFTAR PUSTAKA

!n%nim 17 =armak%pe Ind%ne#ia di#i III epartemen ,e#eatan;epu'likInd%ne#ia :

@akarta

;%man( !'dul( I'nuEandDar 2007Kimia FarmasiAnalisis u#takaBelaDar : %&yakarta 2008

Kimia FarmasiAnalisis.u#takaelaDar : %&yakarta

=e##enden( ;alp @ dan @%an S =e##enden( 17( OKimia OrganikP( Dilid 1 edi#iketi&a(

terDemaan%le : !l%y#iu# H ( ener'itrlan&&a( @akarta

Gartindale )200+ Martindale The Complete Drug Reference. < 36t diti%n

edarmaeutial re##

Documents

spektrofotometri uv zat campuran